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CuS-PNIPAm nanoparticles with the ability to initiatively capture bacteria for photothermal treatment of infected skin

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Regenerative Biomaterials 2022年第1期9卷 347-359页

作者： Zizhen wang Zishuo Hou peiwen wang Fan Chen Xianglin Luo College of Polymer Science and Engineering Sichuan UniversityChengduPeople’s Republic of China State Key Laboratory of Polymer Materials Engineering Sichuan UniversityChengduPeople’s Republic of China

Copper sulfide nanoparticles(CuS NPs)have shown great potential in various application fields,especially in biomedical engineering *** NPs,with the ability to actively capture and kill bacteria and without the worry of biocompatibility,will greatly expand their ***,a four-arm star thermosensitive polyisopropylacrylamide(4sPNIPAm)was used to modify CuS NPs(CuS-PNIPAm NPs).The obtained NPs displayed the controlled release of copper ions and higher photothermal conversion ability in comparison with contrast materials CuS-PEG NPs and CuS *** of CuS-PNIPAm NPs at above 34℃resulted in capturing bacteria by forming the aggregates of *** Staphylococcus aureus and Escherichia coli co-cultured with CuS-PNIPAm NPs were completely killed upon near-infrared irradiation in ***,CuS-PNIPAm NPs were verified to be a photothermal agent without toxic *** in vivo experiment,the NPs effectively killed the bacteria in the wound and accelerated the process of wound ***,photothermal treatment by CuS-PNIPAm NPs demonstrates the ability to actively capture and kill bacteria,and has a potential in the treatment of infected skin and the regeneration of skin *** therapy will exert a far-reaching impact on the regeneration of stubborn chronic wounds.

关键词： capturing bacteria wound healing acceleration photothermal ablation ion-controlled release

Preparation of Large Area Double Sided YBCO Thin Films by DC Magneton Sputtering

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Journal of Rare Earths 2004年第Z1期22卷 249-251页

作者： Li Tao Gu Hongwei wang peiwen wang Xiaoping Xie Bowei Superconducting Materials Research Center General Research Institute for Non-Ferrous Metals Beijing 100088 China

Tc, Jc and Rs properties of large area double sided YBCO thin films deposited on LaAlO3 substrates by direct current sputtering were *** thickness of the obtained thin films is over 300 nm, Tc > 90 K; Rs can be as low as less than 1.0 mΩ(77 K, 10 GHz).Homogenousity of the properties around the plane was *** the measnous *** of substrate temperature and Ar and O2 pressures on properties of the double-sided thin films were discussed.

关键词： large area double sided superconductor thin film YBCO magneton sputtering

The transcription factor SlHY5 regulates the ripening of tomato fruit at both the transcriptional and translational levels

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Horticulture Research 2021年第1期8卷 939-953页

作者： Weihao wang peiwen wang Xiaojing Li Yuying wang Shiping Tian Guozheng Qin Key Laboratory of Plant Resources Institute of Botanythe Innovative Academy of Seed DesignChinese Academy of SciencesNo.20 NanxincunXiangshanHaidian District100093 BeijingChina University of Chinese Academy of Sciences 100049 BeijingChina

Light plays a critical role in plant growth and development,but the mechanisms through which light regulates fruit ripening and nutritional quality in horticultural crops remain largely ***,we found that ELONGATED HYPOCOTYL 5(HY5),a master regulator in the light signaling pathway,is required for normal fruit ripening in tomato(Solanum lycopersicum).Loss of function of tomato HY5(SlHY5)impairs pigment accumulation and ethylene *** profiling identified 2948 differentially expressed genes,which included 1424 downregulated and 1524 upregulated genes,in the Slhy5 *** addition,genes involved in carotenoid and anthocyanin biosynthesis and ethylene signaling were revealed as direct targets of SlHY5 by chromatin ***,the expression of a large proportion of genes encoding ribosomal proteins was downregulated in the Slhy5 mutants,and this downregulation pattern was accompanied by a decrease in the abundance of ribosomal *** analysis demonstrated that SlHY5 affected the translation efficiency of numerous ripening-related *** data indicate that SlHY5 regulates fruit ripening both at the transcriptional level by targeting specific molecular pathways and at the translational level by affecting the protein translation *** findings unravel the regulatory mechanisms of SlHY5 in controlling fruit ripening and nutritional quality and uncover the multifaceted regulation of gene expression by transcription factors.

