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Horticultural Plant Journal 2024年第1期10卷 145-155页

作者： Xuesong Liu Ronghui An Guofeng Li Shufen Luo Huali Hu pengxia Li Institute of Agricultural Facilities and Equipment Jiangsu Academy of Agricultural SciencesNanjingJiangsu 210014China School of Food and Biological Engineering Jiangsu UniversityZhenjiangJiangsu 212013China Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement NanjingJiangsu 210014China Key Laboratory of Cold Chain Logistics Technology for Agro-Products Ministry of Agriculture and Rural AffairsNanjingJiangsu 210014China Jinan Fruit Research Institute All China Federation of Supply and Marketing CooperativesJinanShandong 250220China

Melatonin(MT)is a low molecular weight compound with multiple biological functions in plants.It is known to delay leaf senescence in various species.However,no data are available on the MT signaling pathway in postharvest vegetables.This study demonstrates that MT increases cGMP concentration and the expression of the cGMP synthesis gene BcGC1 in pak choi.The c GMP inhibitor LY83583 destroys effect of MT delaying the leaf senescence.LY83583 also prevents MT treatment from reducing the expression of chlorophyll metabolism-related genes(BcNYC1,BcNOL,BcPPH1/2,BcSGR1/2,and BcPAO)and senescence genes(BcSAG12 and BcSAG21).It also inhibits MT from reducing the activity of the key chlorophyll catabolism enzymes Mg-dechelatase,pheophytinase,and pheide a oxygenase.Thus,the ability of MT to maintain high levels of chlorophyll metabolites is also destroyed.The Arabidopsis c GMP synthetic gene mutant atgc1 was used to confirm that delayed leaf senescence caused by MT is mediated,at least in part,by the second messenger c GMP.

关键词： Pak choi Melatonin cGMP Chlorophyll degradation Senescence

Accelerated reconstruction of ZIF-67 with significantly enhanced glucose detection sensitivity

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Nano Research 2024年第6期17卷 4737-4743页

作者： Huihui Jin Weihao Zeng Wei Qian Lun Li pengxia Ji Zhengying Li Daping He School of Information Engineering Wuhan University of TechnologyWuhan 430070China Hubei Engineering Research Center of RF-Microwave Technology and Application Wuhan University of TechnologyWuhan 430070China State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of TechnologyWuhan 430070China

Research on metal-organic framework(MOF)-based non-enzymatic glucose sensors usually ignores the impact of the surface reconstruction degree of MOF on the activity of catalyzing glucose oxidation.In this work,we choose zeolitic imidazolate framework-67(ZIF-67),which is commonly used in glucose sensing,as a representative to investigate the influence of reconstruction degree on its structure and glucose catalytic performance.By employing the electrochemical activation strategy,the activity of ZIF-67 in catalyzing glucose gradually increased with the prolongation of the activation time,reaching the optimum after 2 h activation.The detection sensitivity of the activated ZIF-67 was 19 times higher than that of the initial ZIF-67,and the limit of detection(LOD)was lowered from 7 to 0.4μM.Our findings demonstrate that the oxidation degree of ZIF-67 deepened rapidly with continuously activation and was basically reconstructed to CoOOH after 2 h activation,accompanied by a morphological change from cuboctahedral to flower-like.Simultaneously,theoretical investigation revealed that ZIF-67 is not suitable as a stable glucose sensor electrode since the adsorbed glucose molecules hasten the dissociation of ligands and the breaking of Co-N bond in ZIF-67.Therefore,our work has important implications for the rational design of next-generation MOF-based glucose sensors.

关键词： zeolitic imidazolate framework-67(ZIF-67) glucose oxidation electrochemical activation reconstruction CoOOH

Hybrid ionic/electronic interphase enabling uniform nucleation and fast diffusion kinetics for stable lithium metal anode

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Chinese Chemical Letters 2024年第2期35卷 626-633页

作者： Lun Li pengxia Ji Meng Huang Zixin Zhang Hong Wang Francis Verpoort Jinlong Yang Daping He State Key Laboratory of Advanced Technology for Materials Synthesis and Processing School of Materials Science and EngineeringWuhan University of TechnologyWuhan 430070China Guangdong Research Center for Interfacial Engineering of Functional Materials College of Materials Science and EngineeringShenzhen UniversityShenzhen 518060China Sanya Science and Education Innovation Park of Wuhan University of Technology Sanya 572000China Hubei Engineering Research Center of RF-Microwave Technology and Application Wuhan University of TechnologyWuhan 430070China State Key Laboratory of Silicate Materials for Architectures Wuhan University of TechnologyWuhan 430070China

