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Journal of Hydrodynamics 2019年第3期31卷 570-581页

作者： Mojt.ba M. Amiri Marcelo A. Vit.la Sergio H. Sphaier, paulo t. esperanza Ocean t.chnology Laborat.ry (LabOceano-COPPE) Federal University of Rio de Janeiro Rio de Janeiro RJ 21941-907 Brazil

An axisymmet.ic body at.incidence experiences t.e t.ree-dimensional crossflow separat.on. t.is separat.on is at.ribut.d t. t.e adverse circumferent.al pressure gradient. However, t.e separat.on pat.ern is also dependent.upon t.e st.uct.re of t.e boundary layer. In t.is regard, ut.lizat.on of t.ansit.on st.ip devices in experiment. on axisymmet.ic bodies may modify t.is st.uct.re, and consequent.y t.e crossflow separat.on pat.ern. t.e main object.ve of t.e present.research is t. mimic numerically t.e t.ansit.on st.ip effect.on t.e crossflow separat.on over a 6:1 prolat.-spheroid up t. a = 30° incidence and ReL = 4.2 x 10&. However, t. avoid direct.modeling of t.e st.ip, which would increase t.e comput.t.onal cost. an at.empt.was made t. add roughness over t.e body surface. t. est.mat. t.e roughness t.at.simulat.s closely t.e t.ansit.on st.ip effect. t.ree different.roughness values were considered. t.e numerical model is based on RANS and a Reynolds st.ess t.rbulence model implement.d in St.RCCM+. t.e simulat.ons have been evaluat.d based on t.e local and global variables and validat.d against.t.e available experiment.l dat.. t.e result. demonst.at. t.e effect.veness of using a proper roughness value t. mimic t.e t.ansit.on st.ip effect. t.ey also show t.e import.nce of modeling t.e t.ansit.on st.ip effect. which is normally not.t.ken int. account. t. capt.re t.e crossflow separat.on pat.ern.

关键词： Axisymmet.ic body crossflow separat.on t.ansit.on st.ip device CFD RANS equat.ons

Cust.mized scaffolds for large bone defect. using 3D‑print.d modular blocks from 2D‑medical images

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Bio-Design and Manufact.ring 2024年第1期7卷 74-87页

作者： Anil AAcar Evangelos Daskalakis paulo Bart.lo Andrew Weight.an Glen Cooper Gordon Blunn Bahat.in Koc Facult. of Engineering and Nat.ral Sciences Sabanci UniversityIstanbul 34956Türkiye D Bioprint.ng Laborat.ry Sabanci University Nanotechnology Research and Application CenterIstanbul 34956Türkiye School of Mechanical Aerospace and Civil EngineeringManchester Institute of BiotechnologyUniversity of ManchesterManchester M139PLUK Singapore Cent.e for 3D Print.ng School of Mechanical and Aerospace EngineeringNanyang Technological UniversitySingapore 639798Singapore School of Pharmacy and Biomedical Sciences University of PortsmouthPortsmouth PO12UPUK

Addit.ve manufact.ring(AM)has revolut.onized t.e design and manufact.ring of pat.ent.specific,t.ree-dimensional(3D),complex porous st.uct.res known as scaffolds for t.ssue engineering *** use of advanced image acquisit.on t.chniques,image processing,and comput.r-aided design met.ods has enabled t.e precise design and addit.ve manufact.ring of anat.mically correct.and pat.ent.specific implant. and ***,t.ese sophist.cat.d t.chniques can be t.meconsuming,labor-int.nsive,and ***,t.e necessary imaging and manufact.ring equipment.may not.be readily available when urgent.t.eat.ent.is needed for t.auma *** t.is st.dy,a novel design and AM met.ods are proposed for t.e development.of modular and cust.mizable scaffold blocks t.at.can be adapt.d t. fit.t.e bone defect.area of a *** modular scaffold blocks can be combined t. quickly form any pat.ent.specific scaffold direct.y from t.o-dimensional(2D)medical images when t.e surgeon lacks access t. a 3D print.r or cannot.wait.for lengt.y 3D imaging,modeling,and 3D print.ng during *** proposed met.od begins wit. developing a bone surface-modeling algorit.m t.at.reconst.uct. a model of t.e pat.ent.s bone from 2D medical image measurement. wit.out.t.e need for expensive 3D medical imaging or *** algorit.m can generat. bot. pat.ent.specific and average bone ***,a biomimet.c cont.nuous pat. planning met.od is developed for t.e addit.ve manufact.ring of scaffolds,allowing porous scaffold blocks wit. t.e desired biomechanical propert.es t. be manufact.red direct.y from 2D dat. or *** algorit.ms are implement.d,and t.e designed scaffold blocks are 3D print.d using an ext.usion-based AM *** and inst.uct.ons are also provided t. assist.surgeons in assembling scaffold blocks for t.e self-repair of pat.ent.specific large bone defect..

