检索结果-南通市图书馆

番茄抗坏血酸含量相关QTL定位及候选基因鉴定

在线全文

学校读者我要写书评

暂无评论

园艺学报 2024年第2期51卷 219-228页

作者：刘根忠李方曼葛平飞陶金宝张星雨叶志彪张余洋山东农业大学园艺科学与工程学院山东泰安271018 华中农业大学果蔬园艺作物种质创新与利用全国重点实验室武汉430070 湖北洪山实验室武汉430070 华中农业大学深圳营养与健康研究院武汉430070 中国农业科学院深圳农业基因组研究所岭南现代农业科学与技术广东省实验室深圳分中心广东深圳518000

以番茄果实高抗坏血酸含量材料TS-226和低抗坏血酸含量材料TS-228为试验亲本杂交和自交创制F2遗传分离群体,分别构建高抗坏血酸混池和低抗坏血酸混池,集团分离群体测序(bulked segregant analysis sequencing,BSA-seq)分析鉴定到与番茄... 详细信息

以番茄果实高抗坏血酸含量材料TS-226和低抗坏血酸含量材料TS-228为试验亲本杂交和自交创制F2遗传分离群体,分别构建高抗坏血酸混池和低抗坏血酸混池,集团分离群体测序(bulked segregant analysis sequencing,BSA-seq)分析鉴定到与番茄果实抗坏血酸相关的主效QTL,进一步将与抗坏血酸相关的候选基因定位于番茄基因组8号染色体58.00~60.15 Mb区域内。结合?SNP-index数据,推断SlPPO(Polyphenol oxidase)是参与调控番茄抗坏血酸含量的主效基因。以TS-228为背景材料,通过农杆菌介导方法进行遗传转化,获得SlPPO过表达和沉默株系,相比野生型,过表达株系OE-3和OE-18的红熟果实总抗坏血酸含量分别减少了18.89%和26.56%;沉默株系Ri-9和Ri-16红熟果实总抗坏血酸含量分别增加了37.53%和63.93%。综上所述,SlPPO为参与控制番茄红熟果实抗坏血酸含量的关键基因,对果实抗坏血酸含量起负向调控作用。

关键词：番茄集团分离群体测序抗坏血酸 QTL SlPPO

A 21-bp InDel in the promoter of STP1 selected during tomato improvement accounts for soluble solid content in fruits

在线全文

学校读者我要写书评

暂无评论

Horticulture Research 2023年第3期10卷 176-188页

作者： Ying Wang Chunmei Shi pingfei ge Fangman Li Lihui Zhu Yaru Wang Jinbao Tao Xingyu Zhang Haiqiang Dong Wenxian Gai Fei Wang Zhibiao Ye Donald Grierson Wei Xu Yuyang Zhang National Key Laboratory for germplasm Innovation and Utilization of Horticultural Crops College of Horticulture and Forestry ScienceHuazhong Agricultural UniversityWuhan 430070China Plant Sciences Division School of BiosciencesUniversity of NottinghamSutton Bonington CampusLoughborough LE125RDUK Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and germplasm Resources Utilization(Xinjiang Production and Construction Crops) College of AgricultureShihezi UniversityShihezi 832003XinjiangChina Hubei Hongshan Laboratory Wuhan 430070China

Domestication and improvement are important processes that generate the variation in genome and phonotypes underlying crop ***,during selection for certain attributes,other valuable traits may be inadvertently *** example is the decline in fruit soluble solids content(SSC)during tomato *** genetic loci for SSC have been identified,but few reports on the underlying mechanisms are *** this study we performed a genome-wide association study(GWAS)for SSC of the red-ripe fruits in a population consisting of 481 tomato accessions with large natural variations and found a new quantitative trait locus,STP1,encoding a sugar transporter *** causal variation of STP1,a 21-bp InDel located in the promoter region 1124 bp upstream of the start codon,alters its ***1 Insertion accessions with an 21-bp insertion have higher SSC than STP1Deletion accessions with the 21-bp *** of STP1 in TS-23 with high SSC using CRISPR/Cas9 greatly decreased SSC in *** vivo and in vitro assays demonstrated that ZAT10-LIKE,a zinc finger protein transcription factor(ZFP TF),can specifically bind to the promoter of STP1Insertion to enhance STP1 expression,but not to the promoter of STP1Deletion,leading to lower fruit SSC in modern *** analysis revealed that STP1 was selected during tomato *** these results together,we identified a naturally occurring causal variation underlying SSC in tomato,and a new role for ZFP TFs in regulating sugar *** findings enrich our understanding of tomato evolution and domestication,and provide a genetic basis for genome design for improving fruit taste.

