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Silk-based nerve guidance conduits with macroscopic holes modulate the vascularization of regenerating rat sciatic nerve

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Neural Regeneration Research 2025年第6期20卷 1789-1800页

作者： Carina Hromada patrick Heimel Markus Kerbl lászlóGál Sylvia Nürnberger Barbara Schaedl James Ferguson Nicole Swiadek Xavier Monforte Johannes C.Heinzel Antal Nógrádi Andreas H.Teuschl-Woller David Hercher Department life Science Engineering University of Applied Sciences Technikum WienViennaAustria Austrian Cluster for Tissue Regeneration ViennaAustria ludwig Boltzmann Institute for Traumatology The Research Center in Cooperation with AUVAViennaAustria University Clinic of Dentistry Medical University of ViennaViennaAustria Department of Plastic Reconstructive and Aesthetic SurgeryLandesklinikum Wiener Neustadt2700 Wiener NeustadtAustria Department of Anatomy Histology and EmbryologyAlbert Szent-Györgyi Medical SchoolUniversity of SzegedSzegedHungary Medical University of Vienna Department of Orthopedics and Trauma SurgeryDevision of Trauma SurgeryViennaAustria Department of Hand- PlasticReconstructive and Burn SurgeryBG Unfallklinik TuebingenUniversity of TuebingenTuebingenGermany

Peripheral nerve injuries induce a severe motor and sensory deficit. Since the availability of autologous nerve transplants for nerve repair is very limited, alternative treatment strategies are sought, including the use of tubular nerve guidance conduits(tNGCs). However, the use of tNGCs results in poor functional recovery and central necrosis of the regenerating tissue, which limits their application to short nerve lesion defects(typically shorter than 3 cm). Given the importance of vascularization in nerve regeneration, we hypothesized that enabling the growth of blood vessels from the surrounding tissue into the regenerating nerve within the tNGC would help eliminate necrotic processes and lead to improved regeneration. In this study, we reported the application of macroscopic holes into the tubular walls of silk-based tNGCs and compared the various features of these improved silk^(+) tNGCs with the tubes without holes(silk^(–) tNGCs) and autologous nerve transplants in an 8-mm sciatic nerve defect in rats. Using a combination of micro-computed tomography and histological analyses, we were able to prove that the use of silk^(+) tNGCs induced the growth of blood vessels from the adjacent tissue to the intraluminal neovascular formation. A significantly higher number of blood vessels in the silk^(+) group was found compared with autologous nerve transplants and silk^(–), accompanied by improved axon regeneration at the distal coaptation point compared with the silk^(–) tNGCs at 7 weeks postoperatively. In the 15-mm(critical size) sciatic nerve defect model, we again observed a distinct ingrowth of blood vessels through the tubular walls of silk^(+) tNGCs, but without improved functional recovery at 12 weeks postoperatively. Our data proves that macroporous tNGCs increase the vascular supply of regenerating nerves and facilitate improved axonal regeneration in a short-defect model but not in a critical-size defect model. This study suggests that further optimizatio

关键词： axon regeneration blood vessel functional recovery macroporous nerve lesion peripheral nerve repair sciatic nerve silk-based nerve guidance conduit vascularization

Development of high damage threshold laser-machined apodizers and gain filters for laser applications

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High Power laser Science and Engineering 2016年第3期4卷 113-119页

作者： patrick Rambo Jens Schwarz Mark Kimmel John l.Porter Sandia National laboratories

We have developed high damage threshold filters to modify the spatial profile of a high energy laser beam. The filters are formed by laser ablation of a transmissive window. The ablation sites constitute scattering centers which can be filtered in a subsequent spatial filter. By creating the filters in dielectric materials, we see an increased laser-induced damage threshold from previous filters created using ‘metal on glass' lithography.

关键词： apodization laser beam shaping laser micro-machining spatial filtering

Climate Change and Temperature-related Mortality:Implications for Health-related Climate Policy

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Biomedical and Environmental Sciences 2021年第5期34卷 379-386页

作者： TONG Shi lu Jom Olsen patrick l.Kinney Shanghai Children’s Medical Center Shanghai Jiao Tong University School of MedicineShanghai 200127China School of Public Health Institute of Environment and Population HealthAnhui Medical UniversityHefei 230032AnhuiChina Center for Global Health School of Public HealthNanjing Medical UniversityNanjing 210029JiangsuChina School of Public Health and Social Work Queensland University of TechnologyBrisbane 4059Australia Department of Clinical Epidemiology University of AarhusAarhus 8000Denmark Department of Environmental Health Boston University School of Public HealthBoston MA02215USA

