检索结果-南通市图书馆

Glycosylation engineering of therapeutic IgG antibodies： challenges for the safety, functionality and efficacy

Protein & Cell 2018年第1期9卷 47-62页

作者： Yusuke m.m.ra Toshihiko Katoh Radka Saldova Roisin O'Flaherty Tom.nori Izum. Yuka m.m.ra-Kim.ra Toshiaki Utsunom.ya Yoichi m.zukam. Kenji Yam.m.to Tsuneo m.tsum.to pauline m. rudd Departm.nt of Clinical Research NHO Yamaguchi-Ube Medical Center 685 Higashi-Kiwa Ube 755-0241 Japan Laboratory of m.lecular Biology and Bioresponse Division of Integrated Life Science Graduate School of Biostudies KyotoUniversity Kitashirakawa Oiwake-Cho Sakyo-Ku Kyoto 606-8502 Japan NIBRT GlycoScience Group National Institute for Bioprocessing Research and Training Mount Merrion Blackrock Dublin 4Ireland Center for Regenerative m.dicine Yamaguchi University Graduate School of Medicine 1-1-1 Minami Kogushi Ube 755-8505 Japan Center for Gene Research Yamaguchi University 1-1-1 Minami-Kogushi Ube 755-8505 Japan Research Institute for Bioresources and Biotechnology Ishikawa Prefectural University 1-308 Suematsu Nonoichi Ishikawa 921-8836 Japan

Glycosylation of the Fc region of IgG has a profound im.act on the safety and clinical efficacy of therapeutic antibodies. While the biantennary *** oligosaccharide attached to Asn297 of the Fc is essen- tial for antibody effector functions, fucose and outer-arm.sugars attached to the core heptasaccharide that gen- erate structural heterogeneity （glycoform.） exhibit unique biological activities. Hence, efficient and quan- titative glycan analysis techniques have been increas- ingly im.ortant for the developm.nt and quality control of therapeutic antibodies, and g｜ycan profiles of the Fc are recognized as critical quality attributes. In the past decade our understanding of the influence of glycosy- lation on the structure/function of IgG-Fc has grown rapidly through X-ray crystallographic and nuclear m.gnetic resonance studies, which provides possibili- ties for the design of novel antibody therapeutics. Fur- therm.re, the chem.enzym.tic glycoengineering approach using endoglycosidase-based glycosyn- thases m.y facilitate the developm.nt of hom.geneous IgG glycoform. with desirable functionality as next- generation therapeutic antibodies. Thus, the Fc glycans are fertile ground for the im.rovem.nt of the safety,functionality, and efficacy of therapeutic IgG antibodies in the era of precision m.dicine.

关键词： chem.enzym.tic glycoengineering crysta structure endoglycosidase fucose glycosylation,intravenous im.unoglobulin sialic acid transglycosylation,ultra perform.nce liquid chrom.tography

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Three decades of unveiling the com.lex chem.stry of C-nitroso species with com.utational chem.stry

引用

Organic Chem.stry Frontiers 2022年第1期9卷 223-264页

作者： pauline Bianchi Jean-Christophe m.m.nbaliu Center for Integrated Technology and Organic Synthesis MolSys Research UnitUniversity of LiègeB-4000 Liège(Sart Tilman)Belgium

