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Implementation of a New Solution for the Preservation of Anatomical Specimens Made of Non-Toxic Substances

open Journal of Veterinary Medicine 2024年第3期14卷 56-67页

作者： Ma reyes pichardo-Molinero Samantha Jar.o.-Xicotencatl Misael r. oliver-González Car.o. G. García-Tovar Laborator.o.de Apoyo Técnico a la Anatomía Facultad de Estudios Superiores Cuautitlán Universidad Nacional Autónoma de México Estado de México Mexico Unidad de Investigación Multidisciplinaria L4 (Mor.o.ogía Veterinaria y Biología Celular) Facultad de Estudios Superiores Cuautitlán Universidad Nacional Autónoma de México Estado de México Mexico

The preservation of anatomical pieces in Veterinary Anatomy is essential since it is not possible to dissect all domestic species. Most techniques use reagents with high levels of toxicity such as formaldehyde. The objective of this work was to develop a new preservation technique that uses reagents with zero toxicity and that allows obtaining preserved pieces suitable for anatomical studies. The alcohol propylene glycol technique was developed, the method of which uses a fixation step with alcohol, sodium chloride, commercial vinegar and subsequently the impregnation of the preservation solution made from propylene glycol and commercial vinegar, which are non-toxic. As a result of this work, adequately preserved sheep hearts were obtained that preserved their morphology with slight changes in size and weight, which did not affect their external and internal anatomical structure. Its coloration was not substantially affected, remaining a little lighter. The pieces obtained showed flexibility which allowed dissections to be carried out. The time to develop the technique was 20 days. A comparative study was carried out with the phenolated glycerin technique that uses toxic reagents (formaldehyde and phenol) and the pieces obtained with the alcohol propylene glycol technique were of better quality, observing that the pieces with phenolated glycerin tend to darken and are more rigid. And the time to develop the technique is 24 days. In conclusion, a preservation technique for anatomical pieces was developed that allowed the preservation of the organs under study, which allow their use for anatomical studies, and which have been preserved without changes until the time of this publication (8 months) and there are pieces preserved with this technique for 2 years.

关键词： Veterinary Anatomy Heart Preservation Techniques Alcohol Propylene Glycol Technique Fixation Necropsy Toxic Chemicals

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皮肌炎自然缓解1例

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世界核心医学期刊文摘（皮肤病学分册） 2005年第1期1卷 52-52页

作者： Jordan J.r. Paruthi S. pichardo r.o. J.L.Jorizzo 崔荣 Center fo Dermatology research Department of DermatologyWake Forest Univ.School of MedicineMedicalCenter BoulevardWinston-SalemNC 27157-1071United States

Dermatomyositis (DM) is an inflammatory myopathy of skeletal muscle with characteristic cutaneous *** is a rare disorder with a bimodal age distribution that affects almost twice as many women as *** category of DM, normal-enzyme DM, is characterized by cutaneous changes only at baseline, normal serum muscle enzyme levels and myositis demonstrated by electromyography (EMG) and/or muscle biopsy ***, patients with normal-enzyme DM progress to severe muscle involvement and require systemic corticosteroid *** patient we report has normal-enzyme DM confirmed by serial serum enzymes, EMG, and skin and muscle biopsies but is unique in that she never experienced progression of muscle weakness although muscle involvement was documented histologically and by ***-up examination after 1 year revealed near-complete resolution of cutaneous involvement after topical therapy and no evidence of muscle weakness.

关键词：皮肌炎感染性肌病正常性肌肉活检血清肌酶肌无力女性发病率皮质类固醇年龄分布

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Phase equilibrium modelling of the amphibolite facies metamorphism in the Yelapa-Chimo Metamorphic Complex, Mexico

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Geoscience Frontiers 2021年第1期12卷 293-312页

作者： Fabian Gutierrez-Aguilar Peter Schaaf Gabriela Solís-pichardo Gerar.o.F.Arrieta-García Teodoro Hernandez-Trevino Car.o. Linares-Lopez Posgrado en Ciencias de la Tierra Instituto de GeofísicaUniversidad Nacional Autonoma de MexicoAv.Universidad 3000Ciudad de Mexico04510Mexico Laborator.o.Universitar.o.de Geoquímica Isotopica(LUGIS) Instituto de GeofísicaUniversidad Nacional Autonoma de MexicoAv.Universidad 3000Ciudad de Mexico04510Mexico Laborator.o.Universitar.o.de Geoquímica Isotopica(LUGIS) Instituto de GeologíaUniversidad Nacional Autonoma de MexicoAv.Universidad 3000Ciudad de Mexico04510Mexico Instituto de Geofísica Universidad Nacional Autonoma de MexicoAv.Universidad 3000Ciudad de Mexico04510Mexico

