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Expanding our understanding of marine viral diversity through metagenomic analyses of biofilms

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Marine Life Science & Technology 2021年第3期3卷 395-404页

作者： Wei Ding Ruojun Wang Zhicong Liang Rui Zhang pei-yuan qian Weipeng Zhan College of Marine Life Sciences Ocean University of ChinaQingdao266100China Institute for Advanced Ocean Study Ocean University of ChinaQingdao266100China Department of Ocean Sciences Hong Kong University of Science and TechnologyHong KongChina Fok Ying Tung Research Institute Hong Kong University of Science and TechnologyGuangzhou510000China Department of Mathematics Hong Kong University of Science and TechnologyHong KongChina State Key Laboratory of Marine Environmental Science Xiamen UniversityXiamen361005China

Recent metagenomics surveys have provided insights into the marine ***,these surveys have focused solely on viruses in seawater,neglecting those associated with *** analyzing 1.75 terabases of biofilm metagenomic data,3974 viral sequences were identified from eight locations around the *** 90%of these viral sequences were not found in previously reported *** between biofilm and seawater metagenomes identified viruses that are endemic to the biofilm *** of viral sequences integrated within biofilm-derived microbial genomes revealed potential functional genes for trimeric autotransporter adhesin and polysaccharide metabolism,which may contribute to biofilm formation by the bacterial ***,more than 70%of the genes could not be *** findings show marine biofilms to be a reservoir of novel viruses and have enhanced our understanding of natural virus-bacteria ecosystems.

关键词： Ocean Virus Biofilm Metagenomics

Draft genome of an Aerophobetes bacterium reveals a facultative lifestyle in deep-sea anaerobic sediments

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Science Bulletin 2016年第15期61卷 1176-1186页

作者： Yong Wang Zhao-Ming Gao Jiang-Tao Li Salim Bougouffa Ren Mao Tian Vladimir B. Bajic pei-yuan qian Sanya Institute of Deep Sea Science and Engineering ChineseAcademy of Sciences Sanya 572000 China Division of Life Science Hong Kong University of Science and Technology Clear Water Bay Hong Kong China State Key Laboratory of Marine Geology Tongji UniversityShanghai 200092 China Computational Bioscience Research Center King Abdullah University of Science and Technology Thuwal Jeddah 23955-6900 Saudi Arabia

Aerophobetes （or CD12） is a recently defined bacterial phylum, of which the metabolic processes and ecological importance remain unclear. In the present study, we obtained the draft genome of an Aerophobetes bac- terium TCSI from saline sediment near the Thuwal cold seep in the Red Sea using a genome binning method. Analysis of 16S rRNA genes of TCS1 and close relatives revealed wide distribution of Aerophobetes in deep-sea sediments. Phylogenetic relationships showed affinity between Aerophobetes TCS 1 and some thermophilic bac- terial phyla. The genome of TCS1 （at least 1.27 Mbp） contains a full set of genes encoding core metabolic path- ways, including glycolysis and pyruvate fermentation to produce acetyl-CoA and acetate. The identification of cross-membrane sugar transporter genes further indicates its potential ability to consume carbohydrates preserved inthe sediment under the microbial mat. Aerophobetes bac- terium TCS1 therefore probably carried out saccharolytic and fermentative metabolism. The genes responsible for autotrophic synthesis of acetyl-CoA via the Wood-Ljung- dahl pathway were also found in the genome. Phylogenetic study of the essential genes for the Wood-Ljungdahl pathway implied relative independence of Aerophobetes bacterium from the known acetogens and methanogens. Compared with genomes of acetogenic bacteria, Aero- phobetes bacterium TCS 1 genome lacks the genes involved in nitrogen metabolism, sulfur metabolism, signal trans- duction and cell motility. The metabolic activities of TCS 1 might depend on geochemical conditions such as supplies of CO2, hydrogen and sugars, and therefore the TCSI might be a facultative bacterium in anaerobic saline sedi- ments near cold seeps.