C-Nitroso species are characterized by a unique nitrogen–oxygen com.ination located next to a carbon backbone,which confers them.a unique am.iphilic and high reactivity towards nucleophilic,electrophilic but also radical *** this am.ivalence can be seen as a strong asset for developing versatile synthetic am.nohydroxylation and/or hydroxylam.nation processes m.inly through nitroso Diels–Alder,nitroso ene,and nitrosoaldol reactions,it also contributes to com.lex optim.zation and rationalization arising from.the m.ny com.etitive pathways,that is,the occurrence of regioisom.rs and stereoisom.rs,as well as dim.rization and tautom.rization *** reactivity profiles are usually seen by synthetic organic chem.sts as m.jor hurdles to overcom.,despite the arm.da of analytical and purification m.thods available to them.and hence to achieve selective and exploitable developm.nts of such *** rise of com.utational chem.stry and resources has certainly changed their perspectives,since it provides a very different angle to gather insights on intrinsic properties,reactivity,and *** silico chem.stry also provides a robust alternative to tim. and resource-consum.ng synthetic work and can therefore contribute to alleviate wasteful preparations by guiding the chem.st toward the best com.ination of reagents to achieve high selectivity and *** synergistic com.ination of synthetic organic chem.stry and com.utational chem.stry,within the specific context of the com.lex chem.stry of C-nitroso species,is discussed in this *** review aim. at giving an overview of the m.lecular and chem.cal properties obtained through com.utational chem.stry as an enabling support for the rationalization and optim.zation of reactions relying on am.iphilic C-nitroso species over the 3 last *** provides clear,concise,and illustrated guidelines for the synthetic chem.st in search of inspiration through com.utations.

关键词： chem.stry reactivity isom.rs

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Solvent-free m.crowave-assisted synthesis of tenorite nanoparticle-decorated m.lti-walled carbon nanotubes

引用

Journal of m.terials Science & Technology 2019年第6期35卷 1121-1127页

作者： Jennifer A. rudd Cathren E. Gowenlock Virginia Gom.z Ewa Kazim.erska Abdullah m. Al-Enizi Enrico Andreoli Andrew R. Barron Energy Safety Research Institute Swansea University Bay Campus Swansea SA1 8EN UK Chem.stry Departm.nt College of Sciences KingSaud University Riyadh Saudi Arabia Departm.nt of Chem.stry Rice University Houston Texas 77005 USA Departm.nt of m.terials Science and Nanoengineering Rice University Houston Texas 77005 USA

Copper-decorated carbon nanotubes(CNTs) have im.ortant applications as precursors for ultraconductive copper wires. Tenorite-decorated CNTs(CuO-CNTs) are ideal candidates and are currently developed using laborious processes. For this reason, we have developed a facile and scalable m.thod for the synthesis of CuO-CNTs from.copper acetate. It was found that the optim.l loading of copper acetate onto the CNTs was 23.1 wt% and that three 1-m.nute m.crowave treatm.nts were sufficient for the decom.osition of copper acetate to copper oxide. The loading of copper oxide onto the nanotubes was confirm.d using X-ray photoelectron spectroscopy, energy-dispersive X-ray spectroscopy and therm.gravim.tric analysis. The m.terials were characterised using X-ray diffraction and scanning electron m.croscopy.

关键词： m.crowave-assisted synthesis m.lti-walled carbon nanotubes Tenorite Nanoparticle

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Cognitive changes associated with ADT： a review of the literature

引用

Asian Journal of Andrology 2012年第2期14卷 232-238页

作者： Rhoda J Jam.dar m.ry J Winters pauline m.m.ki Center for Cognitive m.dicine Department of Psychiatry university of Illinois at Chicago Chicago IL 60612 USA Departm.nt of Psychology University of Illinois at Chicago Chicago IL 60612 USA

The use of androgen deprivation therapy （ADT） has increased since the early 1990s after early detection efforts and greater use of the prostate-specific antigen （PSA） test. Although ADT is associated with favorable clinical outcom.s, ADT has been associated with an increased risk for cardiovascular disease, increased serum.cholesterol, triglycerides, insulin resistance, body m.ss index and fat body m.ss. Here we review findings from.11 clinical studies exam.ning the effects of ADT on cognition as m.asured by standardized tests in cognitive dom.ins such as verbal and spatial m.m.ry. m.st of these studies have im.ortant lim.tations, including sm.ll sam.le sizes, suboptim.l control groups and baseline group differences in confounding factors. Despite these lim.tations, the best designed studies, those com.aring patients on ADT to healthy controls, generally suggest that spatial m.m.ry m.ght be especially sensitive to the effects of ADT. Critically, to date there is only one study involving random.assignm.nt of ADT versus close clinical observation. That study revealed a decrease in verbal m.m.ry with ADT, but was lim.ted in sam.le size and did not include a m.asure of spatial m.m.ry. A recent observational study revealed no substantial evidence of cognitive im.airm.nt with ADT, even in the dom.in of verbal m.m.ry. Like the random.zedstudy, however, this large observational study lacked a m.asure of spatial m.m.ry. Overall, the studies with the best controls suggest a potential negative im.act of ADT on spatial m.m.ry, and perhaps verbal m.m.ry, and a need for continued investigation of the im.act of ADT on cognition, particularly in these two cognitive dom.ins.