Lithium(Li)dendrite issue,which is usually caused by inhomogeneous Li nucleation and fragile solid electrolyte interphase(SEI),impedes the further development of high-energy Li metal batteries.However,the integrated construction of a high-stable SEI layer that can regulate uniform nucleation and facilitate fast Li-ion diffusion kinetics for Li metal anode still falls short.Herein,we designed an artificial SEI with hybrid ionic/electronic interphase to regulate Li deposition by in-situ constructing metal Co clusters embedded in LiF matrix.The generated Co and LiF both enable fast Li-ion diffusion kinetics,meanwhile,the lithiophilic properties of Co clusters can serve as Li-ion nucleation sites,thereby contributing to uniform Li nucleation and non-dendritic growth.As a result,a dendrite-free Li deposition with a low overpotential(16.1 mV)is achieved,which enables an extended lifespan over 750 h under strict conditions.The full cells with high-mass-loading LiFePO_(4)(11.5 mg/cm^(2))as cathodes exhibit a remarkable rate capacity of 84.1 mAh/g at 5 C and an improved cycling performance with a capacity retention of 96.4%after undergoing 180 cycles.

关键词： Lithium metal anode Hybrid ionic/electronic interphase Solid electrolyte interphase Fast diffusion kinetics Dendritic growth of lithium

内体分选转运复合体:确保有丝分裂正确进行的分子机器

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中国医学科学院学报 2024年第2期46卷 287-292页

作者：高吴陆怡杨玉张鹏霞商宇佳木斯大学基础医学院微生态-免疫调节网络与相关疾病重点实验室黑龙江佳木斯154007

有丝分裂是真核细胞增殖的重要环节。内体分选转运复合体是与膜剪切过程密切相关的多蛋白复合体,其功能涉及内体成熟、病毒出芽、自噬等过程,其结构和功能异常与癌症等疾病发生发展密切相关。本文总结了内体分选转运复合体在细胞有丝分... 详细信息

有丝分裂是真核细胞增殖的重要环节。内体分选转运复合体是与膜剪切过程密切相关的多蛋白复合体,其功能涉及内体成熟、病毒出芽、自噬等过程,其结构和功能异常与癌症等疾病发生发展密切相关。本文总结了内体分选转运复合体在细胞有丝分裂不同阶段所发挥的作用,以及内体分选转运复合体调控有丝分裂作用在疾病方面的研究进展。

关键词：有丝分裂内体分选转运复合体纺锤体检查点癌症

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基于杜邦分析体系的天润乳业盈利能力分析

中国乳业 2024年第1期 17-21,27页

作者：米鹏霞兰州财经大学甘肃兰州730101

新疆天润乳业股份有限公司(简称“天润乳业”)作为新疆龙头乳品企业,要想在全国市场赢得有利地位,需提高盈利能力。本文结合杜邦分析体系对天润乳业2018—2022年的5年财务数据进行分析,并针对其盈利能力存在的问题提出建议,为其他乳品... 详细信息

新疆天润乳业股份有限公司(简称“天润乳业”)作为新疆龙头乳品企业,要想在全国市场赢得有利地位,需提高盈利能力。本文结合杜邦分析体系对天润乳业2018—2022年的5年财务数据进行分析,并针对其盈利能力存在的问题提出建议,为其他乳品企业提供参考。

关键词：杜邦分析盈利能力天润乳业

Spatiotemporal variations in the organic carbon accumulation rate in mangrove sediments from the Yingluo Bay,China,since 1900

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Acta Oceanologica Sinica 2021年第8期40卷 65-77页

作者： Yao Zhang Xianwei Meng pengxia Jun Zhang Dahai Liu Zhen Li Wanzhu Wang College of Marine Geosciences Ocean University of ChinaQingdao 266003China First Institute of Oceanography Ministry of Natural ResourcesQingdao 266061China Laboratory for Marine Geology and Environment Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266061China School of Earth and Ocean Sciences University of VictoriaBritish Columbia V8W2Y2Canada

Mangroves can not only provide multiple ecosystem service functions,but are also efficient carbon producers,capturers,and sinks.The estimation of the organic carbon accumulation rate(OCAR)in mangrove sediments is fundamental for elucidating the role of mangroves in the global carbon budget.In particular,understanding the past changes in the OCAR in mangrove sediments is vital for predicting the future role of mangroves in the rapidly changing environment.In this study,three dated sediment cores from interior and fringe of mangroves in the Yingluo Bay,China,were used to reconstruct the spatiotemporal variations of the calculated OCAR since 1900 in this area.The increasing OCAR in the mangrove interior was attributed to mangrove flourishment induced by climate change characterized by the rising temperature.However,in the mangrove fringe,the strengthening hydrodynamic conditions under the sea level rise were responsible for the decreasing OCAR,particularly after the1940 s.Furthermore,the duration of inundation by seawater was the primary factors controlling the spatial variability of the OCAR from the mangrove fringe to interior,while the strengthened hydrodynamic conditions after the 1940 s broke this original pattern.

