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中华预防医学杂志 2024年第5期58卷 711-718页

作者：胡海圣罗文婷郑佩燕孙宝清广州医科大学附属第一医院检验科呼吸疾病全国重点实验室国家呼吸医学中心国家呼吸系统疾病临床研究中心广州呼吸健康研究院广州510120

人体作为一个高度复杂的生态系统,生长着多种多样的微生物群落,其中引发过敏反应的主要为皮肤微生物组和真菌。全球真菌的种类数量大致介于60万至100万之间,在理论上,IgE介导的致敏作用可以由任何真菌物种导致。但截至目前,世界卫生组织... 详细信息

人体作为一个高度复杂的生态系统,生长着多种多样的微生物群落,其中引发过敏反应的主要为皮肤微生物组和真菌。全球真菌的种类数量大致介于60万至100万之间,在理论上,IgE介导的致敏作用可以由任何真菌物种导致。但截至目前,世界卫生组织/国际免疫学联合会(IUIS)官方数据库中只记录了113种真菌过敏原,这些过敏原源自30种不同的真菌,涵盖了42个过敏原家族。而对于皮肤微生物组,已经鉴定出14种不同的马拉色菌过敏原,这些过敏原均源自3种不同的马拉色霉菌,即毛滴虫、分枝杆菌和***。这些过敏病患者的过敏原组分致敏谱异常复杂。本文将详细阐释欧洲过敏和临床免疫学会(EAACI)发布的《过敏原组分诊断指导建议2.0》中关于皮肤微生物组和真菌过敏的最新研究进展和临床应用。同时,整理了国内外真菌过敏原的来源、过敏原组分蛋白家族的特征、临床相关性以及诊断策略,以提升相关专业人员对过敏原组分的深刻理解。通过运用先进的过敏原组分诊断技术,旨在精准诊疗过敏患者,为研究中国人群的皮肤微生物组和真菌过敏原蛋白致敏谱奠定基础。

关键词：霉菌微生物过敏

皮下特异性免疫治疗早期对尘螨变应原特异性抗体及多元不饱和脂肪酸代谢水平的影响分析

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中华预防医学杂志 2024年第6期58卷 778-790页

作者：李诗韵郑佩燕翟莺莺张毅达甘辉黄惠敏罗文婷蒋满云张桂珍钟瑞芬程章恺孙世学孙宝清广州医科大学附属第一医院临床检验中心呼吸疾病全国重点实验室国家呼吸医学中心国家呼吸系统疾病临床研究中心广州呼吸健康研究院广州510120 广州医科大学附属第一医院儿科广州510120 东莞市第八人民医院检验科东莞523003 广州医科大学药学院广州511436

目的探讨过敏原皮下特异性免疫治疗(subcutaneous immunotherapy,SCIT)在治疗早期对患者免疫指标和代谢水平的影响,深入了解SCIT治疗在调节免疫反应和代谢水平方面的作用,为进一步发现潜在生物标志物提供参考数据。方法采用纵向研究方法... 详细信息