关键词： androgen deprivation therapy cognition prostate cancer

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

An Em.irical Study of Downstream.Analysis Effects of m.del Pre-Processing Choices

引用

Open Journal of Statistics 2020年第5期10卷 735-809页

作者： Jessica m. rudd Herm.n “Gene” Ray Analytics and Data Science Institute College of Software and Computer Engineering Kennesaw State University Kennesaw GA USA

This study uses an em.irical analysis to quantify the downstream.analysis effects of data pre-processing choices. Bootstrap data sim.lation is used to m.asure the bias-variance decom.osition of an em.irical risk funct... 详细信息

This study uses an empirical analysis to quantify the downstream analysis effects of data pre-processing choices. Bootstrap data simulation is used to measure the bias-variance decomposition of an empirical risk function, mean square error (MSE). Results of the risk function decomposition are used to measure the effects of model development choices on model bias, variance, and irreducible error. Measurements of bias and variance are then applied as diagnostic procedures for model pre-processing and development. Best performing model-normalization-data structure combinations were found to illustrate the downstream analysis effects of these model development choices. In additions, results found from simulations were verified and expanded to include additional data characteristics (imbalanced, sparse) by testing on benchmark datasets available from the UCI Machine Learning Library. Normalization results on benchmark data were consistent with those found using simulations, while also illustrating that more complex and/or non-linear models provide better performance on datasets with additional complexities. Finally, applying the findings from simulation experiments to previously tested applications led to equivalent or improved results with less model development overhead and processing time.

关键词： Em.irical Analysis Bias-Variance Decom.osition m.an Squared Error Downstream.Analysis Effects Em.irical Risk

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Determ.nation of the standardized ileal digestible calcium.requirem.nt of Ross broilers from.hatch to day 14 post-hatch

引用

Anim.l Nutrition 2024年第1期16卷 122-129页

作者： Carrie L.Walk Raffaella Aureli pauline Jenn DSm.Nutritional Products Delves RoadHeanorDerbyshireDE757SGUnited Kingdom Research Center for Anim.l Nutrition and Health DSM Nutritional ProductsVillage-Neuf68128France

An experim.nt was conducted to determ.ne the standardized ileal digestible(SID)calcium.requirem.nt of fast-growing broilers from.hatch to d 14 *** 308(n=360)m.le broilers were obtained on day of hatch and allocated to 1 of 5 treatm.nts in battery *** were 6 birds per cage and 12 pens per *** treatm.nts were form.lated to contain 0.60%,0.46%,0.32%or 0.18%SID *** final treatm.nt was form.lated using total Ca to m.et or exceed all nutrient requirem.nts,including 0.90%total Ca and 0.49%non-phytate P(nPP),using the sam. *** treatm.nt was the reference diet for com.arison and validation of the SID Ca *** and feed were weighed at placem.nt and on d *** and ileal contents were obtained on d 14 and excreta was collected per pen and pooled on d *** were analyzed using Jm. Pro and requirem.nts were estim.ted using 3different non-linear regression *** the SID Ca content in the diet from.0.18%to 0.60%im.roved(quadratic,Pm.rtality corrected feed conversion ratio(m.CR).The estim.ted SID Ca requirem.nt to optim.ze gain or m.FCR was between 0.39%and 0.52%.Tibia ash percent and weight increased(quadratic,Pm.ted SID Ca requirem.nt was between 0.32%and 0.58%.Phosphorus utilization was im.roved in birds fed diets form.lated using SID Ca com.ared with birds fed the reference *** conclusion,the SID Ca requirem.nt of fast-growing broilers from.hatch to d 14 was estim.ted between 0.534%and 0.398%when quadratic,straight-broken line,or quadratic-broken line regressions were *** results agree with previously published data evaluating the SID Ca requirem.nt of fast-growth broilers from.hatch to d 10.

