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Laccase/caffeic acid-catalyzed crosslinking coupled with galactomannan alters the conformational structure of ovalbumin and alleviates Th2-mediated allergic asthma

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Food Science and Human Wellness 2024年第4期13卷 1962-1973页

作者： Ishfaq Ahmed Suidong Ouyang Shengquan Wu Haochang Song Miaoyuan Zhang Renxing luo peishan lu Jiaqi Deng Tingting Zheng Yanyan Wang Xinguang Liu Gonghua Huang Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics The First Dongguan Affiliated HospitalGuangdong Medical UniversityDongguan 523808China Haide College Ocean University of ChinaLaoshan CampusQingdao 266100China School of Medical Technology Guangdong Medical UniversityDongguan 523808China

Ovalbumin(OVA)is the major allergenic protein that can induce T helper 2(Th2)-allergic reactions,for which current treatment options are inadequate.In this study,we developed a polymerized hypoallergenic OVA product via laccase/caffeic acid(Lac/CA)-catalyzed crosslinking in conjunction with galactomannan(Man).The formation of high molecular weight crosslinked polymers and the Ig G-binding were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE)and Western blotting.The study indicated that Lac/CA-catalyzed crosslinking plus Man conjugation substantially altered secondary and tertiary structures of OVA along with the variation in surface hydrophobicity.Gastrointestinal digestion stability assay indicated that crosslinked OVA exhibited less resistance in simulated gastric fluid(SGF)and simulated intestinal fluid(SIF).Mouse model study indicated that Lac-Man/OVA ameliorated eosinophilic airway inflammatory response and efficiently downregulated the expression of Th2-related cytokines(interleukin(IL)-4,IL-5,and IL-13),and upregulated IFN-γand IL-10 expression.Stimulation of bone marrow-derived dendritic cells with Lac-Man/OVA suppressed the expression of phenotypic maturation markers(CD80 and CD86)and MHC class II molecules,and suppressed the expression levels of proinflammatory cytokines.The knowledge obtained in the present study offers an effective way to acquire a hypoallergenic OVA product that can have a therapeutic effect in alleviating OVA-induced allergic asthma.

关键词： Ovalbumin Laccase Galactomannan Conformational structure Asthma

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The kinase p38αfunctions in dendritic cells to regulate Th2-cell differentiation and allergic inflammation

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Cellular & Molecular Immunology 2022年第7期19卷 805-819页

作者： Miaomiao Han Jingyu Ma Suidong Ouyang Yanyan Wang Tingting Zheng peishan lu Zihan Zheng Weiheng Zhao Hongjin Li Yun Wu Baohua Zhang Ran Hu Kinya Otsu Xinguang Liu Ying Wan Huabin Li Gonghua Huang ENT Institute and Department of Otorhinolaryngology Eye&ENT HospitalFudan University200031 ShanghaiChina Shanghai Institute of Immunology Shanghai Jiao Tong University School of Medicine200025 ShanghaiChina Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics Guangdong Medical University523808 DongguanChina Biomedical Analysis Center Army Medical University400038 ChongqingChina Department of Cardiovascular Medicine Graduate School of MedicineOsaka UniversityOsaka 565-0871Japan School of Cardiovascular Medicine and Sciences King’s College LondonLondon SE59NUUK Present address:Tongji Hospital Tongji Medical CollegeHuazhong University of Science and Technology430030 WuhanChina Department of Dermatology Yueyang Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese Medicine200437 ShanghaiChina Department of Clinical Laboratory Medicine Shanghai Tenth People’s Hospital of Tongji University200072 ShanghaiChina Present address:Basic Department of Cancer Center Shanghai Tenth People’s Hospital of Tongji University200072 ShanghaiChina