关键词： CRISPR/Cas9 content sugar

SIRCM1,which encodes tomato Lutescent1,is required for chlorophyll synthesis and chloroplast development in fruits

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Horticulture Research 2021年第1期8卷 1643-1656页

作者： genzhong Liu Huiyang Yu Lei Yuan Changxing Li Jie Ye Weifang Chen Ying Wang pingfei ge Junhong Zhang Zhibiao Ye Yuyang Zhang Key Laboratory of Horticultural Plant Biology Ministry of EducationHuazhong Agricultural UniversityWuhanChina

In plants,chloroplasts are the sites at which photosynthesis occurs,and an increased abundance of chloroplasts increases the nutritional quality of plants and the resultant color of ***,the molecular mechanisms underlying chlorophyll synthesis and chloroplast development in tomato fruits remain *** this study,we isolated a chlorophyll-de fi cient mutant,reduced chlorophyll mutant 1(rcml),by ethylmethanesulfonate mutagenesis;this mutant produced yellowish fruits with altered chloroplast *** revealed that Solyc08g005010 is the causal gene underlying the rcm1 mutant phenotype.A single-nucleotide base substitution in the second exon of SIRCM1 results in premature termination of its translated ***1 encodes a chloroplast-targeted metalloendopeptidase that is orthologous to the BCM1 protein of Arabidopsis and the stay-green G protein of soybean(Glycine max ***.).Notably,the yellowish phenotype of the lutescent1 mutant can be restored with the allele of SlRCM1 from wild-type *** contrast,knockout of SlRCM1 by the CRISPR/Cas9 system in Alisa Craig yielded yellowish fruits at the mature green stage,as was the case for *** acid sequence alignment and functional complementation assays showed that SlRCM1 is indeed *** fi ndings provide new insights into the regulation of chloroplast development in tomato fruits.

关键词： synthesis fruits alignment

Multiple-model GWAS identifies optimal allelic combinations of quantitative trait loci for malic acid in tomato

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Horticulture Research 2023年第4期10卷 23-37页

作者： Wenxian Gai Fan Yang Liangdan Yuan Saeed ul Haq Yaru Wang Ying Wang Lele Shang Fangman Li pingfei ge Haiqiang Dong Jinbao Tao Fei Wang Xingyu Zhang Yuyang Zhang National Key Laboratory for germplasm Innovation and Utilization of Horticultural Crops Huazhong Agricultural UniversityWuhan 430070China Hubei Hongshan Laboratory Wuhan 430070China College of Horticulture Northwest A&F UniversityYangling 712100China Department of Horticulture The University of Agriculture PeshawarPeshawar 25130Pakistan

Malic acid(MA)is an important flavor acid in fruits and acts as a mediator in a series of metabolic *** is important to understand the factors affecting MA metabolism for fruit flavor improvement and to understand MA-mediated biological ***,themetabolic accumulation of MA is controlled by complex heredity and environmental factors,making it difficult to predict and regulate the metabolism of *** this study,we carried out a genome-wide association study(GWAS)on MA using eight milestone models with two-environment repeats.A series of associated SNP variations were identified from the GWAS,and 15 high-confidence annotated geneswere further predicted based on linkage disequilibrium and lead *** transcriptome data of candidate geneswere explored within different tomato organs as well as various fruit tissues,and suggested specific expression patterns in fruit *** on the genetic parameters of population differentiation and SNP distribution,tomato MA content has been more influenced by domestication sweeps and less affected by improvement sweeps in the long-term history of tomato *** addition,genotype×environment interaction might contribute to the difference in domestication phenotypic data under different *** study provides new genetic insights into how tomato changed its MA content during breeding and makes available function-based markers for breeding by marker-assisted selection.

关键词： breeding GWAS metabolism

芪白平肺胶囊对慢性阻塞性肺疾病模型大鼠的保护作用

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国药房 2014年第19期25卷 1741-1743页

作者：张贺葛平王晓玉孟楣安徽中医药大学研究生院合肥230038 安徽中医药大学第一附属医院/国家中医药管理局中药制剂三级实验室合肥230031

目的:研究芪白平肺胶囊对慢性阻塞性肺疾病(COPD)模型大鼠的保护作用。方法:采用复合因素法复制大鼠COPD模型。90只SD大鼠随机均分为正常对照(等容生理盐水)组、模型(等容生理盐水)组、固本咳喘片(0.72 g/kg)组与芪白平肺高、中、低剂量... 详细信息

