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High-yield and rapid isolation of extracellular vesicles by flocculation via orbital acoustic trapping:FLOAT

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Microsystems & Nanoengineering 2024年第1期10卷 313-323页

作者： Joseph Rufo peiran zhang Zeyu Wang Yuyang Gu Kaichun Yang Joseph Rich Chuyi Chen Ruoyu Zhong Ke Jin Ye He Jianping Xia Ke Li Jiarong Wu Yingshi Ouyang Yoel Sadovsky Luke P.Lee Tony Jun Huang Department of Mechanical Engineering and Materials Science Duke UniversityDurhamNCUSA Department of Biomedical Engineering Duke UniversityDurhamNCUSA Department of Obstetrics Gynecologyand Reproductive SciencesMagee-Womens Research InstituteUniversity of PittsburghPittsburghPAUSA Department of Microbiology and Molecular Genetics School of MedicineUniversity of PittsburghPittsburghPAUSA Renal Division and Division of Engineering in Medicine Department of MedicineBrigham and Women’s HospitalHarvard Medical SchoolBostonMAUSA Department of Bioengineering Department of Electrical Engineering and Computer ScienceUniversity of CaliforniaBerkeleyBerkeleyCAUSA Department of Biophysics Institute of Quantum BiophysicsSungkyunkwan UniversitySuwonKorea

Extracellular vesicles(EVs)have been identified as promising biomarkers for the noninvasive diagnosis of various ***,challenges in separating EVs from soluble proteins have resulted in variable EV recovery rates and low ***,we report a high-yield(>90%)and rapid(<10 min)EV isolation method called FLocculation via Orbital Acoustic Trapping(FLOAT).The FLOAT approach utilizes an acoustofluidic droplet centrifuge to rotate and controllably heat liquid *** adding a thermoresponsive polymer flocculant,nanoparticles as small as 20 nm can be rapidly and selectively concentrated at the center of the *** demonstrate the ability of FLOAT to separate urinary EVs from the highly abundant Tamm-Horsfall protein,addressing a significant obstacle in the development of EV-based liquid *** to its high-yield nature,FLOAT reduces biofluid starting volume requirements by a factor of 100(from 20 mL to 200µL),demonstrating its promising potential in point-of-care diagnostics.

关键词： trapping utilize separating

Acoustofluidics for simultaneous nanopartide-based drug loading and exosome encapsulation

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Microsystems & Nanoengineering 2022年第2期8卷 149-159页

作者： Zeyu Wang Joseph Rich Nanjing Hao Yuyang Gu Chuyi Chen Shujie Yang peiran zhang Tony Jun Huang Department of Mechanical Engineering and Materials Science Duke UniversityDurhamNC 27708USA Department of Biomedical Engineering Duke UniversityDurhamNC 27708USA

Nanocarrier and exosome encapsulation has been found to significantly increase the efficacy of targeted drug delivery while also minimizing unwanted side ***,the development of exosome-encapsulated drug nanocarriers is limited by low drug loading efficiencies and/or complex,time-consuming drug loading ***,we have developed an acoustofluidic device that simultaneously performs both drug loading and exosome *** synergistically leveraging the acoustic radiation force,acoustic microstreaming,and shear stresses in a rotating droplet,the concentration,and fusion of exosomes,drugs,and porous silica nanoparticles is *** final product consists of drug-loaded silica nanocarriers that are encased within an exosomal *** drug loading efficiency is significantly improved,with nearly 30%of the free drug(e.g.,doxorubicin)molecules loaded into the ***,this acoustofluidic drug loading system circumvents the need for complex chemical modification,allowing drug loading and encapsulation to be completed within a matter of *** exosome-encapsulated nanocarriers exhibit excellent efficiency in intracellular transport and are capable of significantly inhibiting tumor cell *** utilizing physical forces to rapidly generate hybrid nanocarriers,this acoustofluidic drug loading platform wields the potential to significantly impact innovation in both drug delivery research and applications.

