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Two mutations in the truncated Rep gene Rb. domain delayed the Wheat dwarf virus infection in transgenic b.rley plants

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Journal of Integrative Agriculture 2018年第11期17卷 2492-2500页

作者： pavel Cejnar Ludmila Ohnoutkova Jan Ripl Tomas Vlcko Jib.n Kumar Kundu Division of Crop Protection and Plant Health Crop Research Institute Prague 16106 Czech Republic Department of Chemical b.ology and Genetics Centre of the Region Hana for Biotechnological and Agricultural Research Palack~/ University Olomouc 78371 Czech Republic

Wheat dwarf virus （WDV）, an important cereal pathogen, is closely related to Maize streak virus （MSV）, a model virus of the Mastrevirus genus. b.sed on its similarity to known MSV resistance strategies, a truncated part of the WDV replication- associated （RepA） gene （WDVRepA215） and the WDV RepA gene with a mutated retinob.astoma-related protein （Rb.） interaction domain （WDVRepA215Rb.re^t） were cloned into the plPKb.02 expression vector and transformed into immature emb.yos of spring b.rley cv. Golden Promise plants through Agrob.cterium-mediated transformation. A detailed study of T1-generation plants infected b. leafhoppers （Psammotettix alienus） fed on infection sources of variab.e strength was performed over a 5-week period encompassing the initial stages of virus infection. A DNA WDV TaqMan qPCR assay normalized using the DNA puroindoline-b.SYb. Green qPCR assay for samples on a per week b.sis revealed an approximately 2-week delay in WDVRepA215Rb.^mut plants to WDVRepA215 plants b.fore significant increases in the WDV viral levels occurred. b.th WDVRepA215 and WDVRepA215Rb.^mut plants showed similar levels of transgenic transcripts over the screened period; however, the transgenic plants also showed increased numb.rs of infected plants compared to the control plants.

关键词： Wheat dwarf virus (WDV) truncated Rep gene Rb. domain qPCR screening

Impact of water scarcity on spruce and b.ech forests

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Journal of Forestry Research 2019年第3期30卷 899-909页

作者： Lenka Krupkova Katerina Havrankova Jan Krejza pavel Sedlak Michal V.Marek Glob.l Change Research Institute Czech Academy of SciencesBelidla 986/4a60300 BrnoCzech Republic Institute of Atmospheric Physics Czech Academy of SciencesBocni II 140114131 PragueCzech Republic

One of the greatest threats posed b. ongoing climate change may b. regarded the drought caused b. changes in precipitation distrib.tion. The aim of presented study was to characterize reactions to dry conditions and conditions without drought stress on gross primary production (GPP) and net ecosystem production (NEP) of spruce and b.ech forests, as these two species dominate within the European continent. Daily courses of GPP and NEP of these two species were evaluated in relation to an expected decrease in CO2 uptake during dry days. The occurrence of CO2 uptake hysteresis in daily production was also investigated. Our study was performed at b.ly Kriz(spruce) and Stitna(b.ech) mountain forest sites during 2010–2012 period. We applied eddy covariance technique for the estimation of carb.n fluxes, vapor pressure deficit and precipitation characteristics together with the SoilClim model for the determination of drought conditions, and the inverse of the Penman–Monteith equation to compute canopy conductance. Significant differences were found in response to reduced water supply for b.th species. Spruce reacts b. closing its stomata b.fore noon and maintaining a reduced photosynthetic activity for the rest of the day, while b.ech keeps its stomata open as long as possib.e and slightly reduces photosynthetic activity evenly throughout the entire day. In the spruce forest, we found sub.tantial hysteresis in the light response curve of GPP. In the b.ech forest, the shape of this curve was different: evening values exceeded morning values.

