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Resistance analysis of the rice variety Huaidao 5 against root-knot nematode Meloidogyne graminicola

Journal of Integrative Agriculture 2023年第10期22卷 3081-3089页

作者： FENG Hui ZHOU Can-rong ZHU Feng LE Xiu-hu JING De-dao paul daly ZHOU Dong-mei WEI Li-hui Institute of Plant Protection Jiangsu Academy of Agricultural SciencesNanjing 210014P.R.China Plant Protection and Quarantine Station of Jiangsu Province Nanjing 210009P.R.China School of Landscape and Ecological Engineering Hebei University of EngineeringHandan 056038P.R.China Zhenjiang Institute of Agricultural Science in Hilly Area of Jiangsu Province Jurong 212400P.R.China

Meloidogyne graminicola has emerged as one of the most destructive plant-parasitic nematodes affecting rice(Oryza sativa)production *** to *** in rice could be the most effective option for its ***,sources of germplasm with resistance to *** in rice remain ***,we describe the root attraction,gall formation and genetic analysis of the resistance to *** in the rice variety Huidao 5.A nematode attraction assay showed that second-stage juveniles(J2s)of *** were attracted at the root tip of Huaidao 5 within 8 h without a significant reduction in attraction compared to the susceptible rice variety Nanjing *** observation of the infection revealed that the J2s invaded root tissues 12 h after inoculation,but their subsequent movement to the root tip was hindered in Huaidao 5,resulting in decreased nematode number compared to Nanjing ***,we used the soil and hydroponic culture systems to simulate upland and flooding conditions in the paddy fields respectively,and found that gall number was significantly reduced,and nematode development was clearly suppressed in Huaidao *** investigate the genetic basis of this resistance,cross breeding was performed between the Huaidao 5 and Nanjing 9108 *** was no reduction in the resistance of the F_(1) offspring to *** in the greenhouse or field trials,suggesting that a dominant gene could control resistance in Huaidao *** summary,this study provides a detailed characterization of a novel source of resistance to *** in rice,which is of great potential for use in crop breeding.

关键词： root-knot nematode Meloidogyne graminicola rice resistance

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Interaction Between the Ginger Soft Rot Pathogen Pythium myriotylum and the Biocontrol Agent Pythium oligandrum

Interaction Between the Ginger Soft Rot Pathogen Pythium myr...

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中国植物病理学会2023年学术年会

作者： paul daly Taha Majid Mahmood Sheikh ZHOU Dongmei ZHANG Jinfeng CHEN Siqiao DENG Sheng Irina Druzhinina WEI Lihui Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base Institute of Plant ProtectionJiangsu Academy of Agricultural Sciences Jiangsu Provincial Key Lab of Organic Solid Waste Utilization Fungal Genomics GroupNanjing Agricultural University School of Environment and Safety Engineering Jiangsu University

The use of natural antagonistic microorganisms, such asPythium oligandrum, is a promising environmentally friendly approach to biologically control Pythium soft-rot disease of ginger(Zingiber officinale) caused by Pythium myriotylum.A critical part to improve the efficiency of biocontrol applications is to understand both the mechanisms of action of ***, and the mechanisms by which *** *** antagonism involves hydrolytic enzymes such as cellulases and proteases, and recently we uncovered a role for *** — produced volatile organic compounds in antagonism of *** effects of these mechanisms are obvious from major morphological and cytological damage to *** of the response of *** to *** proteases appears to involve upregulation of protease ***, in response to *** volatile compounds,*** initially showed a strong upregulation of putative detoxification-related genes that was not maintained at later ***, the responses of *** to *** suggest ways to reduce the ability of *** to defend against and counter-antagonise ***( e.g., by targeting *** protease inhibitors or detoxification genes), and thus improve the efficiency of ***-mediated ginger disease control.

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Insights into the broad-host range plant pathogen Pythium myriotylum（Oomycota）and biocontrol of soft-rot disease of ginger

Insights into the broad-host range plant pathogen Pythium my...