The Yelapa-Chimo Metamorphic Complex forms part of the Jalisco Block in western Mexico and exposes a wide range of Early Cretaceous metamorphic rocks;such as paragneiss,orthogneiss,amphibolites,and ***,the pressure-temperature(P-T)conditions of metamorphism and partial melting remain poorly studied in the *** elucidate metamorphic P-T conditions,phase equilibrium modelling was applied to two sillimanite-garnet paragneisses,one amphibole-orthogneiss,and one ***-garnet paragneisses exhibit a lepidoblastic texture with a biotite+sillimanite+kyanite+garnet+quartz+plagioclase+K-feldspar mineral ***-orthogneiss and amphibolite display a nematoblastic texture with an amphibole+(1)plagioclase+quartz+(1)titanite assemblage and an amphibole+(2)plagioclase+(2)titanite+ilmenite retrograde mineral *** calculated for the two sillimanite-garnet paragneiss samples show P-T peak conditions at~6-7.5 kbar and~725-740℃.The results for amphibole-orthogneiss and the amphibolite yield P-T peak conditions at~8.5-10 kbar and~690-710℃.The mode models imply that metasedimentary and metaigneous units can produce up to~20 vol%and~10 vol%of melt,*** within a closed system during isobaric heating suggests that melt compositions of metasedimentary and metaigneous units are likely to have direct implications for the petrogenesis of the Puer.o.Vallarta *** new data indicate that the Yelapa-Chimo Metamorphic Complex evolved through a metamorphic gradient between~23-33℃km^-1and the metamorphic rocks formed at depths between~22 km and~30 km with a burial rate of~2.0 km Ma^-***,the P-T data for both metasedimentary and metaigneous rocks provide new constraints on an accretionary framework,which is responsible for generating metamorphism and partial melting in the YelapaChimo Metamorphic Complex during the Early Cretaceous.

关键词： Phase equilibrium modelling Yelapa-Chimo Metamorphic Complex Amphibolite facies Partial melting

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Pronuclear formation by ICSI using chemically activated ovine oocytes and zona pellucida bound sperm

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Journal of Animal Science and Biotechnology 2017年第2期8卷 370-377页

作者： J.E.Hernández-pichardo Y.Ducolomb S.romo M.E.Kjelland r.Fier.o.F.Casillas M.Betancourt División de Ciencias Biológicas y de la Salud Departamento de Producción Agrícola y AnimalUniversidad Autónoma Metropolitana-XochimilcoCiudad de MexicoMexico Division de Ciencias Biologicas y de la Salud Departamento de Ciencias de la SaludUniversidad Autónoma Metropolitana-IztapalapaSan Rafael Atlixco 186CP 09340 Ciudad de MexicoMexico Departamento de Ciencias Pecuarias Facultad de EstudiosSuperiores CuautitlanUNAMCiudad de MexicoEstado de MexicoMexico Conservation Genetics&BiotechLLCValley CityNDUSA Division de Ciencias Biologicas y de la Salud Departamento de Biologia de la ReproduccionUniversidad Autonoma Metropolitana-lztapalapaCiudad de MexicoMexico Doctorado en Biologia Experimental Universidad Autonoma Metropolitana-lztapalapaCiudad de MexicoMexico

Background： In order to improve ICSI, appropiate sperm selection and oocyte activation is necessary. The objective of the present study was to determine the efficiency of fertilization using ICSI with chemically activated ovine oocytes and sperm selected by swim up（SU） or swim up ＋ zona pellucida（SU ＋ ZP） ***： Experiment 1, 4–20 replicates with total 821 in vitro matured oocytes were chemically activated with ethanol, calcium ionophore or ionomycin, to determine oocyte activation（precense of one PN）. Treatments showed similar results（54, 47, 42 %, respectively） but statistically differents（P 0.05）.Conclusions： Chemical activation induces higher.o.ine oocyte activation than mechanical activation. Ethanol slightly displays higher.o.cyte activation than calcium ionophore and ionomicine. Sperm selection with SU ＋ ZP increased Ar/A and Ar/D rates in comparison with SU in fresh and frozen-thawed sperm. According to this, in terms of fertilization rates, chemical activation after ICSI increased oocyte PN formation compared to mechanical activation. Also, fresh sperm treated with SU and SU ＋ ZP were significantly different than frozen-thawed sperm,but between sperm treatments no significant differences were obtained.

