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C–H Arylation using acyl thiourea ligands: Applications in the synthesis of 3,6-diaryl-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazoles

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Chinese Chemical Letters 2016年第1期27卷 37-40页

作者： Aamer Saeed pervaiz ali channar Qasir Iqbal Jamaluddin Mahar Department of Chemistry Quaid-i-Azam University

Synthesis of a series of new 3,6-diaryl-[1,2,4]triazolo[3,4-b] [1,3,4]thiadiazoles（5a-o） was achieved by phophine free, C-H arylative cross-coupling of 6-aryl-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazoles（4a-o）with suitably substituted iodoanilines using 1-（2-naphthoyl）-3-（4-bromophenyl）thiourea as a *** requisite triazolothiadiazoles（4a-o） were obtained by the condensation of 4-amino-1,2,4-triazole-3-thiol（3） with suitably substituted aromatic acids in the presence of phosphoryl chloride.

关键词： Triazolo[3,4-b]thiadiazoles Acyl thiourea C-H arylative coupling N,N-Dimethylacetamide

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Assessing the response of indigenous loquat cultivar Mardan to phytohormones for in vitro shoot proliferation and rooting

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第9期14卷 774-784页

作者： Nadeem Akhtar ABBASI Tariq pervaiz Ishfaq Ahmed HAFIZ Mehwish YASEEN Azhar HUSSAIN Plant Tissue Culture Laboratory Department of HorticulturePir Mehr Ali Shah-Arid Agriculture University

In vitro cultures of loquat cultivar Mardan were established using shoot apices after treating with NaOCl (5%,7%,10%,12%,14% (v/v)) for 12 min and HgCl2 (0.01%,0.05%,0.10%,0.20%,0.25% (w/v)) for 2 min.A maximum survival rate of 70% was recorded after surface sterilization with 10% *** response was assessed on Murashige and Skoog (MS) medium fortified with assorted combinations of the cytokinins,benzylaminopurine (BAP),kinetin,and N6-(2-isopentyl)adenine (2iP).Treatment of BAP 1.5 mg/L combined with 2iP 9.0 mg/L and kinetin 1.5 mg/L was found to be optimum for shoot morphogenesis in terms of the number and subsequent growth of shoots,while the highest shoot length was yielded by the combination of BAP 0.5 mg/L,kinetin 0.5 mg/L,and 2iP 3 mg/*** levels of cytokinins induced callogenesis,vitrification and stunted growth to some *** rhizogenesis,uniform sized micro-shoots were excised and transferred to half-strength MS medium containing *** best rooting expression was observed with naphthaleneacetic acid (NAA) 1 mg/L combined with indole-3-butyric acid (IBA) 2 mg/L and paclobutrazol (PBZ) 1 mg/L.

关键词： Eriobotryajaponica Micropropagation Sterilization of Ioquat Plant growth regulator Shoot proliferation Rhizogenesis

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Nutrients Extracted from Chicken Manure Accelerate Growth of Microalga <i>Scenedesmus obliquus</i>HTB1

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Green and Sustainable Chemistry 2017年第2期7卷 101-113页

作者： Xiaotian Han Nicole Rusconi pervaiz ali Kevin Pagkatipunan Feng Chen Institute of Marine and Environmental Technology University of Maryland Center for Environmental Science Baltimore MD USA CAS Key Laboratory of Marine Ecology and Environmental Science Institute of Oceanography Chinese Academy of Sciences Qingdao China College of Marine Life Sciences Ocean University of China Qingdao China

Sustainable development using wastes as resources is a new paradigm. Chicken manure contains rich amounts of nitrogen and phosphorus and has been used as crop fertilizer. However, little is known about whether nutrients of chicken manure are suitable and efficient to support the rapid growth of microalgae. In this study, we explore the possibility of using nutrient extracted from chicken manure to grow microalgae. We used an algal strain Scenedesmus sp. HTB1, which is an oleaginous species with high CO2 tolerance capability. The growth performance of HTB1 on various media amended with nutrient extracted from three different chicken manure sources was monitored and compared to the growth rate of HTB1 grown in the standard medium BG11. Meanwhile, the changes of total nitrogen (N) and phosphorus (P), both organic and inorganic, were measured during the growth period. Culture media enriched with the nutrient extracted from two chicken manure sources outperformed the standard culture medium BG11 in terms of algal biomass production. When cultivated with manure nutrient, HTB1 utilized inorganic N efficiently, but consumed very little organic N during the experimental growth period. However, HTB1 was able to utilize both organic and inorganic phosphorus. We demonstrate that nutrient extracted from chicken manure support rapid growth and high biomass yield in microalgae?Scenedesmus obliquus?HTB1. Therefore chicken manure holds great promise to be used as a cost-effective and efficient fertilizer for large-scale production of microalgae.

