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Simultaneous nitrification and denitrification conducted by Halomonas venusta MA-ZP17-13 under low temperature conditions

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Acta Oceanologica Sinica 2021年第9期40卷 94-104页

作者： Guizhen Li Qiliang Lai Guangshan Wei Peisheng Yan Zongze Shao School of Environment Harbin Institute of TechnologyHarbin 150090China Key Laboratory of Marine Genetic Resources Third Institute of OceanographyMinistry of Natural ResourcesXiamen 361005China State Key Laboratory Breeding Base of Marine Genetic Resources Xiamen 361005China Fujian Key Laboratory of Marine Genetic Resources Xiamen 361005China Sourthem Marine Science and Engineering Guangdong Laboratory(Zhuhai) Zhuhai 519080China

Nitrification is a key step in the global nitrogen cycle.Compared with autotrophic nitrification,heterotrophic nitrification remains poorly understood.In this study,Halomonas venusta MA-ZP17-13,isolated from seawater in shrimp aquaculture (Penaeus vannamei),could simultaneously undertake nitrification and denitrification.With the initial ammonium concentration at 100 mg/L,the maximum ammonium-nitrogen removal rate reached98.7%under the optimal conditions including C/N concentration ratio at 5.95,p H at 8.93,and Na Cl at 2.33%.The corresponding average removal rate was 1.37 mg/(L·h)(according to nitrogen) in 3 d at 11.2℃.By whole genome sequencing and analysis,nitrification-and denitrification-related genes were identified,including ammonia monooxygenase,nitrate reductase,nitrite reductase,nitric oxide dioxygenase and nitric oxide synthase;while no gene encoding hydroxylamine oxidase was identified,it implied the existence of a novel nitrification pathway from hydroxylamine to nitrate.These results indicate heterotrophic bacterium H.venusta MA-ZP17-13 can undertake simultaneous nitrification and denitrification at low temperature and has potential for NH_(4)^(+)-N/NH_(3)-N removal in marine aquaculture systems.

关键词： Halomonas heterotrophic nitrification denitrification ammonia oxidization ammonia removal

Effect of Cultural Conditions on Chitinase Production from Biocontrol Bacterium Against Aflatoxin

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Journal of Harbin Institute of Technology(New Series) 2015年第3期22卷 82-86页

作者： Kai Wang Peisheng Yan Lixin Cao School of Municipal and Environmental Engineering Harbin Institute of Technology School of Marine Science and Technology Harbin Institute of Technology

Chitinase is one of the most important mycolytic enzymes with industrial significance. Statistical methods are employed to optimize cultural conditions with the increased production of chitinase for the selected Serratia marcescens JPP1,which are obtained from peanut hulls in Jiangsu Province, China and exhibit antagonistic activity against aflatoxins. Using single-factor experiments the effects of cultural conditions( broth content,inoculum size and rotation speed) on chitinase production from S. marcescens JPP1 are evaluated. Central composite design of Response Surface Methodology is used to optimize the levels of factors for the best yield of enzymes production. The optimized cultural conditions for obtaining the highest level of chitinase production are23. 2 mL broth content,116 r / min rotation speed and 4. 3% inoculum size. A quadratic regression model of chitinase production is built( R2= 0. 970 9) and the verification experiments confirm its validity. The maximum chitinase production obtained after the optimization is 29. 58 U / mL for a 1. 4-fold increase.

关键词： chitinase aflatoxin optimization Central Composite Design

Potential of Novel Halotolerant and Psychrotrophic Strain of Planococcus sp. for Water Treatment

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Journal of Harbin Institute of Technology(New Series) 2018年第5期25卷 24-32页

作者： Kai Wang Siqi Li Xiquan Cheng Yingjie Zhang Peisheng Yan Jun Ma School of Marine Science and Technology Harbin Institute of Technology School of Municipal and Environmental Engineering Harbin Institute of Technology

To screen multifunctional bacteria for water treatment,a chemical oxygen demand( COD)degradation and flocculation strain S2 A15 was obtained from the offshore sewage outfall at Weihai International Beach. Based on the phylogenetic characteristics,a novel strain was identified as a Planococcus species. Strain S2 A15 was determined to have the ability of flocculation and COD degradation. A series of experiments showed that the strain S2 A15 could be used for the treatment of four types of wastewater,including domestic wastewater( 400 mg/L and 800 mg/L) and high salt domestic wastewater( 400 mg/L and 800 mg/L). Among them,the best effect was exerted by the strain that reduced by 76.9% in domestic wastewater with 400 mg/L COD. The flocculation ratio reached 60.19%. The optimal treatment conditions are also discussed. We confirmed that the strain S2 A15 had salt tolerance and low temperature resistance. The best growth of S2 A15 at salt concentration of 6% and further confirmed that the strain could degrade COD at a low temperature.

