Laccase/caffeic acid-catalyzed crosslinking coupled with galactomannan alters the conformational structure of ovalbumin and alleviates Th2-mediated allergic asthma

作     者：Ishfaq Ahmed Suidong Ouyang Shengquan Wu Haochang Song Miaoyuan Zhang Renxing Luo Peishan Lu Jiaqi Deng Tingting Zheng Yanyan Wang Xinguang Liu Gonghua Huang Ishfaq Ahmed;Suidong Ouyang;Shengquan Wu;Haochang Song;Miaoyuan Zhang;Renxing Luo;Peishan Lu;Jiaqi Deng;Tingting Zheng;Yanyan Wang;Xinguang Liu;Gonghua Huang

作者机构：Guangdong Provincial Key Laboratory of Medical Molecular DiagnosticsThe First Dongguan Affiliated HospitalGuangdong Medical UniversityDongguan 523808China Haide CollegeOcean University of ChinaLaoshan CampusQingdao 266100China School of Medical TechnologyGuangdong Medical UniversityDongguan 523808China 

出 版 物：《Food Science and Human Wellness》 (食品科学与人类健康（英文）)

年 卷 期：2024年第13卷第4期

页      面：1962-1973页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 1002[医学-临床医学] 08[工学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 083201[工学-食品科学] 10[医学] 

基　　金：supported by the Guangdong Basic and Applied Basic Research Foundation(2021B1515130004 2021B1515140021) the Scientific Research Start-up Funding of Guangdong Medical University(1026/4SG21229G) China Postdoctoral Science Foundation(2021M702781) Guangdong Medical University Post-doctoral Research Funding(2BH19006P) 

主　　题：Ovalbumin Laccase Galactomannan Conformational structure Asthma 

摘      要：Ovalbumin(OVA)is the major allergenic protein that can induce T helper 2(Th2)-allergic reactions,for which current treatment options are *** this study,we developed a polymerized hypoallergenic OVA product via laccase/caffeic acid(Lac/CA)-catalyzed crosslinking in conjunction with galactomannan(Man).The formation of high molecular weight crosslinked polymers and the Ig G-binding were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE)and Western *** study indicated that Lac/CA-catalyzed crosslinking plus Man conjugation substantially altered secondary and tertiary structures of OVA along with the variation in surface *** digestion stability assay indicated that crosslinked OVA exhibited less resistance in simulated gastric fluid(SGF)and simulated intestinal fluid(SIF).Mouse model study indicated that Lac-Man/OVA ameliorated eosinophilic airway inflammatory response and efficiently downregulated the expression of Th2-related cytokines(interleukin(IL)-4,IL-5,and IL-13),and upregulated IFN-γand IL-10 *** of bone marrow-derived dendritic cells with Lac-Man/OVA suppressed the expression of phenotypic maturation markers(CD80 and CD86)and MHC class II molecules,and suppressed the expression levels of proinflammatory *** knowledge obtained in the present study offers an effective way to acquire a hypoallergenic OVA product that can have a therapeutic effect in alleviating OVA-induced allergic asthma.