*** stress induced in retinal pigmented epithelium cells by the synergy between polycyclic aromatic hydrocarbons and blue light
作者机构：Axe Médecine RégénératriceCentre de Recherche du CHU de QuébecUniversitéLavalHôpital du Saint-SacrementQuébecQCCanada Centre de Recherche en Organogénèse Expérimentale de l'UniversitéLaval/LOEXUniversitéLavalQuébecQCCanada Département d’Ophtalmologie et ORL-chirurgie cervico-facialeUniversitéLavalQuébecQCCanada
出 版 物：《Annals of Eye Science》 (眼科学年鉴（英文）)
年 卷 期：2018年第1期
页 面：450-450页
学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100401[医学-流行病与卫生统计学] 10[医学]
主 题：Retinal pigment epithelium(RPE) polycyclic aromatic hydrocarbons(PAH) blue light oxidative stress age-related macular degeneration(AMD)
摘 要：Background:Lesion to the retinal pigment epithelium(RPE)is a crucial event in age-related macular degeneration(AMD)*** the pathogenesis of this complex disease is poorly understood,sunlight exposure and smoking are major environmental risk factors associated with ***-energy visible blue light(HEV;400-500 nm)is the most energetic and potentially harmful solar wavelengths reaching adults *** the other hand,RPE cells can be exposed to a large range of pollutants from cigarette smoke,with polycyclic aromatic hydrocarbons(PAH)being among the most *** PAH from cigarette smoke can absorb HEV *** led us hypothesize that in RPE cells,the combination of PAH and HEV could synergize to exacerbate the stress caused by either factor *** thus investigate the combined effect of PAH and HEV light in RPE ***:Confluent RPE immortalized cells(ARPE19)were exposed to nanomolar concentrations of benzo[a]pyrene(BaP)or indeno[1,2,3-cd]pyrene(IcdP).While IcdP efficiently absorbs HEV wavelengths,BaP,the most studied PAH,does not significantly absorb HEV light and was used as a *** or IcdP contaminated ARPE19 were then irradiated with increasing sub-lethal doses of HEV light(150-500 J/cm2)using a setup that mimics the light spectrum normally reaching the ***,apoptosis and reactive oxygen species(ROS)generation were assessed in each ***:In presence of low concentrations of IcdP,sub-lethal amounts of HEV light trigger,in a dose-dependent way,up to 70%of apoptotic cell ***-exposure to IcdP and HEV also leads to a synergistic ROS generation in ARPE19 cells,thus inducing oxidative *** of these effects were observed with *** inhibition of ROS production by specific antioxidants only decreases death by 20%in cells simultaneously exposed to both IcdP and HEV ***:Low concentrations of IcdP synergize with HEV light to induce phototoxicity in ARPE19 *** increased oxidat
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