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Acoustofluidics for simultaneous nanopartide-based drug loading and exosome encapsulation

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Microsystems & Nanoengineering 2022年第2期8卷 149-159页

作者： Zeyu Wang Joseph Rich Nanjing Hao Yuyang Gu Chuyi Chen Shujie Yang peiran zhang Tony Jun Huang Department of Mechanical Engineering and Materials Science Duke UniversityDurhamNC 27708USA Department of Biomedical Engineering Duke UniversityDurhamNC 27708USA

Nanocarrier and exosome encapsulation has been found to significantly increase the efficacy of targeted drug delivery while also minimizing unwanted side ***,the development of exosome-encapsulated drug nanocarriers is limited by low drug loading efficiencies and/or complex,time-consuming drug loading ***,we have developed an acoustofluidic device that simultaneously performs both drug loading and exosome *** synergistically leveraging the acoustic radiation force,acoustic microstreaming,and shear stresses in a rotating droplet,the concentration,and fusion of exosomes,drugs,and porous silica nanoparticles is *** final product consists of drug-loaded silica nanocarriers that are encased within an exosomal *** drug loading efficiency is significantly improved,with nearly 30%of the free drug(e.g.,doxorubicin)molecules loaded into the ***,this acoustofluidic drug loading system circumvents the need for complex chemical modification,allowing drug loading and encapsulation to be completed within a matter of *** exosome-encapsulated nanocarriers exhibit excellent efficiency in intracellular transport and are capable of significantly inhibiting tumor cell *** utilizing physical forces to rapidly generate hybrid nanocarriers,this acoustofluidic drug loading platform wields the potential to significantly impact innovation in both drug delivery research and applications.

关键词： exosome loading simultaneous

CaPtAs: A new noncentrosymmetric superconductor

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Science China(Physics,Mechanics & Astronomy) 2020年第3期63卷 98-105页

作者： Wu Xie peiran zhang Bin Shen WenBing Jiang GuiMing Pang Tian Shang Chao Cao Michael Smidman HuiQiu Yuan Center for Correlated Matter and Department of Physics Zhejiang UniversityHangzhou 310058China Physik-Institut Universitat ZürichZurich CH-8057Switzerland Department of Physics Hangzhou Normal UniversityHangzhou 310036China Collaborative Innovation Center of Advanced Microstructures Nanjing UniversityNanjing 210093China

We report the discovery of a new noncentrosymmetric superconductor *** crystallizes in a tetragonal structure(space group I41md,No.109),featuring three dimensional honeycomb networks of Pt-As and a much elongated c-axis(a=b=4.18?,and c=43.70?).The superconductivity of CaPtAs with Tc=1.47 K was characterized by means of electrical resistivity,specific heat,and ac magnetic *** electronic specific heat Ce(T)/T shows evidence for a deviation from the behavior of a conventional BCS superconductor,and can be reasonably fitted by a p-wave *** upper critical fieldμ0Hc2 of CaPtAs exhibits a moderate anisotropy,with an in-plane value of around 204 mT and an out-of-plane value of 148 *** functional theory calculations indicate that the Pt-5 d and As-4 p orbitals mainly contribute to the density of states near the Fermi level,showing that the Pt-As honeycomb networks may significantly influence the superconducting properties.

关键词： noncentrosymmetric superconductivity CaPtAs

Endothelial Notch activation promotes neutrophil transmigration via downregulating endomucin to aggravate hepatic ischemia/reperfusion injury

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Science China(Life Sciences) 2020年第3期63卷 375-387页

作者： peiran zhang Kangyi Yue Xinli Liu Xianchun Yan Ziyan Yang Juanli Duan Congcong Xia Xinyuan Xu Mei zhang Liang Liang Lin Wang Hua Han State Key Laboratory of Cancer Biology Fourth Military Medical UniversityXi’an 710032China Department of Biochemistry and Molecular Biology Fourth Military Medical UniversityXi’an 710032China Department of Hepatobiliary Surgery Xijing HospitalFourth Military Medical UniversityXi’an 710032China

