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Participation of CD45,NKR-P1A and ANK61 antigen in rat hepatic NK cell(pit cell)-mediated target cell cytotoxicity

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World Journal of Gastroenterology 2000年第4期6卷 546-552页

作者： David Vermiilen bülent Ahishali Vasilis Triantis Karin Vanderkerken peter J. K.Kuppen Eddie Wisse Laboratory for Cell biology and Histology. Department of Hematology and immunology Free University of Brussels(VUB)Brussels-JetteBelgium K Vanderkerken is a postdoctoral researcher of the Fund for Scientific Research Flanders. Department of Surgery and Pathology Leiden University Medical CenterLeidenThe Netherlands

AIM Several triggering receptors have beendescribed to be involved in natural killer（NK）cell-mediated target *** these studies,NKcells derived from blood or spleen were *** are liver-specific NK cells that possess ahigher level of natural cytotoxicity and a differentmorphology when compared to blood NK *** aim of this study was to characterize the roleof the NK-triggering molecules NKR-P1A,ANK61antigen,and CD45 in pit cell-mediated killing oftarget *** 51Cr-release and DNA fragmentationwere used to quantify target cell lysis andapoptosis,*** Flow cytometric analysis showed thatpit cells expressed CD45,NKR-P1A,and *** of pit cells with monoclonalantibody（mAb）to CD45（ANK74）not onlyinhibited CC531s or YAC-1 target lysis but alsoapoptosis induced by pit *** mAbs to NKR-P1A（3.2.3）and ANK61 antigen（ANK61）had no effect on pit cell-mediated CC531s or YAC-1 targetcytolysis or apoptosis,while they did increase theFcγ receptor positive（FcγR+）P815 cytolysis *** enhanced cytotoxicity could beinhibited by 3,4-dichloroisocoumarin,an inhibitorof *** These results indicate that CD45participates in pit cell-mediated CC531s and YAC-1target cytolysis and ***-P1A andANK61 antigen on pit cells function as activationstructures against FcγR+ P815 cells,which wasmediated by the perforin/granzyme pathway.

关键词： hepatic NK cells pit cells cytolysis apoptosis perforin/granzyme pathway

Development of a sample preparation approach to measure the size of nanoparticle aggregates by electron microscopy

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Particuology 2019年第4期17卷 49-57页

作者： Agnieszka Dudkiewicz Angela Lehner Qasim Chaudhry Kristian Molhave Guenter Allmaier Karen Tiede Alistair b.A. boxall peter Hofmann John Lewis The Food and Environment Research Agency Sand Hutton York Y041 1LZ UK Department of Environment The University of York Heslington York YO10 5DD UK National Centre for Food Manufacturing The University of Lincoln Holbeach PE12 7AG UK Research Group bio- and Polymer Analysis Institute of Chemical Technologies and Analytics TU Wien (Vienna University of Technology) Getreidemarkt 9 A-1060 Vienna Austria University of Chester Parkgate Road Chester CH1 4BJ UK Department of Micro and Nanotechnology Technical University of Denmark DTU Bldg 345b Lyngby 2800 Denmark Research Centre for Global Food Security and Ecosystems University of Hohenheim Wollgrasweg 43 70155 Stuttgart Germany Department of Geoinformatics - Z.GIS University of Salzburg Schillerstr. 30 A-5020 Salzburg Austria

Electron microscopy (EM) is widely used for nanoparticle (NP) sizing. Following an initial assessment of two sample preparation protocols described in the current literature as"unperturbed", we found that neither could accurately measure the size of NPs featuring a broad size distribution, e.g., aggregates. because many real-world NP samples consist of aggregates, this finding was of considerable concern. The data showed that the protocols introduced errors into the measurement by either inducing agglomeration artefacts or providing a skewed size distribution towards small particles (skewing artefact). The focus of this work was to develop and apply a mathematical refinement to correct the skewing artefact. This refinement provided a much improved agreement between EM and a reference methodology, when applied to the measurement of synthetic amorphous silica NPs. Further investigation, highlighted the influence of NP chemistry on the refinement. This study emphasised the urgent need for greater and more detailed consideration regarding the sample preparation of NP aggregates to routinely achieve accurate measurements by EM. This study also provided a novel refinement solution applicable to the size characterisation of silica and c让rate-coated gold NPs featuring broad size distributions. With further research, this approach could be extended to other NP types.