目的探讨过敏原皮下特异性免疫治疗(subcutaneous immunotherapy,SCIT)在治疗早期对患者免疫指标和代谢水平的影响,深入了解SCIT治疗在调节免疫反应和代谢水平方面的作用,为进一步发现潜在生物标志物提供参考数据。方法采用纵向研究方法,纳入2017年11月至2022年2月期间于广州医科大学附属第一医院儿科接受尘螨过敏原SCIT的受试者40例,其中单螨制剂治疗(single mite subcutaneous immunotherapy,SM-SCIT)和双螨制剂(double mite subcutaneous immunotherapy,DM-SCIT)治疗受试者各20例。本研究检测了受试者治疗前和治疗12个月后的尘螨过敏原特异性抗体和多元不饱和脂肪酸代谢水平,同时进行了肺功能检查,并通过症状视觉模拟量表(visual analogue scale,VAS)、哮喘控制测试问卷(asthma control test,ACT)和用药总评分(total medication scores,TMS)对患者的疗效进行随访,采用t检验、Mann-Whitney U检验等方法进行结果统计分析。结果经SCIT治疗12个月后,两组的VAS总分(SM-SCIT:Z=-2.298,P0.05),Der p、Der f、p 1、p 2、f 2和p 21组分sIgG4水平均显著升高(Z分别为-2.651、-3.771、-2.949、-2.912、-2.725、-2.128、-3.285,P均<0.05);双螨治疗组的Der p 2、f 2、p 7和p 23组分sIgE(Z分别为-1.965、-2.028、-2.406、-2.134,P均<0.05)和Der p、Der f、p 1、p 2、f 1、f 2、p 10、p 21和p 23组分sIgG4水平均显著升高(Z分别为-3.808、-3.845、-3.061、-2.688、-2.464、-3.211、-2.371、-2.091、-2.427,P均<0.05)。代谢组学分析显示,在治疗初期,SM-SCIT治疗后花生四烯酸、二十二碳六烯酸、二十二碳五烯酸、二十碳五烯酸、5,9,12-十八碳三烯酸、5(S)-羟化二十烷四烯酸、二高-γ-亚麻酸显著升高(Z分别为-2.191、-2.497、-1.988、-2.090、-2.19、-2.803、-2.073,P均<0.05);DM-SCIT组治疗后5(S)-羟化二十烷四烯酸显示升高,α-亚麻酸、二十碳二烯酸、二十碳一烯酸显著下降(Z分别-1.988、-2.090、-2.497、-1.988,P均<0.05)。相关性分析表明,花生四烯酸与尘螨特异性IgG4的变化呈显著负相关(r=-0.499,P<0.05),α-亚麻酸、5,9,12-十八碳三烯酸、二十碳五烯酸与尘螨der p 2的ΔsIgG4(r分别为0.451、0.420、0.474,P均<0.05)呈正相关。结论SCIT期间过敏原特异性抗体水平和多不饱和脂肪酸代谢水平发生显著变化,并且两者之间可能相互影响、相互作用。

关键词：过敏性哮喘尘螨组分代谢组学羟基二十碳四烯酸皮下特异性免疫治疗

High salt diet accelerates the progression of murine lupus through dendritic cells via the p38 MAPK and STAT1 signaling pathways

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Signal Transduction and Targeted Therapy 2020年第1期5卷 2082-2094页

作者： Ze Xiu Xiao Xiaojiang Hu Ximei Zhang Zhigang chen Julie Wang Ke Jin Feng lin Cao baoqing sun Joseph A.Bellanti Nancy Olsen Song Guo zheng Department of Clinical Immunology The Third Affiliated Hospital at Sun Yat-sen University510630 GuangzhouChina Department of Internal Medicine Ohio State University College of Medicine and Wexner Medical CenterColumbusOH 43210USA Department of Medicine Penn State College of MedicineHersheyPA 17033USA Laboratory of Human Diseases and Immunotherapy West China HospitalSichuan UniversityChengduChina Department of Internal Medicine The First Hospital of Harbin Medical UniversityHarbinChina Department of Allergy and Clinical Immunology The First Affiliated Hospital of Guangzhou Medical UniversityGuangzhouChina Department of Pediatrics and Microbiology-Immunology Georgetown University Medical CenterWashingtonDCUSA

The increased incidence of systemic lupus erythematosus(SLE)in recent decades might be related to changes in modern dietary *** sodium chloride(NaCl)promotes pathogenic T cell responses,we hypothesize that excessive salt intake contributes to the increased incidence of autoimmune diseases,including *** the importance of dendritic cells(DCs)in the pathogenesis of SLE,we explored the influence of an excessive sodium chloride diet on DCs in a murine SLE *** used an induced lupus model in which bone marrow-derived dendritic cells(BMDCs)were incubated with activated lymphocyte-derived DNA(ALD-DNA)and transferred into C57BL/6 recipient *** observed that a high-salt diet(HSD)markedly exacerbated lupus progression,which was accompanied by increased DC *** treatment also stimulated the maturation,activation and antigenpresenting ability of DCs in *** of BMDCs with NaCl also exacerbated BMDC-ALD-DNA-induced *** mice had increased production of autoantibodies and proinflammatory cytokines,more pronounced splenomegaly and lymphadenopathy,and enhanced pathological renal *** p38 MAPK–STAT1 pathway played an important role in NaClinduced DC immune *** together,our results demonstrate that HSD intake promotes immune activation of DCs through the p38 MAPK–STAT1 signaling pathway and exacerbates the features of ***,changes in diet may provide a novel strategy for the prevention or amelioration of lupus or other autoimmune diseases.

