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Comparison of the emission wavelengths by a single fluorescent dye on in vivo 3-photon imaging of mouse brains

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Journal of Innovative Optical Health sciences 2023年第6期16卷 23-30页

作者： Ke Wang Wanjian Zhang Xiangquan Deng Shen Tong hui Cheng Mengyuan Qin Lei zheng Kun Zhao Ruizhan zhai Zhongqing Jia ping Qiu Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province college of Physics and Optoelectronic Engineering Shenzhen University Shenzhen 518060 P.R.China Laser Institute Qilu University of Technology(Shandong Academy of sciences)Qingdao Shandong 266000 P.R.China

Multiphoton microscopy(MPM)is a powerful imaging technology for brain *** imaging depth in MPM is partly determined by emission wavelength of fluorescent *** has been demonstrated that a longer emission wavelength is favorable for signal detection as imaging depth ***,there has been no comparison with near-infrared(NIR)*** order to quantitatively analyze the effect of emission wavelength on 3-photon imaging of mouse brains in vivo,we utilize the same excitation wavelength to excite a single fluorescent dye and simultaneously collect NIR and orange-red emission fluorescence at 828 nm and 620 nm,*** experimental and simulation results show that as the imaging depth increases,NIR emission decays less than orange-red fluorescent *** results show that it is preferable to shift the emission wavelength to NIR to enable more e±cient signal collection deep in the brain.

关键词： 3-Photon microscopy emission fluorescence FM4-64 Monte Carlo.

Changes of spring wind erosion based on wind erosion climate factor in the black soil region of Northeast China

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Journal of Mountain Science 2023年第6期20卷 1712-1724页

作者： YAN ping JI Sheng-tai LI Xiu-fen ZHU Hai-xia WANG Liang-liang zhai Mo WANG ping ZHAO hui-ying Heilongjiang Institute of Meteorological sciences Harbin 150030China Innovation and Open Laboratory of Eco-meteorology in Northeast China China Meteorological AdministrationHarbin 150030China Heilongjiang Ecological Meteorological Center Harbin 150030China

The dry and windy climate and low ground cover in spring in the black soil region of Northeast China make the soil strongly affected by wind erosion,which seriously threatens the food security and ecological security of this *** this paper,based on the daily observation data of 124 meteorological stations in study area from 1961 to 2020,seasonal and monthly wind erosion climate factor(C)in spring(March to May)were calculated by using the method proposed by the Food and Agriculture Organization of the United Nations(FAO),the wind erosion characterization in spring were systematically analyzed based on C by various statistical analysis *** results showed that in the past 60 years,spring wind erosion climate factor(CSp)and monthly C of the whole region and each province(region)all showed highly significant decreasing trend,but they began to show rebounded trend in the middle or late *** of the study area showed a significant upward trend since 2008 with an increase of 4.59(10a)^(-1).The main contributors to this upward trend are the changes of C in March and in *** the four provinces(regions),CSp in Heilongjiang,Jilin,Liaoning and eastern Inner Mongolia all showed rebounded since 2008,2011,2008 and 2009,*** rebounded trend of CSp in eastern Inner Mongolia was the most obvious with a tendency rate of 11.27(10a)^(-1),and its mutation occurred after *** rebound trend of CSp in Heilongjiang Province takes the second place,with a trend rate of 4.72(10a)^(-1),but there’s no obvious time mutation *** spatial characteristics of CSpand monthly C are similar,showing decreasing characteristics centered on the typical black soil belt of Northeast *** with 1961-1990,in the period from 1991 to 2020,the proportion of high value areas(CSp>35,monthly C>10)has decreased to varying degrees,while the proportion of low value areas(CSp≤10,monthly C≤4)has *** trends of seasonal and monthly C in 82.2%~87.7%of the

关键词： Wind erosion climate erosivity Spatiotemporal 1characteristics Climate change Rebounded trend Typical black soil region Northeast China

The microtubule aster formation and its role in nuclear envelope assembly around the sperm chromatin in Xenopus egg extracts