关键词： Nanoparticles Aggregates Measurement Electron microscopy Sample preparation Artefacts

Canopy gap size influences niche partitioning of the ground-layer plant community in a northern temperate forest

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Journal of Plant Ecology 2013年第1期6卷 92-100页

作者： Christel C.Kern Rebecca A.Montgomery peter b.Reich Terry F.Strong USDA Forest Service Northern Research Station1831 Hwy 169 EastGrand RapidsMN 55744USA Department of Forest Resources University of Minnesota-Twin Cities1530 North Cleveland AvenueSaint PaulMN 55108USA Hawkesbury Institute for the Environment University of Western SydneyLocked Bag 1797Penrith NSW 2751Australia USDA Forest Service USARetired

Aims The Gap Partitioning Hypothesis(GPH)posits that gaps create heterogeneity in resources crucial for tree regeneration in closed-canopy forests,allowing trees with contrasting strategies to coexist along resource *** studies have examined gap partitioning of temperate,ground-layer vascular *** used a ground-layer plant community of a temperate deciduous forest in northern Wisconsin,USA,as a model system to test whether the GPH extends to the relatively species-rich ground *** We used a well-replicated experimental approach that included a gap opening gradient(five gap sizes,6,10,20,30 and 46 m diameter,and undisturbed reference areas),a within-gap location gradient(gap edge to center),and a temporal gradient(0,2,6 and 13 years after gap creation).The data were observations of ground-layer plant abundance,published plant traits,and a modeled index of understory light *** ordinated the plant abundance data and evaluated the relationships of composition,traits and light environment by gap size,location along the forest-gap transect and time,with several approaches such as correlations,descriptive statistics,non-parametric tests of group differences and indicator species importance *** Findings Ground-layer plant composition and traits differed across gap sizes,within-gap locations and over *** of all sizes differed in composition from undisturbed areas,and all pair-wise combinations of gap size also differed in composition,except the 6 m from the 10-m *** gaps(46 m)also displayed within-gap compositional gradients from gap edge to center *** differences in gap size were evident 2 years after gap creation and,contrary to our hypotheses,remained different over the 13-year period,even in gaps with crown *** contrast to the neutral theory,species functional traits and microenvironmental conditions were related to variation in ground-layer *** with smaller seeds,low

关键词： herbaceous layer experimental gaps proximity to edge functional diversity plant traits

Grid Integration of Wind Generation Considering Remote Wind Farms:Hybrid Markovian and Interval Unit Commitment

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IEEE/CAA Journal of Automatica Sinica 2017年第2期4卷 205-215页

作者： bing Yan Haipei Fan peter b.Luh Khosrow Moslehi Xiaoming Feng Chien Ning Yu Mikhail A.bragin Yaowen Yu IEEE Department of Electrical & Computer Engineering University of Connecticut University of Connecticut Abb Inc. Abb Enterprise Software

Grid integration of wind power is essential to reduce fossil fuel usage but challenging in view of the intermittent nature of ***,we developed a hybrid Markovian and interval approach for the unit commitment and economic dispatch problem where power generation of conventional units is linked to local wind states to dampen the effects of wind ***,to reduce complexity,extreme and expected states are considered as interval *** this approach is effective,the fact that major wind farms are often located in remote locations and not accompanied by conventional units leads to conservative ***,weights of extreme and expected states in the objective function are difficult to tune,resulting in significant differences between optimization and simulation *** this paper,each remote wind farm is paired with a conventional unit to dampen the effects of wind uncertainties without using expensive utility-scaled battery storage,and extra constraints are innovatively established to model ***,proper weights are derived through a novel quadratic fit of cost *** problem is solved by using a creative integration of our recent surrogate Lagrangian relaxation and *** demonstrate modeling accuracy,computational efficiency,and significant reduction of conservativeness of the previous approach.

关键词： branch-and-cut interval optimization Markov decision process remote wind farms surrogate Lagrangian relaxation(SLR) unit commitment

Synergistic effects of interferon-alpha in combination with chemoradiation on human pancreatic adenocarcinoma

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World Journal of Gastroenterology 2005年第10期11卷 1521-1528页

作者： Jian-Hua Ma Emilia Patrut Jan Schmidt Hanns-peter Knaebel Markus W. büchler Angela M(a|¨)rten Department of Surgery

AIM: To determine whether IFN-α is the agent that turns a slightly effective treatment (radiochemotherapy) into a potent therapy, we tested IFN-α for its synergistic ***: Eight pancreatic carcinoma cell lines were treated with the single agents and combinations of *** role of IFN-α regarding a) direct inhibitory effects; b)radio and chemosensitizing effects; c) anti-angiogenic properties and d) enhancement of immunogenicity was ***: Our results show that IFN-α has direct inhibitory properties and some synergistic influence as determined by AnnexinV/PI stain and cell count. IFN-α is also able to prevent the increase in proliferation rate and VEGF secretion of CDDP resistant cells. Having taken the results from immunogenicity experiments together, we found cells that can be influenced by IFN-α but were less susceptible against T cells. Furthermore, high expression of MHC molecules, CD118, EGF-R and Fas was predictive for a good ***: In conclusion, IFN-α has direct cytotoxic effects, acts as a radiosensitizer and circumvents tumor cell-regrowth after CDDP treatment. These mechanisms may be responsible for the good clinical outcome of CapRI.

