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3D-printed porous functional composite scaffolds with polydopamine decoration for bone regeneration

Regenerative Biomaterials 2023年第1期10卷 1233-1247页

作者： Jinn Qi Yili Wang Liping Chen Linjie Chen Feng Wen Lijiang Huang pfukwa rueben Chunwu Zhang Huaqiong Li Department of Orthopaedics Joint Centre of Translational MedicineThe First Affiliated Hospital of Wenzhou Medical UniversityWenzhouZhejiang 325035P.R.China Joint Centre of Translational Medicine Zhejiang Engineering Research Center for Tissue Repair MaterialsWenzhou InstituteUniversity of Chinese Academy of SciencesWenzhouZhejiang 325011P.R.China University of Chinese Academy of Sciences Beijing 100049P.R.China The Second Affiliated Hospital of Wenzhou Medical University WenzhouZhejiang 325035P.R.China The Affiliated Xiangshan Hospital of Wenzhou Medical University NingboZhejiang 315700P.R.China Department of Chemistry and Polymer Science Stellenbosch UniversityMatielandStellenbosch 7602South Africa

Large size bone defects affect human health and remain a worldwide health problem that needs to be solved immediately.3D printing technology has attracted substantial attention for preparing penetrable multifunctional scaffolds to promote bone reconditioning and *** by the spongy structure of natural bone,novel porous degradable scaffolds have been printed using polymerization of lactide and caprolactone(PLCL)and bioactive glass 45S5(BG),and polydopamine(PDA)was used to decorate the PLCL/BG *** physicochemical properties of the PLCL/BG and PLCL/BG/PDA scaffolds were measured,and their osteogenic and angiogenic effects were characterized through a series of experiments both in vitro and in *** results show that the PLCL/BG2/PDA scaffold possessed a good compression modulus and brilliant *** proliferation,adhesion and osteogenesis of hBMSCs were improved in the PDA coating groups,which exhibited the best *** results of the SD rat cranium defect model indicate that PLCL/BG2/PDA obviously promoted osteointegration,which was further confirmed through immunohistochemical ***,PDA decoration and the sustained release of bioactive ions(Ca,Si,P)from BG in the 3D-printed PLCL/BG2/PDA scaffold could improve surface bioactivity and promote better osteogenesis and angiogenesis,which may provide a valuable basis for customized implants in extensive bone defect repair applications.

关键词： 3D printing bioactive glass composites polydopamine angiogenesis bone regeneration

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Cellular antioxidant and viability efficacy of Harpephyllum caffrum peel and Syzygium guineense seed extracts

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Food Bioscience 2022年第5期49卷 1042-1050页

作者： Trust M.pfukwa Wihan Pheiffer Olaniyi A.Fawole Marena Manley Cletos Mapiye Department of Food Science Faculty of AgriSciencesStellenbosch UniversityPrivate Bag X1MatielandStellenbosch7602South Africa DSI/NWU Preclinical Drug Development Platform Faculty of Health SciencesNorth-West UniversityPrivate Bag X6001Potchefstroom2520South Africa Postharvest Research Laboratory Department of Botany and Plant BiotechnologyFaculty of ScienceUniversity of JohannesburgPrivate Bag 524Auckland Park2006South Africa Department of Animal Sciences Faculty of AgriSciencesStellenbosch UniversityPrivate Bag X1MatielandStellenbosch7602South Africa

Crude phenolic extracts of indigenous sub-Saharan fruit byproducts have potent antioxidant *** of these extracts and evaluation of their antioxidant activity and viability using appropriate biological models,has,however,not yet been *** current study quantitatively evaluated the phenolic composition and in vitro antioxidant activity of crude and purified extracts(PE)of Harpephyllum caffrum peel(HCP)and Syzygium guineense seed(SGS),and cellular antioxidant and viability efficacy of the *** were extracted by 60%ethanol(v/v)solvent system and purified using XAD7HP macroporous adsorption *** UPLC/Q-TOF-MS was used for the characterization and identification of *** antioxidant activity(CAA)assay was applied to assess the antioxidant efficacy and cytotoxicity of the *** of variance of data was done using a generalized linear mixed model with fruit type,extract purity and their interaction as fixed *** increased(P≤0.05)HCP total phenolic concentration(TPC)by over 200%,while SGS TPC decreased(P≤0.05)by 20%.Regardless of fruit type,PE had higher(P≤0.05)quantities of proanthocyanidins and anthocyanins than crude *** HCP-PE had greater(P≤0.05)in vitro antioxidant activity than the other extracts(P≤0.05).Cellular antioxidant activity of HCP-PE was comparable to that of sodium metabisulphite(P>0.05).Also,HCP-PE had lower(P≤0.05)cytotoxic effects than SGS and sodium metabisulphite with increasing antioxidant *** was concluded that HCP-PE is a potential source of safe natural antioxidants for nutraceutical applications.

关键词： Harpephyllum caffrum Syzygium guineense Cellular antioxidant activity Fruit byproducts UPLC-MS Purification

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Oxidative preservative and sensory effects of Harpephyllum caffrum (wild plum) peel extracts in fresh ground beef patties

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FOOD BIOSCIENCE 2023年第3期53卷 1762-1771页

作者： pfukwa, Trust M. Chikwanha, Obert C. Marais, Jeannine Fawole, Olaniyi A. Manley, Marena Mapiye, Cletos Stellenbosch Univ Fac Agrisci Dept Food Sci Private Bag 11 ZA-7602 Stellenbosch South Africa Univ Johannesburg Fac Sci Dept Bot & Plant Biotechnol Private Bag 524Auckland Pk ZA-2006 Johannesburg South Africa Stellenbosch Univ Fac Agrisci Dept Anim Sci Private Bag 11 ZA-7602 Stellenbosch South Africa

Indigenous, underutilized fruits-byproducts are potential sources of novel antioxidants for the food industry. The current study evaluated the effects of different levels of purified ethanolic extracts of Harpephyllum caffrum Bernh peel (HCP, 0, 250, 450 and 650 ppm) on shelf life and sensory attributes of fresh ground beef patties stored at 4 degrees C during 9 days in comparison with sodium metabisulfite (SMB, 450 ppm). Antioxidant type x day in-teractions influenced (P <= 0.05) shelf life parameters, including loss of colour, myoglobin and protein oxidation. The HCP extracts improved colour and protein (i.e., reduced thiol loss) stability during storage compared to the control. The HCP treatments had lower (P <= 0.05) TBARS on day 9 compared to the control. Also, HCP reduced (P <= 0.05) tenderness and juiciness, and increased undesirable flavours in beef patties compared to control and SMB. The study indicates that HCP is a potential alternative to SMB in improving oxidative shelf life of the beef patties, with additional studies recommended to reduce adverse effects on sensory quality.

关键词： Harpephyllum caffrum Indigenous fruit extract Natural antioxidant Bitter flavour Descriptive analysis

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