关键词： Ethanol ICSI oocyte activation ovine Pronucleus Zona pellucida

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西班牙塞维利亚市男性同性恋患者中的性传播疾病

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地方病译丛 1992年第2期13卷 50-51页

作者： A r pichardo 牛新玲

过去10年人们越来越意识到,在有多个匿名性侣的男性同性恋者中患性传播疾病的危险增加,可能有症状,也可能无症状。在男性同性恋中发现的性病与异性恋者的类型有所不同。由于在西班牙除淋病和梅毒外均缺乏可靠的统计,我们进行了前瞻性的... 详细信息

过去10年人们越来越意识到,在有多个匿名性侣的男性同性恋者中患性传播疾病的危险增加,可能有症状,也可能无症状。在男性同性恋中发现的性病与异性恋者的类型有所不同。由于在西班牙除淋病和梅毒外均缺乏可靠的统计,我们进行了前瞻性的研究,以便确定两组到塞维利亚医学院性病诊所就诊的男性同性恋者的性病流行情况。该市人口为

关键词：男性同性恋异性恋同性恋者性病性传播疾病传染病淋球菌尿道炎患者塞维利亚西班牙南欧

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raman spectroscopy for detection of imatinib in plasma: A pr.o. of concept

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Journal of Innovative optical Health Sciences 2015年第5期8卷 56-66页

作者： Sanhita rath Aditi Sahu Vikram Gota P.G.Martínez-Torres J.L.pichardo-Molina C.Murali Krishna Department of Clinical Pharmacology ACTREC Tata Memorial CenterKharghar Navi Mumbai 410210 India Chilakapati Lab ACTRECTata Memorial Center KhargharNavi Mumbai 410210 India Instituto de Fisica y Matematicas Universidad Michoacana de San Nicolas de Hidalgo Edificio C-3 Ciudad Universitaria CP.58040 MoreliaMichoacanMexico Centro de Investigaciones enoptica AC C.P.37#150 Leon Gto.Mexico

Imatinib is the standard first line treatment for chronic myeloid leukemia(CML).owing to doserelated toxicities of Imatinib such as neutropenia,there is scope for treatment optimization through therapeutic drug monitoring(TDM).Trough concentration of 1g/mL is considered the therapeutic *** methods for the detection of Imatinib in plasma are limited by long read out time and expensive ***,raman spectroscopy was explored as a rapid and objective tool for monitoring Imatinib *** approaches:conventional raman spectroscopy(CrS),Drop coating deposition raman(DCDr)spectroscopy and surface-enhanced raman spectroscopy(SErS)were employed to detect the required trough concentration of 1g/mL and *** of therapeutically relevant concentrations(1g/mL)using SErS and suitable nanoparticle substrates has been ***,rigorous validation using clinical samples is necessary to confirm the utility of this approach in routine clinical usage.

关键词： Therapeutic drug monitoring raman spectroscopy Imatinib SErS DCDr chronic myeloid leukemia

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High Intensity Focused Ultrasound optimal Device Design for Targeted Prostate Cancer Treatment: A Numerical Study

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Journal of Modern Physics 2013年第2期4卷 240-245页

作者： Laura Curiel Dexter Hobson Jean-Yves Chapelon Samuel pichardo Lakehead University Thunder Bay Regional Research Institute Ontario Canada National Institute of Health and Medical research (INSErM) Lyon France

A parametric study was per.o.med to design a device capable of treating small targeted regions within the prostate using high intensity focused ultrasound, while sparing the sur.o.nding organs and minimizing the number.o. elements. The optimal focal length (L), operating frequency (f), element size (a) and central opening radius for lodging an imaging probe (r) of a device that would safely treat tissue within the prostate were obtained. Images from the Visible Human Project were used to determine simulated organ sizes and treatment locations. Elliptical tumors were placed throughout the simulated prostate and their lateral and axial limits were selected as test locations. Using graphics processors, the acoustic field and Bio-Heat Transfer Equation were solved to calculate the heating produced during a simulated treatment. L, f, a and r were varied from 45 to 75 mm, 2.25 to 3 MHz, 1.5 to 8 times the wavelength and 9 to 12.5 mm, respectively. The resulting optimal device was a 761-element concentric-ring transducer with L = 68 mm, f = 2.75 MHz, a = 2.05λ and r = 9 mm. Simulated thermal lesions showed that it was possible to treat target tumors consistent with reported locations and sizes for prostate cancer.