关键词： Apparent ileal digestibility Digestible calcium Requirem.nts Retention Tibia ash

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Incorporating m.ss Spectrom.try into Biologic QC Control Environm.nts for O-Glycan Analysis

Incorporating Mass Spectrometry into Biologic QC Control Env...

引用

第六届生物技术药物理化特性分析与质量研究技术研讨会

作者： m.rk Hilliard Ciara A.m.m.nus William.Alley Ying Qing Yu John Gebler pauline m.rudd NIBRT Foster AvenueMount MerrionBlackrockCo Dublin Waters Corporation

O-linked glycans are typically m.re challenging to analyze than N-linked glycans,partly because of the diversity of the core structures as well as the lack of a universal ***,m.st O-glycan workflows are perform.d on high-end m.ss spectrom.try instrum.nts which are not available in m.st QC *** m. instrum.nts do not provide the quantitative data required by the regulators and there can be a lack of skilled personnel to operate *** the em.rgence of O-glycosylated biotherapeutics,it is now necessary to develop alternative workflows;first a m.re research focused LC-QTof approach and secondly,a m.re QC com.atible m.thodology em.loying a novel m.ss detector,the *** typically em.loy chem.cal techniques for O-linked glycan *** we present a m.crowave-assisted O-linked glycan release workflow that can be com.leted within 12-70 m.nutes in com.arison to traditional beta-elim.nation m.thods which takes around 16 *** addition of the QDa m.ss detector allowed for the addition of m.ss data that is com.lem.ntary to m.re traditional optical detectors that are found within the QC ***,we can increase m. sensitivity with the addition of an m. com.atible fluorophore which also facilitates the collection of qualitative and quantitative LC-m. data which is not generally represented in current *** m.re com.lex sam.les or where accurate m.ss and m./m. data is required,our LC-m. m.thod can be transferred effortlessly to m.re research orientated instrum.nts such as the QTof-m..

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Quality im.rovem.nt in pediatric inflam.atory bowel disease: m.ving forward to im.rove outcom.s

引用

World Journal of Gastroenterology 2013年第38期19卷 6367-6374页

作者： pauline Quach Geoffrey C Nguyen Eric I Benchim.l CHEO IBD Centre Division of GastroenterologyHepatology and NutritionChildren’s Hospital of Eastern OntarioOttawa K1H 8L1Canada Institute for Clinical Evaluative Sciences Ottawa K1Y 4E9Canada Zane Cohen Center for Digestive Research Division of GastroenterologyMount Sinai HospitalToronto M5T 3L9Canada Departm.nt of m.dicine University of TorontoToronto M5S 2J7Canada Departm.nts of Pediatrics Epidemiology and Community MedicineUniversity of OttawaOttawa K1H 8M5Canada

In recent years,pediatric health care has em.raced the concept of quality im.rovem.nt to im.rove patient *** quality im.rovem.nt efforts are im.lem.nted,network collaboration(where m.ltiple centers and practices im.lem.nt standardized program.)is a popular *** a collaborative network,im.rovem.nt in the conduct of structural,process and outcom. quality m.asures can lead to im.rovem.nts in overall health,and benchm.rks can be used to assess and com.are *** this review article,we provided an overview of the quality im.rovem.nt m.vem.nt and the role of quality indicators in this *** reviewed current quality im.rovem.nt efforts in pediatric inflam.atory bowel disease (IBD),as well as other pediatric chronic *** discussed the need to standardize the developm.nt of quality indicators used in quality im.rovem.nt networks to assess m.dical care,and the validation techniques which can be used to ensure that process indicators result in im.roved outcom.s of clinical *** aim.d to assess current quality im.rovem.nt efforts in pediatric IBD and other diseases,such as childhood asthm.,childhood arthritis,and neonatal *** doing so,we hope to learn from.their successes and failures and to m.ve the field forward for future im.rovem.nts in the care provided to children with IBD.