关键词： exosome loading simultaneous

皮肤纤维化疾病中肌成纤维细胞来源的研究进展

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皮肤性病诊疗学杂志 2024年第5期31卷 340-346页

作者：朱显忠麦佩君韦阳海魏茂云邱创烨张沛然齐庆广州医科大学附属第二医院广东广州510260 广州医科大学附属第二医院变态反应(过敏)中心广东广州510260

典型的皮肤纤维化疾病包括增生性瘢痕、瘢痕疙瘩、硬皮病等。该类疾病以肌成纤维细胞过度激活和细胞外基质沉积为特征,可导致永久性瘢痕和器官功能损伤,严重影响患者身心健康。肌成纤维细胞在皮肤纤维化疾病中起到了核心作用。目前研究... 详细信息

典型的皮肤纤维化疾病包括增生性瘢痕、瘢痕疙瘩、硬皮病等。该类疾病以肌成纤维细胞过度激活和细胞外基质沉积为特征,可导致永久性瘢痕和器官功能损伤,严重影响患者身心健康。肌成纤维细胞在皮肤纤维化疾病中起到了核心作用。目前研究表明,成纤维细胞、内皮细胞、周细胞、巨噬细胞等细胞可在经历促纤维化的介质等多因素的诱导、皮肤纤维化相关信号通路的激活、形态学和生化特征的改变、α-平滑肌肌动蛋白(α-SMA)等肌成纤维细胞标记物的表达等过程后,转化为肌成纤维细胞。靶向这些细胞转化的过程有望成为调控皮肤纤维化疾病进展的新治疗策略。本文重点围绕皮肤纤维化疾病中肌成纤维细胞来源的相关进展作一综述。

关键词：肌成纤维细胞皮肤纤维化肌成纤维细胞来源

Delicate chemical structure regulation of nonfullerene acceptor for efficient and large thickness organic solar cells

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Science China Chemistry 2024年第5期67卷 1596-1604页

作者： Zhe zhang Wanying Feng Yunxin zhang Shaohui Yuan Yuyang Bai peiran Wang Zhaoyang Yao Chenxi Li Tainan Duan Xiangjian Wan Bin Kan Yongsheng Chen State Key Laboratory and Institute of Elemento-Organic Chemistry Frontiers Science Center for New Organic MatterThe Centre of Nanoscale Science and Technology and Key Laboratory of Functional Polymer MaterialsRenewable Energy Conversion and Storage Center(RECAST)College of ChemistryNankai UniversityTianjin 300071China School of Materials Science and Engineering National Institute for Advanced MaterialsNankai UniversityTianjin 300350China Chongqing Institute of Green and Intelligent Technology Chongqing SchoolUniversity of Chinese Academy of Sciences(UCAS Chongqing)Chinese Academy of SciencesChongqing 400722China

Inspired by the success of CH-series acceptors, a small-molecular acceptor, CH-Tz was reported by adopting a new conjugationextended electron-deficient unit([1,2,5]thiadiazolo[3,4-b]pyrazine) on the central core. Owing to the enhanced inter-/intramolecular interactions, CH-Tz exhibited near-infrared absorption and an effective three-dimensional molecular packing network in its single crystal. When blended with polymer donor PM6, the binary device achieved a high power conversion efficiency(PCE) of 18.54%, with a notable short-circuit current density(J_(sc)) of 27.54 m A cm-2and an excellent fill factor(FF) over 80%,which can be partly ascribed to the balanced charge transport properties in the blend film. After employing D18-Cl as the third component, an enhanced PCE of 18.85% was achieved due to a more obvious fiber network. Impressively, the CH-Tz-based OSC devices show excellent thermal stability and thickness insensitivity. Record-breaking Jscof 28.92 m A cm-2was reached for PM6:D18-Cl:CH-Tz ternary device with a thickness of 560 nm. Besides, CH-Tz shows potential in fabricating multicomponent high-performance organic solar cells, where over 19% efficiency could be realized in the quaternary device. Our work advances the strong influence of electron-deficient central units on molecular photovoltaic properties and guides the design of acceptors for stable and large-thickness organic solar cells.