关键词： COD flocculation wastewater treatment Plauococcus sp. salt-tolerant bacteria

金沙江流域云南段地震灾害人口风险性分布多维度识别办法

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灾害学 2021年第4期36卷 96-100页

作者：严佩升昭通学院地理科学与旅游学院云南昭通657000

研究金沙江流域云南段地震灾害人口风险性分布多维度识别办法,提升该地区地震发生时救援能力以及应灾、防灾、救灾能力。通过定量确定人口风险性分布多维度识别的风险性影响因子,其中人口易损系数着重探讨人口分布、人口老幼比例、人口... 详细信息

研究金沙江流域云南段地震灾害人口风险性分布多维度识别办法,提升该地区地震发生时救援能力以及应灾、防灾、救灾能力。通过定量确定人口风险性分布多维度识别的风险性影响因子,其中人口易损系数着重探讨人口分布、人口老幼比例、人口迁移,获得地震灾区人口分布规律,确保该风险因子足够精准。赋权各风险因子构建风险性识别模型,使用空间叠加分析功能,获得地震人口风险分布结果。运用ArcMap软件,计算地震灾害中包含的人口因素,构建研究区域发生地震时的人口风险性分布图,在图中将人口高风险分布区划分出来,以便该地区有关部门使用该办法应对可能发生的地震灾害。

关键词：金沙江云南段地震灾害人口风险性分布多维度识别

抑制黄曲霉毒素的深海环状芽胞杆菌发酵条件优化

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应用海洋学学报 2022年第4期41卷 693-700页

作者：徐静静闫培生哈尔滨工业大学环境学院黑龙江哈尔滨150090 哈尔滨工业大学(威海)海洋科学与技术学院山东威海264209

环状芽胞杆菌(Bacillus circulans)菌株FA13在M2培养基中的发酵无细胞上清液对寄生曲霉(Aspergillus parasiticus)突变菌株NFRI-95菌丝生长和黄曲霉毒素的产生表现出明显的抑制作用。为了最大限度地提高活性物质的产量,本研究对从南大... 详细信息

环状芽胞杆菌(Bacillus circulans)菌株FA13在M2培养基中的发酵无细胞上清液对寄生曲霉(Aspergillus parasiticus)突变菌株NFRI-95菌丝生长和黄曲霉毒素的产生表现出明显的抑制作用。为了最大限度地提高活性物质的产量,本研究对从南大西洋深海沉积物中分离到的环状芽胞杆菌菌株FA13的发酵条件进行了均匀设计发酵优化。优化后,菌株FA13的发酵液10倍稀释液对寄生曲霉突变菌株NFRI-95的菌丝生长抑制率从10.98%提高到70.57%,黄曲霉毒素产生抑制率从41.43%提高到100.00%;对应的发酵条件为发酵温度38.70℃,接种量6.00%,海水体积占比0.00%,培养基pH 8.71,培养时间6 d。

关键词：海洋生物学环状芽胞杆菌抑制产毒寄生曲霉突变菌株黄曲霉毒素发酵工艺优化

Inhibition of Aspergillus parasiticus and Cancer Cells by Marine Actinomycete Strains

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Journal of Ocean University of China 2014年第6期13卷 985-994页

作者： LI Ping yan peisheng School of Municipal and Environmental Engineering Harbin Institute of Technology School of Marine Science and Technology Harbin Institute of Technology at Weihai

Ten actinomycete strains isolated from the Yellow Sea off China's coasts were identified as belonging to two genera by 16 S r DNA phylogenetic analysis: Streptomyces and Nocardiopsis. Six Streptomyces strains(MA10, 2SHXF01-3, MA35, MA05-2, MA05-2-1 and MA08-1) and one Nocardiopsis strain(MA03) were predicted to have the potential to produce aromatic polyketides based on the analysis of the KSα(ketoacyl-synthase) gene in the type II PKS(polyketides synthase) gene cluster. Four strains(MA03, MA01, MA10 and MA05-2) exhibited significant inhibitory effects on mycelia growth(inhibition rate >50%) and subsequent aflatoxin production(inhibition rate >75%) of the mutant aflatoxigenic Aspergillus parasiticus NFRI-95. The ethyl acetate extracts of the broth of these four strains displayed significant inhibitory effects on mycelia growth, and the IC50 values were calculated(MA03: 0.275 mg m L-1, MA01: 0.106 mg m L-1, MA10: 1.345 mg m L-1 and MA05-2: 1.362 mg m L-1). Five strains(2SHXF01-3, MA03, MA05-2, MA01 and MA08-1) were selected based on their high cytotoxic activities. The ethyl acetate extract of the Nocardiopsis strain MA03 was particularly noted for its high antitumor activity against human carcinomas of the cervix(He La), lung(A549), kidney(Caki-1) and liver(Hep G2)(IC50: 2.890, 1.981, 3.032 and 2.603 μg m L-1, respectively). The extract also remarkably inhibited colony formation of He La cells at an extremely low concentration(0.5 μg m L-1). This study highlights that marine-derived actinomycetes are a huge resource of compounds for the biological control of aflatoxin contamination and the development of novel drugs for human carcinomas.

