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*** stress induced in retinal pigmented epithelium cells by the synergy between polycyclic aromatic hydrocarbons and blue light

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Annals of Eye Science 2018年第1期 450-450页

作者： Corinne Zinflou patrick j.rochette Axe Médecine Régénératrice Centre de Recherche du CHU de QuébecUniversitéLavalHôpital du Saint-SacrementQuébecQCCanada Centre de Recherche en Organogénèse Expérimentale de l'UniversitéLaval/LOEX UniversitéLavalQuébecQCCanada Département d’Ophtalmologie et ORL-chirurgie cervico-faciale UniversitéLavalQuébecQCCanada

Background:Lesion to the retinal pigment epithelium(RPE)is a crucial event in age-related macular degeneration(AMD)*** the pathogenesis of this complex disease is poorly understood,sunlight exposure and smoking are major environmental risk factors associated with ***-energy visible blue light(HEV;400-500 nm)is the most energetic and potentially harmful solar wavelengths reaching adults *** the other hand,RPE cells can be exposed to a large range of pollutants from cigarette smoke,with polycyclic aromatic hydrocarbons(PAH)being among the most *** PAH from cigarette smoke can absorb HEV *** led us hypothesize that in RPE cells,the combination of PAH and HEV could synergize to exacerbate the stress caused by either factor *** thus investigate the combined effect of PAH and HEV light in RPE ***:Confluent RPE immortalized cells(ARPE19)were exposed to nanomolar concentrations of benzo[a]pyrene(BaP)or indeno[1,2,3-cd]pyrene(IcdP).While IcdP efficiently absorbs HEV wavelengths,BaP,the most studied PAH,does not significantly absorb HEV light and was used as a *** or IcdP contaminated ARPE19 were then irradiated with increasing sub-lethal doses of HEV light(150-500 j/cm2)using a setup that mimics the light spectrum normally reaching the ***,apoptosis and reactive oxygen species(ROS)generation were assessed in each ***:In presence of low concentrations of IcdP,sub-lethal amounts of HEV light trigger,in a dose-dependent way,up to 70%of apoptotic cell ***-exposure to IcdP and HEV also leads to a synergistic ROS generation in ARPE19 cells,thus inducing oxidative *** of these effects were observed with *** inhibition of ROS production by specific antioxidants only decreases death by 20%in cells simultaneously exposed to both IcdP and HEV ***:Low concentrations of IcdP synergize with HEV light to induce phototoxicity in ARPE19 *** increased oxidat

关键词： Retinal pigment epithelium(RPE) polycyclic aromatic hydrocarbons(PAH) blue light oxidative stress age-related macular degeneration(AMD)

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***-oxidation of N-retinylidene-N-retinylethanolamine in vitro by high-energy visible light

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Annals of Eye Science 2018年第1期 430-430页

作者： Marie-Christine Lambert Mathieu Ouellette Élodie Boisselier patrick j.rochette Centre Universitaire d’Ophtalmologie Hôpital du Saint-SacrementCHU de QuébecCanada Axe Médecine Régénératrice Centre de recherche du CHU de QuébecHôpital du Saint-SacrementCHU de QuébecCanada Département d’ophtalmologie et d’ORL-CCF Facultéde médecineUniversitéLavalQuébecCanada

Background:Age-related macular degeneration(AMD)is the second Canadian cause in visual *** is characterized by the death of photoreceptors and retinal pigmented epithelium(RPE)in the macular region of the retina,leading to the loss of central *** studies suggest an association between lifetime sun exposure and the probability to develop AMD even though mechanisms are *** is made of about 30%of high-energy visible(HEV)light(blue light),the most energetic wavelength reaching the *** wavelengths can be absorbed by lipofuscin,an age pigment accumulating in RPE *** principal component is N-retinylidene-N-retinylethanolamine(A2E).Many research teams showed that absorption of HEV light by A2E in RPE cells at non-physiological doses produces free radicals and leads to cell *** earlier work shows that when A2E-loaded RPE cells are irradiated with HEV light at physiological doses,the same light does not lead to oxidative stress as measured by telomere and mitochondrial *** hypothesis is that HEV light,at physiological doses,modify or convert A2E in derived produces,inhibiting its photo-oxidant ***:In vitro,we irradiated A2E with HEV light with or without antioxidants and with varying irradiation regimen to observe the UV-Visible spectrum of *** cellulo,we loaded ARPE-19 cells with A2E and irradiated cells at physiological levels for 4 consecutive *** then observed A2E fluorescence using a fluorescence microscope with nucleus counterstaining with ***:HEV light leads to the disappearance of A2E characteristic UV-Visible spectrum and the apparition of a new product suggesting that HEV light modifies *** oxidation and irradiation regimen seem to have an impact in A2E’s conversion by HEV *** observed and progressive diminution of A2E fluorescence in cellulo during physiological ***:The loss of A2E photo-oxidation capacities by HEV light seems to

