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In vitro induced pluripotency from urine-derived cells in porcine

World Journal of Stem Cells 2022年第3期14卷 231-244页

作者： Kaiana Recchia Lucas Simões Machado Ramon Cesar Botigelli Naira Caroline Godoy Pieri Gabriela Barbosa Raquel Vasconcelos Guimarães de Castro Mariana Groke Marques Laís Vicari de Figueiredo Pessôa paulo fantinato neto Flávio Vieira Meirelles Aline Fernanda de Souza Simone Maria Massami Kitamura Martins Fabiana Fernandes Bressan Department of Surgery Faculty of Veterinary Medicine and Animal SciencesUniversity of São PauloPirassununga 13635-000São PauloBrazil Department of Pharmacology and Biotechnology Institute of BioscienceSão Paulo State UniversityBotucatu 18618-689São PauloBrazil Department of Veterinary Medicine Faculty of Animal Sciences and Food EngineeringUniversity of São PauloPirassununga 13635-000São PauloBrazil Embrapa Suínos e Aves Empresa Brasileira de Pesquisa AgropecuáriaConcordia 89715-899Santa CatarinaBrazil Department of Animal Sciences Faculty of Animal Sciences and Food EngineeringUniversity of São PauloPirassununga 13635-000São PauloBrazil

BACKGROUND The generation of induced pluripotent stem cells(iPSC)has been a game-changer in translational and regenerative medicine;however,their large-scale applicability is still hampered by the scarcity of accessible,safe,and reproducible *** porcine model is a large biomedical model that enables translational applications,including gene editing,long term in vivo and offspring analysis;therefore,suitable for both medicine and animal *** To reprogramme in vitro into pluripotency,and herein urine-derived cells(UDCs)were isolated from porcine *** The UDCs were reprogrammed in vitro using human or murine octamer-binding transcription factor 4(OCT4),SRY-box2(SOX2),Kruppel-like factor 4(KLF4),and C-MYC,and cultured with basic fibroblast growth factor(bFGF)*** characterize the putative porcine iPSCs three clonal lineages were submitted to immunocytochemistry for alkaline phosphatase(AP),OCT4,SOX2,NANOG,TRA181 and SSEA 1 *** transcripts related to the pluripotency(OCT4,SOX2 and NANOG)were analyzed via reverse transcription quantitative realtime polymerase chain reaction in different time points during the culture,and all three lineages formed embryoid bodies(EBs)when cultured in suspension without bFGF *** The UDCs were isolated from swine urine samples and when at passage 2 submitted to in vitro *** of putative iPSCs were obtained only from UDCs transduced with the murine factors(mOSKM),but not from human factors(hOSKM).Three clonal lineages were isolated and further cultured for at least 28 passages,all the lineages were positive for AP detection,the OCT4,SOX2,NANOG markers,albeit the immunocytochemical analysis also revealed heterogeneous phenotypic profiles among lineages and passages for NANOG and SSEA1,similar results were observed in the abundance of the endogenous transcripts related to pluripotent *** the clonal lineages when cultured in suspension without

关键词： Induced pluripotent stem cells Noninvasive Pluripotency Reprogramming Urine Porcine

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Percutaneous nephrostomy versus ureteral stent in hydronephrosis secondary to obstructive urolithiasis:A systematic review and meta-analysis

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Asian Journal of Urology 2024年第2期11卷 261-270页

作者： Andreia Cardoso Aparício Coutinho Gonçalo neto Sara Anacleto Catarina Laranjo Tinoco Nuno Morais Mário Cerqueira-Alves Estevão Lima paulo Mota Department of Urology Hospital de BragaBragaPortugal School of Medicine University of MinhoBragaPortugal Association(2CA-Braga).School of Medicine University of MinhoBragaPortugal