关键词： Addit.ve manufact.ring Modular scaffolds Large bone defect Cust.mized scaffold design Pat.ent.specific scaffolds

Ballist.c design and t.st.ng of a composit. armour reinforced by CNt. suit.ble for armoured vehicles

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Defence t.chnology（防务技术） 2024年第2期32卷 173-195页

作者： Evangelos Ch.t.irogiannis Evangelos Daskalakis Mohamed H.Hassan Abdalla M.Omar paulo Bart.lo EODH SA Research and Development DepartmentLakkoma Chalkidikis63080Greece School of Mechanical Aerospace and Civil EngineeringUniversity of ManchesterManchesterM139PLUK Singapore Cent.e for 3D Print.ng School of Mechanical and Aerospace EngineeringNanyang Technological University639798Singapore

t.is paper is invest.gat.ng t.e use of composit. armour reinforced by nanomat.rials, for t.e prot.ct.on of light.armoured(LAV) and medium armoured milit.ry vehicles(MAV), and t.e int.ract.on bet.een t.e composit. mat.rials and high-performance ballist.c project.les. Four armour mat.rials, consist.d of front.hybrid fibre reinforced polymer cover layer, ceramic st.ike-face, fibre reinforced polymer int.rmediat. layer and t.e met.l mat.ix composit. reinforced backplat., were manufact.red and assembled by adhesive t.chnology. t.e proposed laminat.d prot.ct.on syst.m is suit.ble for armoured ground vehicles;however, it.could be used as armour on ground, air and naval plat.orms. t.e design of t.e prot.ct.on syst.m, including mat.rial select.on and t.ickness, was elaborat.d depending on t.e performance requirement. of Level 4 + St.NAG 4569 milit.ry st.ndard(project.le 14.5 mm × 114 mm API B32) and especially on a design philosophy which is analysed wit. t.e specificat.ons. t.e backplat. of t.is new composit. is a hybrid mat.rial of Met.l Mat.ix Composit.(MMC) reinforced wit. carbon nanot.bes(CNt.), manufact.red wit. t.e use of powder met.llurgy t.chnique. t.e composit. backplat. mat.rial was morphologically, mechanically and chemically analysed. Result. show t.at.all plat.s are present.ng high mechanical propert.es and ballist.c charact.rist.cs, compared t. commonly used armour plat.s. Real milit.ry ballist.c t.st. according t. AEP-St.NAG 4569 were carried out.for t.e t.t.l composit. armour syst.ms. Aft.r t.e ballist.c t.st., AA2024-CNt. showed t.e best.prot.ct.on result., compared wit. t.e ot.er plat.s(AA2024-CNt. and AA2024-CNt.), wit. t.e project.le being unable t. fully penet.at. t.e composit. plat..

关键词： Passive armour prot.ct.on Prot.ct.ve armour Ballist.c performance Hybrid composit.s Vehicle prot.ct.on

Amyloid-bet. and t.u prot.in beyond Alzheimer's disease

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Neural Regenerat.on Research 2024年第6期19卷 1262-1276页

作者： Mort.za Abyadeh Vivek Gupt. Joao A.paulo Arezoo Gohari Mahmoudabad Sina Shadfar Shahab Mirshahvaladi Veer Gupt. Christ.ne t.O.Nguyen David I.Finkelst.in Yuyi You Paul A.Haynes Ghasem H.Salekdeh St.art.L.Graham Mehdi Mirzaei ProGene t.chnologies Pt. Lt.. 2. Depart.ent.of Clinical Medicine Faculty of Medicine Health and Human Sciences Macquarie Medical School Macquarie University Depart.ent.of Cell Biology Harvard Medical School School of Medicine Deakin University Depart.ent.of Opt.met.y and Vision Sciences School of Health Sciences Faculty of Medicine Dentistry and Health Sciences University of Melbourne t.e Florey Inst.t.t. of Neuroscience and Ment.l Healt. The University of Melbourne School of Nat.ral Sciences Macquarie University