关键词： Deep-sea sediment Aerophobetes Metagenome Sugar fermentation Cold seep

Interactions among deep-sea mussels and their epibiotic and endosymbiotic chemoautotrophic bacteria: Insights from multi-omics analysis

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Zoological Research 2023年第1期44卷 106-125页

作者： Yi-Tao Lin Ting Xu Jack Chi-Ho Ip Yanan Sun Ling Fang Tiangang Luan Yu Zhang pei-yuan qian Jian-Wen Qiu Department of Biology Hong Kong Baptist UniversityHong Kong SARChina Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou) GuangzhouGuangdong 511458China Department of Ocean Science Hong Kong University of Science and TechnologyHong Kong SARChina Instrumental Analysis&Research Center Sun Yat-Sen UniversityGuangzhouGuangdong 510875China State Key Laboratory of Biocontrol School of Life SciencesSun Yat-Sen UniversityGuangzhouGuangdong 510875China Institute of Environmental and Ecological Engineering Guangdong University of TechnologyGuangzhouGuangdong 510006China College of Life Sciences and Oceanography Shenzhen UniversityShenzhenGuangdong 518060China

Endosymbiosis with Gammaproteobacteria is fundamental for the success of bathymodioline mussels in deep-sea chemosynthesis-based ecosystems. However, the recent discovery of Campylobacteria on the gill surfaces of these mussels suggests that these host-bacterial relationships may be more complex than previously thought. Using the cold-seep mussel(Gigantidas haimaensis) as a model, we explored this hostbacterial system by assembling the host transcriptome and genomes of its epibiotic Campylobacteria and endosymbiotic Gammaproteobacteria and quantifying their gene and protein expression levels. We found that the epibiont applies a sulfur oxidizing(SOX)multienzyme complex with the acquisition of sox B from Gammaproteobacteria for energy production and switched from a reductive tricarboxylic acid (rTCA) cycle to a Calvin-Benson-Bassham(CBB)cycle for carbon assimilation. The host provides metabolic intermediates, inorganic carbon, and thiosulfate to satisfy the materials and energy requirements of the epibiont, but whether the epibiont benefits the host is unclear. The endosymbiont adopts methane oxidation and the ribulose monophosphate pathway(Ru MP) for energy production, providing the major source of energy for itself and the host. The host obtains most of its nutrients, such as lysine, glutamine, valine,isoleucine, leucine, histidine, and folate, from the endosymbiont. In addition, host pattern recognition receptors, including toll-like receptors, peptidoglycan recognition proteins, and C-type lectins, may participate in bacterial infection, maintenance, and population regulation. Overall, this study provides insights into the complex host-bacterial relationships that have enabled mussels and bacteria to thrive in deep-sea chemosynthetic ecosystems.

关键词： Bathymodioline Chemosynthesis Cold seep Deep-sea adaptation Symbiosis

Regulatory Role of Retinoic Acid in Male Pregnancy of the Seahorse

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The Innovation 2020年第3期1卷 72-80页

作者： Chunyan Li Yongxin Li Geng Qin Zelin Chen Meng Qu Bo Zhang Xue Han Xin Wang pei-yuan qian qiang Lin CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology South China Sea Institute of OceanologyInnovation of South China Sea Ecology and Environmental EngineeringChinese Academy of Sciences510301 GuangzhouChina Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou) 511458 GuangzhouChina Department of Chemistry The University of Hong KongPokfulamHong KongHong Kong SARChina Department of Ocean Science and Hong Kong Branch of Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou) Hong Kong University of Science and TechnologyKowloonHong KongChina

Seahorses epitomize the exuberance of *** have the unique characteristic of male pregnancy,which includes the carrying of many embryos in a brood pouch that incubates and nourishes the embryos,similar to the mammalian ***,the regulatory networks underlying brood pouch formation and pregnancy remain largely *** this study,comparative transcriptomic and metabolomic profiling on the lined seahorse Hippocampus erectus,with unformed,newly formed,and pregnant brood pouches identified a total of 141 and 2,533 differentially expressed genes together with 73 and 121 significantly differential metabolites related to brood pouch formation and pregnancy,***,integrative omics analysis revealed that retinoic acid(RA)synthesis and signaling pathway played essential roles in the formation of the brood pouch and *** might function upstream of testosterone and progesterone,thereby directly influencing brood pouch formation by regulating the expression of fshr and *** results also revealed that RA regulates antioxidant defenses,particularly during male ***,pregnancy caused a consistent decrease in RA,canthaxanthin,astaxanthin,and glutathione synthetase,and an increase in susceptibility to oxidative stress,which may balance brood pouch development and reproduction in seahorses and pave the way to successful gestation.