Inflammatory leukocytes infiltration is orchestrated by mechanisms involving chemokines,selectins,addressins and other adhesion molecules derived from endothelial cells(ECs),but how they respond to inflammatory cues and coordinate leukocyte transmigration remain *** this study,using hepatic ischemia/reperfusion injury(HIRI)as a model,we identified that endothelial Notch activation was rapidly and dynamically induced in liver sinusoidal endothelial cells(LSECs)in acute *** mice with EC-specific Notch activation(NICeCA),HIRI induced exacerbated liver ***,endothelial Notch activation enhanced neutrophil infiltration and tumor necrosis factor(TNF)-αexpression in *** analysis and further qRT-PCR as well as immunofluorescence indicated that endomucin(EMCN),a negative regulator of leukocyte adhesion,was downregulated in LSECs from NICeCA *** was downregulated during HIRI in wild-type mice and in vitro cultured ECs insulted by hypoxia/re-oxygenation *** activation in ECs led to increased neutrophil adhesion and transendothelial migration,which was abrogated by EMCN overexpression in *** mice deficient of RBPj,the integrative transcription factor of canonical Notch signaling,although overwhelming sinusoidal malformation aggravated HIRI,the expression of EMCN was upregulated;and pharmaceutical Notch blockade in vitro also upregulated EMCN and inhibited transendothelial migration of *** Notch activation-exaggerated HIRI was compromised by blocking LFA-1,which mediated leukocyte adherence by associating with ***,endothelial Notch signaling controls neutrophil transmigration via EMCN to modulate acute inflammation in HIRI.

关键词： leukocyte transendothelial migration hepatic ischemia/reperfusion injury(HIRI) liver sinusoidal endothelial cells(LSECs) Notch endomucin(EMCN)

电磁脉冲攻击下片上配电网络IR Drop分析方法

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计算机辅助设计与图形学学报 2024年第4期36卷 582-590页

作者：刘强张培然天津大学微电子学院天津300072 天津市成像与感知微电子技术重点实验室天津300072

电磁脉冲攻击对集成电路的安全具有很强的威胁性.为了有效地抵御电磁脉冲攻击,针对片上配电网络易受电磁脉冲影响的问题,提出了一种电磁脉冲攻击下片上配电网络IRdrop分布的分析方法.首先,在集成电路布局规划阶段,基于有限元仿真构建片... 详细信息

电磁脉冲攻击对集成电路的安全具有很强的威胁性.为了有效地抵御电磁脉冲攻击,针对片上配电网络易受电磁脉冲影响的问题,提出了一种电磁脉冲攻击下片上配电网络IRdrop分布的分析方法.首先,在集成电路布局规划阶段,基于有限元仿真构建片上配电网络模型和电磁脉冲攻击模型,仿真获得电磁脉冲下配电网络上感应电流密度的分布并计算感应电流,然后将感应电流加载到配电网络模型上,使用IR分析工具分析IRdrop分布.基于TSMC180 nm工艺版图的IR drop分析结果显示,电磁脉冲攻击能够在电源和地网络中引入2.3 V以上的IR drop.与现有基于电流分布理论值的分析方法相比,该分析方法能够更准确地获取电磁脉冲下配电网络中的IR drop分布.该分析方法可用于指导改进配电网络的设计,提升抗电磁脉冲攻击能力.实验结果显示,增加一组供电端口后,电源和地网络中的最大IR drop分别降低了28%和24%.