关键词： Interferon-alpha Pancreatic adenocarcinoma Synergistic effects Combination therapy

Chlorophyll, anthocyanin, and gas exchange changes assessed by spectroradiometry in Fragaria chiloensis under salt stress

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Journal of Integrative Plant biology 2014年第5期56卷 505-515页

作者： Miguel Garriga Jorge b. Retamales Sebastián Romero-bravo peter D.S. Caligari Gustavo A. Lobos Faculty of Agricultural Sciences Plant Breeding and Phenomic Center Universidad de Talca Instituto de biología Vegetaly biotecnología Universidad de Talca

Chlorophyll and anthocyanin contents provide a valuable indicator of the status of a plant’s physiology, but to be more widely utilized it needs to be assessed easily and non‐destructively. This is particularly evident in terms of assessing and exploiting germplasm for plant‐breeding programs. We report, for the first time, experiments with Fragaria chiloensis（L.）Duch. and the estimation of the effects of response to salinity stress（0, 30, and 60 mmol NaCl/L） in terms of these pigments content and gas exchange. It is shown that both pigments（which interestingly, themselves show a high correlation） give a good indication of stress response. both pigments can be accurately predicted using spectral reflectance indices（SRI）;however, the accuracy of the predictions was slightly improved using multilinear regression analysis models and genetic algorithm analysis. Specifically for chlorophyll content, unlike other species, the use of published SRI gave better indications ofstress response than Normalized Difference Vegetation *** effect of salt on gas exchange is only evident at the highest concentration and some SRI gave better prediction performance than the known Photochemical Reflectance Index. This information will therefore be useful for identifying tolerant genotypes to salt stress for incorporation in breeding programs.

关键词： Gas exchange high‐throughput phenotyping pigment phenomic photosynthesis reflectance spectral reflectance indices

围术期神经肌肉阻滞的管理:欧洲麻醉和重症监护学会临床指南

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中华麻醉学杂志 2024年第6期44卷 641-656页

作者： Thomas Fuchs-buder Carolina S.Romero Heidrun Lewald Massimo Lamperti Arash Afshari Ana-Marjia Hristovska Denis Schmartz Jochen Hinkelbein Dan Longrois Maria Popp Hans Dde boer Massimiliano Sorbello Radmilo Jankovic peter Kranke 法国南锡大学医学院附属医院麻醉学、重症监护与围术期医学科法国南锡54000 巴伦西亚大学综合医院麻醉与重症监护科西班牙巴伦西亚46014 慕尼黑工业大学麻醉学与重症监护科德国慕尼黑80333 克利夫兰阿布扎比诊所麻醉学研究所阿联酋阿布扎比31250 哥本哈根大学医院小儿和产科麻醉科丹麦哥本哈根2100 哥本哈根大学医院麻醉学与重症监护科丹麦赫维德2650 布鲁塞尔自由大学Erasme医院麻醉科比利时布鲁塞尔1070 科隆大学医院麻醉学与重症监护科德国科隆50937 巴黎大学附属比查特克劳德伯纳德医院麻醉学与重症监护医学科法国巴黎75018 维尔茨堡大学医院麻醉学、危重病医学、急诊医学和疼痛医学科德国维尔茨堡97080 格罗宁根大学医院麻醉学、疼痛医学与程序性镇静与镇痛科荷兰格罗宁根9713 GZ 卡坦尼亚大学医院麻醉与重症监护科意大利卡坦尼亚95123 尼什大学医学院尼什临床中心麻醉学与重症治疗科塞尔维亚尼什18000

近来的研究表明,由于围术期神经肌肉阻滞管理不当,肌肉松弛药(肌松药)残留阻滞及其术后并发症的发生率居高不下。需要重视神经肌肉功能监测仪、肌松药及其拮抗剂的合理应用,以利于改善神经肌肉阻滞管理。欧洲麻醉和重症监护学会(Europea... 详细信息