关键词： Microalgae Scenedesmus Chicken Manure Nutrient Recycle

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Insight into the phytochemical, biological, and in silico studies of Erythrina suberosa roxb.: A source of novel therapeutic bioactive products from a medicinal plant

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Food Bioscience 2023年第2期52卷 1421-1432页

作者： Abdulhakeem S.Alamri Hammad Saleem Irfan pervaiz Umair Khurshid Juwairiya Butt Tahir ali Chohan Majid Alhomrani Marcello Locatelli Syafiq Asnawi Zainal Abidin Yasir Nasser H Alqahtani Kannan RRRengasamy Mohamad Fawzi Mahomoodally Department of Clinical Laboratory Sciences The Faculty of Applied Medical SciencesTaif UniversityTaifSaudi Arabia Centre of Biomedical Sciences Research(CBSR) Deanship of Scientific ResearchTaif UniversitySaudi Arabia Institute of Pharmaceutical Sciences(IPS) University of Veterinary&Animal Sciences(UVAS)LahorePakistan KITAAS Khaldunia College of PharmacyLahorePakistan Department of Pharmaceutical Chemistry Faculty of PharmacyThe Islamia University of Bahawalpur63100Pakistan School of Life Sciences University of Westminster115 New Cavendish StreetLondonW1W 6UWUK Department of Pharmacy University‘G.d’Annunzio of Chieti-Pescara66100ChietiItaly Jeffrey Cheah School of Medicine and Health Sciences Monash University MalaysiaJalan Lagoon Selatan47500Bandar SunwaySelangor Darul EhsanMalaysia MOI Clinics Security Forces HospitalRiyadhSaudi Arabia Laboratory of Natural Products and Medicinal Chemistry(LNPMC) Department of PharmacologySaveetha Dental CollegeSaveetha Institute of Medical and Technical Sciences(SIMATS)Chennai600077India Department of Health Sciences Faculty of Medicine and Health SciencesUniversity of MauritiusR´eduit80837Mauritius Centre of Excellence for Pharmaceutical Sciences North-West UniversityPrivate Bag X6001Potchefstroom2520South Africa

Background:Erythrina suberosa(***)*** known as corky coral tree is used as a folklore medicine to treat ulcers and *** study explored the phytochemical profiles of methanol and dichloromethane(DCM)extracts from the aerial,root,and flower parts of ***:Total bioactive contents,high-performance liquid chromatography array(HPLC-PDA)polyphenolic quantification,and ultra-high performance liquid chromatography-mass spectrometry(UHPLC-MS)analysis were used to evaluate the phytochemical *** potential was evaluated by determining their antioxidant(DPPH,ABTS,FRAP,CUPRAC,Phosphomolybdenum,and metal chelating)and enzyme inhibitory(cholinesterase,amylase,glucosidase,and tyrosinase)***:The phenolic(46.26 mg GAE/g extract)and flavonoid(32.46 mg QE/g extract)contents were found to be highest in the methanolic extract of roots(EsR-M).HPLC-PDA analysis determined the presence of numerous bioactive compounds,including catechin,syringic acid,p-hydroxy benzoic acid,gallic acid,vanillic acid,epicatechin,rutin,chlorogenic acid,carvacrol,sinapinic acid,and naringenin in considerable amounts.A total of 143 phytochemicals,belonging to phenolic,flavonoid,coumarin,terpenoid,chalcone,saponin,and glycoside classes,were tentatively identified by UHPLC-MS *** methanolic extract(EsA-M)exhibited highest radical scavenging(DPPH 86.81 mgTE/g extract,ABTS 245.17 mgTE/g extract)as well as reducing potential(FRAP 177.12 mgTE/g extract,CUPRAC 177.12 mgTE/g extract).Likewise,it was also noted to be most active for BChE(5.70 mg GALAE/g extract)and tyrosinase inhibition(148.35 mg KAE/g extract).While,roots-DCM extract(EsR-D)presented considerable inhibition for AChE(4.69 mg GALAE/g extract)and amylase(0.81 mmol ACAE/g extract)***,davallioside A,and isovitexin were docked to tyrosinase to depict possible ***:This study has established baseline data on the biological and phytochemical profile of ***

关键词： Erythrina suberosa HPLC-PDA UHPLC-MS Antioxidant Enzyme inhibition Natural products

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Outcome ofJudet＇s quadricepsplasty for knee contractures and the effect of local infiltration of epinephrine on reducing blood loss

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Chinese Journal of Traumatology 2017年第3期20卷 147-150页

作者： Akbar Jaleel Zubairi Haroon Rashid Rizwan Haroon Rashid Moiz ali pervaiz Mehmood Hashmi Section of Orthopedics Department of Surgery Ago Khan University Hospital Karachi 74800 Pakistan Aga Khan University Medical College Karachi 74800 Pakistan

Objective： To evaluate the effectiveness ofJudet＇s quadricepsplasty for treatment of knee contractures and to identify the effect of local infiltration of epinephrine on blood loss associated with this procedure. Methods： A retrospective cohort study was conducted in which all cases of knee contractures managed with Judet＇s quadricepsplasty from 1st January 2009 to 31st December 2013 were included and were divided into two groups. The epinephrine group included patients who were infiltrated with diluted epinephrine （1：400,000） along with xylocaine, around the operative field 15 min prior to the incision time, while the control group did not receive any infiltration. Judet＇s outcome, blood loss, drop in he- moglobin and required blood transfusion were noted for all patients and compared between both groups. Results： Most common preceding pathology identified for the development of knee contractures was periarticular fracture while ilizarov application was the most common etiology. Both groups were found similar in all preoperative characteristics except preoperative flexion contracture （p = 0.02）. All func- tional outcome measures including Judet＇s outcome were similar in both groups. In contrast, duration of surgery （p = 0.01）, blood loss （p = 0.02）, drop in hemoglobin （p = 0.01） and number of transfusions （p = 0.03） were significantly reduced in epinephrine group. Conclusion： Judet＇s quadricepsplasty is a useful procedure to increase the range of motion of rigid knees and local infiltration of epinephrine is effective in decreasing the amount of subsequent blood loss and transfusion requirements.

关键词： Knee contracture Blood transfusion Epinephrine Knee range of motion Operative time

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