关键词： spatiotemporal variation organic carbon accumulation rate organic carbon source mangrove development temperature hydrologic conditions

Aurora A orchestrates entosis by regulating a dynamic MCAK-TIP150 interaction

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Journal of Molecular Cell Biology 2014年第3期8卷 240-254页

作者： pengxia JinhuaZhou Xiaoyu Song BingWu XingLiu Di Li Shuyuan Zhang Zhikai Wang H uijuan Yu Tarsha Ward Jiancun Zhang Yinmei Li Xiaoning Wang YongChen Zhen Guo Xuebiao Yao Anhui Key Laboratory of Cellular Dynamics & Chemical Biology Department of Optics and Optical Engineering and Hefei National Laboratory for Physical Sciences at Nanoscale University of Science and Technology of China Hefei 230027 China Molecular Imaging Center Atlanta Clinical and Translational Science Institute Morehouse School of Medicine Atlanta GA 30310 USA Harvard Medical School Boston MA 02115 USA Guangzhou Institutes of Biomedicine and Health Guangzhou 510513 China The 301 Hospital Beijing 100039 China Xijing Hospital The Fourth Military Medical University Xi'an 710032 China

Entosis, a cell-in-cell process, has been implicated in the formation of aneuploidy associated with an aberrant cell division control. Microtubule plus-end-tracking protein TIP150 facilitates the loading of MCAK onto the microtubule plus ends and orchestrates microtubule plus-end dynamics during cell division. Here we show that TIP150 cooperates with MCAK to govern entosis via a regulatory circuitry that involves Aurora A-mediated phosphorylation of MCAK. Our biochemical analyses show that MCAK forms an intra-molecular association, which is essential for TIP150 binding. Interestingly, Aurora A-mediated phosphorylation of MCAK modulates its intra-molecular association, which perturbs the MCAK-TIP150 interaction in vitro and inhibits entosis in vivo. To probe if MCAK-TIP150 interaction regulates microtubule plasticity to affect the mechanical properties of cells during entosis, we used an optical trap to measure the mechanical rigidity of live MCF7 cells. We find that the MCAK cooperates with TIP150 to promote microtubule dynamics and modulate the mechanical rigidity of the cells during entosis. Our results show that a dynamic interaction of MCAK-TIP150 orchestrated by Aurora A-mediated phosphorylation governs entosis via regulating microtubule plus-end dynamics and cell rigidity. These data reveal a previously unknown mechanism of Aurora A regulation in the control of microtubule plasticity during cell-in-cell processes.

关键词： s期极光编排微管动力学细胞分裂生物化学分析机械性能相互作用

PDCA循环管理在西药配药中的应用效果及对降低配药内部差错发生率的影响

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临床医学研究与实践 2024年第18期9卷 183-186页

作者：高鹏侠罗雅莉宝鸡市人民医院陕西宝鸡721000

目的探讨PDCA循环管理在西药配药中的应用效果及对降低配药内部差错发生率的影响。方法随机选择2020年2月至2022年2月我院开具的80张西药处方单,以非同周期法将其分为对照组和观察组,每组40张。对照组实施常规管理,观察组实施PDCA循环... 详细信息

目的探讨PDCA循环管理在西药配药中的应用效果及对降低配药内部差错发生率的影响。方法随机选择2020年2月至2022年2月我院开具的80张西药处方单,以非同周期法将其分为对照组和观察组,每组40张。对照组实施常规管理,观察组实施PDCA循环管理。管理前、后西药房共计12名工作人员。比较两组的配药差错发生情况、整体管理质量、配药用时、取药等候时间及满意度。结果观察组的配药差错总发生率低于对照组,差异具有统计学意义(P<0.05)。观察组的存放管理、制度落实、标志管理、培训评分高于对照组,差异具有统计学意义(P<0.05)。观察组的配药用时、取药等候时间短于对照组,差异具有统计学意义(P<0.05)。观察组的满意度高于对照组,差异具有统计学意义(P<0.05)。结论PDCA循环管理在西药配药中的应用效果明显,可降低配药差错发生率,缩短配药用时、取药等候时间,提升整体管理质量及患者满意度,值得临床进一步推广和应用。

