检索结果-南通市图书馆

The biosynthetic pathway of the hallucinogen mescaline and its heterologous reconstruction

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2024年第7期17卷 1129-1150页

作者： paula Berman Luis Alejandro de Haro Ana-Rita Cavaco Sayantan Panda Younghui Dong Nikolay Kuzmich Gabriel Lichtenstein Yoav Peleg Hila Harat Adam Jozwiaki Jianghua Cai Uwe Heinig Sagit Meir Ilana Rogachev Asaph Aharoni department of Plant and Environmental Sciences Weizmann Institute of ScienceRehovot 7610001Israel Life Sciences Core Facilities Weizmann Institute of ScienceRehovot 7610001Israel The Maurice and Vivienne Wohl Institute for Drug Discovery Weizmann Institute of ScienceRehovot 7610001Israel Present address:department of Cell and Metabolic Biology Leibniz Institute of Plant BiochemistryWeinberg 306120 Halle(Saale)Germany department of Molecular&Cellular Pharmacology University of Miami Miler School of MedicineMiamiFL 33136USA department of Botany and Plant Sciences University of California RiversideRiversideCA 92521USA Laboratory of Plant Hormones and development Regulation of Chongqing School of Life SciencesChongqing UniversityChongqing 401331China

Mescaline,among the earliest identified natural hallucinogens,holds great potential in psychotherapy ***,despite the existence of a postulated biosynthetic pathway for more than half a century,the specific enzymes involved in this process are yet to be *** this study,we investigated the cactus Lophophora williamsii(Peyote),the largest known natural producer of the phenethylamine *** employed a multi-faceted approach,combining de novo whole-genome and transcriptome sequencing with comprehensive chemical profiling,enzymatic assays,molecular modeling,and pathway engineering for pathway *** identified four groups of enzymes responsible for the six catalytic steps in the mescaline biosynthetic pathway,and an N-methyltransferase enzyme that N-methylates all phenethylamine intermediates,likely modulating mescaline levels in ***,we reconstructed the mescaline biosynthetic pathway in both Nicotiana benthamiana plants and yeast cells,providing novel insights into several challenges hindering complete heterologous mescaline *** together,our study opens up avenues for exploration of sustainable production approaches and responsible utilization of mescaline,safeguarding this valuable natural resource for future generations.

关键词： mescaline Lophophora williamsi Peyote biosynthetic pathway de novo genome sequencing mo-lecular modeling

Real-World Evidence in Localized Pancreatic: Coping with Uncertainty in Unselected Populations

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Cancer Therapy 2024年第4期15卷 164-178页

作者： María I. Pamies Ramón Diana Ibarra Anguita paula Rodríguez Payá Alvaro Muñoz Abad david Sánchez García María Valero Revert Beatriz Grau Mirete Mariano Martínez Marín Alicia Calero Amaro Lorena Rodríguez Cazalla Javier Gallego Plazas Medical Oncology department Elche University Hospital Elche (Alicante) Spain General Surgery department Elche University Hospital Elche (Alicante) Spain General Surgery department Vega Baja Hospital Orihuela (Alicante) Spain

Background: Localized pancreatic cancer, including resectable (R), borderline resectable (BR) and locally advanced unresectable disease (LAU), is considered in clinical guidelines for diverse treatment options based on clinical trials in selected populations. Hence, exploring with real world evidence (RWE) clinicians’ preferences for treatment options and their results seems pertinent. Methods: In a set of consecutive patients with localized pancreatic cancer assisted in a third level hospital from January 2013 to december 2022, medical records, symptoms, diagnostic process, distribution between subtypes, and treatment plans, with safety and efficacy results, were assessed. Results: A total of 152 patients with localized disease were included (43.4% R, 21.0% BR, 33.6% LAU). The population characteristics exemplified differences between daily practice and clinical trials. Tumor location and symptoms were as expected. Treatment plan was conditioned by PS or comorbidities in 23.0% of patients. In patients with R disease, surgery followed by different adjuvant chemotherapy (CT) regimes was the antineoplastic treatment of choice (64.8%) with efficacy results (OS 37.5 months;95% CI 18.4 - 56.7), in the range of contemporary standards. The common use of neoadjuvant CT for BR disease (94.4%), with surgery in 50% of them, and its results (OS 30.8 months;95% CI 10.5 - 51.2) reflected current controversies of treatment recommendations and evolution in this scenario. Paliative CT with or without radiotherapy was the standard specific treatment in LAU disease (95.1%) with survival results (PFS: 10.8 months;95% CI 8.8 - 12.7. OS: 20.3 months;95% CI 13.5 - 27.2) that justify the distinct character and the specific study of this entity. Conclusion: RWE for localized pancreatic cancer aroused from the analysis of this population confirms the distinct nature of patients assisted in daily practice, as well as mirrors the complexity of decision making in clinical assumptions in which a

