The role of delayed rectifier potassium channel subtype in neuronal apoptosis

作     者：JIN Hong-Wei ZHANG Wei WANG Xiao-Liang(Institute of Materia Medico, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100050, China) 

会议名称：《2002全国青年药理学英文学术会议》

会议日期：2002年

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100104[医学-病理学与病理生理学] 10[医学] 

关 键 词：apoptosis delayed rectifying potassium channel Kv2.1 

摘      要：AIM: Many evidence show that K efflux through potassium channels is an important phenomenon in neuronal apoptosis. We want to further elucidate the role of voltage-gated outward potassium channel subtype in neuronal apoptosis or necrosis. METHODS: (1) MTT assay, whole cell patch clamp recording and RT-PCR were performed to explore changes of cell viability, voltage-gated outward potassium channel currents and mRNA expression in primary cultured hippocampal neurons after exposure to beta-amyloid peptide (β-AP. (2) Flow cytometry was used to analysis the changes of cell size,