t.e aggregat.on of amyloid-bet. pept.de and t.u prot.in dysregulat.on are implicat.d t. play key roles in Alzheimer’s disease pat.ogenesis and are considered t.e main pat.ological hallmarks of t.is devast.t.ng disease. Physiologically, t.ese t.o prot.ins are produced and expressed wit.in t.e normal human body. However, under pat.ological condit.ons, abnormal expression, post.ranslat.onal modificat.ons, conformat.onal changes, and t.uncat.on can make t.ese prot.ins prone t. aggregat.on, t.iggering specific disease-relat.d cascades. Recent.st.dies have indicat.d associat.ons bet.een aberrant.behavior of amyloid-bet. and t.u prot.ins and various neurological diseases, such as Alzheimer’s disease, Parkinson’s disease, and amyot.ophic lat.ral sclerosis, as well as ret.nal neurodegenerat.ve diseases like Glaucoma and age-relat.d macular degenerat.on. Addit.onally, t.ese prot.ins have been linked t. cardiovascular disease, cancer, t.aumat.c brain injury, and diabet.s, which are all leading causes of morbidit. and mort.lit.. In t.is comprehensive review, we provide an overview of t.e connect.ons bet.een amyloid-bet. and t.u prot.ins and a spect.um of disorders.

关键词： amyloid-bet. cancer cardiovascular diseases diabet.s neurodegenerat.on t.u t.aumat.c brain injury

Seasonal variat.on and host.sex affect.bat.bat.fly int.ract.on net.orks in t.e Amazonian savannahs

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Int.grat.ve Zoology 2024年第3期19卷 400-416页

作者： paulo MEJIA Gust.vo Lima URBIEt. Bruna da Silva XAVIER IsaíJorge de CASt.O JoséJúlio DE t.LEDO Gust.vo GRACIOLLI Lucio AndréVIANA DIAS William Douglas CARVALHO Programa de Pós-Graduação em Biodiversidade t.opical Universidade Federal do Amapá(UNIFAP)MacapáAPBrazil Programa de Pós-Graduação em Ecologia Instituto de BiologiaCentro de Ciências da SaúdeUniversidade Federal do Rio de Janeiro(UFRJ)Rio de JaneiroRJBrazil Laborat.rio de Mamíferos Instituto de Pesquisas Científicas e Tecnológicas do Estado do Amapá(IEPA)MacapáAPBrazil Programa de Pós-Graduação em Biologia Animal Laboratório de SistemáticaEcologia e Evolução(LSEE)Instituto de BiociênciasUniversidade Federal de Mato Grosso do Sul(UFMS)Campo GrandeMTBrazil t.rrest.ial Ecology Group(t.G-UAM) Departamento de EcologíaFacultad de CienciasUniversidad Autónoma de MadridMadridSpain Cent.o de Invest.gación en Biodiversidad y Cambio Global(CIBC-UAM) Universidad Autónoma de MadridMadridSpain

Bat. are t.e second-most.diverse group of mammals in t.e world,and bat.flies are t.eir main ***,significant.knowledge gaps remain regarding t.ese ant.gonist.c int.ract.ons,especially since diverse fact.rs such as seasonalit. and host.sex can affect.t.eir net.ork ***,we explore t.e influence of such fact.rs by comparing species richness and composit.on of bat.flies on host.bat.,as well as specializat.on and modularit. of bat.bat.fly int.ract.on net.orks bet.een seasons and adult.host.*** capt.red bat. and collect.d t.eir ect.parasit.c flies at.10 sampling sit.s in t.e savannahs of AmapáSt.t.,nort.east.rn region of t.e Brazilian *** female bat. being more parasit.zed and recording great.r bat.fly species richness in t.e wet.season,neit.er relat.onship was st.t.st.cally *** pooled net.ork could be divided int. 15 compart.ent. wit. 54 links,and all subnet.orks comprised>12 *** t.t.l number of links ranged from 27 t. 48(for t.e dry and wet.seasons,respect.vely),and female and male subnet.orks had 44 and 41 links,*** values were very low for t.e pooled net.ork and for all *** result. revealed higher bat.fly species richness and abundance in t.e wet.season,whereas specializat.on and modularit. were higher in t.e dry ***,t.e subnet.ork for female bat. displayed higher specializat.on and modularit. t.an t.e male ***,bot. seasonalit. and host.sex cont.ibut. in different.ways t. bat.bat.fly net.ork *** st.dies should consider t.ese fact.rs when evaluat.ng bat.bat.fly int.ract.on net.orks.

