Floating Escherichia coli by Expressing Cyanobacterial Gas Vesicle Genes

作     者：WANG Tianhe KANG Li LI Jiaheng WU Wenjie ZHANG Peiran GONG Minghao LAI Weihong ZHANG Chunyan CHANG Lei PENG Yong YANG Zhongzhou LI Lian BAO Yingying XU Haowen ZHANG Xiaohua SUI Zhenghong YANG Guanpin WANG Xianghong 

作者机构：College of Marine Life Science Ocean University of China School of Medicine and Pharmacy Ocean University of China College of Fisheries Ocean University of China College of Information Science and Engineering Ocean University of China School of Mathematical Sciences Ocean University of China Experimental Teaching Center of Marine Life Sciences Ocean University of China 

出 版 物：《Journal of Ocean University of China》 (中国海洋大学学报（英文版）)

年 卷 期：2015年第14卷第1期

页      面：84-88页

核心收录：

学科分类：081702[工学-化学工艺] 0908[农学-水产] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 090102[农学-作物遗传育种] 0710[理学-生物学] 0707[理学-海洋科学] 0817[工学-化学工程与技术] 0815[工学-水利工程] 0824[工学-船舶与海洋工程] 0901[农学-作物学] 0836[工学-生物工程] 

基　　金：supported by Undergraduate Institution of Marine Biological Science  Ocean University of China (OUC) 

主　　题：大肠杆菌 基因簇 气体 囊泡 过表达 细菌细胞 蓝藻 浮动 

摘      要：Gas vesicles are hollow, air-filled polyprotein structures that provide the buoyancy to cells. They are found in a variety of prokaryotes. In this study, we isolated a partial gas vesicle protein gene cluster containing gvp A and gvp C20Ψ from Planktothrix rubescens, and inserted it into an expression vector and expressed it in E. coli. The gas vesicle was developed in bacterial cells, which made bacterial cells to float on medium surface. We also amplified gvp A and gvp C20Ψ separately and synthesized an artificial operon by fusing these two genes with the standardized gene expression controlling elements of E. coli. The artificial operon was expressed in E. coli, forming gas vesicles and floating bacteria cells. Our findings verified that the whole set of genes and the overall structure of gas vesicle gene cluster are not necessary for developing gas vesicles in bacteria cells. Two genes, gvp A and gvp C20Ψ, of the gas vesicle gene cluster are sufficient for synthesizing an artificial operon that can develop gas vesicles in bacteria cells. Our findings provided a wide range of applications including easing the harvest of cultured microalgae and bacteria, as well as enriching and remediating aquatic pollutants by constructing gas vesicles in their cells.