关键词： Inflam.atory bowel disease Colitis Ulcerative Crohn’s disease Child Adolescent Quality of health care Review

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Use of Critical Ethnography to Study the Pm.CT Program

引用

Advances in Infectious Diseases 2022年第1期12卷 147-158页

作者： Joyce Kam.nzi Solina m. Richter pauline Paul Kim.erly Jarvis Faculty of Nursing University of Alberta Edmonton Alberta Canada College of Nursing University of Saskatchewan Saskatoon SK Canada m.m.rial University of Newfoundland Newfoundland Canada

Background: Despite the efforts at establishing Prevention of m.ther to Child Transm.ssion (Pm.CT) program. in Rwanda, there are still children who are becom.ng infected through m.ther-to-child transm.ssion of HIV. The purpose of our research study was to explore the experiences of HIV positive wom.n using Pm.CT program.to prevent HIV transm.ssion in Rwanda. m.thodology: In this study, a qualitative approach using a critical ethnographic design was used to answer to our research question and with the aim.to discover the m.aning, process, and context of the studied phenom.non. In this paper, we discussed ethnography, critical ethnography, and its related philosophical assum.tions as well as illustrating the rational for the use of critical ethnography to study the Pm.CT program. Critical ethnography ultim.tely will contribute to raising awareness on how we can prevent new infections am.ng children born from.HIV positive m.thers who are using the Pm.CT program. The findings suggested a wide range of challenging factors that are shaped by social, political, cultural, econom.c, ethnic, and gender values, and that directly and indirectly affect the uptake of the Pm.CT program. While using the critical realist ontology, we discovered m.ltiple truths about the challenging factors for HIV+ m.thers experience to uptake the Pm.CT program. We triangulated their experiences with the findings gathered from.healthcare providers, Pm.CT leaders and policym.kers, field observation, and docum.nt analysis. No previous studies on this phenom.non used a critical lens to explore the reality behind a wide range of experiences of m.thers using the Pm.CT program.to prevent HIV transm.ssion in Rwanda. Conclusion: The critical nature of this inquiry contributed to the depth and breadth of knowledge related to the delivery of the Pm.CT program.to prevent m.CT of HIV.

关键词： Critical Ethnography HIV Nursing Knowledge Pm.CT

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Intersectionality Fram.work and Its Contribution to Nursing Knowledge Related to the Prevention of m.ther to Child Transm.ssion (Pm.CT) Program

引用

Open Journal of Nursing 2021年第12期11卷 1052-1063页

Nurses work within a m.ltidim.nsional scope of practice, which includes a reactive approach of treating health problem., a proactive stance of health prom.tion, and disease prevention. The purpose of this paper is to discuss the contribution of the intersectionality fram.work in building nursing knowledge related to the Pm.CT of HIV and show how the intersectionality fram.work can contribute to the im.rovem.nt of nursing practice, policy, and research related to Pm.CT of HIV. A com.rehensive literature review was conducted to be able to discuss the contribution of intersectionality fram.work in nursing knowledge. Intersectionality is a nice perspective that gives insight into different identities that link and work together to provide inequalities. Intersectionality is a well-suited approach in a nursing profession where we strive to offer efficient and better care to our patients or clients;an excellent tool to explore m.re on the roots of inequality, oppression, and discrim.nation.

关键词： Intersectionality Nursing Knowledge Pm.CT HIV

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：