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Mitotic motor CENP-E cooperates with PRC1 in temporal control of central spindle assembly

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Journal of Molecular Cell Biology 2020年第8期12卷 654-665页

作者： Xu Liu Leilei Xu Junying Li phil y.yao Wanjuan Wang Hazrat Ismail Haowei Wang Bryce Liao Zhihong yang Tarsha Ward Ke Ruan Jianchun Zhang Quan Wu Ping He Xia Ding Dongmei Wang Chuanhai Fu Zhen Dou Feng yan Wenwen Wang Xing Liu Xuebiao yao MOE Key Laboratory for Cellular Dynamics&Anhui Key Laboratory for Chemical Biology CAS Center for Excellence in Molecular Cell ScienceHefei National Science Center for Physical Sciences at Microscale&University of Science and Technology of ChinaHefei 230027China Department of Physiology Morehouse School of MedicineAtlantaGA 30310USA School of Basic Medical Sciences Beijing University of Chinese MedicineBeijing 100029China Department of Biology Duke University DurhamNC 27708USA Institute of ProteoGenomics Beijing 100029China Harvard Medical School BostonMA 02115USA

Error-free cell division depends on the accurate assembly of the spindle midzone from dynamic spindle microtubules to ensure chromatid segregation during metaphase-anaphase ***,the mechanism underlying the key transition from the mitotic spindle to central spindle before anaphase onset remains *** the prevalence of chromosome instability phenotype in gastric tumorigenesis,we developed a strategy to model context-dependent cell division using a combination of light sheet microscope and 3D gastric *** sheet microscopic image analyses of 3D organoids showed that CENP-E inhibited cells undergoing aberrant metaphase-anaphase transition and exhibiting chromosome segregation errors during *** real-time imaging analyses of 2D cell culture revealed that CENP-E inhibited cells undergoing central spindle splitting and chromosome instability *** biotinylated syntelin as an affinity matrix,we found that CENP-E forms a complex with PRC1 in mitotic *** inhibition of CENP-E in metaphase by syntelin prevented accurate central spindle assembly by perturbing temporal assembly of PRC1 to the ***,CENP-E-mediated PRC1 assembly to the central spindle constitutes a temporal switch to organize dynamic kinetochore microtubules into stable midzone *** findings reveal a previously uncharacterized role of CENP-E in temporal control of central spindle *** CENP-E is absent from yeast,we reasoned that metazoans evolved an elaborate central spindle organization machinery to ensure accurate sister chromatid segregation during anaphase and cytokinesis.

关键词： organoids.cell division central spindle CENP-E syntelin PRC1

Chromothripsis:an emerging crossroad from aberrant mitosis to therapeutic opportunities

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Journal of Molecular Cell Biology 2024年第4期16卷 1-16页

作者： Umer Ejaz Zhen Dou phil yyao Zhikai Wang Xing Liu Xuebiao yao MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics University of Science and Technology of China School of Life SciencesHefei 230027China Anhui Key Laboratory for Chemical Biology Hefei National Science Center for Inter-disciplinary SciencesHefei 230027China University of California San Diego School of Medicine San DiegoCA 92103USA

Shen Chromothripsis,a type of complex chromosomal rearrangement originally known as chromoanagenesis,has been a subject of extensive investigation due to its potential role in various diseases,particularly *** involves the rapid acquisition of tens to hundreds of structural rearrangements within a short period,leading to complex alterations in one or a few *** phenomenon is triggered by chromosome mis-segregation during *** in accurate chromosome segregation lead to formation of aberrant structural entities such as micronuclei or chromatin *** association between chromothripsis and cancer has attracted significant interest,with potential implications for tumorigenesis and disease *** review aims to explore the intricate mechanisms and consequences of chromothripsis,with a specific focus on its association with mitotic ***,we discuss a comprehensive analysis of crucial molecular entities and pathways,exploring the intricate roles of the CIP2A–TOPBP1 complex,micronuclei formation,chromatin bridge processing,DNA damage repair,and mitotic ***,the review will highlight recent advancements in identifying potential therapeutic targets and the underlying molecular mechanisms associated with chromothripsis,paving the way for future therapeutic interventions in various diseases.

关键词： genomic stability DNA damage repair chromothripsis synthetic lethality cell division

Effects of dietary ratio of n-6 to n-3 polyunsaturated fatty acids on immunoglobulins, cytokines, fatty acid composition, and performance of lactating sows and suckling piglets

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Journal of Animal Science and Biotechnology 2013年第2期4卷 137-144页

作者： Wei yao Jie Li Jun jun Wang Weiliang Zhou Qingbo Wang Rongchang Zhu Fenglai Wang phil Thacker Ministry of Agriculture Feed Industry Centre State Key Laboratory of Animal Nutrition China Agricultural University Tianjin National Breeding Pig Farm Ninghe Livestock & Fisheries Bureau Department of Science University of Saskatchewan