关键词： Age-related macular degeneration(AMD) A2E degradation blue light retinal pigmented epithelium(RPE) cellular toxicity

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*** database of clinical data and biological material for research on uveal melanoma

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Annals of Eye Science 2018年第1期 453-453页

作者： Solange Landreville Frédéric Mouriaux patrick j.rochette Christian Salesse Mohib W.Morcos Claudine Bellerive Sylvain Guérin Centre de recherche du CHU de Québec-UniversitéLaval Hôpital du Saint-SacrementQuébecQCCanada Département d’ophtalmologie et d’ORL-CCF Facultéde médecineUniversitéLavalQuébecQCCanada Centre de recherche en organogénèse expérimentale de l’UniversitéLaval/LOEX QuébecQCCanada Département d'ophtalmologie Facultéde médecineUniversitéde Rennes 1RennesFrance Service d’ophtalmologie CHU de RennesRennesFrance

Background:Sharing biological material and clinical data from patients with uveal ***:Uveal melanoma is the most common intraocular malignancy in the adult *** uveal melanoma is primarily a sporadic cancer and familial cases are rare,it is difficult to prevent or detect *** effective treatment of ocular tumors,more than 50%of patients develop incurable liver metastases mainly in the 5-10 years following the detection of the primary *** cancer is relatively rare and the obtained biopsies are very *** 20 samples are taken each year in *** provincial infrastructure is made of biological material from donors with uveal melanoma and a large clinical *** tumor biopsies are used for culturing cell lines and the creation of a DNA/RNA library used for genomic and genetic ***:This infrastructure plays an important role in the achievement of various research programs for a better understanding of genetic and environmental factors involved in the development of melanoma and the spread of *** allows collaboration with other researchers at a provincial,national and international level in order to make progress in basic and clinical research on uveal ***:The biological material and clinical data of this infrastructure are available upon request to VHRN members whose research project was approved by the ethics committee of the institution.

关键词： Uveal melanoma tumor biopsies blood clinical data

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Porous Indium Nanocrystals on Conductive Carbon Nanotube Networks for High-Performance CO2-to-Formate Electrocatalytic Conversion

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Energy & Environmental Materials 2024年第4期7卷 413-421页

作者： Liangping Xiao Rusen Zhou Tianqi Zhang Xiaoxiang Wang Renwu Zhou patrick j.Cullen Kostya (Ken) Ostrikov Department of Physics Research Institute for Biomimetics and Soft Matter Fujian Provincial Key Laboratory for Soft Functional Materials Jiujiang Research Institute Xiamen University School of Chemistry and Physics QUT Centre for Materials Science and QUT Centre for a Waste-Free World Queensland University of Technology(QUT) School of Chemical and Biomolecular Engineering University of Sydney

Ever-increasing emissions of anthropogenic carbon dioxide （CO2） cause global environmental and climate *** by biological photosynthesis,developing effective strategies NeuNlto up-cycle CO2into high-value organics is *** CO2reduction reaction （CO2RR） is highly promising to convert CO2into economically viable carbon-based chemicals or fuels under mild process conditions.

