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Characterization of Cnidoscolus quercifolius Pohl bark root extract and evaluation of cytotoxic effect on human tumor cell lines

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Asian Pacific Journal of Tropical Biomedicine 2018年第7期8卷 345-351页

作者： Paulo Fernando Machado Paredes Selene Maia de Morais Fernando Cesar rodrigues Brito Luiz Francisco Wemmenson Goncalves Moura patricia de Araujo rodrigues Stephen Rathinaraj Benjamin Francisco Ernani Alves Magalhaes Eridan Orlando Pereira Tramontina Florean Maria Izabel Florindo Guedes Northeast Biotechnology Network Graduate Program of Biotechnology State University of Ceara Itaperi campus Department of Chemistry State University of Ceara Itaperi campus Laboratory of Bioprospecting of Natural Products and Biotechnology State University of CearaCECITEC

Objective: To evaluate the chemical components of active extract from Cnidoscolus quercifolius root bark and its cytotoxic potential against several tumor strains. Methods: The high-performance liquid chromatography with diode-array detection and ~1H and ^(13)C nuclear magnetic resonance spectroscopy of the extract were used to distinguish the existence of possible functional groups in the root bark extract. The in vitro cytotoxic activity of methanol extract on human colon cancer cell lines was evaluated using OVCAR-8, SF-295, HCT-116, HL-60 strains and the samples were assessed by 3-(4,5-dimethylthiazol2-yl)-2,5-diphenyltetrazolium bromide method. Results: The analysis of nuclear magnetic spectra of the active chloroform fraction revealed the presence of absorptions bands correspondent to a mixture of favelines such as neofavelanone, deoxofaveline or methyl-faveline, which structures were confirmed by ultraviolet spectra upon high-performance liquid chromatography with diode-array detection analysis. The active fraction showed cytotoxic effects in the tested strains, HCT-116, SF-295, OVCAR-8 and HL-60 cells with IC_(50) of 72 hours ranging from 4.95 to 15.23 μg/mL. Conclusions: The results suggest that the substances present in faveleira(Cnidoscolus quercifolius) root bark extract have a cytotoxic potential against several tumor lines, showing a broader antitumour potential, and in addition no adverse to healthy cells. Therefore, the root bark extract of Cnidoscolus quercifolius has a possibility of use for anticarcinogenic therapies.

关键词： Cnidoscolus quercifolius Pohl. methyl-faveline Deoxofaveline Neofavelanone Faveline Cytotoxicity

A Closed-Form Solution of a Kinetic Integral Equation for Rarefied Gas Flow in a Cylindrical Duct

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Applied Mathematics 2014年第10期5卷 1516-1527页

作者： Carmo Henrique Kamphorst patricia rodrigues Liliane Basso Barichello Departamento de Ciencias Exatas e da Terra Universidade Regional Integrada do Alto Uruguai e das Missoes(URI)Frederico WestphalenBrazil Departamento de Ciencias Agronomicas e Ambientais Universidade Federal de Santa Maria(UFSM)Frederico WestphalenBrazil Instituto de Matematica Universidade Federal do Rio Grande do Sul(UFRGS)Porto AlegreBrazil

A spectral method based on Hermite cubic splines expansions combined with a collocation scheme is used to develop a solution for the vector form integral S-model kinetic equation describing rarefied gas flows in cylindrical geometry. Some manipulations are made to facilitate the computational treatment of the singularities inherent to the kernel. Numerical results for the simulation of flows generated by pressure and thermal gradients, Poiseuille and thermal-creep problems, are presented.

关键词： Rarefied Gas Dynamics Integral Equation S-Model Collocation Schemes

Does Locomotive Syndrome, Associated with Sarcopenia or otherwise, Influence Quality of Life in Individuals Aged over 80 years? Third Wave of the LOCOMOV Project

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Journal of Geriatric Medicine 2021年第2期3卷 17-26页

作者： patricia de Castro rodrigues Julia Carvalho Galiano Virginia Fernandes Moça Trevizani Fania Cristina Santos Discipline of Geriatrics and Gerontology(DIGG) Federal University of São Paulo(UNIFESP)São PauloBrazil Department of Evidence-Based Medicine Brazilian Cochrane CentreFederal University of São Paulo(UNIFESP)Sao PauloBrazil