Objective: To assess if there is a preferable intervention between retrograde ureteral stent (RUS) and percutaneous nephrostomy (PCN) tube, in cases of upper urinary tract stone obstruction with complications requiring urgent drainage, by evaluating outcomes regarding urinary symptoms, quality of life (QoL), spontaneous stone passage, and length of hospital stays, since there is no literature stating the superiority of one modality over the ***: We searched MEDLINE and other sources for relevant articles in June 2019 without any date restrictions or filters applied. The selection was done first by the title and abstract screening and then by full-text assessment for eligibility. Only randomized controlled trials or cohort studies in patients with hydronephrosis secondary to obstructive urolithiasis that presented comparative data between PCN and RUS placement concerning at least one of the defined outcome measures were included. Lastly, MEDLINE database and PubMed platform were screened again using the same terms, from June 2019 until November ***: Of 556 initial articles, seven were included in this review. Most works were considered of moderate-to-high quality. Three studies regarding QoL showed a tendency against stenting, even though only one demonstrated statistically significant negative impact on overall health state. Two works reported significantly more post-intervention urinary symptoms in stenting patients. One article found that PCN is a significant predictor of spontaneous stone passage, when adjusted for stone size and location. Findings on length of hospital stays were not consistent among ***: PCN appears to be the intervention better tolerated, with less impact on the patient’s perceived QoL and less post-operative urinary symptoms, in comparison with RUS. Nevertheless, further studies with larger samples and a randomized controlled design are suggested.

关键词： Ureteral stent Percutaneous nephrostomy Obstructive urolithiasis Urinary symptoms Quality of life

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Influence of biological maturation on cardiac autonomic recovery in female volleyball players during&after repeated sprints training:An experimental trial

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Sports Medicine and Health Science 2024年第3期6卷 279-286页

作者： paulo Francisco de Almeida-neto Fernanda Cristina Silva de Oliveira Jose Marcondes de Oliveira-Júnior Júlio Cesar Medeiros Alves Matheus de Lima Rocha Iago Medeiros da Silva Roberto Felipe C^amara Rocha paulo Moreira Silva Dantas Breno Guilherme de Araújo Tin^oco Cabral Health Sciences Center Federal University of Rio Grande do NorteCCS-UFRNNatal59012-570RNBrazil Department of Physical Education Federal University of Rio Grande do NorteDEF-UFRNNatal59078-970RNBrazil

Previously,it was suggested that biological maturation(BM)could be linked to cardiac autonomic recovery(CAR)in the pediatric ***,this influence hasn’t been confirmed *** aim was to investigate the impact of BM on CAR in female volleyball *** study with a sample of 38 volleyball players,comprising 20 girls(age:[11.62.1]years)and 18 women(age:[24.55.5]years),we analyzed BM,comparing maturing subjects(girls)with mature subjects(women).Additionally,we assessed peak height velocity(PHV)in *** conducted a training session involving repeated sprints(3 rounds of 6 sprints interspersed by 5 min[min]of passive rest).Using short-range radio telemetry,we analyzed CAR during(at the end of the 1st and 2nd rounds)and after(following the 3rd round)the training session of repeated sprints by applying the 60-s to 300-s heart rate recovery index(HRR-Index).Girls exhibited superior CAR compared to women(round 2:60-s,120-s,240-s,and 300-s,p0.4,p0.4,p<0.05).We have concluded that biological maturation has a significant impact on cardiac autonomic recovery.

关键词： Exercise Sport Volleyball Heart rate Pediatric

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Combined Oxidative Phosphorylation Deficiency-20-Exome as a Diagnostic Implement

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Journal of Biosciences and Medicines 2024年第6期12卷 7-12页

作者： paulo Roberto Matos-neto Lucas Antonissen Lima Verde Airton Ferreira da Ponte-Filho Luís Eduardo Oliveira Matos Amandha Espavier Trés paulo Roberto Lacerda Leal Gerardo Cristino-Filho Regina Coeli de Carvalho Porto Carneiro Faculty of Medicine Federal University of Cear Sobral Cear Brazil Department of Neurosurgery Federal University of Cear Sobral Cear Brazil Department of Neuropediatrics Federal University of Cear Sobral Cear Brazil

Mitochondrial disorders are phenotypically varied, with serious clinical repercussions. Among them, there is the deficiency of combined oxidative phosphorylation of type 20, which occurs due to a defect in the VARS2 gene. This article presents a case of a 2-year-old female with progressive myoclonic epilepsy and psychomotor regression, with refractoriness to multiple anticonvulsants. The diagnosis was only made after the examination was carried out. Therefore, this article highlights the aspects of this rare disease and the importance of the exome for the diagnosis of rare conditions.

