Effect of Cultural Conditions on Chitinase Production from Biocontrol Bacterium Against Aflatoxin

作     者：Kai Wang Peisheng Yan Lixin Cao 

作者机构：School of Municipal and Environmental EngineeringHarbin Institute of Technology School of Marine Science and TechnologyHarbin Institute of Technology 

出 版 物：《Journal of Harbin Institute of Technology(New Series)》 (哈尔滨工业大学学报（英文版）)

年 卷 期：2015年第22卷第3期

页      面：82-86页

核心收录：

学科分类：0810[工学-信息与通信工程] 07[理学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0713[理学-生态学] 0812[工学-计算机科学与技术（可授工学、理学学位）] 

基　　金：Sponsored by the National Natural Science Foundation of China(Grant No.30870003,21273056) the Foundation for Outstanding Yong Scientist in Shandong Province(Grant No.BS2014NY012) 

主　　题：chitinase aflatoxin optimization Central Composite Design 

摘      要：Chitinase is one of the most important mycolytic enzymes with industrial significance. Statistical methods are employed to optimize cultural conditions with the increased production of chitinase for the selected Serratia marcescens JPP1,which are obtained from peanut hulls in Jiangsu Province, China and exhibit antagonistic activity against aflatoxins. Using single-factor experiments the effects of cultural conditions( broth content,inoculum size and rotation speed) on chitinase production from S. marcescens JPP1 are evaluated. Central composite design of Response Surface Methodology is used to optimize the levels of factors for the best yield of enzymes production. The optimized cultural conditions for obtaining the highest level of chitinase production are23. 2 mL broth content,116 r / min rotation speed and 4. 3% inoculum size. A quadratic regression model of chitinase production is built( R2= 0. 970 9) and the verification experiments confirm its validity. The maximum chitinase production obtained after the optimization is 29. 58 U / mL for a 1. 4-fold increase.