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Anti-Human Immunodeficiency Virus Type 1(HIV-1 IIIB) activity of lectins from marine invertebrates

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Anti-Human Immunodeficiency Virus Type 1(HIV-1 IIIB) activit...

Comparing the removal of polycyclic aromatic hydrocarbons in soil after different bioremediation approaches in relation to the extracellular enzyme activities

中国海洋生化学术会议

作者： Valentina Molchanova Irina Chikalovets Dong-Yun Ouyang Stanislav Kobelev Natalia Belogortseva pavel luk’yanov Pacif ic Institute of Bioorganic Chemistry Far East Branch of the Russian Academy of Science 690022 Vladivostok Russia Kunming Institute of Zoology Chinese Academy of Sciences Kunming 650223 China.

We investigated five different specificity lectins from marine *** lectins,DTL and DTL—A from ***,SVL-2 from *** from *** were shown inhibition of infection of the C8166 cell line,byhuman immunodeficiency virus—1 IIIB in ***,DTL—A and CGL displayed inhibition ofcell—cell fusion syncytia formation between chronically infected H9 and uninfected C8166 *** activity was shown by DTL—A and *** our studies,the lectins form marineinvertebrates with different specificity were substantiated by an increase in the inhibitoryactivity in the series:DTL>SVL-2>DTL-A>***-1 didn’t display anti—HIV activity.

关键词： DTL DTL-A CGL SVL-1 SVL-2: HIV-1 IIIB chronically infected H9/HIV-1 IIIB cells

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Journal of Environmental Sciences 2019年第2期31卷 249-258页

作者： Zdeněk Ko?ná? Tereza ?ástková Lucie Wiesnerová luká? Praus Ivan Jablonsky Martin Koudela pavel Tlusto? Department of Agroenvironmental Chemistry and Plant Nutrition Faculty of AgrobiologyFood and Natural ResourcesCzech University of Life Sciences Prague Department of Horticulture Faculty of AgrobiologyFood and Natural ResourcesCzech University of Life Sciences Prague

A 120-day experiment was conducted to compare the removal of polycyclic aromatic hydrocarbons(PAHs) from agricultural soil after natural attenuation(NA), phytoremediation(P), mycoremediation(M), and plant-assisted mycoremediation(PAM) approaches in relation to the extracellular enzyme activities in soil. The NA treatment removed the total soil PAH content negligibly. The P treatment using maize(Zea mays) enhanced only the removal of low and medium molecular PAHs. The Pleurotus ostreatus cultivated on 30–50 mm wood chip substrate used in M treatment was the most successful in the removal of majority PAHs. Therefore,significantly(p < 0.05) highest total PAH removal by 541.4 μg/kg dw(dry weight)(36%) from all tested M treatments was observed. When using the same fungal substrate together with maize in PAM treatment, the total PAH removal was not statistically different from the previous M treatment. However, the maize-assisted mycoremediation treatment significantly boosted fungal biomass, microbial and manganese peroxidase activity in soil which strongly correlated with the removal of total PAHs. The higher PAH removal in that PAM treatment could be reflected in the following post-harvest time. Our suggested M and PAM approaches could be promising in situ bioremediation strategies for PAH-contaminated soils.

关键词： PAHs Biodegradation White-rot fungi Ligninolytic enzymes Microbial activity

Effects of Organic Matter-Rich Amendments on Selenium Mobility in Soils

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Pedosphere 2019年第6期29卷 740-751页

作者： Petra SMAZíKOVá luká? PRAUS Jirina SZáKOVá Jana TREMLOVá Ale? HANC pavel TLUSTOS Department of Agro-Environmental Chemistry and Plant Nutrition Faculty of Agrobiology Food and Natural Resources Czech University of Life Sciences Prague

Soil organic matter (SOM) plays an important role in the Se dynamics in soil. The potential effects of vermicompost and digestate as important sources of SOM on selenium (Se) mobility were assessed in this study. Three soils differing in their physicochemical parameters, fluvisol, chernozem, and luvisol, were chosen, and three types of vermicomposts based on various bio-waste materials as digestate (vermicompost 1), kitchen waste with woodchips (vermicompost 2), and garden bio-waste (vermicompost 3) were used due to their high organic matter content. Additionally, digestate samples alone were applied. To evaluate the potential effect of vermicompost application on sorption characteristics of soils, batch sorption experiments were performed. The results showed a predominant effect on Se species in the soils, where selenite sorbed more intensively compared to selenate, regardless of the soil and ameliorative material applied. In the control, the soil sorption ability of selenite tended to decrease in the order: fluvisol > luvisol > chernozem. However, these differences were not significant. Moreover, the effects of the ameliorative materials depended on both soil and amendment used. In fluvisol, all the amendment applications resulted in a decrease in distribution coefficient ( K d values) of Se, whereas in chernozem, this effect was observed only for the digestate-based vermicompost 1. Increasing K d levels were reported in luvisol treated with digestate;the application of garden bio-waste-based vermicompost 3 tended to decrease the K d values. Further studies are required on long-term effects of these amendments on Se mobility in soils and the role of individual organic matter fractions in this context.