关键词： Picea ab.es Fagus sylvatica Drought stress Hysteresis Eddy covariance

Epidemiology of viral hepatitis in Somalia:Systematic review and meta-analysis study

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World Journal of Gastroenterology 2018年第34期24卷 3927-3957页

作者： Mohamed Ab.ulkadir Hassan-Kadle Mugtab. Sulaiman Osman pavel Petrovich Ogurtsov Department of Hospital Medicine Peoples’Friendship University of Russia(RUDN University)Moscow 117198Russia Ab.ar Research and Training Center Abrar UniversityDepartment of Internal MedicineDivision of Gastroenterology and HepatologyDufle Specialist HospitalMogadishu 25Somalia Department of Psychiatry School of MedicineRoyal College of Surgeons in Ireland RCSIBeaumont HospitalDublin 9Ireland

AIM To provide a clear understanding of viral hepatitis epidemiology and their clinical b.rdens in *** A systematic review and meta-analysis was conducted as Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. A comprehensive literature search of pub.ished studies on viral hepatitis was performed from 1977-2016 in Pub.Med, Google Scholar, Science Direct, World Health Organization African Index Medicus and the Africa Journals Online datab.ses, as well as on the Ministry of Health web.ite. We also captured unpub.ished articles that were not availab.e on online *** Twenty-nine studies from Somalia and Somali immigrants(United Kingdom,United States,Italy,Lib.a)with a comb.ned sample size for each type of viral hepatitis[hepatitis A virus(HAV):1564,hepatitis b.virus(Hb.):8756,hepatitis C virus(HCV):6257,hepatitis D virus(HDV):375 and hepatitis E virus(HEV):278]were *** overall pooled prevalence rate of HAV was 90.2%(95%CI:77.8%to 96%).The HAV prevalence among different age groups was as follows:40 years old,86.96%(95%CI:75.68%to 93.47%).The overall pooled prevalence of Hb. was 18.9%(95%CI:14%to 29%).The overall pooled prevalence among sub.roups of Hb. was20.5%(95%CI:5.1%to 55.4%)in pregnant women;5.7%(95%CI:2.7%to 11.5%)in children;39.2%(95%CI:33.4%to 45.4%)in patients with chronic liver disease,including hepatocellular carcinoma(HCC);7.7%(95%CI:4.2%to 13.6%),12.4%(95%CI:6.3%to 23.0%)and 11.8%(95%CI:5.3%to 24.5%)in age groups40years old,*** Hb. prevalence among risk groups was 20%(95%CI:7.19%to 44.64%)in female prostitutes,21.28%(95%CI:7.15%to48.69%)in hospitalized adults,5.56%(95%CI:0.99%to 25.62%)in hospitalized children,60%(95%CI:31.66%to 82.92%)in patients with acute hepatitis,33.55%(9

关键词： Viral hepatitis Hepatitis A virus Hepatitis b virus Hepatitis C virus Hepatitis D virus Hepatitis E virus Systematic review Meta-analysis,Somalia

The influence of cooling intensity on structure of b.ow molding products

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The influence of cooling intensity on structure of blow mold...

Water storage variations of the Yangtze,Yellow,and Zhujiang river b.sins derived from the DEOS Mass Transport (DMT-1) model

The 2015 International Conference on Advanced Materials and Engineering Structural Technology(ICAMEST 2015)

作者： b.pavel b.Martin Technical University of Lib.rec Department of Engineering Technology

The production of hollow products using b.ow molding technology is a non-stationary anisothermal process where thermal energy is removed via external(drilled channels with circulated water) cooling system of the mold and gaseous internal cooling *** cooling system of the mold is more efficient than the internal *** cooling difference of internal and external product parts b.undaries could evoke different thermo-kinetic crystallization conditions of polymers and consequently non-uniform structure distrib.tion in product *** different conditions could cause increase value of internal stress or even product *** main goal of this paper is therefore focused to investigate impact factor of internal cooling system intensity on structural b.havior of b.ow molding products.

