Rat cardiomyocyte H9c2(2-1)-based sulforhodamine B assay as a promising in vitro method to assess the biological component of effluent toxicity

作     者：Elsa T Rodrigues Susana F Nascimento Maria Joao Moreno Paulo J Oliveira Miguel A Pardal Elsa T Rodrigues;Susana F Nascimento;Maria Jo(a)o Moreno;Paulo J Oliveira;Miguel A Partdal

作者机构：University of CoimbraCentre for Functional EcologyDepartment of Life SciencesCalcada Martim de FreitasCoimbra 3000-456Portugal University of CoimbraCoimbra Chemistry CenterDepartment of ChemistryCoimbra 3004-535Portugal Centre for Neuroscience and Cell BiologyUniuersity of CoimbraUC BiotechBiocant ParkCantanhede 3060-197Portugal 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2020年第32卷第10期

页      面：163-170页

核心收录：

学科分类：083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 082803[工学-农业生物环境与能源工程] 08[工学] 0828[工学-农业工程] 0804[工学-仪器科学与技术] 

基　　金：The Portuguese Foundation for Science and Technology (FCT) supported the present study through a fellowship attributed to Elsa Teresa Rodrigues(SFRH/BPD/116152/2016),which was funded by the Human Potential Operating Program of the European Social Fund,and by the Portuguese budget through the Ministry of Education and Science developed within the Fish Free Project(PTDC/AAG-TEC/4966/2014) supported by the FCT through national funds (3599-PPCDT) the co-funding of the European Regional Development Fund (POCI-01-0145-FEDER-016875) supported within the PT2020 Partnership Agreement and COMPETE 2020 by the centre for Functional Ecology Strategic Project (UID/BIA/04004/2013) the Coimbra Chemistry Center Strategic Project (UID/QUI/00313/2013) 

主　　题：Municipal wastewater treatment plant effluent Lyophilisation Solid phase extraction Cell-based assays H9c2(2-1)cell line SRB assay 

摘      要：The treatment of wastewaters is crucial to maintain the ecological status of receiving waters,and thereby guarantee the protection of aquatic life and human *** quality evaluation is conventionally based on physicochemical parameters,but increasing attention has been paid to integrate physicochemical and biological ***,the regulatory use of fish in biological testing methods has been subject to various ethical and cost concerns,and in vitro cell-based assays have thus become an important topic of ***,the present study intends:(a) to evaluate the efficiency of two different sample pre-concentration techniques (lyophilisation and solid phase extraction) to assess the toxicity of municipal effluents on rat cardiomyoblast H9c2(2-1) cells,and (b) maximizing the use of the effluent sample collected,to estimate the environmental condition of the receiving *** gathered results demonstrate that the H9c2(2-1) sulforhodamine B-based assay is an appropriate in vitro method to assess biological effluent toxicity,and the best results were attained by lyophilising the sample as *** to its response,the H9c2(2-1) cell line might be a possible alternative in vitro model for fish lethal testing to assess the toxicity of municipal *** physicochemical status of the sample suggests a high potential for eutrophication,and iron exceeded the permissible level for wastewater discharge,possibly due to the addition of ferric chloride for wastewater *** general,the levels of carbamazepine and sulfamethoxazole are higher than those reported for other countries,and both surpassed the aquatic protective values for long-term exposure.