关键词： CO2RR conductive network electrocatalysts formate

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Stand dynamics of old-growth hemlock forests in central Bhutan are shaped by natural disturbances

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Forest Ecosystems 2024年第4期11卷 434-447页

作者： Karma Tenzin Craig R.Nitschke Kathryn j.Allen Raphael Trouve Thiet V.Nguyen patrick j.Baker School of Agriculture Foodand Ecosystem SciencesUniversity of Melbourne500 Yarra BlvdRichmondVictoriaAustralia School of Geography Planningand Spatial SciencesUniversity of TasmaniaSandy BayTasmaniaAustralia Forest Science Institute of South Vietnam 1 Pham Van Hai StreetTan Binh DistrictHo Chi Minh CityViet Nam

Understanding how past disturbances have influenced the development of forests is critical for deciphering their current structure and composition and forecasting future *** this study,dendrochronological methods were applied to uncover the disturbance history of old-growth hemlock-dominated forests in central *** of tree-ring samples from two old-growth hemlock stands,located in two different topographic settings,identified the importance of gap-phase dynamics in facilitating recruitment and growth releases and producing complex,multi-aged structure s over *** site showed evidence of a near stand-replacing disturbance in the late 1700s,while the other showed no evide nce of high-severity disturbance at any time over the last 400 *** both sites low-to medium-severity disturbances,some of which appear to be associated with cyclones originating in the Bay of Bengal,dominated the disturbance *** hemlock stands exhibited a significant positive association between cyclone occurrence and growth release events and between recruitment pulses and growth release *** 1800 to 1970 there was an increase in recruitment of angiosperm tree species at most sites and a corresponding decline in conifer *** the past 50 years there has been little new recruitment;this may be due to light limitation in the understory from shade-tolerant angiosperms and bamboo in the lower strata of these *** variations in disturbance dynamics and recruitment were observed across the study sites,suggesting that other factors,such as topography and climate,may be influencing long-term stand development *** study highlights the complex interplay between historical disturbance regimes and tree recruitment in shaping the age and size structures of old-growth hemlock forests in central *** also provides new insights into the dynamics of these forests that can be used to support effective forest conservation and management i

关键词： Forest structure Hemlock forest Himalaya Natural disturbance

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Opportunities for large language models and discourse in engineering

Energy and AI

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Energy and AI 2024年第3期17卷 136-142页

作者： jan Göpfert jann M.Weinand patrick Kuckertz Detlef Stolten Institute of Energy and Climate Research–Techno-economic Systems Analysis(IEK-3) Forschungszentrum Jülich GmbHJülich52425Germany Chair for Fuel Cells RWTH Aachen UniversityFaculty of Mechanical EngineeringAachen52062Germany

In recent years, large language models have achieved breakthroughs on a wide range of benchmarks in natural language processing and continue to increase in performance. Recently, the advances of large language models have raised interest outside the natural language processing community and could have a large impact on daily life. In this paper, we pose the question: How will large language models and other foundation models shape the future product development process? We provide the reader with an overview of the subject by summarizing both recent advances in natural language processing and the use of information technology in the engineering design process. We argue that discourse should be regarded as the core of engineering design processes, and therefore should be represented in a digital artifact. On this basis, we describe how foundation models such as large language models could contribute to the design discourse by automating parts thereof that involve creativity and reasoning, and were previously reserved for humans. We describe how simulations, experiments, topology optimizations, and other process steps can be integrated into a machine-actionable, discourse-centric design process. As an example, we present a design discourse on the optimization of wind turbine blades. Finally, we outline the future research that will be necessary for the implementation of the conceptualized framework.

关键词： Product development process Conceptual design Design methodology Design generation Natural language processing Foundation models Multi-modal models

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Inhaled volatile anesthetics in the intensive care unit

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World journal of Critical Care Medicine 2024年第1期13卷 28-39页

作者： Erin D Wieruszewski Mariam ElSaban patrick M Wieruszewski Nathan j Smischney Department of Pharmacy Mayo ClinicRochesterMN 55905United States Anesthesiology and Perioperative Medicine Mayo ClinicRochesterMN 55905United States Department of Anesthesiology&Perioperative Medicine Mayo ClinicRochesterMN 55905United States

The discovery and utilization of volatile anesthetics has significantly transformed surgical practices since their inception in the mid-19th ***,a paradigm shift is observed as volatile anesthetics extend beyond traditional confines of the operating theatres,finding diverse applications in intensive care *** the dynamic landscape of intensive care,volatile anesthetics emerge as a promising avenue for addressing complex sedation requirements,managing refractory lung pathologies including acute respiratory distress syndrome and status asthmaticus,conditions of high sedative requirements including burns,high opioid or alcohol use and neurological conditions such as status *** anesthetics can be administered through either inhaled route via anesthetic machines/devices or through extracorporeal membrane oxygenation circuitry,providing intensivists with multiple options to tailor ***,their unique pharmacokinetic profiles render them titratable and empower clinicians to individualize management with heightened accuracy,mitigating risks associated with conventional sedation *** the amounting enthusiasm for the use of these therapies,barriers to widespread utilization include expanding equipment availability,staff familiarity and training of safe *** article delves into the realm of applying inhaled volatile anesthetics in the intensive care unit through discussing their pharmacology,administration considerations in intensive care settings,complication considerations,and listing indications and evidence of the use of volatile anesthetics in the critically ill patient population.