This experiment was conducted to investigate the effects of dietary ratios of n-6：n-3 polyunsaturated fatty acids （PUFA） on the performance of lactating sows and their piglets. Thirty pregnant Landrace sows were assigned to one of three treatments from d 108 of gestation until weaning （26-29 d） and were fed diets containing different ratios of n-6：n-3 PUFA including 3：1,9：1 and 13：1. The effects on sow and litter production traits were examined together with an assessment of sow body condition. No differences were detected among the treatments for the daily feed intake of sows or changes in sow weight and back-fat levels during lactation （P 〉 0.05）. Litter size at d 14 and d 21 were tended to increase in 3：1 treatment compared with 9：1 and 13：1 treatments （P 〈 0.10）. Litter weight gain （1.77 kg/d） from d 0 to d 14 was tended to increase in 9：1 groups compared with the other two treatments （P 〈 0.10）. A significant difference was observed for the content of a -linolenic acid, total n-3 PUFA, and the ratio of n-6：n-3 PUFA in the colostrum, milk, and piglets plasma （P 〈 0.01）. The effects of different ratios of n-6：n-3 PUFA in sow diets on colostrum, milk and piglet plasma immunoglobulin concentrations are studied. No difference was observed among treatments in the concentrations of IgM, and IgA in colostrum （P 〉 0.05）. A great significant difference for IgG concentration was observed among 3 group in colostrum. A great significant difference for IgA, and IgM （P〈 0.01） concentrations in piglet plasma at d14 and a significant difference for IgG（P 〈 0.05） was observed at d14. Furthermore, at d 21 of lactation, piglet plasma IgG and IgA concentration were greater in 3：1 compared with 13：1 group （P 〈 0.01）. In summary, the current study demonstrated that altering the ratio of n-6：n-3 PUFA in lactating sow diet had an effect on the immune component including immunoglobulin and cytokines, and it tended to increase the litter average daily gain and improve the immune status o

关键词： n-6:n-3 polyunsaturated fatty acids Performance Fatty acid composition Immunoglobulin Cytokines

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Organization of microtubule plus-end dynamics by phase separation in mitosis

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Journal of Molecular Cell Biology 2024年第2期16卷 24-35页

作者： Fengrui yang Mingrui Ding Xiaoyu Song Fang Chen Tongtong yang Chunyue Wang Chengcheng Hu Qing Hu yihan yao Shihao Du phil yyao Peng Xia Gregory Adams Jr Chuanhai Fu Shengqi Xiang Dan Liu Zhikai Wang Kai yuan Xing Liu MOE Key Laboratory for Membraneless Organelles&Cellular Dynamics Hefei National Research Center for Cross-disciplinary SciencesCenter for Advanced Interdisciplinary Science and Biomedicine of IHMDivision of Life Sciences and MedicineUniversity of Science&Technology of ChinaHefei 230027China Anhui Key Laboratory for Cellular Dynamics and Chemical Biology University of Science&Technology of ChinaHefei 230027China Keck Center for Organoids Plasticity Morehouse School of MedicineAtlantaGA 30310USA Hunan Key Laboratory of Molecular Precision Medicine Central South UniversityChangsha 410083China

In eukaryotes,microtubule polymers are essential for cellular plasticity and fate ***-binding(EB)proteins serve as scaffolds for orchestrating microtubule polymer dynamics and are essential for cellular dynamics and chromosome segregation in ***,we show that EB1 forms molecular condensates with TIP150 and MCAK through liquid–liquid phase separation to compartmentalize the kinetochore–microtubule plus-end machinery,ensuring accurate kinetochore–microtubule interactions during chromosome segregation in *** of EB1–TIP150 polymer formation by a competing peptide prevents phase separation of the EB1-mediated complex and chromosome alignment at the metaphase equator in both cultured cells and Drosophila ***220 of EB1 is dynamically acetylated by p300/CBP-associated factor in early mitosis,and persistent acetylation at Lys220 attenuates phase separation of the EB1-mediated complex,dissolves droplets in vitro,and harnesses accurate chromosome *** data suggest a novel framework for understanding the organization and regulation of eukaryotic spindle for accurate chromosome segregation in mitosis.

关键词： phase separation mitosis microtubule dynamics EB1 acetylation

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建构认同:新中国对外宣传六十年

山西大学学报(哲学社会科学版) 2011年第1期34卷 93-97页

作者：姚遥清华大学新闻与传播学院

过去一个甲子,国际局势风云变幻,为了谋求独立、生存与发展,借助大众传媒与人际交流,新中国与外部世界展开了持续不断却又千回百转的对外宣传与传播活动。通过回顾历史与论述现实,文章期待揭示:对外宣传不仅只是呈现于报纸、杂志或是广... 详细信息

