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Aframomum melegueta prevents the ejaculatory complications of propylthiouracil-induced hypothyroidism in sexually experienced male rats: Evidence from intravaginal and fictive ejaculations

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Journal of Integrative Medicine 2019年第5期17卷 359-365页

作者： Francois Xavier Kemka Nguimatio Patrick Brice Deeh Defo Modeste Wankeu-Nya Esther Ngadjui Albert Kamanyi pierre kamtchouing pierre Watcho Animal Physiology and Phytopharmacology Laboratory University of DschangP.O. Box 67DschangCameroon Department of Animal Organisms Biology University of DoualaP.O. Box24157 DoualaCameroon Department of Animal Biology and Physiology University of Yaounde 1P.O. Box 812YaoundeCameroon

Objective: Hypothyroidism has been claimed to generate sexual dysfunctions such as ejaculatory disorders. Aframomum melegueta is an aphrodisiac plant with pro-ejaculatory properties. We investigated the protective effects of aqueous extract(AE) and methanolic extract(ME) of A. melegueta on the ejaculatory function of hypothyroid male rats.Methods: Forty sexually experienced male rats were partitioned into 8 groups(5 rats per group) and treated for 28 d as follows: Group 1, Control;Group 2, propylthiouracil(PTU, 10 mg/kg)+ distilled water(DW,10 m L/kg);Group 3, PTU + 5% Tween 80(10 m L/kg);Group 4, PTU + bromocriptine(6 mg/kg);Group 5,PTU + AE(20 mg/kg);Group 6, PTU + AE(100 mg/kg);Group 7, PTU + ME(20 mg/kg), and Group 8,PTU + ME(100 mg/kg). On days 0, 7, 14 and 28 of treatment, each male rat was paired with primed receptive female for measurement of ejaculatory latency time(ELT) and post-ejaculatory interval(PEI) for1.5 h. On day 29, each male rat was urethane-anesthetized and the spinal cord was transected.Thereafter, following urethral/penile stimulations and intravenous injection of dopamine, contractions of the bulbospongiosus muscles and the intraseminal pressure were registered. After these recordings,blood was collected through the catheterization of abdominal artery and plasma was used for thyroidstimulating hormone(TSH), prolactin and testosterone assays.Results: PTU-induced hypothyroidism was characterized by a significant elevation(P < 0.001) of plasmatic TSH and prolactin levels, but a decline(P < 0.001) in plasmatic testosterone, compared to untreated group. ELT, PEI, contractions of the bulbospongiosus muscles and the intraseminal pressure were also altered by PTU treatment. On the contrary, A. melegueta extracts elevated testosterone(AE, 100 mg/kg,P < 0.01;ME, 100 mg/kg, P < 0.05) and decreased prolactin(AE, 100 mg/kg, P < 0.05;ME, 20 mg/kg,P < 0.05) levels, compared to corresponding controls. With regard to DW + PTU group, prolactin concentration was lowered(P < 0.05) in rats administered with br

关键词： Propylthiouracil Hypothyroidism Ejaculation Bromocriptine Aframomum melegueta

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Cyclophosphamide-induced reproductive toxicity: Beneficial effects of Helichrysum odoratissimum(Asteraceae) in male Wistar rats

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Journal of Integrative Medicine 2019年第5期17卷 366-373页

作者： pierre Watcho Ismaelle Rosine Mpeck Patrick Brice Deeh Defo Modeste Wankeu-Nya Esther Ngadjui Georges Romeo Bonsou Fozin pierre kamtchouing Albert Kamanyi Animal Physiology and Phytopharmacology Laboratory University of DschangP.O. BOX 67DschangCameroon Department of Animal Organisms Biology University of DoualaP.O. BOX 24157DoualaCameroon Department of Animal Biology and Physiology University of YaoundeP.O. BOX 812YaoundeCameroon

