Participation of CD45,NKR-P1A and ANK61 antigen in rat hepatic NK cell(pit cell)-mediated target cell cytotoxicity
Participation of CD45,NKR-P1A and ANK61 antigen in rat hepatic NK cell(pit cell)-mediated target cell cytotoxicity作者机构:Laboratory for Cell Biology and Histology. Department of Hematology and immunologyFree University of Brussels(VUB)Brussels-JetteBelgium K Vanderkerken is a postdoctoral researcher of the Fund for Scientific Research Flanders. Department of Surgery and PathologyLeiden University Medical CenterLeidenThe Netherlands
出 版 物:《World Journal of Gastroenterology》 (世界胃肠病学杂志(英文版))
年 卷 期:2000年第6卷第4期
页 面:546-552页
核心收录:
学科分类:1002[医学-临床医学] 100201[医学-内科学(含:心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
基 金:the grants 3.0053.92,3.0050.95,9.0038.96,1.5.411.98 from the National Foundation for Scientific Research(FWO) the grants 194.322.1740,195.332.1310,196.322.0140,and OZR.230 from the Research Council of the Free University of Brussels
主 题:hepatic NK cells pit cells cytolysis apoptosis perforin/granzyme pathway
摘 要:AIM Several triggering receptors have beendescribed to be involved in natural killer(NK)cell-mediated target *** these studies,NKcells derived from blood or spleen were *** are liver-specific NK cells that possess ahigher level of natural cytotoxicity and a differentmorphology when compared to blood NK *** aim of this study was to characterize the roleof the NK-triggering molecules NKR-P1A,ANK61antigen,and CD45 in pit cell-mediated killing oftarget *** 51Cr-release and DNA fragmentationwere used to quantify target cell lysis andapoptosis,*** Flow cytometric analysis showed thatpit cells expressed CD45,NKR-P1A,and *** of pit cells with monoclonalantibody(mAb)to CD45(ANK74)not onlyinhibited CC531s or YAC-1 target lysis but alsoapoptosis induced by pit *** mAbs to NKR-P1A(3.2.3)and ANK61 antigen(ANK61)had no effect on pit cell-mediated CC531s or YAC-1 targetcytolysis or apoptosis,while they did increase theFcγ receptor positive(FcγR+)P815 cytolysis *** enhanced cytotoxicity could beinhibited by 3,4-dichloroisocoumarin,an inhibitorof *** These results indicate that CD45participates in pit cell-mediated CC531s and YAC-1target cytolysis and ***-P1A andANK61 antigen on pit cells function as activationstructures against FcγR+ P815 cells,which wasmediated by the perforin/granzyme pathway.