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Chinese Science Bulletin 2003年第18期48卷 1912-1918页

作者： YANG Ning CHEN Zhongcai lu ping ZHANG Chuanmao zhai zhonghe TANG Xiaowei Institute of High Energy Physics Chinese Accademy of SciencesBeij ing 100039China Department of Cell Biology and Genetics Peking UniversityBeijing 100871China National Laboratory of Biomembrane and Membrane Biotechnology College of Life SciencesPeking UniversityBeijing 100871China Department of Physics Zhejiang UniversityHangzhou 310027China

Nuclear envelope is a dynamic structure in the cell cycle. At the beginning of mitosis, nuclear envelope breaks down and its components disperse into the cytoplasm. At the end of mitosis, nuclear envelope reassembles using the dispersed components. Searching for the mechanisms of the nuclear disassembly and reassembly has for a long time been one of the key projects for cell biologists. In this report we show that microtubules take a role in the nuclear envelope assembly around the sperm chromatin in Xenopus egg ex-tracts. Microtubule cytoskeleton has been demonstrated to take roles in the transport of intracellular membranes such as Golgi and ER vesicles. We found that the nuclear envelope assembly needs functional microtubules. At the beginning of the nuclear assembly, microtubules nucleated to form a microtubule aster around the centrosome at the base of the sperm head. Using the microtubule drug colchicine to dis-rupt the microtubule nucleation, nuclear envelope reassem-bly was seriously inhibited. If the microtubules were stabi-lized by taxol, another microtubule drug, the nuclear enve-lope reassembly was also interfered, although a significantly large aster formed around the chromatin. Based on these observations, we propose that microtubules play an impor-tant role in the nuclear envelope reassembly maybe by transporting the nuclear envelope precursors to the chroma-tin surfaces.

关键词： nuclear reassembly microtubule colchicine taxol Xenopus egg extracts.

Procyanidins Inhibit Tumor Angiogenesis by Crosslinking Extracellular Matrix

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Chinese Journal of Cancer Research 2011年第2期23卷 99-106页

作者： Wan-yin zhai Chun-ping Jia hui Zhao Yuan-sen Xu State Key Lab of Transducer Technology Shanghai Institute of Microsystem and Information Technology Chinese Academy of Sciences Shanghai 200068 China Graduation School Chinese Academy of Sciences Beijing China Biomaterials and Tissue Engineering Research Center Shanghai Institute of Ceramics Chinese Academy of Sciences Shanghai China

Objective： Procyanidins （PC） are widely available natural polyphenols. The present study is designed to investigate if PC can inhibit angiogenesis in lung adenocarcinoma xenografts through crosslinking vascular extracellular matrix （ECM） and preventing proteolysis by matrix metalloproteinases （MMPs）. Methods： Using the in vitro MMP-2 proteolysis and in vivo subcutaneous implantation models, we investigated if PC crosslinking inhibits MMP-mediated proteolysis. Using a cultured cell detachment assay, an in vitro angiogenesis assay, and a cell proliferation assay, we investigated if PC inhibits MMP-2-mediated endothelial cell detachment, angiogenesis, and cell proliferation, respectively. Using tumor xenografts, we evaluated if PC can inhibit growth of lung adenocarcinoma. Results： PC crosslink vascular ECM proteins, protecting them against proteolysis by MMPs in vitro and in vivo, protecting cultured human umbilical vein endothelial cells from detachment by MMP-2, and inhibiting in vitro angiogenesis. However, PC （0.75-100 μg/mL） did not inhibit vascular and tumor cells proliferation. PC injections （30 mg PC/kg bodyweight） in situ had anticancer effects on xenografts of lung adenocarcinoma, most likely by inhibiting angiogenesis during ECM proteolysis by MMPs. Conclusion： The results suggest that PC may be important MMP inhibitors that can be used as therapeutic anticancer agents.