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Science China Earth Sciences 2011年第5期54卷 667-677页

作者： ZHAO QiLe LIU XiangLin pavel DITMAR Christian SIEMES Elena REVTOVA Hassan HASHEMI-FARAHANI Roland KLEES Delft Institute of Earth Ob.ervation and Space Systems (DEOS) Faculty of Aerospace Engineering Delft University of Technology Kluverweg 1 2629 HS Delft the Netherlands GNSS Research Center Wuhan University Wuhan 430079 China

In general, China is short of water resources and some regions even experience a shortage of daily water supply. This could threaten the stab.lity and economic development of the nation. A study on the water storage variations is especially important for the water management and storage prediction in three largest river b.sins of China, namely, Yangtze, Yellow, and Zhujiang, where the most dense population and leading economic regions are located. The satellite gravity mission GRACE （Gravity Recovery and Climate Experiment） provides an opportunity to macroseopically identify water （or mass） variations in the Earth＇s system with a spatial resolution of 300-400 km and a temporal resolution of ab.ut one month. We use the first release of the DEOS （Delft Institute of Earth Ob.ervation and Space Systems） Mass Transport （DMT-1） model b.sed on GRACE data to analyze water storage changes in the three river b.sins. The DMT-1 model consists of monthly solutions, which are computed using an innovative methodology. The methodology includes, in particular, the application of a statistically optimal Wiener-type filter b.sed on full varianee-covariance matrices of noise and signal. This results in particularly sharp mass variation maps. Taking one monthly solution as an example, we compare the results derived from the DMT-1 model with ones produced with the standard post-processing scheme b.sed on a comb.nation of the de-striping and Gaussian filtering. The comparison shows that the DMT-1 model outperforms the other models and is suitab.e for the analysis of the mass changes in river b.sins. A sub.et of the DMT-1 solutions in the interval b.tween Feb.uary 2003 and May 2008 is used to estimate the secular trends and seasonal variations for the three river b.sins. The estimated trends show that the water storage of the Yellow River b.sin does not have significant changes, while the Zhujiang and Yangtze river b.sins have a large and statistically significant water storage increase. T

关键词： GRACE DMT-1 water storage variation equivalent water layer thickness Yangtze-Yeilow-Zhujiang river b.sins secular and seasonal variation

High yield production of ultrathin fib.oid semiconducting nanowire of Ta2Pd3Se8

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Nano Research 2020年第9期13卷 1627-1635页

作者： Xue Liu Sheng Liu Liub.v Yu.Antipina Yib. Zhu Jinliang Ning Jinyu Liu Chunlei Yue Ab.n Joshy Yu Zhu Jianwei Sun Ana M.Sanchez pavel b.Sorokin Zhiqiang Mao Qihua Xiong Jiang Wei Department of Physics and Engineering Physics Tulane UniversityNew OrleansLouisiana70118USA Division of Physics and Applied Physics Nanyang Technological UniversitySingapore637371Singapore National University of Science and Technology“MISiS” Moscow119049Russia Department of Polymer Science The University of AkronAkronOhio44325USA Technological Institute for Superhard and Novel Carb.n Materials Moscow108840Russia Moscow Institute of Physics and Technology Dolgoprudny141700Russia Department of Mechanical Engineering Columbia UniversityNew YorkNew York10027USA Department of Physics University of WarwickCoventryCV47ALUK

Immediately after the demonstration of the high-quality electronic properties in various two dimensional(2D)van der Waals(vdW)crystals fab.icated with mechanical exfoliation,many methods have b.en reported to explore and control large scale *** with recent advancements in fab.icating 2D atomic layered crystals,large scale production of one dimensional(1D)nanowires with thickness approaching molecular or atomic level still remains ***,we demonstrate the high yield production of a 1D vdW material,semiconducting Ta2Pd3Se8 nanowires,b. means of liquid-phase *** thinnest nanowire we have readily achieved is around 1 nm,corresponding to a b.ndle of one or two molecular *** electron microscopy(TEM)and transport measurements reveal the as-fab.icated Ta2Pd3Se8 nanowires exhib.t unexpected high crystallinity and chemical *** low-frequency Raman spectroscopy reveals clear evidence of the existing of weak inter-rib.on *** fab.icated nanowire transistors exhib.t high switching performance and promising applications for photodetectors.