Dendritic cells(DCs)play a critical role in controlling T helper 2(Th2)cell-dependent diseases,but the signaling mechanism that triggers this function is not fully understood.We showed that p38αactivity in DCs was decreased upon HDM stimulation and dynamically regulated by both extrinsic signals and Th2-instructive cytokines.p38α-specific deletion in cDC1s but not in cDC2s or macrophages promoted Th2 responses under HDM stimulation.Further study showed that p38αin cDC1s regulated Th2-cell differentiation by modulating the MK2−c-FOS−IL-12 axis.Importantly,crosstalk between p38α-dependent DCs and Th2 cells occurred during the sensitization phase,not the effector phase,and was conserved between mice and humans.Our results identify p38αsignaling as a central pathway in DCs that integrates allergic and parasitic instructive signals with Th2-instructive cytokines from the microenvironment to regulate Th2-cell differentiation and function,and this finding may offer a novel strategy for the treatment of allergic diseases and parasitic infection.

关键词： Allergy p38α dendritic cell Th2

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Laccase/caffeic acid-catalyzed crosslinking coupled with galactomannan alters the conformational structure of OVA and alleviates Th2-mediated allergic asthma

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Food Science and Human Wellness 2023年

作者： Ishfaq Ahmed Suidong Ouyang Shengquan Wu Haochang Song Miaoyuan Zhang Renxing luo peishan lu Jiaqi Deng Tingting Zheng Yanyan Wang Xinguang Liu Gonghua Huang School of Medical Technology Guangdong Medical University Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics The First Dongguan Affiliated Hospital Guangdong Medical University Haide College Ocean University of China

Ovalbumin (OVA) is the major allergenic protein that can induce T helper 2 (Th2)-allergic reactions for which current treatment options are inadequate. In this study， we developed a polymerized hypoallergenic OVA product via laccase/caffeic acid (Lac/CA)-catalyzed crosslinking in conjunction with galactomannan (Man). The formation of high molecular weight crosslinked polymers and the IgG-binding were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting. The study indicated that Lac/CA-catalyzed crosslinking plus Man conjugation substantially altered secondary and tertiary structures of OVA along with the variation in surface hydrophobicity. Gastrointestinal digestion stability assay indicated that crosslinked OVA exhibited less resistance in simulated gastric fluid (SGF) and simulated intestinal fluid (SIF). Mouse model study indicated that Lac-Man/OVA ameliorated eosinophilic airway inflammatory response and efficiently downregulated the expression of Th2-related cytokines (IL-4， IL-5， and IL-13)， and upregulated IFN-γ and IL-10 expression. Stimulation of bone marrow-derived dendritic cells with Lac-Man/OVA suppressed the expression of phenotypic maturation markers (CD80， and CD86) and MHC class II molecules， and suppressed the expression levels of proinflammatory cytokines. The knowledge obtained in the present study offers an effective way to acquire a hypoallergenic OVA product that can have a therapeutic effect in alleviating OVA-induced allergic asthma.

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A teleconnection pattern of upper-tropospheric circulation anomalies over the Eurasian continent associated with the interannual variability of atmospheric convection over the tropical western North Pacific in July

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Atmospheric and Oceanic Science Letters 2024年第3期17卷 20-24页

作者： peishan Chen Riyu lu State Key Laboratory of Numerical Modeling for Atmospheric Sciences and Geophysical Fluid Dynamics Institute of Atmospheric PhysicsChinese Academy of Sciences BeijingChina College of Earth and Planetary Sciences University of Chinese Academy of SciencesBeijingChina

热带西北太平洋大气对流的年际变化对东亚夏季气候有着显著影响,而且受到前冬ENSO的影响.然而,还有相当多的西北太平洋对流年际变化不能由前冬ENSO解释.本文表明,7月西北太平洋对流受到欧亚大陆对流层上层遥相关型的显著影响,且这一影... 详细信息

热带西北太平洋大气对流的年际变化对东亚夏季气候有着显著影响,而且受到前冬ENSO的影响.然而,还有相当多的西北太平洋对流年际变化不能由前冬ENSO解释.本文表明,7月西北太平洋对流受到欧亚大陆对流层上层遥相关型的显著影响,且这一影响与前冬ENSO的影响程度相当.该遥相关型表现为位于欧洲的气旋式环流异常,中亚的反气旋式环流异常和东亚带状延伸的气旋式环流异常,并表现为7月对流层上层环流的第一主模态.基于本文的结果并结合前人的研究,作者认为这一遥相关型能通过亚洲急流出口区附近的罗斯贝波破碎引起西北太平洋对流异常.