关键词： Procyanidins Crosslinking Extracellular matrix Matrix metalloproteinases Angiogenesis

Grape Seed Procyanidin Extract Attenuate Sodium Fluoride-induced Oxidative Damage and Apoptosis in Rat Kidneys

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Biomedical and Environmental sciences 2020年第6期33卷 454-457页

作者： WANG Er hui YU Zeng Li ping Guan Fang zhai De Sheng Department of Nutrition and Food Hygiene College of Public HealthXinxiang Medical UniversityXinxiang 453003HenanChina Department of Pharmacy The First Affiliated HospitalXinxiang Medical UniversityWeihui 453100HenanChina

Fluoride is widely distributed in nature,and it can lead to fluorosis if intake is *** addition to its established effects on the skeleton and teeth,fluoride can also exert toxic effects on the *** can induce abnormal renal function in humans and other animals[1-3],but the mechanisms through which fluoride exerts such effects remain obscure.

关键词： fluoride skeleton intake

Targeted Search for Fast Radio Bursts with Nanshan 26m Radio Telescope

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Research in Astronomy and Astrophysics 2022年第6期22卷 64-71页

作者： Jian-Wei Mao Jian-ping Yuan Zhi-Gang Wen Jian Li Na Wang Pei Wang Rai Yuen Yu-Bin Wang Nan-Nan zhai Zhi-Yong Liu Mao-zheng Chen Guang-hui Li Xinjiang Astronomical Observatory Chinese Academy of SciencesXinjiang 830011China University of Chinese Academy of sciences Beijing 100049China National Astronomical Observatories Chinese Academy of SciencesBeijing 100101China

Fast radio bursts(FRBs)are radio transients that are bright and have short duration,with their physical mechanism not being fully *** conducted a targeted search for bursts from FRB 20201124A between 2021 June 2and July *** time-resolution data were collected for 104.5 hr using the ROACH2-based digital *** introduce the details of our FRB search pipeline which is based on HEIMDALL and *** of the injected mock FRBs search could help us better understand the performance of the pipelines,and improve the search algorithms and *** study the efficiency of our pipeline,5000 mock FRBs were injected into the data and searched using the *** results of the mock FRB search show that our pipeline can recover almost all(?90%)the injected mock FRBs above a signal-to-noise ratio(S/N)threshold of 15,and the performance is still acceptable(?80%)for injected S/Ns from 10 to *** recovery fraction displays relations with S/N,dispersion measure and pulse *** bursts were detected from FRB 20201124A in the middle of *** non-detection of FRB 20201124A may be due to its quiet phase window or no emission above the threshold of the Nanshan telescope.

关键词： transients fast radio bursts-methods observational-methods data analysis

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木瓜红枣复合饮料的工艺研究

食品科技 2010年第2期35卷 81-83页

作者：李慧芸翟文俊王汉屏陕西教育学院生命科学系西安710061 陕西教育学院生物工程研究所西安710061

以木瓜和红枣为原料,制成复合饮料。结果表明:木瓜红枣复合饮料的最佳工艺配方为复合果汁50%,v(木瓜汁)∶v(枣汁)=3∶2,白砂糖12%,柠檬酸0.15%;最佳稳定剂为明胶0.04%,CMC-Na0.10%,黄原胶0.08%;最佳杀菌条件为温度90℃、时间10min。

关键词：光皮木瓜红枣复合饮料

不同氮素水平下CO_2倍增对转Bt棉花氮素代谢的影响

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生态学杂志 2009年第11期28卷 2213-2219页

作者：高慧璟肖能文李俊生陈小云陈法军翟保平南京农业大学资源与环境学院南京210095 中国环境科学研究院环境科学研究所北京100012 南京农业大学植保学院昆虫学系南京210095

通过开顶式CO2气室研究了盛蕾期转Bt棉花新棉33B及其对照亲本DP5415的生长势和氮素代谢特征对土壤氮素水平(100和***-1)和CO2浓度倍增(750和375μl.L-1)的生理生态响应。结果表明:CO2浓度升高可显著提高2种棉花的株高和茎粗,增加生物产... 详细信息