关键词： Batch sorption experiments Bio-waste material Selenite Selenate Sorption isotherms Vermicompost

Tensile and fracture properties of an Mg-RE-Zn alloy at elevated temperatures

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Journal of Rare Earths 2014年第6期32卷 564-572页

作者： TROJANOVZuzanka DONI Tibor luk pavel PALEK Peter CHALUPOV Mária TILLOV Eva BASTOVANSKY Ronald Faculty of Mathematics and Physics Charles University Faculty of Mechanical Engineering University of Zilina

Magnesium alloy EZ10 （Mg-RE-Zn） was deformed in tension at temperatures from 20 up to 520℃. A rapid decrease of the yield and tensile strength with temperature was observed at temperatures higher than 300 ℃. On the other hand, ductility of sam-ples rapidly increased in the same temperature range. Light microscopy and scanning electron microscopy was used to reveal the rea-son for these behaviours. Intermetallic particles in grain boundaries are responsible for excellent mechanical properties at lower tem-peratures. Diffusional processes occurring at temperatures higher than 300 ℃ significantly influenced the deformation mechanism as well as the fracture character.

关键词： magnesium alloy tension dislocation slip twinning dynamic recrystallization grain boundary sliding, fracture, cavitation rare

Enhancement of the ‘tractor-beam’ pulling force on an optically bound structure

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Light(Science & Applications) 2017年第1期6卷 136-141页

作者： Jana Damková lukáš Chvátal Jan Ježek Jindřich Oulehla Oto Brzobohatý pavel Zemánek Institute of Scientific Instruments of the CAS v.v.i.Královopolská147Brno 61264Czech Republic

Recently,increasing attention has been devoted to mastering a new technique of optical delivery of micro-objects tractorbeam’1–*** beams have uniform intensity profiles along their propagation direction and can exert a negative force that,in contrast to the familiar pushing force associated with radiation pressure,pulls the scatterer toward the light *** was experimentally observed that under certain circumstances,the pulling force can be significantly enhanced6 if a non-spherical scatterer,for example,a linear chain of optically bound objects10–12,is optically *** we demonstrate that motion of two optically bound objects in a tractor beam strongly depends on theirs mutual distance and spatial *** configuration-dependent optical forces add extra flexibility to our ability to control matter with *** these interactions opens the door to new applications involving the formation,sorting or delivery of colloidal self-organized structures.

关键词： beam scatter mutual

Pulsed Vector Magnetic Potential Field Existence

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World Journal of Condensed Matter Physics 2012年第4期2卷 202-207页

作者： Ivan Rampl lukás Palko pavel Hyrsl Libor Vojtek ENJOY Ltd. BrnoCzech Republic Masaryk University BrnoCzech Republic

Experimental confirmation discussed the effect of the immediate surroundings of a pulse-powered toroidal coil on biological material which was placed in an environment without the influence of electromagnetic force.

关键词： Vector Magnetic Potential Toroidal Coil Cell Viability Cell Metabolism Bacterium Escherichia Coli

Silicon and III-V Solar Cells: From Modus Vivendi to Modus Operandi

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Green and Sustainable Chemistry 2017年第3期7卷 217-233页

作者： Alexander Buzynin Yury Buzynin Vladimir Shengurov Vladimir Voronkov Ansgar Menke Albert luk’yanov Vitaly Panov Nickolay Baidus Prokhorov General Physics Institute Russian Academy of Sciences Moscow Russia Institute for Physics of Microstructures Russian Academy of Sciences Nizhny Novgorod Russia Lobachevski State University Nizhny Novgorod Russia SunEdison Milano Italy Academy of Science and Education Maico-Mannesmann Hannover Germany Lomonosov Moscow State University Moscow Russia

In the present paper, some novel opportunities for the development of high-efficient Si and III-V-based solar cells are considered: energy-saving environment friendly low-temperature technology of forming p-n junctions in Si (1), elaboration of structurally perfect GaAs/Ge/Si epitaxial substrates (2) and application of protective antireflecting coatings based on cubic zirconia (3). As a result: 1) New technique of forming p-n junctions in silicon has been elaborated. The technique provided easy and comparatively cheap process of production of semiconductor devices such as solar cells. The essence of the technique under the study is comprised in formation p-n junctions in silicon by a change of conductivity in the bulk of the sample occurring as a result of redistribution of the impurities, which already exists in the sample before its processing by ions. It differs from the techniques of diffusion and ion doping where change of conductivity and formation of p-n junction in the sample occur as a result of introduction of atoms of the other dopants from the outside;2) The conditions for synthesis of GaAs/Ge/Si epitaxial substrates with a thin (200 nm) Ge buffer layer featured with (1 - 2) × 105 cm-2 density of the threading dislocation in the GaAs layer. Ge buffer was obtained by chemical vapor deposition with a hot wire and GaAs layer of 1 μm thick was grown by the metal organic chemical vapor deposition. Root mean square surface roughness of GaAs layers of the less than 1 nm and good photoluminescence properties along with their high uniformity were obtained;3) The conditions ensuring the synthesis of uniform functional (buffer, insulating and protective) fianite layers on Si and GaAs substrates by means of magnetron and electron-beam sputtering have been determined. Fianite films have been shown to be suitable for the use as an ideal anti-reflecting material with high protective and anticorrosive properties.

关键词： Solar Cells Green Technologies p-n Junctions Ar Ion-Irradiation Inversion of Conductivity Silicon III-V GaAs on Si Ge Buffer YSZ Antireflection Coatings