关键词： Real-World Evidence Localized Pancreatic Cancer

CECRE:Supervision and Control of Spanish Renewable Energies in the Last 15 years

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Modern Power Systems and Clean Energy 2022年第2期10卷 269-276页

作者： Gema Garcia Platero Mayte Garcia Casado Marta Perez Garcia paula Junco Madero david Alvira Baeza the Red Electrica de Espafia MaridSpain

From its commissioning in 2006 to the present,the Spanish Control Centre for Renewable Energies(CECRE)has been a worldwide pioneer and reference centre in the integration of renewable energies in the electrical *** the last 15 years,CECRE has allowed a high penetration of renewable energies in Spanish electrical system by means of the supervision and control of renewable facilities,while ensuring the power supply *** paper defines the bases of renewable en-ergy operation in CECRE and introduces forecasting systems for renewable ***,this paper describes the participation of renewable energies in balancing services and congestion ***,new challenges for Spanish transmission system operator(TSO)are presented for the integration of renewable energies along with the energy transition and decarbonization.

关键词： Balancing services controllability forecasting system observability Control Centre for Renewable Energies(CECRE) renewable energy photovoltaic(PV) wind.

Molecular detection and genetic diversity of Dientamoeba fragilis and Enterocytozoon bieneusi in fecal samples submitted for routine parasitological examination

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2021年第2期14卷 73-77页

作者： ThaináValente Bertozzo érica Boarato david Ana paula Oliveira-Arbex Semíramis Guimarães department of Parasitology Institute of BioscienceSão Paulo State University(UNESP)BotucatuSPBrazil Tropical Diseases Postgraduate Program Medical SchoolSão Paulo State University(UNESP)BotucatuSPBrazil Sagrado Coração University(USC) Department of Health SciencesBauruSPBrazil Integrated Faculties of Bauru(FIB) BauruSPBrazil

Objective:To detect Dientamoeba(D.)fragilis and Enterocytozoon(E.)bieneusi and to assess their genetic characteristics in stool samples submitted for routine examination in a clinical laboratory in Southeastern ***:In this survey,348 stool samples from female and male individuals with age ranging from 0 to≥55 years were analyzed by PCR amplifying and sequencing based on the small subunit ribosomal RNA(SSU r RNA)gene of *** and the internal transcribed spacer of ***:*** and *** isolates were observed in 2.29%(8/348)and 4.59%(16/348)of the samples,*** parasites were detected in stool samples from individuals of both genders,including young children under nine until adults over 55 years *** statistically significant differences were *** *** isolates were classified as genotype 1 and *** isolates included genotypes D(n=15)and A(n=1).Conclusions:The findings provide relevant findings on occurrence and genetic diversity of *** and ***,pointing to the need for the diagnosis of these parasites in routine examinations in clinical *** addition to sensitive diagnostic methods,it is mandatory that these parasites be considered relevant for physicians and laboratory staff.

关键词： Dientamoeba fragilis Enterocytozoon bieneusi Clinical laboratory PCR Genotyping

Implications of excreta uric acid concentrations in broilers offered reduced crude protein diets and dietary glycine requirements for uric acid synthesis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Animal Nutrition 2021年第4期7卷 939-946页

作者： Peter H.Selle david I.Cantor Leon R.McQuade Bernard V.McInerney Juliano Cesar de paula Dorigam Shemil P.Macelline Peter V.Chrystal Sonia Y.Liu Poultry Research Foundation within The University of Sydney CamdenNSW 2570Australia Sydney School of Veterinary Science The University of SydneySydneyNSW 2006Australia Australian Proteome Analysis Facility Macquarie UniversitySydneyNSW 2109Australia Evonik Nutrition and Care GmbH Hanau-Wolfgang 63547Germany School of Life and Environmental Sciences Faculty of ScienceThe University of SydneySydneyNSW 2006Australia Baiada Poultry Pty Limited Pendle HillNSW 2145Australia