关键词： Oxidative Phosphorylation Epilepsy Exome Mitochondrial Defect VARS2

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Changes in calcium accumulation and utilization efficiency and their impact on recycling,immobilization,and export across the oil palm cycle

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Oil Crop Science 2024年第3期9卷 143-150页

作者： Ismael de Jesus Matos Viégas Luma Castro de Souza Eric Victor de Oliveira Ferreira Milton Garcia Costa Glauco André dos Santos Nogueira Vitor Resende do Nascimento Candido Ferreira de Oliveira neto Capanema Campus Federal Rural University of the Amazon(UFRA)Av.Barao de CapanemaCaixa D'agua5514-6434ParaBrazil Center of Agricultural and Environmental Sciences Federal University of Maranhao(UFMA)ChapadinhaBR-222km 04SNBoa Vista65500000MaranhaoBrazilc Capitao Poço Campus Federal Rural University of the Amazon(UFRA)Tv.Pau AmareloS/n-Vila Nova68650-000Capitao PoçoParaBrazil Department of Soil Science Sao Paulo State University(Unesp)School of Agricultural and Veterinarian SciencesJaboticabalVia de Acesso Prof.Paulo Donato CastelaneCastellane-S/N-Vila Industrial14884-900Sao PauloBrazil Institute of Agricultural Sciences(ICA) Federal Rural University of the Amazon(UFRA)Estr.Principal da Ufra2150Curio-Utinga66077-580BelemParaBrazil

Effective calcium(Ca)management is crucial for optimizing oil palm cultivation and enhancing crop *** study aimed to gain insights into the dynamics of Ca concentration,accumulation,exportation,immobilization,and recycling in various oil palm organs relative to plant *** experiment was conducted at the Agropalma enterprise site in the northeastern region of Para State,Brazil,evaluating seven plant age treatments:2,3,4,5,6,7,and 8 years *** a completely randomized design with four *** results demonstrated an age-related increase in Ca concentration in petioles,rachis,arrows,male inflorescences,peduncles,and ***,Ca accumulation exhibited an upward trend in all organs with progressing plant ***,the study revealed an enhanced Ca use efficiency across all plant organs in correlation with the age of oil palm *** findings underscore the dynamic nutritional demands of oil palm,influencing Ca immobilization,cycling,and export throughout its developmental stages.

关键词： Elaeis guineensis Jacq Ca cycling Ca export Ca immobilization Ca use efficiency Plant nutrition Amazon

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Effect of drug therapies on self-reported chemosensory outcomes after COVID-19

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World Journal of Otorhinolaryngology-Head and Neck Surgery 2024年第2期10卷 88-96页

作者： Marco A.Fornazieri Bruno M.Cunha Samuel P.Nicácio Lucas K.Anzolin JoséL.B.da Silva Aristides Fernandes neto Deusdedit Brandão neto Richard L.Voegels Fábio D.R.Pinna Department of Surgery Londrina State UniversityLondrinaBrazil Department of Medicine Pontifical Catholic University of ParanáLondrinaBrazil Department of Otorhinolaryngology University of São PauloSao PauloBrazil Department of Otorhinolaryngology:Head and Neck Surgery Perelman School of MedicineSmell and Taste CenterUniversity of PennsylvaniaPhiladelphiaPennsylvaniaUSA