关键词： HY5 transcriptional translational

Genome-wide binding analysis of the tomato transcription factor SlDof1 reveals its regulatory impacts on fruit ripening

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Molecular Horticulture 2021年第1期1卷 90-107页

作者： Yuying wang peiwen wang Weihao wang Lingxi Kong Shiping Tian Guozheng Qin Key Laboratory of Plant Resources Institute of BotanyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100093China University of Chinese Academy of Sciences Beijing 100049China

The DNA binding with one finger(Dof)proteins are plant-specific transcription factors involved in a variety of biological ***,little is known about their functions in fruit ripening,a flowering-plant-specific process that is required for seed maturation and ***,we found that the tomato Dof transcription factor SlDof1,is necessary for normal fruit *** of SlDof1 expression by RNA interference delayed ripening-related processes,including lycopene synthesis and ethylene *** profiling indicated that SlDof1 influences the expression of hundreds of genes,and a chromatin immunoprecipitation sequencing revealed a large number of SlDof1 binding sites.A total of 312 genes were identified as direct targets of SlDof1,among which 162 were negatively regulated by SlDof1 and 150 were positively *** SlDof1 target genes were involved in a variety of metabolic pathways,and follow-up analyses verified that SlDof1 directly regulates some well-known ripening-related genes including ACS2 and PG2A as well as transcriptional repressor genes such as *** findings provide insights into the transcriptional regulatory networks underlying fruit ripening and highlight a gene potentially useful for genetic engineering to control ripening.

关键词： Tomato Fruit ripening Transcriptional regulation DNA binding with one finger(Dof) chromatin immunoprecipitation followed by sequencing(ChIP-seq) RNA sequencing(RNA-seq)

DIA-based proteome profiling with PRM verification reveals the involvement of ER-associated protein processing in pollen abortion in Ogura CMS cabbage

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Horticultural Plant Journal 2024年第3期10卷 755-770页

作者： peiwen wang Lin Zhu Ziheng Li Mozhen Cheng Xiuling Chen Aoxue wang Chao wang Xiaoxuan Zhang Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region) Ministry of Agriculture and Rural AffairsNortheast Agricultural UniversityHarbinHeilongjiang 150030China College of Horticulture and Landscape Architecture Northeast Agricultural UniversityHarbinHeilongjiang 150030China

Ogura cytoplasmic male sterility(Ogura CMS)is extensively applied in hybrid seed production in cruciferous ***,the posttranscriptional molecular basis of Ogura CMS in cruciferous crops remains ***,a data-independent acquisition-based proteomic approach coupled with a parallel reaction monitoring-based targeted proteomic assay was used to analyze the proteome dynamics of Ogura CMS cabbage line RM and its maintainer line RF during floral bud development to obtain insights into the mechanism underlying Ogura CMS in cruciferous crops.A total of 9162 proteins corresponding to 61464 peptides were identified in RM and RF floral *** proteomic fluctuation of RM was weaker than that of *** in protein expression between RM and RF gradually enlarged with floral bud *** continually up-regulated and eight continually down-regulated proteins were found in RM relative to RF throughout floral bud *** expressed proteins between RM and RF during floral bud development were implicated in the endoplasmic reticulum(ER)-associated protein processing pathway,in which most of them exhibited down-regulated expression in *** data suggest that ER-associated protein processing may be involved in pollen abortion in Ogura CMS cabbage by inhibiting the expression of critical *** findings not only deepen the understanding of the molecular mechanisms of Ogura CMS in cruciferous crops but also provide better guidance for applying Ogura CMS in the hybrid breeding of cruciferous crops.

