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生理学报 1987年第1期39卷 1-9页

作者：鲁立潘国宗中国医学科学院北京协和医院内科中国医学科学院北京协和医院内科进修生北京医科大学生理教研室王志均教授的研究生

为探讨胰多肽抑制胰酶分泌的机制,我们利用大鼠离体胰腺泡制备观察了牛胰多肽（BPP）在细胞受体水平对氨甲酰胆碱等促分泌物作用的影响。实验结果显示,BPP 对氨甲酰胆碱诱导的胰腺泡淀粉酶分泌具有抑制作用,并存在剂量反应关系。BPP0.1... 详细信息

为探讨胰多肽抑制胰酶分泌的机制,我们利用大鼠离体胰腺泡制备观察了牛胰多肽（BPP）在细胞受体水平对氨甲酰胆碱等促分泌物作用的影响。实验结果显示,BPP 对氨甲酰胆碱诱导的胰腺泡淀粉酶分泌具有抑制作用,并存在剂量反应关系。BPP0.1μmol/L 和0.2μmol/L,可分别使氨甲酰胆碱诱导淀粉酶分泌的效价降低3倍和10倍;BPP 还可抑制氨甲酰胆碱刺激胰腺泡释放45Ca。以上结果提示,BPP 对胰腺泡的胆碱能 M 受体具有拮抗作用。此外,BPP 对促胰液素及其同类激动剂和氨甲酰胆碱协同作用诱导的胰腺泡淀粉酶分泌具有抑制作用,提示胰多肽在整体对促胰液素诱导的胰酶分泌的抑制,可能是通过拮抗胰腺泡细胞上的 M 受体而抑制了促胰液素和胆碱能刺激协同作用引起的胰酶分泌。

关键词：胰腺泡促胰液素血管活性肠肽胆囊收缩素淀粉酶分泌

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INHIBITORY ACTION OF （-） CLAUSENAMIDE ON HYPERPHOSPHORYLATION OF TAU PROTEIN IN EXPERIMENTAL DIABETIC MICE

INHIBITORY ACTION OF （-） CLAUSENAMIDE ON HYPERPHOSPHORYLAT...

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2004 National Meeting on Pharmacology of Young Pharmacologists

作者： Yong CHENG Jun-Tian ZHANG Department of Pharmacology, Institute of Material Medica, peking union medical college & chinese academy of medical sciences, Beijing 100050, China

＜正＞ AIMS: Neurofibrillary tangles （NFTS） are histopathological lesions in Alzheimer’s disease（AD）. The core component of PHFs, which make up NFTs, results from abnormalhyperphosphorylated of microtubule-associated protein tau. The microtubule stabilizingfunction of tau is greatly diminished by its hyperphosphorylation to PHF-tau, which bindspoorly to tubulin and leads to cytoskeletal loss and cell death. The diabetes mice which wereinduced by streptozotocin （STZ）, the insulin-secreting β-cell killer, appeared tau proteinhyperphosphorylation at Ser 199/202 sites in the central nervous system. （-） Clausenamide, anew compound isolated from clausena lausium （lour） skeel, which showed neuroprotectiveeffects in the central nervous system in previous studies. The Aim in present study is todetect the effects of （-） clausenamide on inhibiting tau protein hyperphosphorylation in theseexperimental diabetic ***: The mice were injected STZ at 200 mg/kg, once, i.p. The successful diabeticmodels were divided into model （DM） group, （-） clausenamide 7.5、15、30mg/kg groups, andthe mice untreated with STZ as control group. The Morris water maze and step-through testwere performed at 7 weeks after STZ was given. After behavior assays, mice were *** in neuron of CA1 of hippocampus were detected by electron microscope andimmunohistochemistry was performed with AT-8、tau-1、GSK-3、CDK-5、PP-1 and ***: The diabetic mice showed neuropathological lesions such as impaired ability oflearning and memory, hyperphosphorylation of tau protein at Ser199/202 sites, fragmentationand dissolution of microtubules, the expression of kinases （GSK-3 and CDK-5） increasingand phosphatase （PP-1） decreasing in hippocampal neuron. While in the mice which hadreceived （-） clausenamide, the microtubule structure showed morphology improvement at7.5-15mg/kg, morphology was almost normal at 30mg/kg. In immunohistochemistry fortubulin antibody, there was also gradual increase i

关键词： Alzheimer’s disease (-)clausenamide tau protein hyperphosphorylation microtubule

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THE TREATMENT OF ADVANCED NON-SMALL-CELL LUNG CANCER （NSCLC）-OVERSEAS UPDATED AND OUR EXPERIENCES

THE TREATMENT OF ADVANCED NON-SMALL-CELL LUNG CANCER （NSCLC...