Objective:The aim of this study was to assess the relative efficacy of medications used following severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)infection on self-reported alterations in taste and/or smell ***:Seven hundred and fourteen persons with self-reported postcoronavirus disease 2019(post-COVID-19)chemosensory disorders were personally interviewed regarding specific medications they were administered following the acute phase of the *** dependent measure—self-reported total recovery of chemosensory symptoms—was subjected to stepwise logistic *** predictors included demographic and clinical variables,in addition to specific medications used to mitigate disease symptoms(i.e.,systemic corticosteroids,oseltamivir,vitamin C,ibuprofen,hydroxychloroquine,azithromycin,ivermectin,nitazoxanide,anticoagulants,and zinc).Results:The median time between COVID-19 symptom onset and the interviews was 81 days(interquartile range:60-104).Of the 714 subjects,249(34.9%)reported total recovery of their chemosensory function;437(61.2%)had at least one treatment since the beginning of the *** and those with more comorbidities had undergone more *** recovery rates of the treated and nontreated groups did not differ ***,respondents who had used nitazoxanide tended to have a higher rate of self-reported taste or smell *** who took oral zinc were less likely to ***:No medication employed during the first months after SARS-CoV-2 infection had a clear positive effect on returning self-reported smell or taste function to normal,although nitrazoxide trended in a positive *** zinc had a negative effect on the reported recovery of these senses.

关键词： chemosensory disorder coronavirus infections COVID-19 drug therapy SARS-CoV-2 smell loss taste loss

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Systematic Review on Ground-Based Cloud Tracking Methods for Photovoltaics Nowcasting

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American Journal of Climate Change 2024年第3期13卷 452-476页

作者： Juliana Marian Arrais Allan Cerentini Bruno Juncklaus Martins Thiago Zimmermann Loureiro Chaves Sylvio Luiz Mantelli neto Aldo von Wangenheim PPGCC-INE UFSC Federal University of Santa Catarina Florianpolis Brazil INPE Brazilian National Institute for Space Research So Paulo Brazil

Renewable energies are highly dependent on local weather conditions, with photovoltaic energy being particularly affected by intermittent clouds. Anticipating the impact of cloud shadows on power plants is crucial, as clouds can cause partial shading, excessive irradiation, and operational issues. This study focuses on analyzing cloud tracking methods for short-term forecasts, aiming to mitigate such impacts. We conducted a systematic literature review, highlighting the most significant articles on cloud tracking from ground-based observations. We explore both traditional image processing techniques and advances in deep learning models. Additionally, we discuss current challenges and future research directions in this rapidly evolving field, aiming to provide a comprehensive overview of the state of the art and identify opportunities for significant advancements in the next generation of cloud tracking systems based on computer vision and deep learning.

关键词： Nowcasting Photovoltaic Image Processing

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ABC transporters and the hallmarks of cancer:roles in cancer aggressiveness beyond multidrug resistance

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Cancer Biology & Medicine 2020年第2期17卷 253-269页

作者： Wanjiru Muriithi Lucy Wanjiku Macharia Carlos Pilotto Heming Juliana Lima Echevarria Atunga Nyachieo paulo Niemeyer Filho Vivaldo Moura neto Instituto Estadual do Cerebro paulo Niemeyer Rio de Janeiro 20231-092Brazil Instituto de Ciencias Biomedicas Universidade Federal do Rio de JaneiroRio de Janeiro 21941-901Brazil institute of Primate Research 24481-00502 NairobiKenya Faculdade de Medicina da Universidade Federal do Rio de Janeiro Rio de Janeiro 21941-901Brazil Instituto de Microbiologia da Universidade Federal do Rio de Janeiro Rio de Janeiro 21941-901Brazil

The ATP-binding cassette transporters(ABC transporters)have been intensely studied over the past 50 years for their involvement in the multidrug resistance(MDR)phenotype,especially in *** are frequently overexpressed in both naive and post-treatment tumors,and hinder effective chemotherapy by reducing drug accumulation in cancer *** the last decade however,several studies have established that ABC transporters have additional,fundamental roles in tumor biology;there is strong evidence that these proteins are involved in transporting tumor-enhancing molecules and/or in protein-protein interactions that impact cancer aggressiveness,progression,and patient *** review highlights these studies in relation to some well-described cancer hallmarks,in an effort to re-emphasize the need for further investigation into the physiological functions of ABC transporters that are critical for tum or *** these new roles offers an opportunity to define new strategies and targets for therapy,which would include endogenous substrates or signaling pathways that regulate these proteins.

