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No longer ‘blob-ology': Cryo-EM is getting into molecular details

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Science China(Life Sciences) 2015年第11期58卷 1154-1156页

作者： zhu HongTao zhu ping National Laboratory of Biomacromolecules Institute of Biophysics Chinese Academy of Sciences

The structural information of functionally important mac- romolecular assemblies is a key to molecular biology and is of great interest to structural biologists. Cryo-electron mi- croscopy （cryo-EM）, particularly the single particle analysis （SPA）, has been regarded as one of the three primary tech- niques for structure determination, together with X-ray crystallography and nuclear magnetic resonance （NMR）. For single particle cryo-EM, a tiny amount （~3 μL） of puri- fied biological samples at a concentration around 0.1-2 mg mL-1 are placed onto an EM grid, blotted with a filter paper and rapidly frozen into a thin layer of vitreous ice. The EM grid with frozen samples is then transferred to a transmis- sion electron microscope for imaging.

关键词：分子生物学冷冻电镜透射电子显微镜单颗粒分析斑点结构信息 X射线晶体学生物学家

Optimal arrangement of viscoelastic dampers for seismic control of adjacent shear-type structures

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2013年第1期14卷 47-60页

作者： Xiao HUANG Hong-ping zhu School of Civil Engineering and Mechanics Huazhong University of Science and Technology Hubei Key Laboratory of Control Structure Huazhong University of Science and Technology

The optimal arrangement of viscoelastic dampers (VEDs) used to link two adjacent shear-type structures under seismic excitation was investigated. A two-step optimal design method is proposed. First, optimal parameter expressions of the Kelvin model are used to calculate the optimal stiffness and damping coefficient of the VEDs. Then, using the two-step optimal design method, taking the quadratic performance index as the optimization objective, the optimal arrangement of the dampers is determined. General rules about the optimal arrangement of the VEDs were obtained. The results show that the placement of only one damper between two adjacent shear-type structures should be avoided; if more than one damper is used, they should be distributed on the top and lower floors of the structures. Optimization of the number of dampers had little effect on response reduction. The most important factor was the optimization of the placement of the dampers. Through comparative study, for buildings of equal and unequal heights, the optimal parameters of dampers from parametric studies were shown to match the theoretical results for different numbers and placements of dampers. The level of response reduction was shown to be sensitive to the damping coefficient of the dampers.

关键词： Adjacent shear-type structures Viscoelastic damper (VED) Optimal arrangement Seismic response

Optimal approximate merging of a pair of Bézier curves with G^2-continuity

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2009年第4期10卷 554-561页

作者： ping zhu Guo-zhao WANG Institute of Computer Graphics and lmage Processing State Key Lab of CAD & CG Zhejiang University Hangzhou 310027 China

We present a novel approach for dealing with optimal approximate merging of two adjacent Bezier eurves with G^2-continuity. Instead of moving the control points, we minimize the distance between the original curves and the merged curve by taking advantage of matrix representation of Bezier curve＇s discrete structure, where the approximation error is measured by L2-norm. We use geometric information about the curves to generate the merged curve, and the approximation error is smaller. We can obtain control points of the merged curve regardless of the degrees of the two original curves. We also discuss the merged curve with point constraints. Numerical examples are provided to demonstrate the effectiveness of our algorithms.

关键词： Approximate merging Gl-continuity G^2-continuity Discrete subdivision Point constraints

Rabbet: Using Layout-Aware Backup for RAID Reconstruction

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Chinese Journal of Electronics 2017年第4期26卷 710-719页

作者： zhu Xudong XU Wei MAO Yuxin zhu ping Zhejiang Gongshang University Institute of Computing Technology Chinese Academy of Sciences

Once a disk of storage system is failed,RAID mechanism will reconstruct to keep the *** reconstruction leads to a sharp deterioration in I/O performance of RAID, and the storage system cannot provide high performance service for the applications. The Multi-TB disk capacity and high load of the application make the reconstruction last for a very long time. This paper proposes Rabbet, a method accelerating RAID reconstruction using data of backup. Rabbet includes a new RAID reconstruction frame, a RAID layout-aware mapping mechanism for backup, and a reconstruction algorithm. The result of experiments shows that, comparing to the classic DOR methods Rabbet shortens the RAID5 reconstruction process to 13.9%–42.8% and decreases the average response time for applications during reconstruction by 3.2%–14.6%. Rabbet also improves the reliability of storage systems.