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中国抗癌协会第六届临床肿瘤协作中心（CSCO）学术年会

作者： Datong Chu Xiangru Zhang Junling Li Fenglian Qu Yan Sun Cancer Institute and Hospital, peking union medical college chinese academy of medical sciences

＜正＞Introduction The article briefly reviewed the evolution of chemotherapy in the treatment of advanced stage non-small cell lung cancer （NSCLC） over the past 3 decades. After certain period of hesitation, it has become clear that the chemotherapy, and in particular, cisplatin-based regimens, when com pared with best supportive care （BSC）, provides a modest survival advantage in NSCLC. With modern chemotherapy, median survival time averages around 8 to 11 months and 1-year survival ranged 30%～45%, the response rate reaches 25%～35% and with improved quality of life. The

关键词： Non-small cell lung cancer Chemotherapy Advanced stage

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Synthesis of neotigogenyl glycosides using orthogonal protection-deprotection glycosylation

Synthesis of neotigogenyl glycosides using orthogonal protec...

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The 3～(rd) International Conference on Natural Products :Natural Products -a Must for Human Survival ABSTRACTS

作者： Shujie Hou, Chuanchun Zou, Pingsheng Lei, Dequan Yu Institute of Materia Medica, peking union medical college & chinese academy of medical sciences, Beijing 100050

In order to increase the novel structure-activity relationship available for tigogenyl glycosides, a group of new structurally tigogenyl saponins with glucosamine was designed and synthesized by utilization of orthogonal protection-deprotection *** 1 was copupled with tigogenin giving 2. Removal of the acetyl group from 2 afforded the triol 3. Treatment 4 with 80% acetic acid produced 5. Selective monosilyation of 5 then treatment with chloroacetic anhydride provided *** the key compound 6 in hand, attention was turned to examining the orthogonality of the three chosen protecting groups (TBDMS, ClAc, and Bn). 6 was treated with ammonium cerium (Ⅳ) nitrate leading to clean conversion from 6 to 7. Using thiourea, chloroacetyl group was removed off to provide 8 in high yield. At the condition of Pd/C under hydrogen, the building block 6 was hydrogenised giving 9. Taking compounds 7, 8, 9 as acceptors to couple with protected D-glucopyranosyl trichloroacetimidate 10 and protected L-rhmanopyranosyl trichloroacetimidate 11, a group of neotigogenyl glycosides with disaccharide and trisaccharide were synthesized in a facile way.

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Clinical significance of serum levels of thyroid stimulating hormone receptor antibody in pregnant women with Graves' disease

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生殖医学杂志 2010年第A2期19卷 49-53页

作者：赵志英田健朱立 Department of Nuclear Medicine Peking Union Medical College HospitalPeking Union Medical CollegeChinese Academy of Medical Sciences

Objective:To investigate the clinical significance of serum thyroid stimulating hormone(TSH) receptor antibody (TRAb) levels and the antithyroid drug(ATDs) use in pregnant women with Graves' disease in their neonatal thyroid function. Methods:The serum TRAb and T3,T4,FT3,FT4,TSH levels in 68 pregnant women with Graves' disease and their newborns were detected by radio receptor assay(RRA) and electrical chemiluminescence immunoassay (ECLIA),*** on the maternal serum TRAb levels and the use of antithyroid drugs during pregancy, the newborns were divided into different *** incidence of neonatal thyroid dysfunction and its risk factors were analyzed. Results:The results showed the incidence of abnormal thyroid function of newborns was 29.4%(20/68).The proportion of neonatal thyroid dysfunction in women with high TRAb levels in the third trimester of pregnancy were significantly higher than these with normal TRAb(P<0.01).In 23 newborns whose mothers were normal in serum TRAb levels and took no ATDs during pregnancy,only one case had thyroid dysfunction within two weeks after birth,while in other 45 newborns whose mothers had a high level of serum TRAb and/or took ATDs during pregnancy, 19 developed thyroid dysfunction within two weeks after birth. Conclusion:Neonatal thyroid function depends on the balance between the transplacental TRAb and ATDs. TRAb measurement in pregnant women with Graves' disease is of significance in evaluation of neonatal thyroid function. Elevated level of serum TRAb in the third trimester of pregnancy is a risk factor for neonatal thyroid dysfunction.

