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Reorganization of 3D genome architecture across wild boar and Bama pig adipose tissues

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Journal of Animal Science and Biotechnology 2022年第4期13卷 1011-1027页

作者： Jiaman Zhang pengliang liu Mengnan He Yujie Wang Hua Kui Long Jin Diyan Li Mingzhou Li Institute of Animal Genetics and Breeding College of Animal Science and TechnologySichuan Agricultural UniversityChengdu 611130China

Background:A growing body of evidence has revealed that the mammalian genome is organized into hierarchical layers that are closely correlated with and may even be causally linked with variations in gene *** studies have characterized chromatin organization in various porcine tissues and cell types and compared them among species and during the early development of ***,how chromatin organization differs among pig breeds is poorly ***:In this study,we investigated the 3D genome organization and performed transcriptome characterization of two adipose depots(upper layer of backfat[ULB]and greater omentum[GOM])in wild boars and Bama pigs;the latter is a typical indigenous pig in *** found that over 95%of the A/B compartments and topologically associating domains(TADs)are stable between wild boars and Bama *** contrast,more than 70%of promoterenhancer interactions(PEIs)are dynamic and widespread,involving over a thousand *** in chromatin structure are associated with changes in the expression of genes that are involved in widespread biological functions such as basic cellular functions,endocrine function,energy metabolism and the immune *** 95%and 97%of the genes associated with reorganized A/B compartments and PEIs in the two pig breeds differed between GOM and ULB,***:We reported 3D genome organization in adipose depots from different pig *** a comparison of Bama pigs and wild boar,large-scale compartments and TADs were mostly conserved,while fine-scale PEIs were extensively *** chromatin architecture in these two pig breeds was reorganized in an adipose depotspecific *** results contribute to determining the regulatory mechanism of phenotypic differences between Bama pigs and wild boar.

关键词： 3D genome organization A/B compartments Adipose tissues PEI Pig breeds TAD

Azobenzene-based ultrathin peptoid nanoribbons for the potential on highly efficient artificial light-harvesting

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Science China Chemistry 2024年第5期67卷 1680-1686页

作者： Zichao Sun Lingrong Zhu Jingze liu Fan liu Pengchao Wu pengliang Sui Haibao Jin Shaoliang Lin Shanghai Key Laboratory of Advance Polymeric Materials Frontiers Science Center for Materiobiology and Dynamic ChemistrySchool of Materials Science and EngineeringEast China University of Science and TechnologyShanghai 200237China

The development of artificial light-harvesting systems based on long-range ordered ultrathin organic nanomaterials(i.e., below3 nm), which were assembled from stimuli-responsive sequence-controlled biomimetic polymers, remains challenging. Herein,we report the self-assembly of azobenzene-containing amphiphilic ternary alternating peptoids to construct photo-responsive ultrathin peptoids nanoribbons(UTPNRs) with a thickness of ~2.3 nm and the length in several micrometers. The pendants hydrophobic conjugate stacking mechanism explained the formation of one-dimensional ultrathin nanostructures, whose thickness was highly dependent on the length of side groups. The photo-isomerization of azobenzene moiety endowed the aggregates with a reversible morphology transformation from UTPNRs to spherical micelles(46.5 nm), upon the alternative irradiation with ultraviolet and visible light. Donor of 4-(2-hydroxyethylamino)-7-nitro-2,1,3-benzoxadiazole(NBD) and acceptor of rhodamine B(RB) were introduced onto the hydrophobic and hydrophilic regions, respectively, to generate photocontrollable artificial light-harvesting systems. Compared with the spheres-based systems, the obtained NBD-UTPNRs@RB composite proved a higher energy transfer efficiency(90.6%) and a lower requirement of RB acceptors in water. A proof-ofconcept use as fluorescent writable ink demonstrated the potential of UTPNRs on information encryption.

关键词： azobenzene-based peptoid ultrathin nanoribbon photo-responsive light-harvesting information encryption

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Dynamics and functionalities of bacterial community during foxtail millet dough fermentation by metagenomic analysis

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Journal of Future Foods 2024年第4期4卷 343-352页

作者： Junli liu Wei Zhao Aixia Zhang pengliang Li Jingke liu Institute of Biotechnology and Food Science Hebei Academy of Agriculture and Forestry SciencesShijiazhuang 050000China

Sourdough flavors were closely related to microbial *** microbial diversity of foxtail millet dough during fermentation has never been ***,the metabolic potential and diversity of the bactenial community were analyzed by metagenomic during dough *** was the dominant phylum in the dough,within heterofementative lactic acid bacteria(***,Limosilactobacillus,Pediococcus and Lactobacillus)as the most abundant *** was gradually inhibited after ***_crustorum was notably found abundant during dough ***_fermentum increased markedly during fermentation,while Companilactobacillus_cnustorum decreased *** futher exploration,genes associated with metabolism were annotated through ***,Companilactobacillus and Pediococcus were actively engaged in glycolysis(ko00010),starch and sucrose metabolism(ko00500),and pyruvate metabolism(ko00620),leading in part to 1actic and acetic acid acumulations and dough *** and Lactiplantibacillus were the main contributors to key aninopeptidases ox/and transaminases involved in amino acid metabolism,which was nesponsible for flavor metabolite *** study will provide an enhanced understanding of the predominance and diversity of dough bactenial communities,and contribute to future strain screening in the dough for better flavor.