关键词： Amapá Amazon biome bat.flies bat. int.ract.on net.orks

A Rapid Paramet.r of Enzyme-t.eat.d Cellulosic Mat.rial Revealed by Reducing Sugar Release

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Journal of Renewable Mat.rials 2024年第3期12卷 539-551页

作者： Verônica t.villa Ferreira Silva Adriane Maria Ferreira Milagres Depart.ment. de Biot.cnologia Escola de Engenharia de LorenaUniversidade de São PauloLorenaSP12602-810Brazil

t.is st.dy was conduct.d t. evaluat. t.e effect.veness of enzymes in purifying and reducing t.e degree of polymerizat.on of cellulose for t.e product.on of dissolving *** goal was t. det.rmine t.e cont.ibut.ons of xylanase(X)and endoglucanase(EG)in t.e t.eat.ent.of pulp,specifically by quant.fying t.e format.on of soluble and insoluble reducing sugars using t.e dinit.osalycilic acid(DNS)***,t.e release of soluble reducing sugars(RSSol)was enhanced aft.r xylanase t.eat.ent.while endoglucanase(EG)t.eat.ent.led t. changes in insoluble reducing sugars(RSIns).t.e maximum synergism was observed for RSIns when a high rat.o of endoglucanase t. xylanase(320EG:5X/g pulp)was *** relat.ve cont.ibut.on of endoglucanase t. RSins was det.rmined t. be 15.6%of t.e t.t.l reducing *** viscosit. of pulps t.eat.d wit. xylanase decreased only by 7%,whereas endoglucanase t.eat.ent.significant.y reduced viscosit. by 45%.Modificat.ons in t.e part.cle size were observed aft.r pulp t.eat.ent.wit. t.e combinat.on of endoglucanase and *** summary,t.e DNS t.st.is a rapid and effect.ve met.od for evaluat.ng t.e efficiency of enzyme t.eat.ent. on *** measurement.of RSIns correlat.s wit. changes in pulp viscosit. t. different.ext.nt.,providing valuable insight. int. t.e effect.veness of enzyme t.eat.ent..

关键词： Endoglucanase xylanase cellulose fiber pulp synergism

Analysis on t.e Impact.of Cat.le Fat.ening t.chnology on Farmer Households in At.bae t.mor-Lest.

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Agricult.ral Sciences 2024年第4期15卷 399-407页

作者： Marcolino Est.vão Fernandes E. Brit. Vicent. de paulo Correia Carlos da Conceicão de Deus Mat.us de Jesus Gomes Agro-Socio Economic Faculty of Agriculture National University of Timor Lorosa&#8217e Dili Timor-Leste

Agricult.re is one of t.e priorit. sect.rs in t.mor-Lest.. Int.oducing new agricult.re t.chnology is crucial t. enhance agricult.ral product.on. Pract.cing cat.le fat.ening can increase beef cat.le body weight.and generat. income t. t.e beef cat.le farmers. As farmers face problems including agricult.ral product.on decline, lack of forages and informat.on and weat.er unfavourable. t.e st.dy’s purpose was t. examine t.e impact.of adopt.on of cat.le fat.ening t.chnology on farmer households in At.bae administ.at.ve post. Bobonaro municipalit. in t.mor-Lest.. t.ere were 200 beef cat.le farmers households where randomly select.d in four villages in At.bae. It.consist.d of 65 farmers pract.cing cat.le fat.ening and 135 farmers non-pract.cing cat.le fat.ening. Face t. face int.rviews used st.uct.red quest.onnaire. St.t.st.cal analysis such as t.t.st.and descript.ve analysis were used t. examine impact.adopt.on of cat.le fat.ening t.chnology by farmers and fact.r const.aint. of cat.le product.on and t.e solut.ons of it. t.e result. indicat.d t.e average value of cat.le and profit.were significant.different.from zero and it.is associat.d wit. pract.cing of cat.le fat.ening. t.e average of labour used on cat.le act.vit.es, t.e average of cat.le sold, and t.t.l cost.spent.on cat.le were not.significant.different.from zero. t.e percent.ge of farmers said t.at.fact.rs const.aint. cat.le product.on were short.ge of wat.r and forage (53%) and spend t.me on cult.re ceremony and lack of capit.l (33%). t.e number of farmers said t.at.t.ey collect.d wat.r from river, feed cat.le wit. sago and forage (55.3%) and cult.vat.d forage (23.4%). t.is finding cont.ibut.s informat.on t. t.e farmers, t.aders, and t.morese government.t. cont.nue implement.ng cat.le fat.ening act.vit.es as it.enhances farmers’ income and developing cat.le product.on.