关键词： Anesthesia Critical care Mechanical ventilation Sedation Volatile anesthetics Sedative

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A novel diagnostic gene region for distinguishing between two pest fruit flies:Bactrocera tryoni(Froggatt)and Bactrocera neohumeralis(Hardy)(Diptera:Tephritidae)

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Insect Science 2024年第4期31卷 1285-1295页

作者： Melissa L.Starkie Elizabeth V.Fowler Alexander M.Piper Xiaocheng Zhu Pauline Wyatt David Gopurenko Matt N.Krosch Francesca Strutt Karen F.Armstrong Hamish patrick Mark K.Schutze Mark j.Blacket Department of Agriculture and Fisheries BiosecurityBrisbaneQueenslandAustralia Agriculture Victoria AgriBioBundooraVictoriaAustralia Department of Primary Industries Wagga Wagga Agricultural InstituteWagga WaggaNew South WalesAustralia Forensic Services Group Queensland Police ServiceBrisbaneQueenslandAustralia Department of Pest-management and Conservation Lincoln UniversityLincolnNew Zealand Better Border Biosecurity(B3 B3nz.org.nz)LincolnNew Zealand

Bactrocera tryoni and Bactrocera neohumeralis are morphologically similar sibling pest fruit fly species that possess different biological attributes,geographic distributions,and host *** need to differentiate between the two species is critical for accurate pest status assessment,management,biosecurity,and maintenance of reference *** morphologically similar,adults may be separated based on subtle characters;however,some characters exhibit intraspecific variability,creating overlap between the two ***,there is currently no single molecular marker or rapid diagnostic assay that can reliably distinguish between *** and ***;therefore,ambiguous samples remain *** we report the first molecular marker that can consistently distinguish between *** and *** diagnostic region consists of two adjacent single nucleotide polymorphisms(SNPs)within the pangolin(pan)gene *** confirmed the genotypes of each species are consistent across their distributional range,then developed a tetra-primer amplification refractory mutation system(ARMS)PCR assay for rapid diagnosis of the *** assay utilizes four primers in multiplex,with two outer universal primers,and two internal primers:one designed to target two adjacent SNPs(AA)present in *** and the other targeting adjacent SNPs present in ***(GG).The assay accurately discriminates between the two species,but their SNP genotypes are shared with other nontarget tephritid fruit fly ***,this assay is most suited to adult diagnostics where species confirmation is necessary in determining ambiguous surveillance trap catches;maintaining pure colony lines;and in Sterile Insect Technique management responses.

关键词： ARMS-PCR colony molecular diagnostics pangolin tetra-primer

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An oxygenating colloidal bioink for the engineering of biomimetic tissue constructs

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Bio-Design and Manufacturing 2024年第3期7卷 240-261页

作者： Seol-Ha jeong jarno Hiemstra patrick V.Blokzijl Rebeca Damian-Ferrara Danilo Martins dos Santos jéssica H.L.da Fonseca Min-Ho Kang jihyun Kim Dilara Yilmaz-Aykut Mei L.L.Cham-Pérez jeroen Leijten Su Ryon Shin Division of Engineering in Medicine Department of MedicineBrigham and Women’s HospitalHarvard Medical SchoolBostonMA 02139USA Leijten Lab Department of Developmental BioEngineeringFaculty of Science and TechnologyTechnical Medical CentreUniversity of TwenteDrienerlolaan 57522 NB EnschedeThe Netherlands Nanotechnology National Laboratory for Agriculture(LNNA) Embrapa InstrumentaçãoSao CarlosSao Paulo 13560-970Brazil Department of Biomedical-Chemical Engineering The Catholic University of Korea43 Jibong-roBucheon-siGyeonggi-do 14662Republic of Korea Department of Biotechnology The Catholic University of Korea43 Jibong-roBucheon-siGyeonggi-do 14662Republic of Korea