关键词：遥相关大气对流西北太平洋年际变化

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江苏省2011-2019年风疹病毒流行株基因特征

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中国疫苗和免疫 2022年第4期28卷 395-401页

作者：邓秀英胡莹陆培善郭宏雄江苏省疾病预防控制中心江苏南京210009

目的分析江苏省2011-2019年风疹病毒(Rubella virus,RV)流行株基因型和基因亚型。方法对2011-2019年江苏省风疹报告病例采集咽拭子标本,进行RV分离、E1基因核苷酸序列扩增和测定,与世界卫生组织各基因型RV参考株构建亲缘关系树,鉴定RV... 详细信息

目的分析江苏省2011-2019年风疹病毒(Rubella virus,RV)流行株基因型和基因亚型。方法对2011-2019年江苏省风疹报告病例采集咽拭子标本,进行RV分离、E1基因核苷酸序列扩增和测定,与世界卫生组织各基因型RV参考株构建亲缘关系树,鉴定RV基因型和基因亚型,分析氨基酸变异。结果从江苏省2011-2019年风疹病例中共分离到19株RV,其中1E基因型13株,包括1E-L1基因亚型7株、1E-L2基因亚型6株;2B基因型6株,均为2B-L1基因亚型。与BRD-II疫苗株相比,1E基因型主要出现A333T(2019年)、L338F(2011-2013年)和L338S(2019年)变异;2B基因型主要出现A333V(2011-2015年)变异。结论江苏省2011-2019年RV流行株出现2个基因亚型传播链更替,即2015年2B-L1取代1E-L1和2019年1E-L2取代2B-L1;需进一步加强RV分子病毒学监测。

关键词：风疹病毒基因型基因亚型基因特征

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2018-2020年江苏省健康人群麻疹抗体水平监测分析

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疾病监测 2022年第10期37卷 1299-1303页

作者：胡莹陆培善邓秀英郭宏雄江苏省疾病预防控制中心江苏南京210009

目的了解和掌握2018—2020年江苏省不同年龄组健康人群的麻疹免疫情况,对其麻疹抗体水平进行监测,并结合近年麻疹发病情况,为麻疹消除提供依据。方法选择2018—2020年江苏省7个市/县的健康人群,按年龄分为0~7月龄、8月龄至4岁、5~14岁、... 详细信息

目的了解和掌握2018—2020年江苏省不同年龄组健康人群的麻疹免疫情况,对其麻疹抗体水平进行监测,并结合近年麻疹发病情况,为麻疹消除提供依据。方法选择2018—2020年江苏省7个市/县的健康人群,按年龄分为0~7月龄、8月龄至4岁、5~14岁、15~24岁、25~39岁、40~60岁共6个组。使用酶联免疫吸附试验(ELISA)检测麻疹IgG抗体并进行定量换算,计算其抗体阳性率、高浓度抗体率、几何平均浓度(GMC)。结果共检测标本3132份,抽样人群的抗体阳性率为79.25%,GMC为491.02 mIU/mL,高浓度抗体样品1267份,占40.45%。具有1~4剂次含麻疹成分疫苗接种史的人群中,IgG抗体阳性率为90.34%,GMC为741.31 mIU/mL。各年龄组之间麻疹IgG抗体阳性率和GMC差异有统计学意义(χ^(2)=687.192,P<0.001;F=174.734,P<0.001)。不同地区麻疹IgG抗体以及GMC差异有统计学意义(χ^(2)=72.545,P<0.001;F=19.946,P<0.001)。结论江苏省健康人群的麻疹抗体阳性率较高,发生大范围暴发或流行的风险较低,但个别地区个别年龄组人群抗体阳性率较低,需加强免疫。结合江苏省现有麻疹发病情况,不需调整现有免疫策略。