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中国植物病理学会2021年学术年会

作者： paul daly ZHOU Dongmei SHEN Danyu ZHANG Qimeng CHEN Siqiao XUE Taiqiang CHEN Yifan Jamie McGowan David Fitzpatrick DENG Sheng LI Jingjing Irina DRUZHININA WEI Lihui Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base Institute of Plant ProtectionJiangsu Academy of Agricultural Sciences College of Plant Protection Nanjing Agricultural University Jiangsu Provincial Key Lab of Organic Solid Waste Utilization Fungal Genomics GroupNanjing Agricultural University School of Environment and Safety Engineering Jiangsu University Genome Evolution Laboratory Maynooth UniversityCo.Kildare

Pythium myriotylum is a major pathogen infecting ginger（Zingiber officinale） rhizomes in China with symptoms of Pythium soft rot（PSR） *** is an important crop with global production estimated at approximately three million metric tonnes with about 20% of this production in *** genome of a relatively highly virulent *** strain isolated from an infected ginger rhizome was *** genome encodes one of the largest repertoires of pathogenesis-related genesamongst Pythium species,and the expansion in these genes appears to be due to tandem *** the putative effectors,a subset of the NEP1-like toxin protein（NLP） effectors was highly upregulated during the infection of ginger leaves,and can induce necrosis in *** *** oomycete biocontrol agent Pythium oligandrum as well as Trichoderma *** control PSR of ginger caused by ***-transcriptomic analysis of the interaction between *** and highlighted potential defensive responses of *** such as the upregulation of protease inhibitors and *** genome highlights the potential of the large repertoire of pathogenesis-related genes to contribute to the broad host range of ***,and biocontrol by *** of PSR of ginger is a promising strategy.

关键词： PSR Pythium myriotylum Pythium oligandrum Genome

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Exercise attenuates bone mineral density loss during diet-induced weight loss in adults with overweight and obesity:A systematic review and meta-analysis

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Journal of Sport and Health Science 2021年第5期10卷 550-559页

作者： Jakub Mesinovic paul Jansons Ayse Zengin Barbora de Courten Alexander J.Rodriguez Robin M.daly Peter R.Ebeling David Scott Department of Medicine School of Clinical Sciences at Monash HealthMonash UniversityClaytonVIA 3168Australia Institute for Physical Activity and Nutrition(IPAN) School of Exercise and Nutrition SciencesDeakin UniversityBurwoodVIA 2134Australia School of Medical and Health Sciences Edith Cowan UniversityPerthWA 6027Australia Department of Medicine and Australian Institute of Musculoskeletal Science Melbourne Medical SchoolWestern CampusUniversity of MelbourneSt AlbansVIA 3021Australia

Background:Weight-loss-induced fat loss improves cardiometabolic health in individuals with overweight and obesity;however,weight loss can also result in bone loss and increased fracture ***-loss-induced bone loss may be attenuated with *** aim was to compare changes in bone mineral density(BMD)in adults with overweight and obesity who undertook diet-induced weight loss alone or in combination with ***:We included randomized controlled trials(RCTs)in adults with overweight or obesity(aged-18 years;body mass index-25 kg/m^(2))that prescribed diet-induced weight loss alone or in combination with supervised exercise,and measured any bone structural *** of bias was assessed using the Cochrane Risk of Bias ***-effects meta-analyses determined mean changes and net mean differences(95%confidence intervals(95%CIs))in the percentage of areal BMD(aBMD)change between ***:We included 9 ***-induced weight loss led to significant losses in femoral neck aBMD(mean change:-1.73%(95%CI:-2.39%to-1.07%),p<0.001)and total hip aBMD(-2.19%(95%CI:-3.84%to-0.54%),p=0.009).Femoral neck aBMD losses were significantly greater in the diet-induced weight loss group compared to the exercise plus diet-induced weight loss group(net difference:-0.88%(95%CI:-1.73%to-0.03%));however,there were no differences in aBMD changes at any other skeletal site:total hip(-1.96%(95%CI:-4.59%to 0.68%))and lumbar spine(-0.48%(95%CI:-1.81%to 0.86%)).aBMD changes did not differ significantly according to exercise modality(resistance exercise,aerobic exercise,or a combination of the two)during diet-induced weight ***:Diet-induced weight loss led to greater femoral neck bone loss compared to diet-induced weight loss plus *** loss at the total hip and lumbar spine was not attenuated by exercise during diet-induced weight *** lack of consistent skeletal benefits may be due to the insufficient duration and/or training intensities

关键词： Bone mass Exercise Obesity Overweight Weight loss

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Comparison of Humoral Immune Responses Elicited by DNA and Protein Vaccines Based on Merozoite Surface Protein-1 from Plasmodium yoelii,a Rodent Malaria Parasite

Comparison of Humoral Immune Responses Elicited by DNA and P...