关键词： PDCA循环管理西药配药配药差错管理质量工作效率

传统泡菜中高效降解核苷乳酸菌的筛选及其特性研究

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食品与发酵工业 2024年第12期50卷 227-233页

作者：李杰张鹏霞周健贺敏彭景贤包艳宋丽新佳木斯大学公共卫生学院黑龙江佳木斯154000 佳木斯大学基础医学院黑龙江佳木斯154000 包头市疾病预防控制中心内蒙古包头014030 内蒙古科技大学包头医学院公共卫生学院内蒙古包头014060

高尿酸血症是一种因尿酸代谢障碍而引发的一种代谢性疾病。乳酸菌作为一类重要的益生菌可通过多种方式改善高尿酸血症。该研究旨在从泡菜中筛选能够高效降解核苷的乳酸菌并分析其特性。利用高效液相色谱法分析所筛选菌株的核苷降解能力... 详细信息

高尿酸血症是一种因尿酸代谢障碍而引发的一种代谢性疾病。乳酸菌作为一类重要的益生菌可通过多种方式改善高尿酸血症。该研究旨在从泡菜中筛选能够高效降解核苷的乳酸菌并分析其特性。利用高效液相色谱法分析所筛选菌株的核苷降解能力,检测菌株对黄嘌呤氧化酶抑制作用、体外环境耐受性及抗生素敏感性。结果表明,短乳杆菌(Levilactobacillus brevis)SLlac-18对肌苷和鸟苷的降解率分别可达97.81%和97.89%;且对黄嘌呤氧化酶的活力具有一定抑制作用。此外,在pH 2.0的环境下,SLlac-18的存活率可达96%;在3 g/L胆盐环境下处理5 h条件下,SLlac-18的存活率可达96.33%;而在胃肠液环境中,SLlac-18同样表现较高的耐受性,存活率均在90%以上,且菌株对红霉素、氨苄西林等4种常见抗生素具有敏感性。短乳杆菌SLlac-18具有潜在降尿酸功能,且具有良好的益生特性,可以应用于高尿酸血症预防和辅助治疗中,具有一定应用价值。

关键词：高尿酸血症核苷乳酸菌泡菜益生菌

微酸性电解水对采后香葱抑菌特性及贮藏品质的影响

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食品科学 2024年第9期45卷 197-203页

作者：焦贺孟敌韩颖王馨渝李鹏霞胡花丽李国锋刘玲沈阳农业大学食品学院辽宁沈阳110866 江苏省农业科学院农业设施与装备研究所江苏南京210014 农业农村部农产品冷链物流技术重点实验室江苏南京210014

为探究微酸性电解水(slightly acidic electrolyzed water,SAEW)对香葱采后贮藏品质的影响,本实验分析低温((4±1)℃)贮藏条件下不同质量浓度(0、100、200 mg/L和300 mg/L)SAEW浸泡处理5 min后香葱采后抑菌特性及贮藏品质的变化。结果显... 详细信息

为探究微酸性电解水(slightly acidic electrolyzed water,SAEW)对香葱采后贮藏品质的影响,本实验分析低温((4±1)℃)贮藏条件下不同质量浓度(0、100、200 mg/L和300 mg/L)SAEW浸泡处理5 min后香葱采后抑菌特性及贮藏品质的变化。结果显示,较对照和其他质量浓度(100 mg/L和300 mg/L)SAEW处理相比,200 mg/L SAEW浸泡处理更好地维持了采后香葱的外观品质并抑制了其菌落总数的增长。进一步的研究结果显示,200 mg/L SAEW浸泡处理可有效抑制香葱中质量损失率和腐烂率的增加,减缓叶绿素、VC、可溶性糖以及可溶性蛋白含量的下降,减少有害物质总游离氨基酸、丙二醛和亚硝酸盐的积累。综上,200 mg/L SAEW浸泡处理不仅能有效抑制低温贮藏期间香葱中菌落总数的增长,而且可有效延缓该过程中香葱营养品质的流失,从而提高香葱的保鲜效果。因此,SAEW可作为一种维持采后香葱贮藏品质,延缓其衰老进程的有效杀菌、保鲜措施。

关键词：微酸性电解水香葱杀菌贮藏品质