Introduction:Locomotion is a determinant of intrinsic capacity of older people and can be limited by dysfunction in locomotory organs,characterizing Locomotive Syndrome(LoS).Knowledge on locomotive problems and sarcopenia,and their interface with quality of life,in the oldest old in the literature is ***:To evaluate the correlation between LoS and sarcopenia and their influence on quality of life in oldest ***:A cross-sectional study of an observational,descriptive and analytical epidemiological survey in independent older adults aged 80 and over from São Paulo,Brazil and who participated in the third wave of the LOCOMOV Project,was carried *** data,comorbidities,functioning in activities of daily living,physical functioning,quality of life,and presence of sarcopenia and LoS were *** statistical analyses included the Test-for-Comparing-Two-Proportions,Pearson's Correlation Coefficient,the chi-Square test and Student´s ***:Thirty oldest old with a mean age of 89.1 years were *** prevalence of LoS was high(53.3%)and correlated significantly with chronic pain(p-value 0.024),worse performance on the SPPB and Gait speed(p-value<0.001).Sarcopenia was not correlated with LoS,but worse quality of life on the physical domain was significantly associated with LoS(p-value<0.001)regardless of the presence of ***:LoS was highly prevalent among the oldest old studied and negatively impacted their quality of life,regardless of the presence of sarcopenia.

关键词： Older adults Longevity Locomotive syndrome Sarcopenia Functioning Physical tests Quality of life

Risk factors for suicidal behaviour in late-life depression:A systematic review

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World Journal of Psychiatry 2022年第1期12卷 187-203页

作者： Veronica Fernandez-rodrigues Yolanda Sanchez-Carro Luisa Natalia Lagunas Laura Alejandra Rico-Uribe Andres Pemau patricia Diaz-Carracedo Marina Diaz-Marsa Gonzalo Hervas Alejandro de la Torre-Luque Department of Psychology Universidad Complutense de MadridMadrid 28223Spain Department of Psychiatry Universidad Autonoma de MadridMadrid 28046Spain Centre for Biomedical Research in Mental Health(CIBERSAM) Madrid 28029Spain Department of Legal Medicine Psychiatry and PathologyUniversidad Complutense de MadridMadrid 28046Spain of Psychology La Rioja International UniversityLogrono 26006Spain Institute of Psychiatry and Mental Health San Carlos Clinical HospitalMadrid 28040Spain

BACKGROUND Suicide is a leading cause of preventable death worldwide,with its peak of maximum incidence in later *** often puts an individual at higher risk for suicidal *** turn,depression deserves particular interest in old age due to its high prevalence and dramatic impact on health and *** To gather integrated evidence on the potential risk factors for suicide behaviour development in depressive older adults,and to examine the effects of depression treatment to tackle suicide behaviour in this *** A systematic review of empirical studies,published from 2000 onwards,was *** behaviour was addressed considering its varying forms(i.e.,wish to die,ideation,attempt,and completed suicide).RESULTS Thirty-five papers were selected for review,comprising both clinical and epidemiological *** of studies focused on suicidal ideation(60%).The studies consistently pointed out that the risk was related to depressive episode severity,psychiatric comorbidity(anxiety or substance use disorders),poorer health status,and loss of *** social support and loneliness were also associated with suicide behaviour in depressive older ***,the intervention studies showed that suicidal behaviour was a robust predictor of depression treatment *** in suicidal ideation were moderated by reductions in risk factors for suicide *** To sum up,common and age-specific risk factors seem to be involved in suicide development in depressive older adults.A major effort should be made to tackle this serious public health concern so as to promote older people to age healthily and well.

关键词： Late-life depression Suicide behaviour Disability Chronic disease Loneliness

Dual-functional porous and cisplatin-loaded polymethylmethacrylate cement for reconstruction of load-bearing bone defect kills bone tumor cells

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Bioactive Materials 2022年第9期7卷 120-130页

作者： Zhule Wang Liebert Parreiras Nogueira Håvard Jostein Haugen Ingrid CM.Van Der Geest patricia Caetano de Almeida rodrigues Dennis Janssen Thom Bitter Jeroen J.J.P.van den Beucken Sander CG.Leeuwenburgh Radboud University Medical Center Department of Dentistry-Regenerative BiomaterialsRadboud Institute for Molecular Life SciencesNijmegenthe Netherlands University of Oslo Department of BiomaterialsInstitute of Clinical DentistryFaculty of DentistryOsloNorway Radboud University Medical Center Department of OrthopedicsRadboud Institute for Health SciencesNijmegenthe Netherlands