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作者： paul A．Calvo Thomas M．daly Carole A．Long Department of Microbiology and Immunology Allegheny University of the Health SciencesPhiladelphiaPA 19129USA

Immunization with DNA vaccines encoding relevant Ags can induce not only cell-mediated immune response but also humoral immune responses against pathogenic microorganisms in several animal models. Our previous results demonstrated that,when the C terminus (PyC2) of Plasmodium yoelii merozoite surface protein-1 (MSP-1).a leading vaccine candidate against erythrocytic stages of malaria, was expressed as a fusion protein (GST-PyC2) with glutathione S-transferase (GST), it elicited Ab-mediated protective immune responses in BALB/c mice. In our present study, we wished to examine

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Design of Tokamak ELM Coil Support in High Nuclear Heat Environment

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Plasma Science and Technology 2014年第3期16卷 300-304页

作者：张善文宋云涛王忠伟戢翔 E.daly M.KALISH 卢速杜双松刘旭峰冯昌乐杨洪王松可 Institute of Plasma Physics Chinese Academy of Sciences ITER Organization Route de Vinonsur Verdon13115 Saint Paul-lez-DuranceFrance Princeton Plasma Physics Laboratory

In Tokomak, the support of the ELM coil, which is close to the plasma and subject to high radiation level, high temperature and high magnetic field, is used to transport and bear the thermal load due to thermal expansion and the alternating electromagnetic force generated by high magnetic field and AC current in the coil. According to the feature of ITER ELM coil, the mechanical performance of rigid and flexible supports under different high nuclear heat levels is studied. Results show that flexible supports have more excellent performance in high nuclear heat condition than rigid supports. Concerning thermal and electromagnetic （EM） loads, optimized results further prove that flexible supports have better mechanical performance than rigid ones. Through these studies, reasonable support design can be provided for the ELM coils or similar coils in Tokamak based on the nuclear heat level.

关键词： tokomak ELM coil rigid support flexible support high nuclear heat

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α-芋螺毒素LtIA结构与功能的研究

α-芋螺毒素LtIA结构与功能的研究

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第九届中国生物毒素学术研讨会

作者：罗素兰 Kalyana Bharati Akondi 长孙东亭吴勇朱晓鹏胡远艳 Sean Christensen Cheryl Dowell Norelle L.daly David J.Craik Ching-I Anderson Wang Richard J.Lewis paul F.Alewood J.Michael McIntosh 热带生物资源教育部重点实验室(海南大学)

＜正＞具有重要生理作用和临床研究意义的烟碱乙酰胆碱受体（nAChRs）介导众多中枢和外周神经系统的生理功能,包括学习、记忆、应答、镇痛和运动控制等。nAChRs激活多巴胺、去甲肾上腺素、五羟色胺、γ-氨基丁酸等多种神经递质的释放。...

＜正＞具有重要生理作用和临床研究意义的烟碱乙酰胆碱受体（nAChRs）介导众多中枢和外周神经系统的生理功能,包括学习、记忆、应答、镇痛和运动控制等。nAChRs激活多巴胺、去甲肾上腺素、五羟色胺、γ-氨基丁酸等多种神经递质的释放。已证实nAChRs是筛选诊断和治疗一大类重要疾病药物的关键靶点,这些疾病包括智障、成瘾、疼痛、帕金森症、精

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Nature：大规模研究揭示生命所必需的基因

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现代生物医学进展 2016年第31期16卷 I0002-I0003页