关键词： RAID reconstruction Backup Layout aware

Origins and transports of the low-salinity coastal water in the southwestern Yellow Sea

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Acta Oceanologica Sinica 2018年第4期37卷 1-11页

作者： zhu ping WU Hui State Key Laboratory of Estuarine and Coastal Research East China Normal University School of Marine Sciences East China Normal University

In the southwestern Yellow Sea there is a low-salinity and turbid coastal water,the Subei Coastal Water（SCW）.The origins of freshwater contents and thus the dissolved terrigenous nutrients in the SCW have been debated for *** this study,we used a well-validated numerical model to quantify the contributions of multiple rivers,i.e.,the Changjiang River in the south and the multiple Subei local rivers（SLRs） in the north,in forming this yearround low-salinity coastal *** is found that the freshwater contents in the SCW is dominated by the Changjiang River south of 33.5°N,by the SLRs north of 34.5°N,and by both sources in 33.5°–34.5°***,the Changjiang River contributes ~70% in the dry season and ~80% in the wet season of the total freshwater contents in the SCW,*** driving the Changjiang River Plume to flow northward is the tidal residual current,which can even overwhelm the wind effects in winter *** residual currents turn offshore near the Old Yellow River Delta（OYRD） by the collision of the two tidal wave systems,which transport the freshwater from both sources into the interior Yellow *** age experiments show that it takes 50–150 d for the Changjiang River Plume to reach the SCW in the spring and summer seasons,thus there is a 2-month lag between the maximum freshwater content in SCW and the peak Changjiang River *** the winter and autumn seasons,the low salinity in inner SCW is the remnant Changjiang River diluted water arrived in the previous seasons.

关键词： Subei Coastal Water origins river plume numerical modeling

New insights into the helical structure of 30-nm chromatin fibers

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Protein & Cell 2014年第7期5卷 489-491页

作者： ping Chen ping zhu Guohong Li National Laboratory of Biomacromolecules Institute of Biophysics Chinese Academy of Sciences

In eukaryotic cells, genomic DNA is highly packaged into chromatin to fit inside the nucleus. The accessibility of DNA is dependent on the packing density of chromatin fibers,which plays a critical role in transcripti... 详细信息

关键词： chromatin ray/neutron centrifugation

Boundedness of Multilinear Caldern–Zygmund Singular Operators on Morrey–Herz Spaces with Variable Exponents

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Acta Mathematica Sinica,English Series 2014年第7期30卷 1180-1194页

作者： Yan LU Yue ping zhu School of Xinglin Nantong University School of Science Nantong University

In this paper, we introduce Morrey-Herz spaces MKq.p（·）α（·）,λ with variable exponents α（·） and p（·）, and prove the boundedness of multilinear Calderdn-Zygmund singular operators on the product of these... 详细信息

关键词： Morrey-Herz spaces variable exponents multilinear Calder6n-Zygmund singular operators

Clinical features of aortic dissection associated with Takayasu＇s arteritis

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Journal of Geriatric Cardiology 2017年第7期14卷 485-487页

作者： Xue-ping WU ping zhu Cardiovascular Department of Southern Building Chinese PLA General Hospital Beijing China

Takayasu＇s arteritis （TA） is a chronic and nonspecific in- flammatory vessel disease that involves the aorta and its major branches. TA results in stenosis, occlusion or aneu- rysmal degeneration of large arteries pathologically. Al- though TA is an unusual disease, it is more commonly ob- served to occur in Asian females compared to the general population worldwide. Aortic dissection is a very rare com- plication of TA, and only a limited number of TA cases presenting with aortic dissection have been published so far.

关键词： Aortic dissection CT Takayasu's arteritis

Rapid Time-Decay Phenomenon of the Incompressible Navier–Stokes Flow in Exterior Domains

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Acta Mathematica Sinica,English Series 2022年第4期38卷 745-760页

作者： Qing Hua ZHANG Yue ping zhu School of Sciences Nantong UniversityNantong 226019P.R.China Department of Mathematics Nantong Normal CollegeNantong 226010P.R.China

This paper focuses on the rapid time-decay phenomenon of the 3 D incompressible NavierStokes flow in exterior *** using the representation of the flow in exterior domains,together with the estimates of the Gaussian kernel,the tensor kernel,and the Stokes semigroup,we prove that under the assumption∫0∞∫ΩT[u,p](y,t).νdSydt=0 for the body pressure tensor T[u,p],if u0∈L1(Ω)∩Lσ3(Ω)∩W2/5,5/4(Ω)with‖u0‖3≤ηfor some sufficiently small numberη>0,then rapid time-decay phenomenon of the Navier-Stokes flow *** additionally|x|αu0∈Lr0(Ω)for some0<α<1 and 1

关键词： Incompressible Navier–Stokes equation exterior domain strong solution rapid time-decay phenomenon higher decay rates