Interactions among deep-sea mussels and their epibiotic and endosymbiotic chemoautotrophic bacteria: Insights from multi-omics analysis

作     者：Yi-Tao Lin Ting Xu Jack Chi-Ho Ip Yanan Sun Ling Fang Tiangang Luan Yu Zhang Pei-Yuan Qian Jian-Wen Qiu Yi-Tao Lin;Ting Xu;Jack Chi-Ho Ip;Yanan Sun;Ling Fang;Tiangang Luan;Yu Zhang;Pei-Yuan Qian;Jian-Wen Qiu

作者机构：Department of BiologyHong Kong Baptist UniversityHong Kong SARChina Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou)GuangzhouGuangdong 511458China Department of Ocean ScienceHong Kong University of Science and TechnologyHong Kong SARChina Instrumental Analysis&Research CenterSun Yat-Sen UniversityGuangzhouGuangdong 510875China State Key Laboratory of BiocontrolSchool of Life SciencesSun Yat-Sen UniversityGuangzhouGuangdong 510875China Institute of Environmental and Ecological EngineeringGuangdong University of TechnologyGuangzhouGuangdong 510006China College of Life Sciences and OceanographyShenzhen UniversityShenzhenGuangdong 518060China 

出 版 物：《Zoological Research》 (动物学研究（英文）)

年 卷 期：2023年第44卷第1期

页      面：106-125页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071002[理学-动物学] 

基　　金：supported by the Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou)(GML2019ZD0409,SMSEGL20SC02) Research Grants Council of Hong Kong(12101021) Guangdong Natural Science Foundation(2020A1515011117) 

主　　题：Bathymodioline Chemosynthesis Cold seep Deep-sea adaptation Symbiosis 

摘      要：Endosymbiosis with Gammaproteobacteria is fundamental for the success of bathymodioline mussels in deep-sea chemosynthesis-based ecosystems. However, the recent discovery of Campylobacteria on the gill surfaces of these mussels suggests that these host-bacterial relationships may be more complex than previously thought. Using the cold-seep mussel(Gigantidas haimaensis) as a model, we explored this hostbacterial system by assembling the host transcriptome and genomes of its epibiotic Campylobacteria and endosymbiotic Gammaproteobacteria and quantifying their gene and protein expression levels. We found that the epibiont applies a sulfur oxidizing(SOX)multienzyme complex with the acquisition of sox B from Gammaproteobacteria for energy production and switched from a reductive tricarboxylic acid (rTCA) cycle to a Calvin-Benson-Bassham(CBB)cycle for carbon assimilation. The host provides metabolic intermediates, inorganic carbon, and thiosulfate to satisfy the materials and energy requirements of the epibiont, but whether the epibiont benefits the host is unclear. The endosymbiont adopts methane oxidation and the ribulose monophosphate pathway(Ru MP) for energy production, providing the major source of energy for itself and the host. The host obtains most of its nutrients, such as lysine, glutamine, valine,isoleucine, leucine, histidine, and folate, from the endosymbiont. In addition, host pattern recognition receptors, including toll-like receptors, peptidoglycan recognition proteins, and C-type lectins, may participate in bacterial infection, maintenance, and population regulation. Overall, this study provides insights into the complex host-bacterial relationships that have enabled mussels and bacteria to thrive in deep-sea chemosynthetic ecosystems.