作者： Dickinson, Mary E. Flenniken, Ann M. Ji, Xiao Teboul, Lydia Wong, Michael D. White, Jacqueline K. Meehan, Terrence F. Weninger, Wolfgang J. Westerberg, Henrik Adissu, Hibret Baker, Candice N. Bower, Lynette Brown, James M. Caddle, L. Brianna Chiani, Francesco Clary, Dave Cleak, James daly, Mark J. Denegre, James M. Doe, Brendan Dolan, Mary E. Edie, Sarah M. Fuchs, Helmut Gailus-Durner, Valerie Galli, Antonella Gambadoro, Alessia Gallegos, Juan Guo, Shiying Horner, Neil R. Hsu, Chih-Wei Johnson, Sara J. Kalaga, Sowmya Keith, Lance C. Lanoue, Louise Lawson, Thomas N. Lek, Monkol Mark, Manuel Arschall, Susan M. Mason, Jeremy McElwee, Melissa L. Newbigging, Susan Nutter, Lauryl M. J. Peterson, Kevin A. Ramirez-Solis, Ramiro Rowland, Douglas J. Ryder, Edward Samocha, Kaitlin E. Seavitt, John R. Selloum, Mohammed Szoke-Kovacs, Zsombor Tamura, Masaru Trainor, Amanda G. Tudose, Ilinca Wakana, Shigeharu Warren, Jonathan Wendling, Olivia West, David B. Wong, Leeyean Yoshiki, Atsushi MacArthur, Daniel G. Tocchini-Valentini, Glauco P. Gao, Xiang Flicek, paul Bradley, Allan Skarnes, William C. Justice, Monica J. Parkinson, Helen E. Moore, Mark Wells, Sara Braun, Robert E. Svenson, Karen L. de Angelis, Martin Hrabe Herault, Yann Mohun, Tim Mallon, Ann-Marie Henkelman, R. Mark Brown, Steve D. M. Adams, David J. Lloyd, K. C. Kent McKerlie, Colin Beaudet, Arthur L. Bucan, Maja Murray, Stephen A. Dept Mol Physiol & Biophys Houston TX 77030 USA Toronto Ctr Phenogen Toronto ON M5T 3H7 Canada Mt Sinai Hosp Toronto ON M5G 1X5 Canada Univ Penn Perelman Sch Med Genom & Computat Biol Program Philadelphia PA 19104 USA Med Res Council Harwell Mammalian Genet Unit Harwell OX11 0RD Oxon England Mary Lyon Ctr Harwell OX11 0RD Oxon England Hosp Sick Children Mouse Imaging Ctr Toronto ON M5T 3H7 Canada Wellcome Trust Sanger Inst Wellcome Trust Genome Campus Cambridge CB10 1SA England European Bioinformat Inst European Mol Biol Lab Wellcome Trust Genome Campus Cambridge CB10 1SD England Med Univ Vienna Ctr Anat & Cell Biol A-1090 Vienna Austria Hosp Sick Children 555 Univ Ave Toronto ON M5G 1X8 Canada Jackson Lab 600 Main St Bar Harbor ME 04609 USA Univ Calif Davis Mouse Biol Program Davis CA 95618 USA Italian Natl Res Council CNR Inst Cell Biol & Neurobiol Monterotondo Mouse Clin I-00015 Monterotondo Italy Massachusetts Gen Hosp Analyt & Translat Genet Unit Boston MA 02114 USA Broad Inst MIT & Harvard Program Med & Populat Genet Cambridge MA 02142 USA German Res Ctr Environm Hlth Helmholtz Zentrum Munchen Inst Expt Genet D-85764 Neuherberg Germany German Mouse Clin D-85764 Neuherberg Germany Baylor Coll Med Dept Mol & Human Genet Houston TX 77030 USA Nanjing Univ Nanjing Biomed Res Inst Collaborat Innovat Ctr Genet & Dev SKL Pharmaceut Biotechnol & Model Anim Res Ctr Nanjing 210061 Jiangsu Peoples R China Univ Strasbourg ICS Infrastruct Natl PHENOMIN F-67404 Illkirch Graffenstaden France Univ Strasbourg INSERM CNRS IGBMC F-67404 Illkirch Graffenstaden France RIKEN BioResource Ctr Tsukuba Ibaraki 3050074 Japan Childrens Hosp Oakland Res Inst Oakland CA 94609 USA IMPC San Anselmo CA 94960 USA Tech Univ Munich Sch Life Sci Weihenstephan Chair Expt Genet D-81675 Freising Weihenstephan Germany German Ctr Diabet Res DZD D-85764 Neuherberg Germany Francis Crick Inst Mill Hill Lab Mill Hill London NW1 1AT England Univ Penn Perlman

在哺乳动物基因组中。大约三分之一的基因是生命所必需的。在一项新的研究中，来自美国贝勒医学院等多个机构的研究人员描述了这些基因的大规模发现。以及它将如何影响对哺乳动物发育和人类疾病的理解。相关研究结果在线发表在Nature期... 详细信息

在哺乳动物基因组中。大约三分之一的基因是生命所必需的。在一项新的研究中，来自美国贝勒医学院等多个机构的研究人员描述了这些基因的大规模发现。以及它将如何影响对哺乳动物发育和人类疾病的理解。相关研究结果在线发表在Nature期刊上，论文标题为“High—throughput discovery of novel developmental phenotypes”。

关键词：动物基因组生命哺乳动物研究人员人类疾病医学院

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