Two mutations in the truncated Rep gene RBR domain delayed the Wheat dwarf virus infection in transgenic barley plants

作     者：Pavel Cejnar Ludmila Ohnoutkova Jan Ripl Tomas Vlcko Jiban Kumar Kundu 

作者机构：Division of Crop Protection and Plant Health Crop Research Institute Prague 16106 Czech Republic Department of Chemical Biology and Genetics Centre of the Region Hana for Biotechnological and Agricultural Research Palack~/ University Olomouc 78371 Czech Republic 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2018年第17卷第11期

页      面：2492-2500页

核心收录：

学科分类：09[农学] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 0710[理学-生物学] 0832[工学-食品科学与工程（可授工学、农学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 0904[农学-植物保护] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 0713[理学-生态学] 

基　　金：the Czech Ministry of Education, Youth and Sports funding programme LH12161 the Czech Ministry of Agriculture funding programme MZE RO0417 

主　　题：Wheat dwarf virus (WDV) truncated Rep gene RBR domain qPCR screening 

摘      要：Wheat dwarf virus （WDV）, an important cereal pathogen, is closely related to Maize streak virus （MSV）, a model virus of the Mastrevirus genus. Based on its similarity to known MSV resistance strategies, a truncated part of the WDV replication- associated （RepA） gene （WDVRepA215） and the WDV RepA gene with a mutated retinoblastoma-related protein （RBR） interaction domain （WDVRepA215RBRre^t） were cloned into the plPKb002 expression vector and transformed into immature embryos of spring barley cv. Golden Promise plants through Agrobacterium-mediated transformation. A detailed study of T1-generation plants infected by leafhoppers （Psammotettix alienus） fed on infection sources of variable strength was performed over a 5-week period encompassing the initial stages of virus infection. A DNA WDV TaqMan qPCR assay normalized using the DNA puroindoline-b SYBR Green qPCR assay for samples on a per week basis revealed an approximately 2-week delay in WDVRepA215RBR^mut plants to WDVRepA215 plants before significant increases in the WDV viral levels occurred. Both WDVRepA215 and WDVRepA215RBR^mut plants showed similar levels of transgenic transcripts over the screened period; however, the transgenic plants also showed increased numbers of infected plants compared to the control plants.