关键词： Sourdough Foxtail millet Metagenomics Gilycolysis Amino acid biosynthesis

Inhibition of spore germination of Ulva pertusa by the marine bacterium Pseudoalteromonas haloplanktis CI4

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Acta Oceanologica Sinica 2010年第1期29卷 69-78页

作者： MA Yuexin liu pengliang ZHANG Yongsheng CAO Shanmao LI Dantong CHEN Wei Key Laboratory of Mariculture Ministry of Agriculture Dalian Fisheries University Dalian116023 China

The effect of the bacterial strain C14 on the germination of spores from the green alga Ulva pertusa was assayed and it was found that the bacterial biofilm and cell-free supernatant strongly inhibited spore germination. In attempts to define the chemical nature of she antifouling substance in the supernatant of C14, the culture supernatants were tested for activity after heat treatment, enzymatic treatments, size fractionation, and separation into aqueous and organic fractions. Results suggest that this bacterium produces an extracellular component with specific activity toward algal spores that was heat-sensitive and between 3 and 10 kDa in molecular size. The exposure of the organic phase fraction to spores showed inhibitive effect on spore germination. Pronase and carboxypeptidase y did not significantly affect the activity of inhibitory component, suggesting that the component was not a protein or a peptide. The bacterium C14 was identified as Pseudoalteromonas haloplanktis based on the phenotypic characters and 16S rRNA gene analvsis.

关键词： Pseudoaltcromonas antifouling bacterium Ulva pertusa biofouling anti-algal activity

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充填步距对地表移动变形特征的控制影响

中国矿业大学学报 2022年第4期51卷 642-650页

作者：贾林刚张华兴刘鹏亮中煤科工生态环境科技有限公司北京100013 天地科技股份有限公司生态科技事业部北京100013 天地科技股份有限公司开采设计事业部北京100013

为了研究充填开采步距对覆岩移动及地表建筑物变形的影响,首先,采用弹性地基梁理论,建立了顶板的位移方程,并采用力学等效转换,推导得出了顶板最大跨距、控顶距与充填步距间的数学关系式,进而得到充填步距的理论计算公式;其次,根据地表... 详细信息

为了研究充填开采步距对覆岩移动及地表建筑物变形的影响,首先,采用弹性地基梁理论,建立了顶板的位移方程,并采用力学等效转换,推导得出了顶板最大跨距、控顶距与充填步距间的数学关系式,进而得到充填步距的理论计算公式;其次,根据地表建筑物的特性及对变形形式的敏感分析,得到建筑物的抗变形限值,并进一步计算得出地表沉陷的最大允许值,反算出顶板位移值,按照推导公式计算分析,得出顶板跨距及相应的充填步距.从而建立了充填步距与地表建筑物变形之间的相互关系,通过调整充填步距,达到控制顶板位移和地表沉陷以及保护建筑物的目的.通过实例应用分析,在设定地表水平变形为1 mm/m的变形限值时,得出充填步距为6.4 m,当井下充填步距设为该值时,实测地表下沉为237 mm,且水平变形值小于预设限值,达到了良好地控制地表移动变形及保护地面建筑物的效果.

关键词：充填开采充填步距地表变形沉陷控制顶板移动

压水堆燃料辐照后燃料棒棒间距数据机器视觉测量方法研究

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核动力工程 2022年第4期43卷 53-59页

作者：李伟才刘鹏亮王从政郭严中广核研究院有限公司广东深圳518031 中国科学院光电技术研究所成都610209

针对辐照后燃料棒棒间距数据获取和处理困难的问题,基于燃料棒几何特性及其在压水堆燃料组件中的排列方式,本文提出一种基于机器视觉的高效、可靠的燃料棒棒间距数据测量方法。该方法首先采用Retinex算法对水下燃料棒的采集图像进行增... 详细信息

针对辐照后燃料棒棒间距数据获取和处理困难的问题,基于燃料棒几何特性及其在压水堆燃料组件中的排列方式,本文提出一种基于机器视觉的高效、可靠的燃料棒棒间距数据测量方法。该方法首先采用Retinex算法对水下燃料棒的采集图像进行增强预处理;然后,针对燃料棒阵列的前后成像干扰问题,采取边缘增强和逐行灰度特征处理方法实现待测燃料棒与背景燃料棒的有效分离,并进一步提升图像清晰度;最后,对燃料棒图像的单行灰度值进行二次曲线拟合,获得各个燃料棒的亚像素边缘点坐标。乏燃料组件的现场实验验证结果表明,该方法可一次性实现16个燃料棒棒间距测量,且测量精度达±0.32 mm,可为燃料性能分析提供高效、可靠的数据支持。