关键词：集成电路片上配电网络电磁脉冲攻击电压降

Floating Escherichia coli by Expressing Cyanobacterial Gas Vesicle Genes

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Journal of Ocean University of China 2015年第1期14卷 84-88页

作者： WANG Tianhe KANG Li LI Jiaheng WU Wenjie zhang peiran GONG Minghao LAI Weihong zhang Chunyan CHANG Lei PENG Yong YANG Zhongzhou LI Lian BAO Yingying XU Haowen zhang Xiaohua SUI Zhenghong YANG Guanpin WANG Xianghong College of Marine Life Science Ocean University of China School of Medicine and Pharmacy Ocean University of China College of Fisheries Ocean University of China College of Information Science and Engineering Ocean University of China School of Mathematical Sciences Ocean University of China Experimental Teaching Center of Marine Life Sciences Ocean University of China

Gas vesicles are hollow, air-filled polyprotein structures that provide the buoyancy to cells. They are found in a variety of prokaryotes. In this study, we isolated a partial gas vesicle protein gene cluster containing gvpA and gvpC20ψ from Planktothrix rubescens, and inserted it into an expression vector and expressed it in E. coli. The gas vesicle was developed in bacterial cells, which made bacterial cells to float on medium surface. We also amplified gvpA and gvpC20ψ separately and synthesized an artificial operon by fusing these two genes with the standardized gene expression controlling elements of E. coli. The artificial operon was expressed in E. coli, forming gas vesicles and floating bacteria cells. Our findings verified that the whole set of genes and the overall structure of gas vesicle gene cluster are not necessary for developing gas vesicles in bacteria cells. Two genes, gvpA and gvpC20ψ, of the gas vesicle gene cluster are sufficient for synthesizing an artificial operon that can develop gas vesicles in bacteria cells. Our findings provided a wide range of applications including easing the harvest of cultured microalgae and bacteria, as well as enriching and remediating aquatic pollutants by constructing gas vesicles in their ceils.

关键词： gas vesicle gene cluster gas vesicle gene buoyancy

High-yield and rapid isolation of extracellular vesicles by flocculation via orbital acoustic trapping:FLOAT

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Microsystems & Nanoengineering 2024年第1期10卷 313-323页

作者： Joseph Rufo peiran zhang Zeyu Wang Yuyang Gu Kaichun Yang Joseph Rich Chuyi Chen Ruoyu Zhong Ke Jin Ye He Jianping Xia Ke Li Jiarong Wu Yingshi Ouyang Yoel Sadovsky Luke P.Lee Tony Jun Huang Department of Mechanical Engineering and Materials Science Duke UniversityDurhamNCUSA Department of Biomedical Engineering Duke UniversityDurhamNCUSA Department of Obstetrics Gynecologyand Reproductive SciencesMagee-Womens Research InstituteUniversity of PittsburghPittsburghPAUSA Department of Microbiology and Molecular Genetics School of MedicineUniversity of PittsburghPittsburghPAUSA Renal Division and Division of Engineering in Medicine Department of MedicineBrigham and Women’s HospitalHarvard Medical SchoolBostonMAUSA Department of Bioengineering Department of Electrical Engineering and Computer ScienceUniversity of CaliforniaBerkeleyBerkeleyCAUSA Department of Biophysics Institute of Quantum BiophysicsSungkyunkwan UniversitySuwonKorea

Extracellular vesicles(EVs)have been identified as promising biomarkers for the noninvasive diagnosis of various ***,challenges in separating EVs from soluble proteins have resulted in variable EV recovery rates and low ***,we report a high-yield(>90%)and rapid(<10 min)EV isolation method called FLocculation via Orbital Acoustic Trapping(FLOAT).The FLOAT approach utilizes an acoustofluidic droplet centrifuge to rotate and controllably heat liquid *** adding a thermoresponsive polymer flocculant,nanoparticles as small as 20 nm can be rapidly and selectively concentrated at the center of the *** demonstrate the ability of FLOAT to separate urinary EVs from the highly abundant Tamm-Horsfall protein,addressing a significant obstacle in the development of EV-based liquid *** to its high-yield nature,FLOAT reduces biofluid starting volume requirements by a factor of 100(from 20 mL to 200µL),demonstrating its promising potential in point-of-care diagnostics.

关键词： trapping utilize separating

Dielectrophoresis Single Cell Trap-and-release for Raman Activated Cell Sorting

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Dielectrophoresis Single Cell Trap-and-release for Raman Act...