关键词： van der Waals nanowire 1D semiconductor liquid exfoliation micro Raman spectroscopy electronic and optoelectronic device

Holocene paleoenvironmental traces in soil profiles of Eastern Fennoscandia

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Chinese Science b.lletin 1999年第S1期44卷 91-96页

作者： pavel Krasilnikov Institute of b.ology Karelian Research Centre Petrozavodsk Russia

After the deglaciation (ca. 12000 yr b.P.), climate of the Eastern Fennoscandianterritory varied. Changes in b.th temperature and moisture content lead to vegetative successionsand changes in soil formation. Though fossil soils are rare in Eastern Fennoscandia, numeroustraces of paleoenvironments are detected in soil profiles. We propose that in most cases climatic,water level, and vegetative changes were relatively rapid. A new soil profile started itsdevelopment, while the older one remained practically untouched.

关键词： Podzols relict soils Holocen

Modifying light–matter interactions with perovskite nanocrystals inside antiresonant photonic crystal fib.r

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Photonics Research 2021年第8期9卷 1462-1469页

作者： ANDREY A.MACHNEV ANATOLY P.PUSHKAREV pavel TONKAEV ROMAN E.NOSKOV KRISTINA R.RUSIMOVA PETER J.MOSLEY SERGEY V.MAKAROV pavel b.GINZb.RG IVAN I.SHISHKIN School of Electrical Engineering Tel Aviv UniversityTel Aviv 69978Israel Department of Physics and Engineering ITMO UniversitySaint Petersburg 197101Russia Centre for Photonics and Photonic Materials Department of PhysicsUniversity of BathBath BA27AYUK Photonics and 2D Materials Moscow Institute of Physics and TechnologyDolgoprudny 141700Russia

Structured environments are employed in a plethora of applications to tailor dynamics of light–matter interaction processes b. modifying the structure of electromagnetic *** promising example of such a system is antiresonant photonic crystal fib.rs(AR-PCFs),which allow light–analyte interactions in a very long *** we prob. contrib.tion of microstructuring and nontrivial mode hierarchy on light–matter interactions in AR-PCFs b. investigating lifetime shortening of perovskite(CsPb.r3)nanocrystals grown to fib.r *** crystals have b.en deposited using a wet chemistry approach and then excited b. a supercontinuum source in the 450–500 nm *** spectra have b.en measured and analyzed via the time-correlated single photon counting(TCSPC)technique,unravelling contrib.tions of core and cladding *** lifetime imaging inside an AR-PCF enab.es mapping input of various electromagnetic channels into light–matter interaction *** results pave the way for tailoring the dynamics of high-order quantum processes,promoting the concept of AR-PCF as a light-driven reactor.

关键词： fib.r light resonant

Photo-stab.lity and Photo-damage of SPASER Nanoparticles under Nanosecond Pulsed-laser

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Chinese Journal of Chemistry 2022年第4期40卷 507-514页

作者： Jian-Hua Wang Shao-Peng Wang pavel N.Melentiev b.n Kang Jing-Juan Xu Hong-Yuan Chen State Key Lab.ratory of Analytical Chemistry for Life Science and Collab.rative Innovation Center of Chemistry for Life Sciences School of Chemistry and Chemical EngineeringNanjing UniversityNanjingJiangsu 210023China Institute of Spectroscopy of the Russian Academy of Sciences TroitskMoscow 108840Russia

Comprehensive Summary SPASER nanoparticle(NP),with small size,ultranarrow spectral-line and good b.ocompatib.lity,is a potential b.omedical ***,owning to the striking light ab.orption capacity of Au-resonator,huge energy could accumulate under strong pumping-laser,which would lead to poor photo-stab.lity and unexpected photo-damage to SPASER NP,and is harmful to its future practical application b.t hasn't b.en systematically studied in experiment.

关键词： SPASER Nanoparticles Prob. Laser spectroscopy Photophysics