目的:研究芪白平肺胶囊对慢性阻塞性肺疾病(COPD)模型大鼠的保护作用。方法:采用复合因素法复制大鼠COPD模型。90只SD大鼠随机均分为正常对照(等容生理盐水)组、模型(等容生理盐水)组、固本咳喘片(0.72 g/kg)组与芪白平肺高、中、低剂量(2.88、1.44、0.72 g/kg)组,复制模型第31天开始灌胃给药,每天1次,连续30 d。测定大鼠支气管肺泡灌洗液(BALF)中细胞总数和分类计数,测定BALF中白细胞介素(IL)-8、IL-6和肿瘤坏死因子(TNF)-α的含量。结果:与正常对照组比较,模型组大鼠BALF中白细胞总数增加,中性粒细胞总数、淋巴细胞比例、中性粒细胞比例增加,单核-巨噬细胞比例减少,IL-6、IL-8、TNF-α含量增加,差异有统计学意义(P<0.01);与模型组比较,芪白平肺胶囊高、中、低剂量组大鼠BALF中白细胞总数、中性粒细胞比例、淋巴细胞比例减少,单核-巨噬细胞比例增加,IL-6、IL-8、TNF-α含量减少,差异有统计学意义(P<0.01或P<0.01)。结论:芪白平肺胶囊能下调COPD模型大鼠支气管BALF中IL-8、IL-6、TNF-α的含量,减轻气道炎症反应而发挥治疗作用。

关键词：慢性阻塞性肺疾病芪白平肺胶囊白细胞介素6 白细胞介素8 肿瘤坏死因子α

芪白平肺胶囊对大鼠慢性阻塞性肺疾病模型肺功能的影响

在线全文

学校读者我要写书评

暂无评论

中药新药与临床药理 2012年第6期23卷 623-626页

作者：孟楣李泽庚王晓玉肖伟姜辉尚伟葛平安徽中医学院第一附属医院合肥230031 安徽中医学院合肥230038 江苏康缘药业股份有限公司江苏连云港222002

目的研究芪白平肺胶囊对慢性阻塞性肺疾病(COPD)模型大鼠的肺组织作用。方法采用复合因素造模方法复制COPD大鼠模型,造模后给予低、中、高剂量的芪白平肺胶囊(0.72,1.44,2.88 ***-)1,给药30 d,观察大鼠的一般情况,测定各组大鼠第0.3 s... 详细信息

目的研究芪白平肺胶囊对慢性阻塞性肺疾病(COPD)模型大鼠的肺组织作用。方法采用复合因素造模方法复制COPD大鼠模型,造模后给予低、中、高剂量的芪白平肺胶囊(0.72,1.44,2.88 ***-)1,给药30 d,观察大鼠的一般情况,测定各组大鼠第0.3 s用力呼气量(FEV0.3)、FEV0.3与用力肺活量(FVC)的比值、呼气峰流速(PEF)、肺组织匀浆中白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)的含量,HE染色法观察大鼠肺组织病理学改变。结果与模型组比较,芪白平肺各剂量组大鼠FEV0.3、FEV0.3/FVC、PEF均明显升高,差异有统计学意义(P<0.01,P<0.05);芪白平肺各剂量组肺组织中IL-6、IL-8和TNF-α含量比模型组降低,差异有统计学意义(P<0.05,P<0.01)。结论芪白平肺胶囊对COPD大鼠的肺组织具有保护作用。

关键词：芪白平肺胶囊慢性阻塞性肺疾病肺功能

高效液相色谱法测定芪白平肺胶囊中人参皂苷R_(g1)与Rb_1含量

在线全文

学校读者我要写书评

暂无评论

安徽中医学院学报 2013年第5期32卷 72-75页

作者：王芳王斌张贺葛平李泽庚孟楣安徽中医药大学研究生部安徽合肥230038 安徽中医药大学第一附属医院国家中医药管理局中药制剂三级实验室安徽合肥230031

目的采用高效液相色谱法测定芪白平肺胶囊中人参皂苷Rg1与Rb1的含量，为其质量控制提供依据。方法采用WelchMaterialIncC18色谱柱（4．6mm×250mm，5μm）。其流动相：水-乙腈；梯度洗脱（0～10min，81：19；11～35min，81：19—71：29... 详细信息

目的采用高效液相色谱法测定芪白平肺胶囊中人参皂苷Rg1与Rb1的含量，为其质量控制提供依据。方法采用WelchMaterialIncC18色谱柱（4．6mm×250mm，5μm）。其流动相：水-乙腈；梯度洗脱（0～10min，81：19；11～35min，81：19—71：29；36～50min，71：29—50：50）；流速：1．2ml／min；检测波长：203nm；柱温：30℃。结果人参皂苷Rg1、Rb1在0．16～2．40μg范围内与其峰面积呈线性关系，r=0．9999（n=8）；人参皂苷Rg1、Rb1的平均加样回收率分别为102．31％、98．55%（n=9）；芪白平肺胶囊中人参皂苷Rg1、Rb1的3批样品测定结果，人参皂苷Rg1平均含量分别为0．332、0．326、0．336mg／g，人参皂苷Rb1平均含量分别为0．496、0．509、0．511mg／g。结论所建立的高效液相色谱方法简便、灵敏度高、重现性好，可用于芪白平肺胶囊中人参皂苷的含量测定。

关键词：高效液相色谱法梯度洗脱芪白平肺胶囊人参皂苷含量测定