过去一个甲子,国际局势风云变幻,为了谋求独立、生存与发展,借助大众传媒与人际交流,新中国与外部世界展开了持续不断却又千回百转的对外宣传与传播活动。通过回顾历史与论述现实,文章期待揭示:对外宣传不仅只是呈现于报纸、杂志或是广播、电视上的简单的媒介内容,实质上包含着一连串环环相扣、错综复杂的历史事件;对外宣传不仅只关乎新中国近60多年的时势变迁,还深植于中华民族5000多年的历史传统,更承载着近一个多世纪以来中国人追求现代化和重建国家认同的双重梦想;对外宣传不仅只与中国自身的发展休戚相关,更由于其沟通中外的独特作用,曾经深刻影响了国际格局的分合变幻,并不断改变着中华民族在世界舞台上的国际地位与身份认同。

关键词：新中国六十年对外宣传现代化国家认同

教育是一门科学吗?——美国教育学的早期探索

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山西大学学报(哲学社会科学版) 2023年第3期46卷 102-113页

作者：陈瑶云南师范大学教育学部

从19世纪后期美国教育学科初建之始，“教育是一门科学吗?”的追问就如影随形。19世纪后期到20世纪初期半个世纪的时段内，这样的争议经历了四个阶段。师范时代对“教育科学”的可能性饱含热望，但“热情多于理智”;初入大学，教育科学... 详细信息

从19世纪后期美国教育学科初建之始，“教育是一门科学吗?”的追问就如影随形。19世纪后期到20世纪初期半个世纪的时段内，这样的争议经历了四个阶段。师范时代对“教育科学”的可能性饱含热望，但“热情多于理智”;初入大学，教育科学在教育学教授努力论证的同时，遭遇着大学其他学术领域的普遍质疑，以及面临着来自观念、地域、性别和现实中的种种问题；在大学站稳脚跟后，争议的高潮来自教育研究者内部的争锋，历经多样化的“科学”探索和竞争；20年代后，随着美国大学中的教育专业学院的普遍建立，适应教育市场的实证量化研究成为教育科学的显性表征。这是美国的哲学传统、时代潮流、文化价值和知识政治综合作用的结果。然而，没有永远的“完胜者”,关于教育学科学性的质疑和争论在此后的一个多世纪中其实未见消停。这是教育研究及教育知识的特性使然，也是教育科学研究继续走向成熟的必然。

关键词：教育科学教育研究定量实证研究杜威桑代克

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新时期低碳经济的内涵与发展趋势分析

山西大学学报(哲学社会科学版) 2011年第4期34卷 140-144页

作者：姚逊山西大学科学技术哲学研究中心

化石燃料的过度消耗引发了全球性气温变暖,世界各国为解决气候问题广泛采用了低碳经济发展模式加以应对。文章对新时期低碳经济发展的内涵与趋势进行了详尽的分析,指出低碳经济既是一种关注温室气体排放的绿色经济论,同时也是一种全新... 详细信息

化石燃料的过度消耗引发了全球性气温变暖,世界各国为解决气候问题广泛采用了低碳经济发展模式加以应对。文章对新时期低碳经济发展的内涵与趋势进行了详尽的分析,指出低碳经济既是一种关注温室气体排放的绿色经济论,同时也是一种全新的经济形态,并分别对产业格局下与国际格局下的低碳经济的发展趋势进行了细致的解读。文章认为低碳经济发展能够带来产业结构、能源结构与消费结构的调整,继而引发经济发展方式的转型。

关键词：低碳经济低碳排放经济转型

客观性的悖论与话语权力的博弈——论现实主义无法回避的现实

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山西大学学报(哲学社会科学版) 2017年第2期40卷 34-42页

作者：路遥王耿太原师范学院文学院南京大学文学院

作为文艺理论的现实主义百年来在中国走过了坎坷的历程,曾经被奉为圭臬,如今却遭遇冷淡,何以至此?文章从现实主义文艺思潮和美学范型两个范畴,剖析其中复杂的内涵与外延。现实主义的美学范型其实是存在"悖论"的,它被裹挟进现实主义文艺... 详细信息

作为文艺理论的现实主义百年来在中国走过了坎坷的历程,曾经被奉为圭臬,如今却遭遇冷淡,何以至此?文章从现实主义文艺思潮和美学范型两个范畴,剖析其中复杂的内涵与外延。现实主义的美学范型其实是存在"悖论"的,它被裹挟进现实主义文艺思潮后,话语背后的权力博弈更徒增了现实主义的种种尴尬,确实是剪不断理还乱,让人厌倦。新时期以来又遭到了现代主义的挑战,表面上似乎该和现实主义的"话语纠缠"说再见了,然而受制于认识的进化,在相当长的历史时期内现实主义不可能也不会离开我们。

关键词：现实主义文艺思潮美学范型客观性话语权

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论沈从文性爱书写中的文化意蕴

山西大学学报(哲学社会科学版) 2005年第6期28卷 97-99页

作者：洪耀辉丽水广播电视大学浙江丽水323000

沈从文有相当部分的小说,是以人类两性之爱作为表现内容和审美对象。这些艺术文本中的性爱书写,通过对人类本质存在的两性之爱的潜质的挖掘,探究重建民族文化途径,揭示人类文明发展规律,呈示出丰厚的文化意蕴。

关键词：沈从文性爱书写:文化意蕴