Incorporating Mass Spectrometry into Biologic QC Control Environments for O-Glycan Analysis
作者单位：NIBRTFoster AvenueMount MerrionBlackrockCo Dublin Waters Corporation
会议名称：《第六届生物技术药物理化特性分析与质量研究技术研讨会》
会议日期：2018年
学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070305[理学-高分子化学与物理] 070302[理学-分析化学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学]
摘 要：O-linked glycans are typically more challenging to analyze than N-linked glycans,partly because of the diversity of the core structures as well as the lack of a universal ***,most O-glycan workflows are performed on high-end mass spectrometry instruments which are not available in most QC *** MS instruments do not provide the quantitative data required by the regulators and there can be a lack of skilled personnel to operate *** the emergence of O-glycosylated biotherapeutics,it is now necessary to develop alternative workflows;first a more research focused LC-QTof approach and secondly,a more QC compatible methodology employing a novel mass detector,the *** typically employ chemical techniques for O-linked glycan *** we present a microwave-assisted O-linked glycan release workflow that can be completed within 12-70 minutes in comparison to traditional beta-elimination methods which takes around 16 *** addition of the QDa mass detector allowed for the addition of mass data that is complementary to more traditional optical detectors that are found within the QC ***,we can increase MS sensitivity with the addition of an MS compatible fluorophore which also facilitates the collection of qualitative and quantitative LC-MS data which is not generally represented in current *** more complex samples or where accurate mass and MS/MS data is required,our LC-MS method can be transferred effortlessly to more research orientated instruments such as the QTof-MS.