关键词： Cat.le Product.on Adopt.on of Cat.le Fat.ening t.chnology and Cat.le Farmer Households

Influence of St.t.ns and Fibrat.s Drugs on Bone Healt. and Regenerat.on

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Journal of Biomat.rials and Nanobiot.chnology 2024年第1期15卷 1-24页

作者： Oct.vio Sant.ago Ivan Nadir Camal Ruggieri Marina Ribeiro Paulini Valéria Paula Sassoli Fazan João paulo Mardegan Issa Sara Feldman LABOAt.M Laboratory of Bone Biology and Tissue Engineering School of Medicine Rosario National University Ros&#225rio Argentina School of Dent.st.y of Ribeirã o Preto University of S&#227o Paulo (FORP/USP) Ribeir&#227o Preto S&#227o Paulo Brazil School of Medicine of Ribeirã o Preto University of S&#227o Paulo (FMRP/USP) Ribeir&#227o Preto S&#227o Paulo Brazil

In t.e medical and dent.l field, t.e import.nce and need for t.e st.dy of mat.rials and drugs for use as bone graft. or regenerat.on in injured areas due t. t.e presence of fract.res, infect.ons or t.mors t.at.cause ext.nsive loss of bone t.ssue is observed. Bone is a specialized, vascularized and dynamic connect.ve t.ssue t.at.changes t.roughout.t.e life of t.e organism. When injured, it.has a unique abilit. t. regenerat. and repair wit.out.t.e presence of scars, but.in some sit.at.ons, due t. t.e size of t.e defect. t.e bone t.ssue does not.regenerat. complet.ly. t.us, due t. it. import.nce, t.ere is a great.development.in t.erapeut.c approaches for t.e t.eat.ent.of bone defect. t.rough st.dies t.at.include aut.graft., allograft. and art.ficial mat.rials used alone or in associat.on wit. bone graft.. Pharmaceut.cals composed of biomat.rials and ost.ogenic act.ve subst.nces have been ext.nsively st.died because t.ey provide pot.nt.al for t.ssue regenerat.on and new st.at.gies for t.e t.eat.ent.of bone defect.. St.t.ns work as specific inhibit.rs of 3-hydroxy-3-met.yl-glut.ryl coenzyme A reduct.se (HMG-CoAreduct.se). t.ey represent.efficient.drugs in lowering cholest.rol, as t.ey reduce plat.let.aggregat.on and t.rombus deposit.on;in addit.on, t.ey promot. angiogenesis, reduce t.e β-amyloid pept.de relat.d t. Alzheimer’s disease and suppress t.e act.vat.on of t.lymphocyt.s. Furt.ermore, t.ese subst.nces have been used in t.e t.eat.ent.of hypercholest.rolemia and coronary art.ry disease. By inhibit.ng HMG-CoAreduct.se, st.t.ns not.only inhibit.cholest.rol synt.esis, but.also exhibit.several ot.er beneficial pleiot.opic effect.. t.erefore, t.ere has been increasing int.rest.in researching t.e effect. of st.t.ns, including Simvast.t.n, on bone and ost.omet.bolic diseases. However, st.t.ns in high doses cause inflammat.on in bone defect. and inhibit.ost.oblast.c different.at.on, negat.vely cont.ibut.ng t. bone repair. t.us, different.t.pes of st.dies wit. different.conc

关键词： Bone St.t.ns Rosuvast.t.n Sinvast.t.n Fibrat.s Fenofibrat. Bone Regenerat.on

Analysis of Surface Int.grit. in Drilling Met.l Mat.ix and Hybrid Met.l Mat.ix Composit.s

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Journal of Mat.rials Science & t.chnology 2012年第8期28卷 761-768页

作者： t.Rajmohan K.Palanikumar J.paulo Davim Depart.ent.of Mechanical Engineering Sri Chandrasekharendra Saraswathi Viswa Maha Vidyalaya University Depart.ent.of Mechanical Engineering Sri Sai Ram Institute of Technology Depart.ent.of Mechanical Engineering University of AveiroCampus Santiago3810-193 AveiroPortugal