关键词：压水堆燃料棒棒间距机器视觉测量

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连采连充膏体充填技术在郭家湾煤矿应用研究

煤炭技术 2023年第5期42卷 18-22页

作者：张增誉高超刘鹏亮国能榆林能源有限责任公司陕西榆林719000 天地科技股份有限公司北京100013

针对郭家湾煤矿地面建筑物下压煤资源多,采用传统地面搬迁方式开采成本高、难度大问题,结合矿井实际情况提出了连采连充膏体充填技术,阐述了该充填开采技术的技术原理及工艺流程,并采用概率积分法对充填开采区域对应的地表建(构)筑物地... 详细信息

针对郭家湾煤矿地面建筑物下压煤资源多,采用传统地面搬迁方式开采成本高、难度大问题,结合矿井实际情况提出了连采连充膏体充填技术,阐述了该充填开采技术的技术原理及工艺流程,并采用概率积分法对充填开采区域对应的地表建(构)筑物地表移动变形进行了预测,同时在郭家湾煤矿进行了工程应用。结果表明,工作面支巷最大顶板下沉量为278 mm,顶板围岩变形量较小,工作面开采过程中矿压显现较为平缓,无较大的动压显现现象。开采范围内对应的最大地表下沉量为9.2 mm,满足规定标准对地表建筑物的使用要求。

关键词： “三下”压煤连采连充膏体充填概率积分法移动变形极值

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松软破碎顶板回采巷道支护技术优化

山西能源学院学报 2022年第1期35卷 7-8,11页

作者：刘鹏亮西山煤电西铭矿山西太原030052

文章针对屯兰矿23011工作面松软破碎顶板巷道围岩变形大、现支护方式控制较差的现状,基于工作面顶板岩层的赋存条件及现支护方案,采用FLAC3D数值软件确定了"锚杆索+钢筋网"联合支护技术及其支护参数。现场监测结果表明:采用该联合支护... 详细信息

文章针对屯兰矿23011工作面松软破碎顶板巷道围岩变形大、现支护方式控制较差的现状,基于工作面顶板岩层的赋存条件及现支护方案,采用FLAC3D数值软件确定了"锚杆索+钢筋网"联合支护技术及其支护参数。现场监测结果表明:采用该联合支护技术后,巷道顶板变形最大值为35mm,实现了对松软破碎顶板变形的有效控制,保证了矿井的安全高效生产。

关键词：破碎顶板变形大联合支护现场监测

高应力煤体小煤柱护巷围岩稳定性控制技术研究

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煤炭工程 2023年第12期55卷 71-77页

作者：于健浩刘鹏亮崔锋中煤科工开采研究院有限公司北京100014 天地科技股份有限公司开采设计事业部北京100013

高应力环境临空小煤柱巷道面临围岩稳定性差,巷道维护难度大等问题,以福达煤矿15#煤层5 m小煤柱应用现场为背景,针对影响小煤柱巷道围岩稳定性的关键因素,包括:地应力水平高、围岩稳定性差、煤柱内部遗留钻场空区等,提出了侧向水力压裂... 详细信息

高应力环境临空小煤柱巷道面临围岩稳定性差,巷道维护难度大等问题,以福达煤矿15#煤层5 m小煤柱应用现场为背景,针对影响小煤柱巷道围岩稳定性的关键因素,包括:地应力水平高、围岩稳定性差、煤柱内部遗留钻场空区等,提出了侧向水力压裂卸压、临空巷道围岩稳定性控制及煤柱内隐蔽钻场高水材料充填技术方案,并开展现场应用,结果表明:(1)水力压裂关键层位起裂压力峰值为26.5 MPa,各孔最大压力均值22.3 MPa,孔内原生裂隙受高压水挤压作用逐渐张开,对顶板完整性的弱化作用显著;(2)基于巷道矿压数据分析结论,顶板锚杆应力峰值为108 kN,帮锚杆应力峰值为86 kN,均远低于锚杆的屈服载荷,未出现断锚现象。巷道两帮及顶板下沉量均在可控范围内,巷道收缩量较小,围岩整体稳定性得到有效控制;(3)隐蔽钻场充填高水材料总体积682.9 m3,高于钻场空区总体积,每个充填钻孔均保证返浆后停止注浆,钻场充满率较高,残留1.5 m煤柱稳定性较好,达到了预期效果。

关键词：高应力煤体小煤柱护巷水力压裂围岩稳定性钻场充填