关键词：麻疹抗体水平监测 IgG抗体阳性率

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表观遗传修饰调控哮喘发生的研究进展

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生命的化学 2023年第8期43卷 1229-1236页

作者：卢佩珊李佳宁欧阳岁东广东医科大学广东省医学分子诊断重点实验室东莞523808 广东医科大学医学技术学院东莞523808 东莞市寮步医院东莞523430

哮喘是由多种细胞以及细胞组分介导的慢性气道炎症性疾病。其发病率较高、发病时间长且病情反复发作,不容易治愈,已严重影响人们的身体健康。哮喘的发病机制复杂,迄今仍未阐明。近年来,作为一种调控基因变化的方式,表观遗传修饰介导的... 详细信息

哮喘是由多种细胞以及细胞组分介导的慢性气道炎症性疾病。其发病率较高、发病时间长且病情反复发作,不容易治愈,已严重影响人们的身体健康。哮喘的发病机制复杂,迄今仍未阐明。近年来,作为一种调控基因变化的方式,表观遗传修饰介导的基因调控过程与哮喘发病的复杂病理生理变化过程密切相关,是最近被认为影响哮喘发病和异质性的重要机制之一。因此,本文将通过表观遗传修饰的三种主要方式:DNA甲基化、组蛋白修饰和microRNA的异常表达来介绍其在调控哮喘发生中的研究进展,以期为进一步研究哮喘的表观遗传修饰调控机制提供新的思路,以及发现新的哮喘生物标记物和治疗靶点提供参考。

关键词：表观遗传哮喘 DNA甲基化组蛋白修饰 microRNA

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ICU转出患者家属转科应激现状及影响因素的研究

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国际医药卫生导报 2022年第18期28卷 2595-2599页

作者：卢沛珊廖游玩陈颖高晶谢银均广东省人民医院老年重症医学科广州510000

目的识别ICU转出患者家属转科应激的现状及影响因素。方法本研究采用横断面研究方法。选择2020年4月至2021年2月在广东省人民医院ICU治疗后成功转出ICU的120例患者家属为研究对象,其中男性53例、女性67例,年龄为(45.64±9.76)岁。采用... 详细信息

目的识别ICU转出患者家属转科应激的现状及影响因素。方法本研究采用横断面研究方法。选择2020年4月至2021年2月在广东省人民医院ICU治疗后成功转出ICU的120例患者家属为研究对象,其中男性53例、女性67例,年龄为(45.64±9.76)岁。采用一般情况调查表及中文版ICU患者家属迁移应激量表,对其迁移应激状况进行调查。对影响转出ICU后其家属迁移应激状况的因素采用单因素及多因素分析进行分析。统计学方法采用独立样本t检验、方差分析、多元线性回归分析。结果ICU转出患者家属转科应激总分为(54.25±6.31)分,条目均分为(3.23±0.48)分。条目均分最高及最低的维度分别为转科准备[(3.78±0.51)分]、转科满意度[(2.75±0.31)分]。单因素分析显示:家属转科应激得分在不同家属性别、文化程度、宗教信仰及患者年龄、付费方式、ICU治疗时间之间差异均有统计学意义(均P<0.05)。多因素分析显示:影响家属转科应激的主要因素有家属文化程度、家属性别、患者年龄、患者ICU治疗时间(均P<0.05)。结论ICU转出患者家属转科应激水平处于中等偏上。护士在重视患者疾病恢复的同时,也应多关注家属的心理,特别是年龄较小、ICU住院时间较长患者的家属及学历较低的女性家属,给予积极的心理疏导。

关键词： ICU 患者家属转科应激迁移应激

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