关键词： organic solar cells non-fullerene acceptor central unit large thickness molecular packing

CaPtAs: A new noncentrosymmetric superconductor

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Science China(Physics,Mechanics & Astronomy) 2020年第3期63卷 98-105页

作者： Wu Xie peiran zhang Bin Shen WenBing Jiang GuiMing Pang Tian Shang Chao Cao Michael Smidman HuiQiu Yuan Center for Correlated Matter and Department of Physics Zhejiang UniversityHangzhou 310058China Physik-Institut Universitat ZürichZurich CH-8057Switzerland Department of Physics Hangzhou Normal UniversityHangzhou 310036China Collaborative Innovation Center of Advanced Microstructures Nanjing UniversityNanjing 210093China

We report the discovery of a new noncentrosymmetric superconductor *** crystallizes in a tetragonal structure(space group I41md,No.109),featuring three dimensional honeycomb networks of Pt-As and a much elongated c-axis(a=b=4.18?,and c=43.70?).The superconductivity of CaPtAs with Tc=1.47 K was characterized by means of electrical resistivity,specific heat,and ac magnetic *** electronic specific heat Ce(T)/T shows evidence for a deviation from the behavior of a conventional BCS superconductor,and can be reasonably fitted by a p-wave *** upper critical fieldμ0Hc2 of CaPtAs exhibits a moderate anisotropy,with an in-plane value of around 204 mT and an out-of-plane value of 148 *** functional theory calculations indicate that the Pt-5 d and As-4 p orbitals mainly contribute to the density of states near the Fermi level,showing that the Pt-As honeycomb networks may significantly influence the superconducting properties.

关键词： noncentrosymmetric superconductivity CaPtAs

Endothelial Notch activation promotes neutrophil transmigration via downregulating endomucin to aggravate hepatic ischemia/reperfusion injury

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Science China(Life Sciences) 2020年第3期63卷 375-387页

作者： peiran zhang Kangyi Yue Xinli Liu Xianchun Yan Ziyan Yang Juanli Duan Congcong Xia Xinyuan Xu Mei zhang Liang Liang Lin Wang Hua Han State Key Laboratory of Cancer Biology Fourth Military Medical UniversityXi’an 710032China Department of Biochemistry and Molecular Biology Fourth Military Medical UniversityXi’an 710032China Department of Hepatobiliary Surgery Xijing HospitalFourth Military Medical UniversityXi’an 710032China

Inflammatory leukocytes infiltration is orchestrated by mechanisms involving chemokines,selectins,addressins and other adhesion molecules derived from endothelial cells(ECs),but how they respond to inflammatory cues and coordinate leukocyte transmigration remain *** this study,using hepatic ischemia/reperfusion injury(HIRI)as a model,we identified that endothelial Notch activation was rapidly and dynamically induced in liver sinusoidal endothelial cells(LSECs)in acute *** mice with EC-specific Notch activation(NICeCA),HIRI induced exacerbated liver ***,endothelial Notch activation enhanced neutrophil infiltration and tumor necrosis factor(TNF)-αexpression in *** analysis and further qRT-PCR as well as immunofluorescence indicated that endomucin(EMCN),a negative regulator of leukocyte adhesion,was downregulated in LSECs from NICeCA *** was downregulated during HIRI in wild-type mice and in vitro cultured ECs insulted by hypoxia/re-oxygenation *** activation in ECs led to increased neutrophil adhesion and transendothelial migration,which was abrogated by EMCN overexpression in *** mice deficient of RBPj,the integrative transcription factor of canonical Notch signaling,although overwhelming sinusoidal malformation aggravated HIRI,the expression of EMCN was upregulated;and pharmaceutical Notch blockade in vitro also upregulated EMCN and inhibited transendothelial migration of *** Notch activation-exaggerated HIRI was compromised by blocking LFA-1,which mediated leukocyte adherence by associating with ***,endothelial Notch signaling controls neutrophil transmigration via EMCN to modulate acute inflammation in HIRI.

关键词： leukocyte transendothelial migration hepatic ischemia/reperfusion injury(HIRI) liver sinusoidal endothelial cells(LSECs) Notch endomucin(EMCN)

OsPRMT6a-mediated arginine methylation of OsJAZ1 regulates jasmonate signaling and spikelet development in rice

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Molecular Plant 2024年第6期17卷 900-919页

作者： Kun Dong Fuqing Wu Siqi Cheng Shuai Li Feng zhang Xinxin Xing Xin Jin Sheng Luo Miao Feng Rong Miao Yanqi Chang Shuang zhang Xiaoman You peiran Wang Xin zhang Cailin Lei Yulong Ren Shanshan Zhu Xiuping Guo Chuanyin Wu Dong-Lei Yang Qibing Lin Zhijun Cheng Jianmin Wan State Key Laboratory of Crop Gene Resources and Breeding National Key Facility for Crop Gene Resources and Genetic ImprovementInstitute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China State Key Laboratory for Crop Genetics and Germplasm Enhancement Nanjing Agricultural UniversityNanjing 210095China