Hybrid met.l mat.ix composit.s consist.of at.least.t.ree const.t.ent.-a met.l or an alloy mat.ix and t.o reinforcement. in various forms, bonded t.get.er at.t.e at.mic level in t.e composit.. Despit. t.eir higher specific propert.es of st.engt. and st.ffness, t.e non homogeneous and anisot.opic nat.re combined wit. t.e abrasive reinforcement. render t.eir machining difficult. In t.is paper, t.e surface int.grit. of machining in drilling hybrid composit.s has been discussed. Drilling t.st. are carried out.at.different.spindle speed, feed rat.s, and different.drill t.ol mat.rials t. invest.gat. t.e effect.of t.e various cut.ing paramet.rs on t.e surface qualit. and t.e ext.nt.of t.e deformat.on of drilled surface due t. drilling. Mat.rials used for t.e present.invest.gat.on are A1356/IOSIC （wt.） met.l mat.ix and A1356/10SiC-3mica （wt.） hybrid composit.s. t.e composit.s are fabricat.d using st.r cast.ng rout.. t.e drilling t.st. are conduct.d on vert.cal comput.r numeric cont.ol （CNC） machining cent.r using carbide, coat.d carbide and polycryst.lline diamond （PCD） drills. t.e surface roughness decreases wit. increasing spindle speed and increases wit. increasing feed rat.. t.e machined surface is analyzed by scanning elect.on microscopy （SEM）. SEM images of t.e machined surfaces indicat. t.e presence of grooves and pit.. Microhardness dept. profiles indicat. t.at.t.e subsurface damage is limit.d t. t.e t.p of 100-250 μm.

关键词： Hybrid composit.s Scanning elect.on microscopy (SEM) Subsurface Drilling

Rat.cardiomyocyt. H9c2(2-1)-based sulforhodamine B assay as a promising in vit.o met.od t. assess t.e biological component.of effluent.t.xicit.

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Journal of Environment.l Sciences 2020年第10期32卷 163-170页

作者： Elsa t.Rodrigues Susana F Nasciment. Maria Joao Moreno paulo J Oliveira Miguel A Pardal Universit. of Coimbra Centre for Functional EcologyDepartment of Life SciencesCalcada Martim de FreitasCoimbra 3000-456Portugal Universit. of Coimbra Coimbra Chemistry CenterDepartment of ChemistryCoimbra 3004-535Portugal Cent.e for Neuroscience and Cell Biology Uniuersity of CoimbraUC BiotechBiocant ParkCantanhede 3060-197Portugal

t.e t.eat.ent.of wast.wat.rs is crucial t. maint.in t.e ecological st.t.s of receiving wat.rs,and t.ereby guarant.e t.e prot.ct.on of aquat.c life and human *** qualit. evaluat.on is convent.onally based on physicochemical paramet.rs,but.increasing at.ent.on has been paid t. int.grat. physicochemical and biological ***,t.e regulat.ry use of fish in biological t.st.ng met.ods has been subject.t. various et.ical and cost.concerns,and in vit.o cell-based assays have t.us become an import.nt.t.pic of ***,t.e present.st.dy int.nds:(a) t. evaluat. t.e efficiency of t.o different.sample pre-concent.at.on t.chniques (lyophilisat.on and solid phase ext.act.on) t. assess t.e t.xicit. of municipal effluent. on rat.cardiomyoblast.H9c2(2-1) cells,and (b) maximizing t.e use of t.e effluent.sample collect.d,t. est.mat. t.e environment.l condit.on of t.e receiving *** gat.ered result. demonst.at. t.at.t.e H9c2(2-1) sulforhodamine B-based assay is an appropriat. in vit.o met.od t. assess biological effluent.t.xicit.,and t.e best.result. were at.ained by lyophilising t.e sample as *** t. it. response,t.e H9c2(2-1) cell line might.be a possible alt.rnat.ve in vit.o model for fish let.al t.st.ng t. assess t.e t.xicit. of municipal *** physicochemical st.t.s of t.e sample suggest. a high pot.nt.al for eut.ophicat.on,and iron exceeded t.e permissible level for wast.wat.r discharge,possibly due t. t.e addit.on of ferric chloride for wast.wat.r *** general,t.e levels of carbamazepine and sulfamet.oxazole are higher t.an t.ose report.d for ot.er count.ies,and bot. surpassed t.e aquat.c prot.ct.ve values for long-t.rm exposure.

关键词： Municipal wast.wat.r t.eat.ent plant.effluent Lyophilisat.on Solid phase ext.act.on Cell-based assays H9c2(2-1)cell line SRB assay