关键词： STAT1 lupus prevention

多变应原致敏呼吸道变应性疾病患者的112种变应原组分分布

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中华检验医学杂志 2015年第6期38卷 412-415页

作者：罗文婷韦妮莉郑佩燕黄惠敏孙宝清广州医科大学附属第一医院变态反应科广州呼吸疾病研究所呼吸疾病国家重点实验室 510120

目的：初步探讨分子变应原诊断在呼吸道变应性疾病中的作用，分析变应原分子图谱。方法回顾性收集2013年1月至2014年12月广州医科大学附属第一医院的多变应原致敏的哮喘和（或）过敏性鼻炎的患者血清，采用变应原组分蛋白芯片检测患者... 详细信息

目的：初步探讨分子变应原诊断在呼吸道变应性疾病中的作用，分析变应原分子图谱。方法回顾性收集2013年1月至2014年12月广州医科大学附属第一医院的多变应原致敏的哮喘和（或）过敏性鼻炎的患者血清，采用变应原组分蛋白芯片检测患者血清中112个变应原组分特异性IgE。结果78.8％（63／80）患者是3种或3种变应原组分致敏，检出阳性率最高的是Der f 167.5％（54／80），Der f 266.2％（53／80），Der p 1和Der p 2为63.8％（51／80）和61.2％（49／80），Der p 10仅占12.5％（10／80），其次为Fel d 122.5％（18／80）、Cyn d 116.2％（13／80）和Phl p 415.0％（12／80），Pen m 1、Pla a 2、Jug r 2和Can f 1均为11.2％（9／80），Lep d 2、Blo t 5、Bla g 7和Ani s 310.0％（8／80）。其他变应原组分阳性率比较低。结论分子变应原诊断可为过敏患者提供过敏图谱，提供更全面的诊断信息，发现经常被忽视的花粉变应原如百慕达草、梯牧草和梧桐树等。（中华检验医学杂志，2015，38：412-415）

关键词：哮喘鼻炎,变应性超敏反应变应原

过敏原组分检测在过敏性疾病预防与控制中的应用进展

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中华预防医学杂志 2021年第7期55卷 903-909页

作者：陈浩黄志锋罗文婷黄惠敏郑佩燕孙宝清华中科技大学同济医学院附属同济医院过敏反应科武汉430030 广州医科大学附属第一医院变态反应科 510120

我国过敏性疾病的发病率逐年升高,给个人和社会造成沉重的公共卫生负担。过敏原特异性IgE检测是过敏性疾病病因诊断的重要手段。目前,临床上主要针对过敏原粗提物的特异性IgE进行检测,而过敏原组分特异性IgE检测却开展甚少。临床医生尤... 详细信息

我国过敏性疾病的发病率逐年升高,给个人和社会造成沉重的公共卫生负担。过敏原特异性IgE检测是过敏性疾病病因诊断的重要手段。目前,临床上主要针对过敏原粗提物的特异性IgE进行检测,而过敏原组分特异性IgE检测却开展甚少。临床医生尤其是非过敏专科医生对过敏原组分特异性IgE检测的重要性认识不足。了解过敏原组分相关种类及临床意义,可提高临床医生对过敏性疾病的诊断水平。过敏原组分检测可以鉴别常见过敏原的主要组分、明确交叉致敏、预测严重过敏反应发生的风险、指导特异性免疫治疗以及为患者制定精准的饮食方案,从而为患者提供精确的、预防及控制建议。

关键词：过敏原组分诊断预防

Nanoparticles targeting at methylases with high correlation to N^(6)-methyladenosine-related lncRNA signatures as potential therapy of kidney clear cell carcinoma

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Chinese Chemical Letters 2022年第10期33卷 4610-4616页