关键词： SEAHORSE MALE PREGNANCY BROOD POUCH INTEGRATIVE OMICS RETINOIC ACID ANTIOXIDANT DEFENSE

Low temperature magnetism in the rare-earth perovskite GdScO3

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Chinese Physics B 2020年第5期29卷 47-51页

作者： Jie-Ming Sheng Xu-Cai Kan Han Ge pei-qian yuan Lei Zhang Nan Zhao Zong-Mei Song yuan-Yin Yao Ji-Ning Tang Shan-Min Wang Ming-Liang Tian Xin Tong Liu-Suo Wu Department of Physics Southern University of Science and TechnologyShenzhen 518055China Institute of High Energy Physics Chinese Academy of Sciences(CAS)Beijing 100049China Spallation Neutron Source Science Center Dongguan 523803China School of Physics and Materials Science Anhui UniversityHefei 230601China High Magnetic Field Laboratory Chinese Academy of Science(CAS)Hefei 230031China

The magnetic phase diagram of rare-earth perovskite compound,GdScO3,has been investigated by magnetization and heat *** system undergoes an antiferromagnetic phase transition at TN=2.6 K,with an easy axis of magnetization along the a *** magnetization measurements show that it exists a spin-flop transition around 0.3 T for the applied field along the a *** critical magnetic field for the antiferromagnetic-to-paramagnetic transition is near 3.2 T when temperature approaches *** scaling susceptibilities,we presume this point(B=3.2 T,T=0 K)might be a fieldinduced quantum critical point and the magnetic critical fluctuations can even be felt above TN.

关键词： rare-earth perovskite magnetization spin-flop transition quantum critical point

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辨证治疗肠易激综合征76例

北京中医药大学学报（中医临床版） 2007年第2期14卷 14-15页

作者：原培谦长治市中医研究所附属医院山西046000

目的观察中医辨证治疗肠易激综合征(IBS)的临床疗效。方法对76例符合诊断标准的患者依据四诊所得辨证分为4型(肝郁脾虚、气滞肠涩、脾虚湿困和脾肾阳虚),予中药治疗。结果除脾肾阳虚型外,其他三型均有较为满意的临床疗效。结论辨证分型... 详细信息

目的观察中医辨证治疗肠易激综合征(IBS)的临床疗效。方法对76例符合诊断标准的患者依据四诊所得辨证分为4型(肝郁脾虚、气滞肠涩、脾虚湿困和脾肾阳虚),予中药治疗。结果除脾肾阳虚型外,其他三型均有较为满意的临床疗效。结论辨证分型治疗是目前治疗IBS较好的方法。

关键词：肠易激综合征辨证治疗疏肝理气

Direct and rapid detection of serum amino acid and monoamine neurotransmitters to assist the diagnosis of panic disorder

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Science China Chemistry 2025年第01期 377-384页

作者： Shiwen Liu Xuerui Wan Meng Zhao Jiaqi Wang Weilan Wu Linlin You Yonggui yuan qian Xu Rong Gao Key Laboratory of Environmental Medicine Engineering Ministry of Education School of Public Health Southeast University Key Laboratory of Modern Toxicology of Ministry of Education Department of Hygienic Analysis and Detection School of Public HealthNanjing Medical University Department of Psychosomatics and Psychiatry Zhongda Hospital School of Medicine Southeast University

The measurement of amino acid and monoamine neurotransmitters（NTs） in serum plays a central role in the identification and monitoring of clinical panic disorder（PD）. However, the strong polarity and chemical heterogeneity of NTs make direct extraction and rapid detection extremely challenging. Herein, we developed a strategy that integrated solid phase extraction based on magnetic Fe3O4coated with polydopamine（Fe3O4@PDA） and direct analysis of mass spectrometry in real time（DART-MS） for quantification of 10 NTs. We systematically investigated the optimal conditions of this strategy to achieve good accuracy and precision. Compared with existing magnetic solid phase extraction methods for NTs detection in biological samples, this strategy could adsorb more targets while having similar adsorption efficiencies, and the total process time was reduced by an average of 69.42%. We rapidly determined the metabolic abnormality of 6 types of NTs in the serum of PD patients and demonstrated their diagnostic accuracy. In addition, an outstanding feature of PD in which tryptophan was more metabolized by the KYN pathway and the 5-hydroxytryptamine pathway was inhibited could be elucidated by this strategy. Our results highlighted the potential ability of Fe3O4@PDA coupled with DART-MS to assist in the clinical diagnosis of PD.