关键词： Focused Ultrasound Prostate Cancer Thermal Ablation Non-Invasive Treatment BHTE HIFU

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Low serum levels of ghrelin are associated with gallstone disease

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World Journal of Gastroenterology 2006年第19期12卷 3096-3100页

作者： Nahum Méndez-Sánchez Guadalupe Ponciano-rodríguez Luisa Bermejo-Martínez Antonio r Villa Norber.o.C Chávez-Tapia Daniel Zamora-Valdés raúl pichardo-Bahena Blanca Barredo-Prieto Martha H Uribe-ramos Martha H ramos Héctor A Baptista-González Misael Uribe Liver Unit Biomedical research Department Medica Sur Clinic and FoundationMexico CityMexico Faculty of Medicine National Autonomous University of Mexico (UNAM) Mexico CityMexico Clinical research Department National Institute of Perinatology Mexico CityMexico Clinical Epidemiology Unit National Institute of Medical Sciences and Nutrition Mexico CityMexico

AIM： To explore the role of ghrelin in gallstone disease. METHoDS： We carried out a cross-sectional study in 150 subjects, 38 with gallstones （cases） and 112 controls. We also did a real-time PCr-rT study in twenty gallbladder samples each. Body mass index （BNI）, serum insulin, ghrelin, and serum lipids were measured. Logistic regression analyses （univariate and multivariate） were conducted to estimate the probability of gallstone disease associated with serum ghrelin concentrations. rESULTS： Cases were statistically different from controls in gender distribution （P = 0.01）, age （53 vs 44 yr, P = 0.002）, BNI （28 vs 25; P = 0.004）, and glucose （5.26 vs 4.98 mmol/L; P = 0.05）. The prevalence of ghrelin serum levels above the third tercile was lower in subjects without metabolic syndrome （P 〈 0.05）. In a multivariate model, we found a protective effect, when ghrelin values were higher than the median value （or = 0.27, 95%CI 0.09-0.82, P = 0.02）. Twenty （20%） gallbladder specimens expressed ghrelin mrNA. CoNCLUSIoN： Serum ghrelin concentrations are associated with a protective effect of GD.

关键词： Gallstones obesity Ghrelin Gallbladder Gastrointestinal hor.o.es

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天生慈母心

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疯狂英语（阅读版） 2005年第10期 34-37页

作者： Winifred pichardo 柏柏

回首走过的生命历程,我不禁想问,我都做了些什么重要或有意义的事情,我取得了哪些方面的成就呢?我对身边的人,尤其是心爱的那些人,都产生了一些什么影响或者让他们收获到了什么呢?

关键词：生命历程成就收获意义母亲交流照顾重要方面新生活

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Effect of Different reproductive Stages and Culture Times on Domestic Cat <i>in Vitro </i>oocyte Maturation

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Advances in reproductive Sciences 2021年第2期9卷 129-138页

作者： José Ernesto Hernández pichardo Miguel ramses Del Moral reyes Michael E. Kjelland José Luis rodríguez Suastegui Department of Agricultural and Animal Production Division of Biological and Health SciencesMetropolitan Autonomous University-Xochimilco Mexico City Mexico Private Clinic Mexico City Mexico Conservation Genetics & Biotech LLC Valley City USA Biological and Health Sciences PhD Program Metropolitan Autonomous University-Xochimilco Mexico City Mexico

The domestic cat has been used as a model to carry out comparative research in assisted reproduction, to be applied in wild cats. The efficiency in domestic cat IVM concerning the reproductive status and/or cultivation times has previously been investigated;however, the studies were carried out separately. The objective of this research was to evaluate the maturation of oocytes of domestic cats of different reproductive stages using two different in vitro culture times. The ovaries were obtained by Ooforo-Salpingo-Hysterectomy of cats that were of the following groups: 1) prepubertal, 2) follicular, 3) pregnant or 4) in anestrus. Maturation was carried out with TCM199 medium supplemented with BSA for 24 h and 48 h. On average, 29 ± 25, 20 ± 15, 17 ± 9 and 17 ± 13 oocytes/cat were recovered from the prepubertal follicular, pregnant, and anestrus stages, respectively, but did not show a significant difference (P > 0.05). Also, meiotic maturation did not show a significant difference between the different reproductive stages at 24 h and 48 h, respectively (P > 0.05). However, in the prepubertal and follicular stages, greater oocyte maturation numbers were observed at 48 h compared to 24 h (P result was not observed in the pregnant and anestrus stages (P > 0.05), indicating that the in vitro culture duration is an important factor during in vitro maturation of domestic cat oocytes.

关键词： Domestic Cat oocytes In Vitro Maturation Culture Time Estrus Cycle Stages

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