温度对悬臂梁静电驱动RF-MEMS开关性能的影响

第七届全国仪器分析及样品预处理学术研讨会

作者： Bo Ma peiran zhang Xu zhang Yun Wang Lihui Ren Jian Xu Wei Huang Qingdao Institute of BioEnergy and BioProcess Technology ChineseAcademy of Science University of Sheffield Western Bank Sheffield S10 2TN UK

Raman-activated cell sorting （RACS） offers prospects to complement the widely applied fluorescence-activated cell sorting （FACS）. However, the methods used for measuring the Raman spectrum led to acquisition time of seconds to minutes per cell, which is prohibitive for rapid cell sorting applications. The on-demand single cell trap-and-release enables RACSin a flowing modeon the microfluidics device by extending the Raman spectrum acquisition time. In contrast to earlier work using optical tweezers to trap single cell[1, 2],we integrated Dielectrophoresis（DEP）electrode pair into the sorting chip to trap single cell for Raman acquisition. Single Yeast cells was trapped and released serially in 20HZ frequency by controlling the voltage applied on the electrodes *** sorting efficiency was validated by sorting the mixture of 50% yeast cells and polystyrene beads （5 μm） and the yeast concentration was enriched to 90%.

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固体电子学研究与进展 2014年第1期34卷 69-74页

作者：张沛然朱健姜理利南京电子器件研究所南京210016 微波毫米波单片集成和模块电路重点实验室南京210016

介绍了温度对悬臂梁式RF-MEMS开关的影响。以南京电子器件研究所研制的悬臂梁式RF-MEMS开关为实验样品,常温下(25°C)先测定样品的驱动电压和射频特性,再将样品置于温度恒定的烘箱中热烘1h,取出后在常温条件下测定其机械形貌及电学性能... 详细信息

介绍了温度对悬臂梁式RF-MEMS开关的影响。以南京电子器件研究所研制的悬臂梁式RF-MEMS开关为实验样品,常温下(25°C)先测定样品的驱动电压和射频特性,再将样品置于温度恒定的烘箱中热烘1h,取出后在常温条件下测定其机械形貌及电学性能。烘箱的温度从50°C变化到200°C,步进50°C。针对每个温度做一轮实验,最后将所得数据进行对比。实验结果表明,温度对于开关的射频性能影响极其微弱,但驱动电压对于温度却有较强的依赖性。分析认为,当温度变化时,悬臂梁结构的翘曲是影响驱动电压变化的主要因素。最后提出了几种可以提高结构温度稳定性的方法。

关键词：射频-微电子机械系统开关温度悬臂梁驱动电压

高校青年拔尖创新人才的核心特质、成长规律与培养策略

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华南师范大学学报（社会科学版） 2024年第5期 44-56,207页

作者：张沛然李红瑶李广东北师范大学教师教育研究院长春130024 东北师范大学教育学部长春130024

创新是推动经济社会发展的战略支点,拔尖创新人才则是提升国家整体创新水平、推动新质生产力发展的关键因素。高校青年拔尖创新人才作为国家、高校发展的中坚力量,其专业发展是服务国家战略需求的关键路径。高校青年拔尖创新人才具有崇... 详细信息

创新是推动经济社会发展的战略支点,拔尖创新人才则是提升国家整体创新水平、推动新质生产力发展的关键因素。高校青年拔尖创新人才作为国家、高校发展的中坚力量,其专业发展是服务国家战略需求的关键路径。高校青年拔尖创新人才具有崇高的历史使命、融合的多重思维、执着的探索精神三大核心特质,受到环境孵化下的共生效应规律、能力涌现下的积累效应规律以及团队共建下的群体效应规律共同作用。针对高校青年拔尖创新人才的培养,应以引才聚才为目的创设梯次合理、衔接紧密的成长通道,以量才促才为核心制定契合度高、针对性强的评价政策,以容才敬才为追求构建可持续性、高质量发展的激励机制。这一系列举措旨在探索并推进一条兼具中国特色与世界一流水平的高校青年拔尖创新人才自主培育之路。

关键词：青年拔尖创新人才核心特质成长规律培养策略