通过开顶式CO2气室研究了盛蕾期转Bt棉花新棉33B及其对照亲本DP5415的生长势和氮素代谢特征对土壤氮素水平(100和***-1)和CO2浓度倍增(750和375μl.L-1)的生理生态响应。结果表明:CO2浓度升高可显著提高2种棉花的株高和茎粗,增加生物产量;氮素水平提高可显著增加转Bt棉花的株高、茎粗,以及茎和蕾的鲜质量,而对亲本棉花DP5415的影响不显著;对照棉花DP5415的谷氨酰胺合成酶(GS)活力随大气CO2浓度的升高而显著降低,随氮素营养的提高而升高,转Bt棉花新棉33B在低氮条件下,GS活力随大气CO2浓度的升高而显著增加;大气CO2浓度升高及氮素营养的增加使盛蕾期转Bt棉花的硝酸还原酶(NR)活力显著增加,DP5415的NR活力也随大气CO2浓度的升高而显著提高;大气CO2浓度对2种棉花的亚硝酸还原酶(NiR)活力都有明显的抑制作用,其中,高CO2浓度条件下,DP5415的NiR活力还随氮素营养的增加而显著下降。可见,大气CO2浓度升高下,土壤氮素水平变化对转Bt棉花的生长势影响显著,但对其氮素代谢生理的影响较对照亲本棉花小。生产中(尤其是高浓度CO2环境下),应进一步加强转Bt棉花的氮肥优化管理。

关键词：转Bt基因棉花大气CO2浓度倍增氮肥水平氮素代谢

Inhibition of KLF7-Targeting Micro RNA 146b Promotes Sciatic Nerve Regeneration

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Neuroscience Bulletin 2018年第3期34卷 419-437页

作者： Wen-Yuan Li Wei-Ting Zhang Yong-Xia Cheng Yan-Cui Liu Feng-Guo zhai ping Sun hui-Ting Li Ling-Xiao Deng Xiao-Feng Zhu Ying Wang Department of Anatomy Mudanjiang College of Medicine The Affiliated Hongqi Hospital Mudanjiang College of Medicine Department of Pathology Mudanjiang College of Medicine Department of Pharmacy Mudanjiang College of Medicine Spinal Cord and Brain Injury Research Group Stark Neuro-sciences Research Institute Indiana University School of Medicine

A previous study has indicated that Krüppel-like factor 7（KLF7）, a transcription factor that stimulates Schwann cell（SC） proliferation and axonal regeneration after peripheral nerve injury, is a promising therapeutic transcription factor in nerve injury. We aimed to identify whether inhibition of micro RNA-146 b（mi R-146 b）affected SC proliferation, migration, and myelinated axon regeneration following sciatic nerve injury by regulating its direct target KLF7. SCs were transfected with mi RNA lentivirus, mi RNA inhibitor lentivirus, or KLF7 si RNA lentivirus in vitro. The expression of mi R146 b and KLF7,as well as SC proliferation and migration, were subsequently evaluated. In vivo, an acellular nerve allograft（ANA） followed by injection of GFP control vector or a lentiviral vector encoding an mi R-146 b inhibitor was used to assess the repair potential in a model of sciatic nerve gap. mi R-146 b directly targeted KLF7 by binding to the 30-UTR, suppressing KLF7. Up-regulation of mi R-146 b and KLF7 knockdown significantly reduced the proliferation and migration of SCs, whereas silencing mi R-146 b resulted in increased proliferation and migration. KLF7 protein was localized in SCs in which mi R-146 b was expressed in ***, 4 weeks after the ANA, anti-mi R-146 b increased KLF7 and its target gene nerve growth factor cascade, promoting axonal outgrowth. Closer analysis revealed improved nerve conduction and sciatic function index score, and enhanced expression of neurofilaments, P0（anti-peripheral myelin）, and myelinated axon regeneration. Our findings provide new insight into the regulation of KLF7 by mi R-146 b during peripheral nerve regeneration and suggest a potential therapeutic strategy for peripheral nerve injury.

关键词： miR-146b KLF7 Schwann cells Sciatic nerve injury