作者： Ruixuan chen Ping Ouyang licong Su Xi Xu Penghu lian Yanqin li Qi Gao Yifan Zhang Sheng Nie Fan Luo Ruqi Xu Xiaodong Zhang Xiaoxi li Yue Cao peiyan Gao Juanjuan Kang Jun Wu Lu li National Clinical Research Center for Kidney Disease State Key Laboratory of Organ Failure ResearchGuangdong Provincial Clinical Research Center for Kidney DiseaseNanfang HospitalSouthern Medical UniversityGuangzhou 510000China Department of Health Management Nanfang HospitalSouthern Medical UniversityGuangzhou 510000China Department of Hematology Nanfang HospitalSouthern Medical UniversityGuangzhou 510000China Department of Urology Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical College(CAMS&PUMC)Beijing 100730China Department of Urology Nanfang HospitalSouthern Medical UniversityGuangzhou 510000China affiliated Foshan Maternity&Child Healthcare hospital Southern Medical UniversityFoshan 528000China School of Biomedical Engineering Sun Yat-sen UniversityShenzhen 518107China Department of Radiation Oncology Nanfang HospitalSouthern Medical UniversityGuangzhou 510000China

Clear cell renal cell carcinoma(ccRCC)is a heterogeneous malignancy with poor *** of the N^(6) position of adenosine(m^(6)A),the most common epigenetic modification in both messenger RNAs and noncoding RNAs,has been reported to regulate the initiation and progression of ***,whether and how m^(6)A-related long noncoding RNAs(m^(6)ArlncRNAs)signify the progression of ccRCC remain *** found m^(6)ArlncRNAs are effective signatures illustrating immune landscape and risk stratification in *** identified two differently expressed m^(6)ArlncRNAs(DEm^(6)ArlncRNAs),AC008870.2 and EMX2OS,as independent risk factors for overall survival of ccRCC patients,by applying stringent variable selection procedure to data from the Cancer Genome Atlas Kidney Renal Clear Cell Carcinoma *** risk score generated from the DEm^(6)ArlncRNA expression categorizes patients into either high or low-risk groups,between which,enrichment analysis indicated an enrichment in immune-related *** different DEm^(6)ArlncRNA transcription pattern,the two risk groups differ in immune cell population composition and expression levels of therapy targeting *** is satisfactory strategy to delivering therapeutic *** further clinical translation,we designed a novel nanoparticle delivery system packaged STM2457(STM@8P4 NPs),which selectively inhibits AC008870.2-correlated m^(6)A ***@8P4 NPs loaded drug successfully with uniform particle size,long-term stability and high release ***@8P4 NPs can easily enter ccRCC cells and showed a highly efficient ccRCC killing activity in *** results therefore indicate that m^(6)ArlncRNAs expression can depict tumor microenvironment,predict prognosis for ccRCC patient and give hint to therapeutic strategies in ccRCC.

关键词： Nanoparticle Methylation Long noncoding RNA Clear cell renal carcinoma Immunotherapy

Comparison between two commercial immunoassays:*** ALLERG-O-liQ versus Phadia ImmunoCAP~ System in detecting allergen-specific IgE and total IgE values

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Journal of Nanjing medical University 2008年第5期22卷 273-278页

作者： baoqing suna Mahler Michael Mei Jiang jing li Nanshan Zhong guangzhou Institute of Respiratory Diseases the First Affiliated Hospital of Guangzhou Medical College Guangzhou 510120 Guangdong province China Dr Fooke Laboratorien GmbH Neuss Germany

Objective： Beside patient＇ s history and skin prick testing（SPT） the detection of specific IgE（slgE） represents an important tool of allergy diagnostics. In recent years different technologies for the detection of slgE have been developed. The objective of this study is the comparison of the ALLERG-O-liQ with the ImmunoCAW System using seven inhalant and four food allergens. Methods： Sera from patients were collected and tested for slgE to inhalant（dl, d2, d5, i6, el, e5 and m3） and food allergens（fl, f2, f24, f24） by ALLERG-O-liQ（Dr. Fooke Laboratorien GmbH） and by ImmunoCAP System（Phadia）. Further, samples were also tested for total IgE in both systems. Results： Prevalence of positive test results varied between 0/20（f24） and 11/20（e5） for ALLERG-O-liQ and between 3/18（f23） and ll/20（dl/d5） for ImmunoCAP. The qualitative agreement between both methods was found between 75%（f24） and 100%（d2） depending on the allergen. Overall qualitative agreement for inhalant（n = 140）, food（n = 78） and all allergens（n = 218） tested was 92.1%（kappa = 0.84）, 83.3%（kappa = 0.58）, 89.0%（kappa = 0.77）, respectively. Sensitivity, specificity, positive predictive value （PPV）, negative predictive value（NPV） and diagnostic efficiency（DE） were found at 88.2%, 95.8%, 95.2%, 89.6%, 92.1%（inhalant allergens）, 62.5%, 92.6%, 78.9%, 84.7%, 83.3%（food allergens） and 81.5%, 94.4%, 91.5%, 87.5%, 89.0%（all allergens）. Conclusion： Good to excellent qualitative agreement between ALLERG-O-liQ and ImmunoCAP for the detection of specific and total IgE could be observed. The degree of agreement depended on the allergen and was higher in the group of inhalant allergens. The ALLERG-O-liQ System represents a reliable test for the detection of specific and total IgE.