Ensuring a sufficient oxygen supply is pivotal for the success of bioprinting applications since it fosters tissue integration and natural *** in oxygen concentration among diverse tissues necessitates the precise recreation of tissue-specific oxygen levels in imprinted constructs to support the survival of targeted *** oxygen-releasing biomaterials,such as oxygen-generating microparticles(OMPs),have shown promise for enhancing the oxygen supply of microenvironments in injured tissues,whether this approach is scalable for large tissues and whether tissue-specific bioinks with varying OMP concentrations remain printable remain *** study addresses this critical gap by introducing an innovative class of engineered oxygenated bioinks that combine colloidal-based microgels with *** report that incorporating nanosized calcium peroxide(nCaO_(2))and manganese oxide nanosheets(nMnO_(2))into hydrophobic polymeric microparticles enables precise modulation of oxygen release while controlling hydrogen peroxide ***,the fabrication of oxygenating and cytocompatible colloidal gels is achieved using an aqueous two-phase *** study thoroughly evaluates the fundamental characteristics of the resulting bioink,including its rheological behaviors,printability,shape fidelity,mechanical properties,and oxygen release ***,this study demonstrates the macroscopic scalability and cytocompatibility of printed constructs produced via cell-laden oxygenating colloidal *** showcasing the effectiveness of extrusion-based bioprinting,this study underscores how it can be used to fabricate biomimetic tissues,indicating its potential for new *** findings presented here advance the bioprinting field by achieving scalability with both high cell viability and the possibility of mimicking specifically oxygenated *** work thereby offers a promising avenue for the development of functional tissues with e

关键词： 3D bioprinting Bioink Colloidal gels Extrusion printing Oxygen-generating microparticle

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Multi-Compartment SCFA Quantification in Human

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American journal of Analytical Chemistry 2024年第6期15卷 177-200页

作者： jérémy Monteiro Antoine Lefèvre Diane Dufour-Rainfray Adeline Oury Gabrielle Chicheri Laurent Galineau Hélène Blasco Lydie Nadal-Desbarats patrick Emond Imaging Brain & Neuropsychiatry iBraiN U1253 Universit de Tours INSERM MetaboHub Tours Tours France CHRU de Tours Service de Mdecine Nuclaire in Vitro Tours France Service de Biochimie et Biologie Molculaire CHRU Tours France

Short-chain fatty acids (SCFA) play an important role in human biochemistry. They originate primarily from the digestive system through carbohydrates microbial fermentation. Most SCFA produced in the colon are absorbed by the intestinal wall and enter the bloodstream to be distributed throughout the body for multiple purposes. At the intestinal level, SCFA play a role in controlling fat storage and fatty acid metabolism. The effects of these beneficial compounds therefore concern overall health. They facilitate energy expenditure and are valuable allies in the fight against obesity and diabetes. SCFA are also involved in the regulation of the levels of several neurotransmitters such as GABA (γ-aminobutyric acid), glutamate, serotonin, dopamine, and norepinephrine. Their role is also highlighted in many inflammatory and neurodegenerative diseases such as Alzheimer’s disease (AD) or Parkinson’s disease (PD). To have a realistic picture of the distribution of SCFA in different biological compartments of the human body, we propose to study SCFA simultaneously in five human biological samples: feces, saliva, serum, cerebrospinal fluid (CSF), and urine, as well as in Dried Blood Spot (DBS). To evaluate their concentration and repeatability, we used 10 aliquots from pooled samples, analyzed by 3-nitrophenylhydrazine (3-NPH) derivation and liquid chromatography coupled with high sensitivity mass spectrometry (LC-QqQ-MS). We also evaluated the SCFA assay on Dried Blood Spot (DBS). In this work, we adapted the pre-analytical parts for each sample to be able to use a common calibration curve, thus facilitating multi-assay quantification studies and so being less time-consuming. Moreover, we proposed new daughter ions from the same neutral loss (43 Da) to quantify SCFAs, thus improving the sensitivity. In conclusion, our methodology, based on a unique calibration curve for all samples for each SCFA, is well-suited to quantified them in a clinical context.

关键词： LC-MS 3-Nitrophenylhydrazine Short-Chain Fatty Acids Human Biological Samples Quantification

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