Objective: Cyclophosphamide(CP) is commonly used to treat some cancers, but its clinical efficacy is also linked with testicular toxicity. We investigated the effects of aqueous extract(AE) and methanolic extract(ME) of Helichrysum odoratissimum for reducing CP-induced reproductive toxicity in male rats.Methods: In addition to a normal control(group 1), drugs or vehicles were administered orally to seven groups(n = 5) of rats that had already received 4-weeks of pre-treatment with CP(5 mg/[kgád], per oral administration);group 2 received CP + distilled water(10 m L/[kgád]);group 3 received CP + 5% tween 80(10 m L/[kgád]);group 4 received CP + clomiphene citrate(0.25 mg/[kgád]);groups 5 and 6 received CP + AE(50 and 100 mg/[kgád]) and groups 7 and 8 received CP + ME(50 and 100 mg/[kgád]). Animals were sacrificed on day 15, and body and sexual organ weights, sperm characteristics, testosterone level and testicular histology were evaluated.Results: The CP-treated group showed a significant reduction(P < 0.001) in the body and seminal vesicle weights, testosterone level, sperm count, sperm motility and sperm viability, but elevated(P < 0.001)sperm morphological abnormalities and testicular structure alterations, compared to the control group.Interestingly, these detrimental effects of CP were reversed by treatment with H. odoratissimum extracts.For instance, both extracts and all doses of H. odoratissimum significantly increased the sperm count(P < 0.001), sperm motility(AE, 50 mg/kg, P < 0.05;ME, 50 and 100 mg/kg, P < 0.05) and sperm viability(AE, 50 mg/kg, P < 0.001;ME, 50 and 100 mg/kg, P < 0.001) compared to the CP group. H. odoratissimum also improved plasmatic and intratesticular testosterone levels and prevented histological alterations of the testes.Conclusion: H. odoratissimum might be considered as an alternative drug to alleviate/prevent reproductive damage in cancer patients receiving CP chemotherapy.

关键词： Helichrysum odoratissimum Cyclophosphamide Reproductive toxicity Rat

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Effects of extracts from Hibiscus macranthus and Basella alba mixture on testosterone production in vitro in adult rat testes slices

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Asian Journal of Andrology 2006年第1期8卷 111-114页

作者： Paul E Moundipa Silvère Ngouela pierre kamtchouing Etienne Tsamo Félicité M.Tchouanguep Serge Carreau Laboratory of Nutrition and Biochemical Toxicology Laboratory of Organic Chemistry Department of Animal Biology and Physiology University of Yaoundé IP.O.Box 812YaoundéCameroon Faculty of Science University of DschangP.O.Box 67DschangCameroon USC INRA-EA 2608.Laboratoire de Biochimie Esplanade de la paixUniversité de Caen14032 Caen-cedexFrance

Aim： To find an in vitro system for the measurement of the androgenic effects of different extracts of Hibiscus macranthus （Malvaceae） and Basella alba （Basellaceae）. Methods： The production of testosterone from testes slices incubated in two media, either Krebs-Henseleit buffer containing 0.5% Bovine serum albumin （BSA） or Dubecco＇s Modified Eagle＇s medium-F12 Ham nutrient mixture （DME/Ham F12）, under a mixture of 5% CO2 in 95% air was determined either in the presence or absence of cofactors and Hibiscus macranthus plus Basella alba （HMBA） extracts. Results： The testosterone production was increased in testes slices incubated in DME/Ham F12 medium in response to the cofactors （49%） and aqueous extracts （34%-60% according to dilutions）. Under the same atmospheric conditions, there was no positive response of the testes slices to either cofactor or HMBA extract stimulation in Krebs-Henseleit buffer containing 0.5% BSA, In further investigations related to the effect of HMBA, the DME/ Ham F12 medium was used. The results obtained from the in vitro test showed that the activity was present mainly in methylene chloride and methanol, since these extracts induced an increase in testosterone production by testes slices. Conclusion： The testes slice system is suitable to be used for further in vitro investigations of the isolation of androgenic bioactive components of plants. （Asian J Androl 2006 Jan; 8： 111-114）

关键词： testis testosterone plant extracts Hibiscus macranthus Basella alba

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Androgenic effect of Mondia whitei roots in male rats

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Asian Journal of Andrology 2004年第3期6卷 269-272页