近来的研究表明,由于围术期神经肌肉阻滞管理不当,肌肉松弛药(肌松药)残留阻滞及其术后并发症的发生率居高不下。需要重视神经肌肉功能监测仪、肌松药及其拮抗剂的合理应用,以利于改善神经肌肉阻滞管理。欧洲麻醉和重症监护学会(European Society of Anaesthesiology and Intensive Care,ESAIC)首次发布围术期神经肌肉阻滞管理临床指南,旨在为临床医生提供最佳医疗管理的循证建议,以提高围术期患者安全。本指南主要聚焦在3个临床问题:肌松药是否有助于成人气管插管?神经肌肉阻滞深度是否影响腹部手术患者的预后?肌松药残留阻滞的诊断和治疗策略有哪些?根据PICO原则(患者、干预、对照、结局;patient,intervention,comparator,outcome;PICO)构建临床问题并进行文献检索。初始纳入的临床研究为24 000篇,采用逐步筛选策略,最终临床研究88篇纳入指南分析。应用GRADE方法学(Grading of Recommendations,Assessment,Development and Evaluation,GRADE)对结局指标证据、质量等级进行评价。运用两轮德尔菲法收集专家组建议并形成统一推荐:R1推荐应用肌松药辅助气管插管(1A)。R2推荐应用肌松药以减少气管插管导致的咽部和(或)喉部损伤(1C)。R3推荐在快速顺序诱导插管(rapid sequence induction intubation,RSII)时使用短效肌松药,例如琥珀胆碱1 mg/kg或罗库溴铵0.9~1.2 mg/kg(1b)。R4推荐在需要改善外科手术条件时,加深神经肌肉阻滞深度(1b)。R5尚无足够的证据推荐深度神经肌肉阻滞可减轻术后疼痛或降低围术期并发症的发生率(2C)。R6推荐在拇内收肌处刺激尺神经,定量监测神经肌肉阻滞深度以排除肌松药残留阻滞(1b)。R7推荐应用舒更葡糖钠逆转甾体类肌松药(罗库溴铵、维库溴铵)诱导的深、中及浅度神经肌肉阻滞(1A)。R8推荐在使用新斯的明前,TOFr自行恢复至>0.2,并继续定量监测神经肌肉功能,直至TOFr超过0.9(1C)。

关键词：神经肌肉阻滞神经肌肉功能肌松药临床指南罗库溴铵琥珀胆碱外科手术定量监测

Association between calcium sensing receptor gene polymorphisms and chronic pancreatitis in a US population:Role of serine protease inhibitor Kazal 1type and alcohol

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World Journal of Gastroenterology 2008年第28期14卷 4486-4491页

作者： Venkata Muddana Janette Lamb Julia b Greer beth Elinoff Robert H Hawes peter b Cotton Michelle A Anderson Randall E brand Adam Slivka David C Whitcomb Division of Gastroenterology Hepatology and NutritionDepartment of MedicineUniversity of Pittsburgh Medical CenterPittsburgh PA 15213United States Digestive Disorder Center Medical University of South CarolinaCharleston SC 29403United States Department of Medicine University of Michigan Medical CenterAnn Arbor MI 48109United States Department of medicine Northwestern Universityand Currently at University of Pittsburgh Medical CenterPittsburgh PA 15213United States Division of Gastroenterology Hepatology and NutritionDepartment of MedicineUniversity of Pittsburgh Medical CenterPittsburgh PA 15213United States Department of Human GeneticsUniversity of PittsburghPittsburgh PA 15213United States

AIM： To test the hypothesis that calcium sensing receptor （CASR） polymorphisms are associated with chronic pancreatitis （CP）, and to determine whether serine protease inhibitor Kazal 1type （SPINK1） N34S or alcohol are necessary co-factors in its etiology. METHODS： Initially, 115 subjects with pancreatitis and 66 controls were evaluated, of whom 57 patients and 21 controls were predetermined to carry the high-risk SPINK1 N34S polymorphism. We sequenced CASR gene exons 2, 3, 4, 5 and 7, areas containing the majority of reported polymorphisms and novel mutations. based on the initial results, we added 223 patients and 239 controls to analyze three common nonsynonymous single nucleotide polymorphisms （SNPs） in exon 7 （A986S, R990G, and Q1011E）. RESULTS： The CASR exon 7 R990G polyrnorphism was significantly associated with CP （OR, 2.01; 95% CI, 1.12-3.59; P = 0.015）. The association between CASR R990G and CP was stronger in subjects who reported moderate or heavy alcohol consumption （OR, 3.12; 95% CI, 1.14-9.13; P = 0.018）. There was no association between the various CASR genotypes and SPINK1 N34S in pancreatitis. None of the novel CASR polymorphisms reported from Germany and India was detected. CONCLUSION： Our United States-based study confirmed an association of CASR and CP and for the first time demonstrated that CASR R990G is a significant risk factor for CP. We also conclude that the risk of CP with CASR R990G is increased in subjects with moderate to heavy alcohol consumption.