Malignant bone tumors are usually treated by resection of tumor tissue followed by filling of the bone defect with bone graft ***(PMMA)cement is the most commonly used bone substitute in clinical orthopedics in view of its ***,the dense nature of PMMA renders this biomaterial unsuitable for local delivery of chemotherapeutic drugs to limit the recurrence of bone ***,we introduce porosity into PMMA cement by adding carboxymethylcellulose(CMC)to facilitate such local delivery of chemotherapeutic drugs,while retaining sufficient mechanical properties for bone reconstruction in load-bearing *** results show that the mechanical strength of PMMA-based cements gradually decreases with increasing CMC *** incorporation of≥3%CMC,the PMMA-based cements released up to 18%of the loaded cisplatin,in contrast to cements containing lower amounts of CMC which only released less than 2%of the cisplatin over 28 *** release of cisplatin efficiently killed osteosarcoma cells in vitro and the fraction of dead cells increased to 91.3%at day 7,which confirms the retained chemotherapeutic activity of released cisplatin from these PMMA-based ***,tibias filled with PMMA-based cements containing up to 3%of CMC exhibit comparable compressive strengths as compared to intact *** conclusion,we demonstrate that PMMA cements can be rendered therapeutically active by introducing porosity using CMC to allow for release of cisplatin without compromising mechanical properties beyond critical *** such,these data suggest that our dual-functional PMMA-based cements represent a viable treatment option for filling bone defects after bone tumor resection in load-bearing sites.

关键词： Porous polymethylmethacrylate cement Bone tumor treatment Local drug delivery Chemotherapeutic drug Ex vivo biomechanical assessment

Effects of perinatal protein deprivation and recovery on esophageal myenteric plexus

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World Journal of Gastroenterology 2010年第5期16卷 563-570页

作者： Flavio M Greggio Ricardo BV Fontes Laura B Maifrino patricia Castelucci Romeu rodrigues de Souza Edson A Liberti Autonomic Nervous System Laboratory (VQM) Department of Anatomy Instituto de Ciencias Biomedicas da Universidade de Sao Paulo 2415 Av Prof Lineu Prestes 05508-900 So Paulo Brazil

AIM:To evaluate effects of preand postnatal protein deprivation and postnatal recovery on the myenteric plexus of the rat esophagus. METHODS: Three groups of young Wistar rats (aged 42 d) were studied: normalfed (N42), proteindeprived (D42), and proteinrecovered (R42). The myenteric neurons of their esophagi were evaluated by histochemical reactions for nicotinamide adenine dinucleotide (NADH), nitrergic neurons (NADPH)diaphorase and acetylcholinesterase (AChE), immunohistochemical reaction for vasoactive intestinal polypeptide (VIP), and ultrastructural analysis by transmission electron ***: The cytoplasms of large and medium neurons from the N42 and R42 groups were intensely reactive for NADH. Only a few large neurons from the D42 group exhibited this aspect. NADPH detected in the D42 group exhibited low reactivity. The AChE reactivity was diffuse in neurons from the D42 and R42 groups. The density of large and small varicosities detected by immunohistochemical staining of VIP was low in ganglia from the D42 group. In many neurons from the D42 group, the double membrane of the nuclear envelope and the perinuclear cisterna were not detectable. NADH and NADPH histochemistry revealed no group differences in the prof ile of nerve cell perikarya (ranging from 200 to 400 μm2).CONCLUSION: Protein deprivation causes a delay in neuronal maturation but postnatal recovery can almost completely restore the normal morphology of myenteric neurons.

关键词： Esophagus Enteric nervous system Myenteric plexus Proteins Light microscopy Transmission electron microscopy

Ground peach pits: alternative substrate component for seedling production

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Journal of Forestry Research 2019年第5期30卷 1779-1791页

作者： patricia Mieth Maristela Machado Araujo Maria Helena Fermino Suelen Carpenedo Aimi Daniele rodrigues Gomes Juliana de Marques Vilella Department of Forest Sciences Federal University of Santa MariaSanta MariaBrazil FEPAGRO–Agricultural Research Foundation of the State of Rio Grande do Sul Porto AlegreBrazil Federal University of Rio Grande do Sul Porto AlegreBrazil

The exploitation of agro-industrial wastes is a strategy to add value to seedling production and reduce environmental *** production chain for processed peaches generates,approximately,21,000 tons of fruit worldwide,and the pits are used mainly to generate *** objective of the study was to investigate the use of different proportions of ground peach pits(GPP)as a substrate component for seedling growth of Eucalyptus dunnii in the nursery and *** proportions of GPP mixed with either commercial substrate(CS)or with brown peat(BP)were *** physical and chemical properties of the substrates,the morphological and physiological attributes of the seedlings and their correlation with substrate properties were *** GPP increased the density,aeration space and pH and reduced total porosity,liquid retention and electric conductivity of the *** use of this component resulted in hormesis,a biphasic dose–response model in which low levels of exposure to this component stimulated biological performance of plants and high levels of exposure inhibited *** improved seedling growth in the nursery and adequate post-planting development of ***,GPP with CS is recommended at 20%of the total substrate and with BP between 15 and 30%.

