In vitro reassembly of nuclear envelopes and organelles in Xenopus egg extracts
In vitro reassembly of nuclear envelopes and organelles in Xenopus egg extracts作者机构：College of Life Sciences Peking University Beijing China Institute of Molecular Medicine Peking University Beijing China
出 版 物：《Cell Research》 (细胞研究(英文版))
年 卷 期：2006年第16卷第7期
页 面：632-640页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种]
主 题：cell-free system endoplasmic reticulum Golgi apparatus mitochondria nuclear reconstitution Xenopus egg extracts
摘 要：We reconstituted bilayer nuclear membranes,multilayer membranes,and organelles from mixtures of Xenopus laevis eggextracts and demembranated Xenopus sperm *** proportions of the cytosolic and vesicular fractions from theeggs were used in the reconstitution mixtures.A cytosol:vesicle ratio of 10:1 promoted reassembly of the normal bilayernuclear membrane with inserted nuclear pore complexes around the decondensed Xenopus sperm chromatin.A cytosol:vesicle ratio of 5:1 caused decondensed and dispersed sperm chromatin to be either surrounded by or divided by unusualmultilayer membrane structures with inlaid pore complexes.A cytosol:vesicle ratio of 2.5:1 promoted reconstitution ofmitochondria,endoplasmic reticulum networks,and Golgi *** reassembly of the endoplasmic reticulumand Golgi apparatus,vesicular fragments of the corresponding organelles fused together and changed their shape to formflattened cisternae,which were then stacked one on top of another.
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