It is widely recognized that climate change is one of the greatest challenges humanity faces in the 21st century[1,2].As the leading authority for the assessment of climate change,the Intergovernmental Panel on Climate Change (IPCC),in its special report on global warming of 1.5℃,has concluded that human activities have caused approximately 1.0℃ of global warming above preindustrial levels,with a likely increase of 1.5℃ between 2030 and 2052[3].Climate-related risks for natural and human systems are projected to increase with global warming of 1.5℃,and increase further with 2℃ or *** risks depend on the magnitude and rate of warming,geographic location,levels of development and vulnerability,and on the choices and implementation of adaptation and mitigation options.

关键词： warming magnitude likely

Effects of la^(3+) and Gd^(3+) on Ca^(2+) influx in rat hepatoma H-35 cells

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Chinese Science Bulletin 1999年第4期44卷 331-335页

作者： WONG patrick C. l. Department of Biochemistry The University of Hong Kong

Effects of la93+) and Gd3+ on Ca2+ influx were investigated in rat hepatoma H-35 cells by measuring the initial rate of45Ca2+ uptake. It was found that the maximum initial rate of Ca2+ uptake was increased six- to ten-fold at low concentrations of la3+ and Gd3+. Kinetic analyses by measuring the initial rate of Ca2+ influx at different external Ca2+ concentrations indicated the existence of two intracellular exchangeable components in the basal Ca2+ system, with low and high affinities for Ca2+,and only one class of Ca2+ binding sites was observed in the la3+- or Gd3+-treated cells. For high affinity, la3+ and Gd3+ increased both kinetic parameters Km and V(max of basal Ca2+ influx. la3+ and Gd3+ compete directly with Ca2+ for Ca2+ binding site for low affinity. The kinetics is competitive.

关键词： la3+ Gd3+ rat hepatoma H-35 cells Ca2+ influx.

lineage specification of early embryos and embryonic stem cells at the dawn of enabling technologies

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National Science Review 2017年第4期4卷 533-542页

作者： Guangdun Peng patrick P.l.Tam Naihe Jing State Key laboratory of Cell Biology CAS Center for Excellence in Molecular Cell Science Shanghai Institute of Biochemistry and Cell Biology Chinese Academy of SciencesUniversity of Chinese Academy of Sciences Embryology Unit Children’s Medical Research Institute School of Medical SciencesSydney Medical School University of Sydney School of life Science and Technology Shanghai Tech University

Establishment of progenitor cell populations and lineage diversity during embryogenesis and the differentiation of pluripotent stem cells is a fascinating and intricate biological process. Conceptually, an understanding of this developmental process provides a framework to integrate stem-cell pluripotency, cell competence and differentiating potential with the activity of extrinsic and intrinsic molecular *** recent advent of enabling technologies of high-resolution transcriptome analysis at the cellular,population and spatial levels proffers the capability of gaining deeper insights into the attributes of the gene regulatory network and molecular signaling in lineage specification and differentiation. In this review, we provide a snapshot of the emerging enabling genomic technologies that contribute to the study of development and stem-cell biology.

关键词： early development cell lineage cell identity scRNA-seq spatial transcriptome

Global changes explain the long-term demographic trend of the Eurasian common lizard (Squamata: lacertidae)

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Current Zoology 2022年第2期68卷 221-228页

作者： Jose l.HORREO patrick S.FITZE Department of Genetics Physiology and MicrobiologyComplutense University of MadridC/Jose Antonio Novais 12Madrid 28040Spain Department of Biodiversity and Evolutionary Biology National Museum of Natural Sciences(CSIC)C/Jose Gutierrez Abascal 2Madrid 28006Spain

The demographic trend of a species depends on the dynamics of its local populations,which can be compromised by local or by global ***,the relevance of local and global phenomena has rarely been investigated ***,we tested whether local phenomena compromised a species'demographic trend using the Eurasian common lizard Zootoca vivipara,the terrestrial reptile exhibiting the widest geographic distribution,as a model *** analyzed the species'ancient demographic trend using genetic data from its 6 allopatric genetic clades and tested whether its demographic trend mainly depended on single clades or on global *** vivipara's effective population size increased since 2.3 million years ago and started to increase steeply and continuously from 0.531 million years *** growth rate exhibited 2 maxima,both occurring during global climatic changes and important vegetation changes on the northern *** population size and growth rate were negatively correlated with global surface temperatures,in line with global parameters driving long-term demographic *** vivipara's ancient demography was neither driven by a single clade,nor by the 2 clades that colonized huge geographic areas after the last *** low importance of local phenomena,suggests that the experimentally demonstrated high sensitivity of this species to short-term ecological changes is a response in order to cope with short-term and local *** suggests that what affected its long-term demographic trend the most,were not these local changes/responses,but rather the important and prolonged global climatic changes and important vegetation changes on the northern hemisphere,including the opening up of the forest by humans.