关键词： specific IgE allergy diagnostics allergen

The therapeutics and prognosis of endometrial cancer

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The Chinese-German Journal of Clinical Oncology 2007年第3期6卷 290-292页

作者： Shousong huang peiyan Du Yunyu Wu lipai chen lixian chen Department of Gynecologic Oncology Clinical Tumor Research Center and Affiliated Tumor Hospital of GuangzhouMedical College Guangzhou 510095 China

Objective: To investigate the relative factors in the prognosis of endometrial cancer. Methods: From 1991 to 1999, 125 patients with endometrial cancer were treated in our hospital. Among them, 96 cases were received operation. Their clinical and pathological data were analyzed retrospectively. Results: The overall survival rate at 5-year were 77.6%, stage I to stage IV were 85.9%, 68.8%, 53.8% and 0%, respectively. The survival rates at 5-year in histological grade 1, grade 2 and grade 3 were 100%, 77.1% and 38.1% respectively. There’s no significant difference between grade 1 and grade 2 (P > 0.05). As to grade 1 and grade 3, grade 2 and grade 3, there were significant differences (P first line management of endometrial cancer. Clinical stage, histo- logical grade, depth of myometrial invasion and lymph node metastasis are relative factors of prognosis.

关键词： endometrial cancer therapy prognosis

广州地区热带无爪螨在呼吸道过敏性疾病的致敏模式

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实用医学杂志 2018年第10期34卷 1720-1724页

作者：刘晓清郑佩燕蔡传旭韦妮莉黄志锋邹湘玮孙宝清广州医科大学附属第一医院呼吸疾病国家重点实验室广州呼吸健康研究院变态反应科广州510120

目的探讨热带无爪螨在呼吸道过敏性疾病中的致敏模式,及其与过敏性鼻炎和(或)哮喘的联系。方法筛选广州地区尘螨致敏过敏性鼻炎和(或)哮喘患者330例,对其血清热带无爪螨、屋尘螨和粉尘螨的sIgE检测结果进行分析。结果热带无爪螨的致敏... 详细信息

目的探讨热带无爪螨在呼吸道过敏性疾病中的致敏模式,及其与过敏性鼻炎和(或)哮喘的联系。方法筛选广州地区尘螨致敏过敏性鼻炎和(或)哮喘患者330例,对其血清热带无爪螨、屋尘螨和粉尘螨的sIgE检测结果进行分析。结果热带无爪螨的致敏率均明显低于屋尘螨和粉尘螨(均P<0.001)。3种螨同时致敏者占80.0%,仅0.3%患者只对热带无爪螨致敏。3种螨的sIgE水平两两间均具有显著正相关性(P<0.001),屋尘螨与粉尘螨致敏高度相关(rs=0.906),热带无爪螨与屋尘螨、粉尘螨的致敏呈中度相关(rs=0.540和rs=0.512)。随着热带无爪螨sIgE水平的升高,合并屋尘螨或粉尘螨重度致敏(sIgE水平为5~6级)的阳性率均显著升高(P<0.001)。过敏性鼻炎伴哮喘患者的屋尘螨、粉尘螨sIgE显著高于单纯鼻炎、哮喘患者(均P<0.001),过敏性鼻炎伴哮喘和哮喘患者中,热带无爪螨sIgE水平显著高于鼻炎患者(P=0.006和P=0.020)。结论热带无爪螨致敏患者常合并屋尘螨和粉尘螨同时致敏,且哮喘患者热带无爪螨致敏程度更高。

关键词：热带无爪螨屋尘螨粉尘螨鼻炎哮喘