关键词：海洋放线菌菌株分离寄生曲霉癌细胞聚酮化合物 HeLa细胞诺卡氏菌属抗肿瘤活性

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公共资产配置与预算管理的演化博弈分析

系统工程理论与实践 2020年第11期40卷 2872-2884页

作者：严培胜王先甲张青湖北经济学院信息管理与统计学院武汉430205 武汉大学经济与管理学院武汉430072 武汉大学系统工程研究所武汉430072 湖北经济学院财政与公共管理学院武汉430205

在公共资产配置与预算管理过程中,由于财政部门和预算单位之间存在信息不对称,导致公共资产配置效率和使用效益低下.本文运用演化博弈的理论与方法分析了公共资产配置与预算管理的博弈演化过程,研究了预算管理过程中的声誉激励效应和监... 详细信息

在公共资产配置与预算管理过程中,由于财政部门和预算单位之间存在信息不对称,导致公共资产配置效率和使用效益低下.本文运用演化博弈的理论与方法分析了公共资产配置与预算管理的博弈演化过程,研究了预算管理过程中的声誉激励效应和监督惩罚效应,给出了不同类型预算单位与财政部门之间策略选择的互动机制,重点对静态惩罚机制和动态惩罚机制下博弈均衡进行了比较.研究表明:声誉对高成本的预算单位具有激励作用,监督对低成本的预算单位具有惩罚作用,动态惩罚机制比静态惩罚机制的监督效果更好,监督概率和监督惩罚量越大监督效果越好.

关键词：公共资产部门预算演化博弈

Identification of alginate lyase-excreting microorganisms associated with the brown algae and comparison of their alginate-degrading activities

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Identification of alginate lyase-excreting microorganisms as...

海带渣废弃物资源化利用以及多功能菌肥固体发酵条件的优化

中国生物工程学会第十届学术年会暨2016年全国生物技术...

作者： Mingpeng Wang Lei Chen Zhaojie Zhang Feng Li Xuejiang Wang Song Qin Peisheng Yan School of Municipal and Environmental Engineering Harbin Institute of Technology yantai Institute of Costal Zone Research Chinese Academy of Sciences Department of Zoology and Physiology University of Wyoming

As one of algal tool enzymes, alginate lyase is a promising biocatalyst because of its application in saccharification of alginate for the production of oligosaccharides with bioactive function and renewable biofuels.

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环境工程学报 2014年第11期8卷 4984-4990页

作者：肖伟闫培生哈尔滨工业大学海洋科学与技术学院威海264209

海带渣是海带加工过程中主要固体废弃物。本实验利用海带渣对生防菌株JGA2(-5)27-2进行固体发酵。实验对不同发酵条件下发酵产物对植物病原真菌寄生曲霉的抑制活性与对发酵产植物激素6-糠氨基嘌呤的能力进行测定。通过单因素实验和响应... 详细信息

海带渣是海带加工过程中主要固体废弃物。本实验利用海带渣对生防菌株JGA2(-5)27-2进行固体发酵。实验对不同发酵条件下发酵产物对植物病原真菌寄生曲霉的抑制活性与对发酵产植物激素6-糠氨基嘌呤的能力进行测定。通过单因素实验和响应面实验优化了发酵条件:蔗糖为最优的碳源;硝酸盐为最优的氮源;蔗糖浓度、KNO3浓度、最佳的培养时间、含水量、培养温度、接种量和pH值分别为2.091%、0.507%、8.24 d、60%、35℃、4%和自然pH值。在发酵过程中以添加KNO3的形式向发酵菌肥中加入K+离子。实验证明,利用生防菌株JGA2(-5)27-2进行海带渣的固体发酵生产多功能微生物肥料具有可行性,并为海带渣的资源化在农业领域的利用提供一定参考。

关键词：海带渣固体发酵微生物肥料资源化利用