In a previous experiment,male Ross 308 broiler chickens were offered dietary treatments with 3 levels of crude protein(222,193,165 g/kg)and 3 feed grains(ground maize,ground wheat,whole wheat)from 7 to 35 d ***-based diets supported superior growth performance in comparison to wheatbased *** acid concentrations in excreta were retrospectively determined and related to total nitrogen(N)excreta *** acid concentrations ranged from 28.5 to 69.4 mg/g and proportions of uric acid-N to total excreta-N ranged from 27.4%to 42.6%in broiler chickens offered the 3×3 factorial array of dietary *** of uric acid-N to total N in excreta in birds offered the165 g/kg CP,maize-based diet were significantly lower by 10.6 percentage units(27.4%versus 38.0%;P=0.00057)than their wheat-based *** excreta analysed had been collected from 35 to37 d post-hatch when feed intakes and excreta outputs were *** were linear relationships between proportions of uric acid-N to total N in excreta in birds offered the three 165 g/kg CP diets with weight gain(r=-0.587;P=0.010),feed intake(r=-0.526;P=0.025)and feed conversion ratios(r=0.635;P=0.005).The possibility that increasing uric acid-N proportions in excreta is indicative of excessive ammonia accumulations compromising growth performance is *** mean proportion of dietary glycine involved in uric acid excretion was 49.2%across all dietary treatments but ranged from 25.0%to 80.9%.Thus,the appropriate amount of dietary glycine is variable and largely dependent on the volume of uric acid synthesised and excreted.

关键词： Ammonia Broiler chicken Glycine Reduced crude protein diet Uric acid

Urban avifauna distribution explained by road noise in an Eastern European city

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Avian Research 2022年第4期13卷 445-453页

作者： Alfred-Stefan Cicort-Lucaciu Hanem-Vera Keshta paula-Vanda Popovici david Munkacsi Ilie-Catalin Telcean Carmen Gache department of Biology Faculty of Informatics and SciencesUniversity of Oradea1 Universitații Str.410087OradeaBihor CountyRomania Independent Researcher 2 Alexandru Roman Str.415200BeiușBihor CountyRomania “înv.Galgau Iosif”Secondary School 61 Pagaia417063Boianu MareBihor CountyRomania “Miskolczy Karoly”Secondary School 197 Mișca417183ChișlazBihor CountyRomania “Kazinczy Ferenc”Secondary School 602 Simian417545SimianBihor CountyRomania “Toldy”Secondary School 26 Sannicolau de Munte417441SacuieniBihor CountyRomania “Mihail Eminescu”National College 18 Roman Ciorogariu Str.410017OradeaBihor CountyRomania Technological High School No.1 48 de Jos Str.Cadea417436SacuieniBihor CountyRomania department of Biology Faculty of Biology“Alexandru Ioan Cuza”University11A Bd.Carol I Str.700505IașiIași CountyRomania

In Eastern Europe,efforts are being made for road infrastructure development,as this area has not yet reached the level of that in the *** noise negatively affects birds,especially in urban ***,seven green spaces from an Eastern European city were monitored,which were selected according to the urbanization gradient and the hydrographic peculiarities of the *** correlation between species richness and human population density,urban mobility,or road noise was *** three factors together explained over 99%of species richness distribution in habitats and during study *** noise was the strongest predictor and the most negative influencer of bird *** correlations were higher when we also considered the variability of species in habitats over time,instead of the total species richness of a habitat,as it expresses more accurately the impact of noise on the presence of *** presence of species has decreased from one habitat to another along the urbanization gradient,from the edge of the city to the center,both in the case of terrestrial and aquatic green *** most central park differed significantly from the other terrestrial green spaces in the *** the same time,no significant differences were registered between the aquatic habitats or the rest of the terrestrial *** ideal mitigation measure in this case would be that there are no roads in the immediate vicinity of green ***,if this is not possible(as in the case of all the existing green spaces in a city),we discuss/recommend placing noise barriers at the edge of green spaces or high-speed roads.

关键词： Road noise Urban green spaces Urbanization gradient

Tree succession across a seasonally dry tropical forest and forest-savanna ecotone in northern Minas Gerais,Brazil

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Journal of Plant Ecology 2017年第5期10卷 859-868页

作者： Polyanne A.Coelho Paola Ferreira Santos Eduardo de Paiva paula deborah M.G.Apgaua Bruno Gini Madeira Gisele Cristina de Oliveira Menino Yule Roberta Ferreira Nunes Rubens M.Santos david Y.P.Tng department of Forest Sciences Federal University of LavrasC.P.3037CEP 37200-000LavrasMinas GeraisBrazil department of General Biology State University of Montes ClarosC.P.126CEP 39401-089Montes ClarosMinas GeraisBrazil Instituto Federal Goiano C.P.66CEP 75901-970Rio VerdeGoiâniaBrazil Centre for Tropical Environmental and Sustainability Sciences College of Marine and Environmental SciencesJames Cook University14-88 McGregor RdSmithfield QLD 4878CairnsQueenslandAustralia