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Release and uptake of nutrients during incubation of Merionecta rubra(Mesodinium rubrum)from a red tide under nutrient addition experiment

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Release and uptake of nutrients during incubation of Merione...

Low-Temperature Fabrication of Stable Black-Phase CsPbI₃ Perovskite Flexible Photodetectors Toward Wearable Health Monitoring

中国科学院海洋科学青年学术研讨会暨2001年海洋湖沼科学青年学者论坛

作者： Xiuxian Song Kedong Yin pei-yuan qian ZhimingYuMadeline C.S.WuDepartment of BiologyCenter for Coastal and Atmospheric Research Hong Kong University of Science and Technology Kowloon, Hong KongInstitute of Oceanography, Chinese Academic of Sciences Qingdao, 266071 China

A red tide of symbiotic algae-ciliate Merionecta rubra(previously Mesodinium rubrum)occurred on December 1,2000 in Port Shelter of Hong *** was very high(63.7μgL )in the red tide patch,but was only<2μg Lin the non-red-tide *** only 0.58μM in the red tide patch vs～5.27μM in the non-red-tide *** ofNH,POand SiOwere similar between the red tide patch and other ***

关键词： Merionecta rubra red tide patch reference group nutnents uptake and release nutrient addition

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NANO-MICRO LETTERS 2025年第1期17卷 1-14页

作者： Zhao, Yingjie Sun, Yicheng pei, Chaoxin Yin, Xing Li, Xinyi Hao, Yi Zhang, Mengru yuan, Meng Zhou, Jinglin Chen, Yu Song, Yanlin Zhengzhou Univ Coll Chem Zhengzhou 450001 Peoples R China Zhengzhou Univ Pingyuan Lab Zhengzhou 450001 Peoples R China Chinese Acad Sci Inst Chem Key Lab Green Printing Beijing 100190 Peoples R China Chinese Acad Sci Tech Inst Phys & Chem Key Lab Bioinspired Mat & Interfacial Sci Beijing 100190 Peoples R China Chinese Acad Sci Inst High Energy Phys Beijing 100049 Peoples R China

Low-temperature fabrication of black-phase CsPbI3 perovskite films is first demonstrated by using diphenylphosphinic chloride additive under 30-50 degrees C, arising from the steric effect and chloride insertion ***-area high-quality all-inorganic perovskite films with fewer defects enhanced crystallographic orientation, and excellent environmental stability is *** record performances are demonstrated for flexible wearable photodetectors with a responsivity of 42.1 A W-1, a detectivity of 1.3 x 1014 Jones, high-fidelity image, photoplethysmography sensor functions, and high mechanical stability. Flexible wearable optoelectronic devices fabricated from organic-inorganic hybrid perovskites significantly accelerate the development of portable energy, biomedicine, and sensing fields, but their poor thermal stability hinders further applications. Conversely, all-inorganic perovskites possess excellent thermal stability, but black-phase all-inorganic perovskite film usually requires high-temperature annealing steps, which increases energy consumption and is not conducive to the fabrication of flexible wearable devices. In this work, an unprecedented low-temperature fabrication of stable black-phase CsPbI3 perovskite films is demonstrated by the in situ hydrolysis reaction of diphenylphosphinic chloride additive. The released diphenyl phosphate and chloride ions during the hydrolysis reaction significantly lower the phase transition temperature and effectively passivate the defects in the perovskite films, yielding high-performance photodetectors with a responsivity of 42.1 A W-1 and a detectivity of 1.3 x 1014 Jones. Furthermore, high-fidelity image and photoplethysmography sensors are demonstrated based on the fabricated flexible wearable photodetectors. This work provides a new perspective for the low-temperature fabrication of large-area all-inorganic perovskite flexible optoelectronic devices.

关键词： In situ hydrolyzation Low-temperature processing All-inorganic perovskite Flexible photodetectors Health monitoring