关键词： ABC transporters cancer aggressiveness hallmarks of cancer

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Concerns about the application of resistance exercise with blood-flow restriction and thrombosis risk in hemodialysis patients

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Journal of Sport and Health Science 2024年第4期13卷 548-558,F0003页

作者： Hugo de Luca Correa Lysleine Alves Deus Dahan da Cunha Nascimento Nicholas Rolnick Rodrigo Vanerson Passos Neves Andrea Lucena Reis Thais Branquinho de Araujo Carmen Tzanno-Martins Fernanda Silveira Tavares Luiz Sinesio Silva neto Claudio Avelino Rodrigues Santos Paolo Lucas Rodrigues-Silva Fernando Honorato Souza Vitoria Marra da Motta Vilalva Mestrinho Rafael Lavarini dos Santos Rosangela Vieira Andrade Jonato Prestes Thiago dos Santos Rosa Post-graduate Program of Physical Education Catholic University of BrasıliaBrasılia 71966-700Brazil The Human Performance Mechanic Lehman CollegeNew YorkNY 10011USA Clinical Group Home Dialysis Center and RenalClass Sao Paulo 01239-040Brazil Department of Medicine Catholic University of BrasıliaBrasılia 71966-700Brazil Faculty of Medicine Federal University of TocantinsTocantins 77001-090Brazil Embrapa Genetic Resources and Biotechnology Brasılia 70770-917Brazil Graduate Program of Genomic Sciences and Biotechnology Brasılia 71966-700Brazil

Background:Hemodialysis(HD) per se is a risk factor for *** the growing body of evidence on blood-flow restriction(BFR) exercise in HD patients,identification of possible risk factors related to the prothrombotic agent D-dimer is required for the safety and feasibility of this training *** aim of the present study was to identify risk factors associated with higher D-dimer levels and to determine the acute effect of resistance exercise(RE) with BFR on this ***:Two hundred and six HD patients volunteered for this study(all with a glomerular filtration rate of 70 years old),and HD ***,RE+BFR significantly increases D-dimer after 4 *** with borderline baseline D-dimer levels(400-490 ng/mL) displayed increased risk of elevating D-dimer over the normal range(≥500 ng/mL).Conclusion:These results identified factors associated with a heightened prothrombotic state and may assist in the screening process for HD patients who wish to undergo RE+BFR.D-dimer and/or other fibrinolysis factors should be assessed at baseline and throughout the protocol as a precautionary measure to maximize safety during RE+BFR.

关键词： Blood-flow restriction Chronic kidney disease Coagulation Hemodialysis Vascular occlusion exercises

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Clusters, Governance and Sustainability

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Journal of Modern Accounting and Auditing 2011年第5期7卷 509-523页

作者： paulo neto Maria Manuel Serrano University of Evora Portugal

One of the most important aspects of current economical and social reality of each local and regional territory, and very much determinative for its economic development potential, is the nature of its territorial organization of the productive processes, as well as, the characteristics, and sophistication level, of the enterprise strategies that are functioning in it. This paper seeks to contribute to the ongoing discussion on the role of clusters as engines of economic and social development and sustainability of local and regional territories. Based on a study research methodology, we intend to answer to the following research questions：（1） What is the importance of clusters in the increment process of the local and regional conditions of governance and sustainability？（2） How specific models of clusters＇ governance can contribute to greater territorial setting of enterprises and to increasing the sustainability of its development strategies？（3） To what extent industrial public policies can increase the sustainability of development solutions for local and regional economies？

关键词： collective efficiency strategies regional development clusters sustainability territorial competitiveness

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