Aims Understanding succession in tropical forest is an important aspect of vegetation science,but to date,successional processes in sea-sonally dry tropical forests(SDtFs)have received much less atten-tion than evergreen humid tropical *** aim to fill this knowledge *** We investigated vegetation succession in SDtF areas consisting of three different successional stages(early,intermediate,late),and a SDtF-savanna ecotone in the municipal district of Juramento,north of Minas Gerais State,*** twelve 400 m2 plots in each area,we compared vegetation parameters and structural variables(absolute density and basal area)and examined the floristic composition of the tree component to find gradients of *** Findings We found evidence of species turnover along a successional gradi-ent,with the intermediate stage showing the highest species rich-ness and *** was accompanied with a significant increase in the number of tree individuals and basal area from the early to intermediate successional ***,the intermediate and late SDtF successional stages were more similar in structure and *** ecotone was the most species rich and was similar to the intermediate SDtF and early successional stage in species richness and floristic composition *** results will have implications for guiding SDtF management and recovery programs.

关键词： caatinga floristic Similarity ecotone forest succession resilience.

Immune strategies of phagoctytic cells stimulated in vitro with live and heat-inactivated Streptococcus pyogenes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

BIOCELL 2013年第3期37卷 63-69页

作者： Agustín César MÖHLINGER Virginia paula SIFFREDI Serenela CHULIBERT Adriana PONESSA Rodolfo david NOTARIO Diana Graciela DLUGOVITZKY Inmunology Section Microbiology DepartmentFaculty of Medical SciencesRosario National UniversityArgentina Microbiology department Faculty of Medical SciencesRosario National UniversityArgentina

Streptococcus pyogenes(group A Streptococcus)is frequently involved in a wide range of human *** we evaluated polymorphonuclear neutrophils and mononuclear cells from healthy subjects for their bactericidal function after stimulation with live and inactivated Streptococcus pyogenes(Streptococcus Group A).Mononuclear cells and Neutrophils were isolated from heparinized blood samples(n=18)using a Ficoll-Hypaque gradient and cultured in RPMI 1640 for 18 hours with a suspension of either live or inactivated Streptococcus *** the respiratory burst(flow cytometry)and nitrite,TNF and IL17 production(ELISA)were measured in the cell culture *** increased respiratory burst(expressed as R index)was induced by both live and inactivated ***,increased nitrite,TNF and IL17 concentrations were found in cell culture supernatants in both *** findings may provide some explanation as to the roles played by neutrophils and mononuclear cells in Streptococcus pyogenes immunopathogenicity。

关键词： Respiratory burst nitrites TNFa IL17

Octogenarian liver grafts:Is their use for transplant currently justified?

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2017年第17期23卷 3099-3110页

作者： Carlos Jiménez-Romero Felix Cambra Oscar Caso Alejandro Manrique Jorge Calvo Alejandro Marcacuzco paula Rioja david Lora Iago Justo HPB Surgery and Abdominal Organ Transplantation Unit University Hospital“Doce de Octubre” department of Surgery Facultad de Medicina

AIMTo analyse the impact of octogenarian donors in liver *** present a retrospective single-center study, performed between November 1996 and March 2015, that comprises a sample of 153 liver transplants. Recipients were divided into two groups according to liver donor age: recipients of donors ≤ 65 years (group A; n = 102), and recipients of donors ≥ 80 years (group B; n = 51). A comparative analysis between the groups was performed. Quantitative variables were expressed as mean values and SD, and qualitative variables as percentages. Differences in properties between qualitative variables were assessed by χ2 test. Comparison of quantitative variables was made by t-test. Graft and patient survivals were estimated using the Kaplan-Meier ***, 3 and 5-year overall patient survival was 87.3%, 84% and 75.2%, respectively, in recipients of younger grafts vs 88.2%, 84.1% and 66.4%, respectively, in recipients of octogenarian grafts (P = 0.748). One, 3 and 5-year overall graft survival was 84.3%, 83.1% and 74.2%, respectively, in recipients of younger grafts vs 84.3%, 79.4% and 64.2%, respectively, in recipients of octogenarian grafts (P = 0.524). After excluding the patients with hepatitis C virus cirrhosis (16 in group A and 10 in group B), the 1, 3 and 5-year patient (P = 0.657) and graft (P = 0.419) survivals were practically the same in both groups. Multivariate Cox regression analysis demonstrated that overall patient survival was adversely affected by cerebrovascular donor death, hepatocarcinoma, and recipient preoperative bilirubin, and overall graft survival was adversely influenced by cerebrovascular donor death, and recipient preoperative *** standard criteria for utilization of octogenarian liver grafts are: normal gross appearance and consistency, normal or almost normal liver tests, hemodynamic stability with use of < 10 μg/kg per minute of vasopressors before procurement, intensive care unit s

关键词： Older liver Donor age Marginal liver Liver transplant Aging liver