Inhibition of spore germination of Ulva pertusa by the marine bacterium Pseudoalteromonas haloplanktis CI4

作     者：MA Yuexin LIU Pengliang ZHANG Yongsheng CAO Shanmao LI Dantong CHEN Wei MA Yuexin 1 ,LIU Pengliang 1,ZHANG Yongsheng 1,CAO Shanmao 1,LI Dantong 1,CHEN Wei 1 1 Key Laboratory of Mariculture,Ministry of Agriculture,Dalian Fisheries University,Dalian 116023,China

作者机构：Key Laboratory of Mariculture Ministry of Agriculture Dalian Fisheries University Dalian116023 China 

出 版 物：《Acta Oceanologica Sinica》 (海洋学报（英文版）)

年 卷 期：2010年第29卷第1期

页      面：69-78页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 071005[理学-微生物学] 090402[农学-农业昆虫与害虫防治] 10[医学] 

基　　金：The Natural Science Foundation of Liaoning Province under contract No. 20062129 

主　　题：Pseudoaltcromonas antifouling bacterium Ulva pertusa biofouling anti-algal activity 

摘      要：The effect of the bacterial strain C14 on the germination of spores from the green alga Ulva pertusa was assayed and it was found that the bacterial biofilm and cell-free supernatant strongly inhibited spore germination. In attempts to define the chemical nature of she antifouling substance in the supernatant of C14, the culture supernatants were tested for activity after heat treatment, enzymatic treatments, size fractionation, and separation into aqueous and organic fractions. Results suggest that this bacterium produces an extracellular component with specific activity toward algal spores that was heat-sensitive and between 3 and 10 kDa in molecular size. The exposure of the organic phase fraction to spores showed inhibitive effect on spore germination. Pronase and carboxypeptidase y did not significantly affect the activity of inhibitory component, suggesting that the component was not a protein or a peptide. The bacterium C14 was identified as Pseudoalteromonas haloplanktis based on the phenotypic characters and 16S rRNA gene analvsis.