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Relationships between morphological descriptors and RAPD markers for assessing genetic variability in Hippophae rhamnoides L.

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Forestry Studies in China 2012年第2期14卷 137-144页

作者： Rachana RANA poonam shirkot Department of Biotechnology Dr.YS Parmar University of Horticulture and ForestryNauniSolan 173230(Himachal Pradesh)India

Knowledge of genetic relatedness among accessions of germplasm is necessary for the development of breeding strategies to produce improved cultivars. The present investigation on Hippophae rhamnoides was carried out to assess its genetic variability in Himachal Pradesh, India, by employing morphological and RAPD markers. Different areas of Himachal Pradesh were surveyed and eight sites were finally selected Twenty four genotypes were selected for further studies, i.e., three genotypes from each site. On the basis of morphological studies, the genotypes of the Ropa site （Kinnaur） were considered elite genotypes. To assess the variability at the molecular level, RAPD patterns were studied by random primers. The total number of bands amplified was 607, out of which 487 bands were identified as polymorphic, depicting 80.23 per cent variability. Six unique bands were produced from three primers （OPA- 05, OPA-10 and OPD-08） specific for five genotypes, including three genotypes of the Ropa site, Kinnaur District.

关键词： Hippophae rhamnoides morphological descriptors genetic variability RAPD-PCR

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Molecular identification of sex in Hippophae rhamnoides L. using isozyme and RAPD markers

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Forestry Studies in China 2010年第2期12卷 62-66页

作者： Amit SHARMA Gaurav ZINTA Satender RANA poonam shirkot Department of Biotechnology Dr. Yashwant Singh Parmar University of Horticulture & Forestry

In many dioecious plants, gender affects economic value, breeding schemes and opportunities for commercial harvests. Hippophae rhamnoides L. is a dioecious plant species in which female genotypes are commercially preferred over male genotypes. Its berries have rich medicinal, nutritional and pharmaceutical properties because of their large amounts of vitamins, essential oils, proteins, fatty acids, free amino acids and flavanoids. Primary limitation for breeding H. rhamnoides L. is its dioecious nature, since gender cannot be identified by traditional methods. Therefore, some reliable and quick methods need to be developed. This commu- nication deals with the development of isozyme and RAPD markers for early sex identification in this dioecious tree. The isozyme analysis was conducted with four enzyme systems, viz. peroxidase, esterase, malate dehydrogenase and catalase. The peroxidase enzyme system produced a female specific sex marker, which successfully differentiated between the staminate and pistillate geno- types ofH. rhamnoides L. Thirty five random decamer primers were used in our study and one male sex linked marker was identified. OPD-20 （5＇-ACTTCGCCAC-3＇） displayed a band at 911 bp that expressed polymorphism between male and female genotypes. The staminate and pistillate genotypes could be distinguished using RAPD marker OPD-209n. These results revealed the immense poten- tial of peroxidase isozyme patterns and RAPD as genetic markers for sex identification in H. rhamnoides L.

关键词： Hippophae rhamnoides L. sex markers isozyme RAPD dioecism

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