Although both protein arginine methylation(PRMT)and jasmonate(JA)signaling are crucial for regulating plant development,the relationship between these processes in the control of spikelet development remains *** this study,we used the CRISPR/Cas9 technology to generate two OsPRMT6a loss-of-function mutants that exhibit various abnormal spikelet ***,we found that OsPRMT6a can methylate arginine residues in JA signal repressors OsJAZ1 and *** showed that arginine methylation of OsJAZ1 enhances the binding affinity of OsJAZ1 with the JA receptors OsCOI1a and OsCOI1b in the presence of JAs,thereby promoting the ubiquitination of OsJAZ1 by the SCF^(OsCOI1a/OsCOI1b) complex and degradation via the 26S *** process ultimately releases OsMYC2,a core transcriptional regulator in the JA signaling pathway,to activate or repress JA-responsive genes,thereby maintaining normal plant(spikelet)***,in the osprmt6a-1 mutant,reduced arginine methylation of OsJAZ1 impaires the interaction between OsJAZ1 and OsCOI1a/OsCOI1b in the presence of *** a result,OsJAZ1 proteins become more stable,repressing JA responses,thus causing the formation of abnormal spikelet ***,we discovered that JA signaling reduces the OsPRMT6a mRNA level in an OsMYC2-dependent manner,thereby establishing a negative feedback loop to balance JA *** further found that OsPRMT6a-mediated arginine methylation of OsJAZ1 likely serves as a switch to tune JA signaling to maintain normal spikelet development under harsh environmental conditions such as high ***,our study establishes a direct molecular link between arginine methylation and JA signaling in rice.

关键词： protein arginine methylation jasmonate signaling negative feedback loop spikelet development adaptation to heat stress rice

Floating Escherichia coli by Expressing Cyanobacterial Gas Vesicle Genes

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Journal of Ocean University of China 2015年第1期14卷 84-88页

作者： WANG Tianhe KANG Li LI Jiaheng WU Wenjie zhang peiran GONG Minghao LAI Weihong zhang Chunyan CHANG Lei PENG Yong YANG Zhongzhou LI Lian BAO Yingying XU Haowen zhang Xiaohua SUI Zhenghong YANG Guanpin WANG Xianghong College of Marine Life Science Ocean University of China School of Medicine and Pharmacy Ocean University of China College of Fisheries Ocean University of China College of Information Science and Engineering Ocean University of China School of Mathematical Sciences Ocean University of China Experimental Teaching Center of Marine Life Sciences Ocean University of China

Gas vesicles are hollow, air-filled polyprotein structures that provide the buoyancy to cells. They are found in a variety of prokaryotes. In this study, we isolated a partial gas vesicle protein gene cluster containing gvp A and gvp C20Ψ from Planktothrix rubescens, and inserted it into an expression vector and expressed it in E. coli. The gas vesicle was developed in bacterial cells, which made bacterial cells to float on medium surface. We also amplified gvp A and gvp C20Ψ separately and synthesized an artificial operon by fusing these two genes with the standardized gene expression controlling elements of E. coli. The artificial operon was expressed in E. coli, forming gas vesicles and floating bacteria cells. Our findings verified that the whole set of genes and the overall structure of gas vesicle gene cluster are not necessary for developing gas vesicles in bacteria cells. Two genes, gvp A and gvp C20Ψ, of the gas vesicle gene cluster are sufficient for synthesizing an artificial operon that can develop gas vesicles in bacteria cells. Our findings provided a wide range of applications including easing the harvest of cultured microalgae and bacteria, as well as enriching and remediating aquatic pollutants by constructing gas vesicles in their cells.

关键词：大肠杆菌基因簇气体囊泡过表达细菌细胞蓝藻浮动

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乡村振兴背景下忻州炕围画的传承与创新

丝网印刷 2023年第22期 14-16页

作者：孔霞张珮冉晋中信息学院

对现阶段忻州炕围画这一传统文化进行分析,从炕围画的发展历程、传承的意义及打造特色文化创新等方面入手,强化传统文化的传承认知,深入挖掘传统文化的创新策略,真正为忻州炕围画的发展提供参考价值。

关键词：乡村振兴战略炕围画历程传承创新