关键词： Organic waste Prunus persica Eucalyptus dunnii Tree species Morphological attributes

Carbon and nitrogen mineralization in soil of leguminous trees in a degraded pasture in northern Rio de Janeiro, Brazil

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Journal of Forestry Research 2016年第1期27卷 91-99页

作者： Danielle A. D. Nunes Emanuela F. Gama-rodrigues patricia A. B. Barreto[ Antonio C. Gama-Rodrigue Paulo H. M. Monroe Chemistry School Rio de Janeiro Federal UniversityRio de Janeiro RJ 21941-909 Brazil Soil Laboratory North Fluminense State UniversityCampos dos Goytacazes RJ 28013-602 Brazil Department of Soil and Agricultural Engineering State University of Southwest Bahia Vitoda da ConquistaBA 45031-300 Brazil

Use of legume trees can improve soil quality in degraded pastures. The aim of this study was to charac- terize C and N mineralization kinetics and estimate the potentially mineralizable C and N in soil under Mimosa caesalpiniifolia Benth. and Acacia auriculiformis A. Cunn. ex Benth. secondary forest and pasture in red-yellow latosols in southeast Brazil. We conducted a laboratory aerobic incubation experiment using a completely ran- domized design of four replicates and four types of plant cover using a modified version of the Stanford and Smith technique （1972） to study C and N mineralization potential. Potentially mineralizable N （No） ranged from 135 to 170 mg kg-1. The predominant form of mineral N for all types of plant cover was N-NO3-. M. caesalpiniifolia was the only species that had a positive influence on N min- eralization. Neither of the legumes influenced C mineral- ization in pasture or secondary forest. The model of N mineralization corresponded to a sigmoidal curve while C mineralization corresponded to an exponential curve, revealing that the N and C mineralization processes were distinct. N mineralized by M. caesalpiniifolia （216 kg ofN ha-1） was adequate to meet the N requirement for a livestock-forest system.

关键词： Carbon dioxide Forest soil Mineralized N Organic matter Soil microorganisms

Apixaban, an orally available anticoagulant, inhibits SARS-CoV-2 replication and its major protease in a non-competitive way

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Journal of Molecular Cell Biology 2022年第6期14卷 68-71页

作者： Otávio Augusto Chaves Carolina Q.Sacramento Natalia Fintelman-rodrigues Jairo Ramos Temerozo Filipe Pereira-Dutra Daniella M.Mizurini Robson Q.Monteiro Leonardo Vazquez patricia T.Bozza Hugo Caire Castro-Faria-Neto Thiago Moreno L.Souza Laboratory of Immunopharmacology Oswaldo Cruz Institute(IOC)Oswaldo Cruz Foundation(Fiocruz)Rio de JaneiroRJ21040-900Brazil National Institute for Science and Technology on Innovation on Neglected Diseases(INCT/IDN) Center for Technological Development in Health(CDTS)Oswaldo Cruz Foundation(Fiocruz)Rio de JaneiroRJ21040-900Brazil National Institute for Science and Technology on Neuroimmunomodulation(INCT/NIM) Oswaldo Cruz Institute(IOC)Oswaldo Cruz Foundation(Fiocruz)Rio de JaneiroRJ21040-900Brazil Laboratory on Thymus Research Oswaldo Cruz Institute(IOC)Oswaldo Cruz Foundation(Fiocruz)Rio de JaneiroRJ21040-900Brazil Institute of Medical Biochemistry Leopoldo de Meis Federal University of Rio de Janeiro(UFRJ)Rio de JaneiroRJ21941-590Brazil

Dear Editor,The severe coronavirus disease 2019(COVID-19)is associated with ***,such as low-molecular-weight heparin,warfarin,thrombin inhibitors,and factor Xa(FXa)inhibitors,are thus recommended by the American Society of Hematology and National Institutes of Health for COVID-19 patients(Wenzler et al.,2020;Adam et al.,2021).Clinical trials with anticoagulants have shown the increased survival of critically ill COVID-19 patients under non-invasive and invasive ventilatory assistance(Wenzler et al.,2020;Adam et al.,2021),along with decreased consumption of platelets and clotting factors and a reduced risk of hemorrhage(Adam et al.,2021).Among the anti-clotting agents,early use of orally available FXa and thrombin inhibitors(Chowdhury et al.,2020;Rentsch et al.,2021)prevented high levels of D-dimer,which is the final product from the clotting/fibrinolysis cascade and is directly implicated with severe COVID-19(Rentsch et al.,2021).

关键词： al. Clinical heparin