作者： pierre Watcho pierre kamtchouing Selestin D.Sokeng Paul F.Moundipa Justine Tantchou Jean L.Essame Noussithe Koueta Animal Physiology and Phytopharmacology Laboratory Faculty of ScienceUniversity of DschangDschangCameroonAnimal Physiology LaboratoryUniversity of Yaounde IYaoundeCameroonDepartment of BiochemistryFaculty of SciencesUniversity of Yaounde IYaoundeCameroonDepartment of Animal Biology Faculty of Science University of Ngaoundere Ngaoundere CameroonUniversity Hospital CentreYaoundeCameroonUniversity Hospital CentreYaoundeCameroonAnimal Physiology LaboratoryUniversity of Yaounde IYaoundeCameroon

Aim: To determine the effect of the aqueous extract of Mondia whitei (Periplocaceae) roots on testosterone production and fertility of male rats. Methods: Adult male Wistar rats were used. In the acute study, 20 rats were randomly divided into 5 groups of 4 animals each. Four treated groups were administered orally a single dose of Mondia whitei (400 mg/kg) and the controls received a similar amount of distilled water. One group of animals were sacrificed by cervical dislocation 1,2,4 and 6 h after treatment, respectively. The controls were sacrificed at 6 h. Testicular testosterone was determined by radioimmunoassay. In the chronic study, 28 rats were divided at random into 4 groups of 7 animals each: Groups 1, 2 and 3 were given orally the plant extract (400 mg·kg-1·day-1) for 2, 4 and 8 days, respectively. The animals of Groups 1 and 2 were sacrificed 24 hours after the last dosing. The controls (Group 4) received the same amount of distilled water for 8 days. The fertility was assessed only in Groups 3 and 4 and after that, the animals were sacrificed and the epididymal sperm density, the serum testosterone and the testicular testosterone and 17β-estradiol were assayed. The serum, testicular and epidydimal protein contents were also determined. Results: In the acute treatment groups, the serum and testicular concentrations of testosterone remained unchanged at all the time points. Chronic treatment for 8 days induced a significant increase in the testicular weight, the serum and testicular testosterone, the testicular protein content and the sperm density (P<0.05-0.01), but did not affect the accessory gland weights, the serum protein contents, the testicular concentration of 17β-estradiol and the fertility compared to the controls. Conclusion: Mondia whitei root extract possesses an androgenic property.

关键词： Mondia whitei testosterone sperm density fertility

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The methanolic extract ofGuibourtia tessmannii(Caesalpiniaceae)improves sexual parameters in high fat diet-induced obese sexually sluggish rats

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Asian pacific Journal of Reproduction 2017年第5期6卷 202-211页

作者： Patrick Brice Deeh Defo Modeste Wankeu-Nya Esther Ngadjui Georges Romeo Bonsou Fozin Francois Xavier Kemka Albert Kamanyi pierre kamtchouing pierre Watcho Animal Physiology and Phytopharmacology Laboratory University of DschangBox 67 DschangCameroon Department of Animal Organisms Biology University of Douala-Cameroon Department of Animal Biology and Physiology University of Yaoundé1YaoundéCameroon

Objective:To evaluate the effects of the methanolic extract ofGuibourtia tessmannii (G. tesmannii) on sperm parameters, lipid profile and testosterone level in obese rats.Methods: A total of 193 male Wistar rats were fed either with palm oil diet (n=185) or standard diet (n=8) for 16 wk. At the end of this feeding period, 90 obese rats were selected and randomly divided into 18 groups of five rats each and treated with distilled water (10 mL/kg), vitamin E (75 mg/kg), clomiphene citrate (2 mg/kg) or methanolic extract ofG. tessmannii (55, 110 or 220 mg/kg) for 7, 21 or 56 d. At the end of each treatment period, sperm parameters, lipid profile and testosterone level were evaluated. Data were analyzed using ANOVA for repeated measures followed by post-hoc Tukey HSD (P<0.05) for multiple comparisons.Results: Feeding of rats for 16 wk with palm oil diet significantly damaged sperm parameters. The methanolic extract ofG. tesmannii improved sperm viability, motility and normality after 21 or 56 d of treatment. The sperm normality increased significantly in rats treated with the methanolic extract ofG. tesmannii for 7 (110 mg/kg,P<0.01) and 56 d (110 and 220 mg/kg,P<0.05) compared to control group. Triglycerids, total cholesterol, low and very low density lipoproteins cholesterol levels were lower in rats treated with the plant extract for 56 d.G. tesmannii also significantly increased the high density lipoprotein cholesterol and testosterone levels in the plasma after 56 d of treatment.Conclusions: The methanolic extract ofG. tesmannii can improve sperm parameters, lipid profile and testosterone level in obese rats. These findings may justify the folkloric use ofG. tesmannii as a reproductive performance enhancer.