关键词： Calcium sensing receptor Serine protease inhibitor Kazal llype Chronic pancreatitis Alcohol

Multi-omic Analyses Shed Light on The Genetic Control of High-altitude Adaptation in Sheep

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Genomics, Proteomics & bioinformatics 2024年第2期22卷 43-56页

作者： Chao Li bingchun Chen Suo Langda Peng Pu Xiaojia Zhu Shiwei Zhou peter Kalds Ke Zhang Meenu bhati Alexander Leonard Shuhong Huang Ran Li Awang Cuoji Xiran Wang Haolin Zhu Yujiang Wu Renqin Cuomu ba Gui Ming Li Yutao Wang Yan Li Wenwen Fang Ting Jia Tianchun Pu Xiangyu Pan Yudong Cai Chong He Liming Wang Yu Jiang Jian-Lin Han Yulin Chen Ping Zhou Hubert Pausch Xiaolong Wang International Joint Agriculture Research Center for Animal bio-breeding Ministry of Agriculture and Rural Affairs/Key Laboratory of Animal GeneticsBreeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100China Animal Genomics ETH ZürichZürich 8092Switzerland Institute of Animal Science Tibet Academy of Agricultural and Animal Husbandry SciencesLhasa 850009China School of biological and Pharmaceutical Engineering Lanzhou Jiaotong UniversityLanzhou 730070China College of Life Sciences Shaanxi Normal UniversityXi’an 710119China Zoology and Evolutionary biology Department of BiologyUniversity of Konstanz78457 KonstanzGermany College of Life and Geographic Sciences Kashi UniversityKashi 844000China beijing Key Laboratory of Captive Wildlife Technologies Beijing ZooBeijing 100044China Department of Medical Research Guangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhou 510080China Key Laboratory of Agricultural Internet of Things Ministry of Agriculture and Rural Affairs/Shaanxi Key Laboratory of Agricultural Information Perception and Intelligent ServiceCollege of Information EngineeringNorthwest A&F UniversityYangling 712100China Institute of Animal Husbandry and Veterinary Medicine Xinjiang Academy of Agricultural and Reclamation SciencesShihezi 832000China State Key Laboratory of Sheep Genetic Improvement and Healthy Production Xinjiang Academy of Agricultural and Reclamation SciencesShihezi 832000China CAAS-ILRI Joint Laboratory on Livestock and Forage Genetic Resources Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijing 100193China Livestock Genetics Program International Livestock Research InstituteNairobi 00100Kenya

Sheep were domesticated in the Fertile Crescent and then spread globally,where they have been encountering various environmental *** Tibetan sheep has adapted to high altitudes on the Qinghai-Tibet Plateau over the past 3000 *** explore genomic variants associated with high-altitude adaptation in Tibetan sheep,we analyzed Illumina short-reads of 994 whole genomes representing∼60 sheep breeds/populations at varied altitudes,Pacbio High fidelity(HiFi)reads of 13 breeds,and 96 transcriptomes from 12 sheep *** testing between the inhabited altitudes and 34,298,967 variants was conducted to investigate the genetic architecture of altitude *** accurate HiFi reads were used to complement the current ovine reference assembly at the most significantly associatedβ-globin locus and to validate the presence of two haplotypes A and b among 13 sheep *** haplotype A carried two homologous gene clusters:(1)HbE1,HbE2,Hbb-like,and HbbC,and(2)HbE1-like,HbE2-like,Hbb-like,and Hbb;while the haplotype b lacked the first *** high-altitude sheep showed highly frequent or nearly fixed haplotype A,while the low-altitude sheep dominated by haplotype *** further demonstrated that sheep with haplotype A had an increased hemoglobin–O2 affinity compared with those carrying haplotype *** highly associated genomic region contained the EGLN1 gene which showed varied expression between high-altitude and low-altitude *** results provide evidence that the rapid adaptive evolution of advantageous alleles play an important role in facilitating the environmental adaptation of Tibetan sheep.

关键词： Environmental adaptation High altitude Hypoxia Selection signature Sheep