关键词： Ogura cytoplasmic male sterility(Ogura CMS) Cabbage Data-independent acquisition(DIA) Parallel reaction monitoring(PRM) Pollen development

The Normalized Laplacian Spectrum of Pentagonal Graphs and Its Applications

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Journal of Mathematical Research with Applications 2019年第4期39卷 343-352页

作者： Xiaojing XU peiwen wang Zhiping wang Faculty of Science Dalian Maritime University College of Shipping Economics and Management Dalian Maritime University

The eigenvalues of the normalized Laplacian of a graph provide information on its structural properties and also on some relevant dynamical aspects, in particular those related to random walks. In this paper, we give the spectra of the normalized Laplacian of iterated pentagonal of a simple connected graph. As an application, we also find the significant formulae for their multiplicative degree-Kirchhoffindex, Kemeny’s constant and number of spanning trees.

关键词： normalized Laplacian spectrum multiplicative degree-Kirchhoff index Kemeny’s constant the number of spanning trees

Optimization of Rolling Process for Bi(2223)/Ag Superconducting Tapes by a Statistical Method

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Rare Metals 2001年第1期20卷 66-68页

作者： Xuefeng Li, peiwen Hua, Wenquan wang, Qijian Jin, Yiru Zhou, Guansen Yuan (General Research Institute for Nonferrous Metals, Beijing 100088, China) 北京有色冶金研究总院北京 100088

Ag-sheathed (Bi,Pb)(2)SoCa(2)Cu(3)O(x) tapes were prepared by the powder-in-tube method. The influences of rolling parameters on superconducting characteristics of Bi(2223)/Ag tapes were analyzed qualitatively with a statistical method. The results demonstrate that roll diameter and reduction per pass significantly influence the properties of Bi(2223)/Ag superconducting tapes while roll speed does less and working friction the least. An optimized rolling process was therefore achieved according to the above results.

关键词： Bi(2223)/Ag multifilamentary tapes rolling process statistical method optimization

Prediction of the intramuscular fat content of pork cuts by improved U2-Net model and clustering algorithm

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Food Bioscience 2023年第3期53卷 3136-3147页

作者： Hu Liu Wei Zhan Zhiqiang Du Mengyuan Xiong Tao Han peiwen wang Weihao Li Yong Sun School of Computer Science Yangtze UniversityJingzhou434023China School of Animal Science Yangtze UniversityJingzhou434025China

The intramuscular fat content is an essential indicator of pork quality,directly affecting sensory quality and consumers’willingness to *** testing methods are subjective and destructive,their assessment is scored by trained assessors according to a marbling scale,but the human sensory evaluation has great subjectivity and ***,there are more methods to predict the intramuscular fat content of livestock meat using computer vision ***,the complex background of the image makes it difficult to segment the target and *** study proposes a method based on semantic segmentation networks and machine learning algorithms to detect the intramuscular fat content of multi-part pork *** images of five different pork cuts(belly,loin,collar,ham,and hock)are used as experimental data in the study,the results show that the method proposed in this paper can objectively detect the intramuscular fat content of pork and the average accuracy of prediction can reach 93.28%.This new information method can be used to assess the quality of pork in markets and food processing plants,enabling processors and consumers to distinguish pork cuts with different fat *** improves the quality of pork to meet the needs of the food processing industry.

关键词： Multi-part pork cuts Predicting pork intramuscular fat Semantic segmentation Machine learning

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河南墨学研究

前沿 2010年第8期 51-54页

作者：王培文平顶山学院思想政治理论教学部河南平顶山467002

自20世纪90年代,特别是"河南省墨子学会"成立以来,河南省学者认真进行学术研究,积极开展学术交流,出版和发表了一大批高质量的学术论著和论文,壮大了队伍,扩大了影响,在全国墨学界有一席之地,初步实现了"墨学研究"的中原崛起。

关键词：墨子墨学研究河南崛起