关键词： Guibourtia tessmannii Palm oil diet Sperm parameters Lipid profile Testosterone

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The antihyperglycemic effect of Bridelia ndellensis ethanol extract and its fractions is mediated by an insulinotropic action

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Journal of Diabetes Mellitus 2013年第3期3卷 111-115页

作者： Selestin Dongmo Sokeng Begum Rokeya Jamadder Mohammed Abdul Hannan Liaquat Ali pierre kamtchouing Department of Biological Sciences University of Ngaoundéré Ngaoundéré Cameroon Department of Pharmacy North South University Dhaka Bangladesh Laboratory of Animal Physiology Faculty of Science University of Yaounde Yaounde Cameroon Research Division Bangladesh Institute of Research and Rehabilitation in Diabetes Endocrine and Metabolic Disorders (BIRDEM) Dhaka Bangladesh

Backgroung: Bridelia ndellensis (Euphorbiaceae) is used as a traditional plant treatment of diabetes. The aim of the present study was to investigate the mechanism involved in the antihyperglycemic effects of this plant. Methods: An intestinal perfusion technique was used to study the effect of the ethanol extract of B. ndellensis on the intestinal absorption of glucose in normal rats fasted for 36 h. The effect of the extract (50 and 100 mg/kg) on Insulin secretion was evaluated using BRIN-BD11 pancreatic cells. Results: In a perfused rat intestinal preparation in situ, the plant extract at a concentration of 5 mg/ml did not show any inhibitory effects on glucose absorption when compared with control animals. Ouabain which is an inhibitor of glucose transport across the small intestine significantly (p < 0.001) decreased the glucose absorption during the whole perfusion period compared with the control group. On the other hand, the plant extract inhibited the gastrointestinal motility induced by Barium sulphate milk in rats. The ethanol extract of B. ndellensis at concentrations of 50 and100mg/ml stimulated in a concentration-dependent manner the insulin secretion from BRIN-BD11 cells in vitro. The induction of insulin release was significantly different when compared to control with the concentration of 100 μg/ml (P < 0.001). Alanine (10 mM) used as positive control, significantly (p < 0.001) stimulated insulin release from BRIN-BD11 cells. Conclusion: The present study has revealed that B. ndellensis ethanol extract stimulates insulin secretion and does not affect the glucose absorption, and it has suggested that the antihyperglycemic effects of B. ndellensis is partly mediated by the stimulation of insulin secretion.

关键词： Bridelia ndellensis Ethanol Extract Intestinal Glucose Absorption Insulin Secretion in Vitro

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Evaluation of androgenic activity of Zingiber officinale and Pentadiplandra brazzeana in male rats

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Asian Journal of Andrology 2002年第4期4卷 299-301页

作者： pierrekamtchouing GisèleYolandeMbongueFandio ThéophileDimo Hermine Boukeng Jatsa Laboratoire de Physiologie Animale Faculté des Sciences Université de Yaoundé I B.P.812 Yaoundé CamerounLaboratoire de Physiologie Animale Faculté des Sciences Université de Yaoundé I B.P.812 Yaoundé CamerounLaboratoire de Physiologie Animale Faculté des Sciences Université de Yaoundé I B.P.812 Yaoundé CamerounDépartement de Biologie et de Physiologie Animales Faculté des Sciences Université de Douala B.P. 24157 Douala Cameroun