关键词：甲状腺激素受体抗体血清临床意义孕妇甲状腺功能化学发光免疫分析功能障碍新生儿

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链霉菌质粒pSGL1最小复制子序列测定及分析

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微生物学报 1999年第4期39卷 327-332页

作者：张华洪斌李元中国医学科学院协和医科大学医药生物技术研究所

质粒ｐＳＧＬ１（７．４ｋｂ）是从球孢链霉菌（Ｓｔｒｅｐｔｏｍｙｃｅｓｇｌｏｂｉｓｐｏｒｕｓ）中分离得到的一个高拷贝质粒，已证明其最小复制子位于Ｓａｕ３ＡＩ酶切的２０ｋｂ片段上。对该片段进行亚克隆，测序后数据表明该片... 详细信息

质粒ｐＳＧＬ１（７．４ｋｂ）是从球孢链霉菌（Ｓｔｒｅｐｔｏｍｙｃｅｓｇｌｏｂｉｓｐｏｒｕｓ）中分离得到的一个高拷贝质粒，已证明其最小复制子位于Ｓａｕ３ＡＩ酶切的２０ｋｂ片段上。对该片段进行亚克隆，测序后数据表明该片段是一个新序列。仅有一个开放阅读框架（ＯＲＦＲ）位于最小复制子中，推测其编码的蛋白质含有滚环复制质粒复制酶的特定序列。

关键词：质粒最小复制子滚环复制序列分析链霉菌

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The role of delayed rectifier potassium channel subtype in neuronal apoptosis

The role of delayed rectifier potassium channel subtype in n...

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2002全国青年药理学英文学术会议

作者： JIN Hong-Wei ZHANG Wei WANG Xiao-Liang(Institute of Materia Medico, peking union medical college & chinese academy of medical sciences, Beijing 100050, China)

AIM: Many evidence show that K efflux through potassium channels is an important phenomenon in neuronal apoptosis. We want to further elucidate the role of voltage-gated outward potassium channel subtype in neuronal apoptosis or necrosis. METHODS: (1) MTT assay, whole cell patch clamp recording and RT-PCR were performed to explore changes of cell viability, voltage-gated outward potassium channel currents and mRNA expression in primary cultured hippocampal neurons after exposure to beta-amyloid peptide (β-AP. (2) Flow cytometry was used to analysis the changes of cell size,

关键词： apoptosis delayed rectifying potassium channel Kv2.1

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UV radiation-mediated expression of inducible nitric oxide synthase in normal human keratinocytes and production of Leukotriene B4 5-LOX mRNA expression in Human Skin Fibroblasts

UV radiation-mediated expression of inducible nitric oxide s...

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中华医学会第十二次全国皮肤性病学术会议

作者： Yan Yan, Yang Min, Zuo Yagang, Qu Tao, Wang Baoxi peking union medical college Hospital,chinese academy of medical sciences, Beijing, China.

Purpose To investigate the expression of iNOS in normal human keratinocytes(NHK) by UVB irradiation and the production of leukotriene B4 and expression of 5- LOX mRNA in skin fibroblasts(HFB) induced by UVA irradiation. Methods NHK and HFB were cultured and irradiated with UVB and UVA. After UVB exposure, iNOS expression in NHK was measured by immunofluorescence technique and Western-blotting assay at 24h and 48h. After UVA irradiation, Enzyme-linked immunosorbent assay(ELIS A) was applied to detect the production of leukotriene B4 in the supernatant of cell culture and a reverse transcription polymerase chain reaction (RT-PCR) was used to detect

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Association between P2Y12 platelet receptor(C34T and G52T) polymorphisrns and risk of cardiovascular events in coronary heart disease with clopidogrel

Association between P2Y12 platelet receptor(C34T and G52T) p...

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第十三次全国心血管病学术会议

作者： TANG Xiao-fang,YUAN Jin-qing Cardiovascular Institute and Fu Wai Hospital,peking union medical college and chinese academy of medical sciences,Beijing 100037,China

Objective Recent datas have implicated a haplotype of P2Y12 platelet receptor,as potential risk determinant for atherothrombosis. We wanted to investigate whether the platelet P2Y12 receptor polymorphims a ected long-term prognosis of chinese patients who were treated with clopidogrel after percutaneous coronary intervention(PCl).Methods Between November 1,2008 and November 31,2009,268 Patients who received PCI and were exposed to clopidogrel treatment for almost 12 months,were enrolled in Fu Wai Hospital and underwent P2Y12(G52T and C34T) ***-up was 12

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The awareness of disease-related knowledge and demands of internet health education in patients with pulmonary arterial hypertension

The awareness of disease-related knowledge and demands of in...

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第十三次全国心血管病学术会议

作者： ZHANG Chen,LIANG Tao,CHEN Yan *Cardiovascular Institute and Fu Wai Hospital,peking union medical college and chinese academy of medical sciences,Beijing 100037,China

Objective To know the awareness of patients with pulmonary arterial hypertension on disease-related knowledge,especially on treatment and general knowledge of life-style;to research the internet-using actuality of patients,to know their demands with internet health *** A questionnaire survey to 90 pulmonary arterial hypertension patients was *** 12.2%patients have a good level of disease-related knowledge,50.0%patients have a basic level,and

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