Cellular antioxidant and viability efficacy of Harpephyllum caffrum peel and Syzygium guineense seed extracts

作     者：Trust M.Pfukwa Wihan Pheiffer Olaniyi A.Fawole Marena Manley Cletos Mapiye 

作者机构：Department of Food ScienceFaculty of AgriSciencesStellenbosch UniversityPrivate Bag X1MatielandStellenbosch7602South Africa DSI/NWU Preclinical Drug Development PlatformFaculty of Health SciencesNorth-West UniversityPrivate Bag X6001Potchefstroom2520South Africa Postharvest Research LaboratoryDepartment of Botany and Plant BiotechnologyFaculty of ScienceUniversity of JohannesburgPrivate Bag 524Auckland Park2006South Africa Department of Animal SciencesFaculty of AgriSciencesStellenbosch UniversityPrivate Bag X1MatielandStellenbosch7602South Africa 

出 版 物：《Food Bioscience》 (食品生物科学（英文）)

年 卷 期：2022年第49卷第5期

页      面：1042-1050页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Stellenbosch Gardens and Kirstenbosch Gardens for their support.Financial support for the research was provided by the South African Research Chairs Initiative(SARChI)in Meat Science:Genomics to Nutrinomics as well as the South African Department of Science and Technology(UID:84633) as directed by the NRF of South Africa.TMP would also like to thank SARChI for bursary support 

主　　题：Harpephyllum caffrum Syzygium guineense Cellular antioxidant activity Fruit byproducts UPLC-MS Purification 

摘      要：Crude phenolic extracts of indigenous sub-Saharan fruit byproducts have potent antioxidant *** of these extracts and evaluation of their antioxidant activity and viability using appropriate biological models,has,however,not yet been *** current study quantitatively evaluated the phenolic composition and in vitro antioxidant activity of crude and purified extracts(PE)of Harpephyllum caffrum peel(HCP)and Syzygium guineense seed(SGS),and cellular antioxidant and viability efficacy of the *** were extracted by 60%ethanol(v/v)solvent system and purified using XAD7HP macroporous adsorption *** UPLC/Q-TOF-MS was used for the characterization and identification of *** antioxidant activity(CAA)assay was applied to assess the antioxidant efficacy and cytotoxicity of the *** of variance of data was done using a generalized linear mixed model with fruit type,extract purity and their interaction as fixed *** increased(P≤0.05)HCP total phenolic concentration(TPC)by over 200%,while SGS TPC decreased(P≤0.05)by 20%.Regardless of fruit type,PE had higher(P≤0.05)quantities of proanthocyanidins and anthocyanins than crude *** HCP-PE had greater(P≤0.05)in vitro antioxidant activity than the other extracts(P≤0.05).Cellular antioxidant activity of HCP-PE was comparable to that of sodium metabisulphite(P0.05).Also,HCP-PE had lower(P≤0.05)cytotoxic effects than SGS and sodium metabisulphite with increasing antioxidant *** was concluded that HCP-PE is a potential source of safe natural antioxidants for nutraceutical applications.