关键词： ancient demographic trend anthropological activity Bayesian Skyline Plots early humans Eemian interglacial Eurasian common lizard mid-Brunhes event

Cloning and expression of SlC10A4,a putative organic anion transport protein

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World Journal of Gastroenterology 2006年第42期12卷 6797-6805页

作者： patrick l Splinter Konstantinos N lazaridis Paul A Dawson Nicholas F laRusso The Center for Basic Research in Digestive Disease Division of Gastroenterology and Hepatology Mayo Medical School Clinic and Foundation Rochester Minnesota 55905 United States Department of Internal Medicine-Gastroenter-ology Wake Forest University School of Medicine Winston-Salem North Carolina 27157 United States

AIM: To determine if novel bile acid transporters may be expressed in human tissues. METHODS: SlC10A1 (NTCP) was used as a probe to search the NCBI database for homology to previously uncharacterized ESTs. The homology search identified an EST (termed SlC10A4) that shares sequence identity with SlC10A1 and SlC10A2 (ASBT). We performed Northern blot analysis and RT-PCR to determine the tissue distribution of SlC10A4. SlC10A4 was cloned in frame with an epitope tag and overexpressed in CHO cells to determine cellular localization and functional analysis of bile acid uptake. RESUlTS: Northern analysis revealed that SlC10A4 mRNA is ubiquitously expressed in human tissues with the highest levels of mRNA expression in brain, placenta, and liver. In SlC10A4-transfected CHO cells, immunoblotting analysis and immunofluorescence staining demonstrated a 49-kDa protein that is expressed at the plasma membrane and intracellular compartments. Functional analysis of SlC10A4 showed no significant taurocholate uptake in the presence of sodium when compared to untransfected CHO cells. CONClUSION: To date, we have shown that this protein has no capacity to transport taurocholate relative to SlC10A1; however, given its ubiquitous tissue distribution, it may play a more active role in transporting other endogenous organic anions.

关键词： SlC10A4 Bile acid transport Biliaryepithelium Taurocholate Plasma membrane

Integration of Computational Analysis and Spatial Transcriptomics in Single-cell Studies

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Genomics, Proteomics & Bioinformatics 2023年第1期21卷 13-23页

作者： Ran Wang Guangdun Peng patrick P.l.Tam Naihe Jing State Key laboratory of Cell Biology CAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai 200031China CAS Key laboratory of Regenerative Biology Guangdong Provincial Key Laboratory of Stem Cell and Regenerative MedicineGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijing 100101China Embryology Research Unit Children’s Medical Research InstituteUniversity of SydneySydneyNSW 2145Australia School of Medical Sciences Faculty of Medicine and HealthUniversity of SydneySydneyNSW 2145Australia Guangzhou laboratory Guangzhou 510005China

Recent advances of single-cell transcriptomics technologies and allied computational methodologies have revolutionized molecular cell ***,pioneering explorations in spatial transcriptomics have opened up avenues to address fundamental biological questions in health and ***,we review the technical attributes of single-cell RNA sequencing and spatial transcriptomics,and the core concepts of computational data *** further highlight the challenges in the application of data integration methodologies and the interpretation of the biological context of the findings.

关键词： scRNA-seq Computational methodology Spatial transcriptome Data integration Mathematical model

Mixers with Microstructured Foils for Chemical Production Purposes

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化工进展 2005年第5期24卷 570-576页

作者： Bernd Werner Volker Hessel patrick lb Institut für Mikrotechnik Mainz GmbH Carl-Zeiss-Straβe 18-20D-55129 Institut für Mikrotechnik Mainz GmbHCarl-Zeiss-Straβe 18-20D-55129 MainzGermanyInstitut für Mikrotechnik Mainz GmbHCarl-Zeiss-Straβe 18-20D-55129 MainzGermany

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关键词：化工行业 IMM 科研实力微观结构混频器