Aim: Aqueous extracts of Zingiber officinale and Pentadiplandra brazzeana were tested for their possible androgenic activity in male Wistar rats. Methods: The aqueous extracts of the two plants were gavaged separately to 2 groups of rats at a similar dose of 600 mg.kg^1.day-1 for 8 days. At the end of the treatment, the animals were killed and the blood, testis, epididymis, seminal vesicles and prostate were collected for biochemical analysis. Results: The aqueous extract of Z. officinale significantly increased in the relative weight of the testis, the serum testosterone level, testicular cholesterol level and epididymal α-glucosidase activity. The aqueous extract of P. brazzeana significantly increased the weights of the testis, seminal vesicles and prostate. It also significantly increased the serum and testicular testosterone level. The fructose, α-glucosidase and cholesterol levels in P. brazzeanatreated rats were increased by 28%, 35% and 114%, respectively. Conclusion: The aqueous extracts of both P. brazzeana and Z. officinale have an androgenic activity, which seems to be more potent with P. brazzeana than with Z. officinale. (Asian J Androl 2002 Dec; 4: 299-301)

关键词： P. brazzeana Z. officinale testosterone androgenic activity

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In Vitro Evaluation of Sida pilosa Retz (Malvaceae) Aqueous Extract and Derived Fractions on Schistosoma mansoni

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Pharmacology & Pharmacy 2015年第8期6卷 380-390页

作者： Hermine Boukeng Jatsa Cintia Aparecida de Jesus Pereira Ana Barbara Dias Pereira Deborah Aparecida Negrao-Correa Fernao Castro Braga Glauber Meireles Maciel Rachel Oliviera Castilho pierre kamtchouing Mauro Martins Teixeira Laboratory of Animal Physiology Department of Animal Biology and PhysiologyFaculty of ScienceUniversity of YaoundeIYaoundeCameroon Laboratory of Schistosomiasis Department of ParasitologyInstitute of Biological SciencesFederal University of Minas GeraisBelo HorizonteBrazil Laboratory of Immunopharmacology Department of Biochemistry and ImmunologyInstitute of Biological SciencesFederal University of Minas GeraisBelo HorizonteBrazil Laboratory of Phytochemistry Department of Pharmaceutical ProductsFaculty of PharmacyFederalUniversity of Minas GeraisBelo HorizonteBrazil Laboratory of Pharmacognosy Department of Pharmaceutical ProductsFaculty of PharmacyFederalUniversity of Minas GeraisBelo HorizonteBrazil

Sida pilosa Retz. (Malvaceae) is a medicinal plant used in Africa for the treatment of dysmenorrhea, lower abdominal pains and intestinal helminthiasis. S. pilosa aqueous extract and derived fractions were investigated for their bioactivity against Schistosoma mansoni. The aqueous extract from S. pilosa aerial parts (1.25 - 40 mg/mL) and derived fractions (n-hexane, DCM, EtOAc and n-BuOH: 0.25 - 8 mg/mL) were tested on adult S. mansoni maintained in a GMEN culture medium. Praziquantel was used as the reference drug. After 24 h of incubation, worms were monitored for their viability and egg output. The antioxidant activity of S. pilosa was evaluated by the ability to scavenge the 2,2-diphenyl-1-picrylhydrazyl free radicals. The chemical composition of the n-BuOH fraction was investigated by HPLC-MS analysis. S. pilosa aqueous extract and fractions significantly increased worm mortality in a concentration-dependent manner. The n-BuOH fraction was the most active with a LC50 of 1.25 mg/mL. Significant reduction of motor activity (25% to 100%) was recorded for surviving worms incubated in different concentrations of the extract and fractions. Incubation of S. mansoni in different concentrations of S. pilosa extract and fractions led to significant reduction of egg laying (52% to 100%). The aqueous extract and derived fractions exhibited antioxidant activity in a concentration-dependent manner. The highest antioxidant activity was found with the EtOAc fraction, followed by the DCM and n-BuOH fractions. HPLC-MS analysis of the n-butanol fraction revealed the presence of two indoloquinoline alkaloids. This study disclosed the schistosomicidal activity of the n-butanol fraction from S. pilosa aqueous extract. This activity is probably related to the indoloquinoline alkaloids identified in the fraction.

关键词： Sida pilosa Schistosomicidal Activity Antioxidant Activity Indoloquinoline Alkaloids Schistosoma mansoni

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