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Decreased morphogenetic potential in peach palm stem-like cells in long-term in vitro conditions

Journal of Forestry Research 2020年第2期31卷 485-495页

作者： Erika Mendes Graner Gilvano Ebling Brondani Cristina vieira de Almeida Katherine Derlene Batagin-Piotto Marcilio de Almeida Luiz de Queiroz College of Agriculture University of Sao PauloPiracicabaSPBrazil Department of Forest Sciences Campus UniversitarioFederal University of LavrasLavrasMGBrazil In Vitro Palm(Consulting Study and Biological Development Ltda.)PiracicabaSPBrazil

Peach palm(Bactris gasipaes Kunth)has been micropropagated from pre-procambial cells that provide stem-like cell niches,(i.e.,pre-procambial cells),multipotent,pluripotent and totipotent for direct vascularization,adventitious buds and somatic embryogenesis,*** direct induction of adventitious buds and somatic embryogenesis reduces the frequency of mutations when compared to indirect ***-term in vitro cultivation of perennial species such as peach palm cause the clones to age and deteriorate;however,the consequences for morphogenesis potential are not fully *** morphogenic potential of peach palm clones established and in vitro cultivated for 8 years(regeneration of adventitious buds without callus formation)was investigated in leaves,roots and stem bases using histological and histochemical *** from long-term cultures(8-years-old)was compared to data from short-term cultures(1-year-old).Morphogenic pathways monitoring for direct induction of somatic embryos and adventitious buds revealed a strong morphogenic reduction potential in the pre-procambial cells,parenchyma cells in the proximal region of stem bases,and external cells of leaf *** cells of shoot apical meristems and pre-procambial cells commit cell reprogramming to the undifferentiated state and subsequent acquisition of cellular *** results are applicable in the micropropagation of peach palm,with consideration to obtaining clones and their long-term in vitro culture.

关键词： Micropropagation Multipotency Pluripotency Totipotency Pre-procambial cells Peach palm Bactris gasipaes

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Impact of technology use in type 2 diabetes distress:A systematic review

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World Journal of Diabetes 2020年第10期11卷 459-467页

作者： patricia vieira Renata Kobayasi Filomena Pereira Isabella Martins Zaia Sandra Umeda Sasaki Department of Health Iron SaúdeAlcabideche 2649-517Portugal Department of Internal Medicine University of Sao PauloSao Paulo 05410-020Brazil School of Psychology and Science of Education University of PortoPorto 4200-465Portugal Medical School UNICID Sao Paulo 03071-000Brazil

BACKGROUND Diabetes distress is an important factor in treatment outcomes and results in poor behavioral and biological *** has been used in management programs of diabetes to improve communication between patients and health care providers and to promote education about the disease and its psychological aspects,which can impact the self-efficacy of the ***,the true impact of technological approaches on the management of type 2 diabetes distress remains *** To investigate the effectiveness of technology interventions on the management of type 2 diabetes *** Studies published from 2014 to 2019 were searched in five databases:MEDLINE,PubMed,Library and Information Science Source,Academic Search Ultimate and *** Boolean logic search terms were:(1)T2Diabetes;(2)diabetes distress;and(3)technology OR mobile OR phone OR application OR *** also systematically searched the reference lists of the included studies and relevant *** controlled trials with technology interventions,type 2 diabetes patients and diabetes distress as the outcome were *** Preferred Reporting Items for Systematic Reviews and Meta-Analyses statement was *** Of the 88 studies selected,nine full articles met the inclusion criteria and were subjected to final careful *** the JADAD scale,one article was classified as having poor quality and eight as having good *** out of nine articles showed that technology interventions had a positive impact on diabetes distress scale scores when compared with the initial *** the six articles,five showed a greater reduction in the diabetes distress scores from control ***-based interventions had good results when users received personalized feedback and routine caregiver support and *** Technology interventions can contribute positively to the management of type 2 diabetes distress,especially with a tailored appro

关键词： Technology Type 2 diabetes Diabetes distress Healthcare Systematic review

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Morphological and molecular diversity of arbuscular mycorrhizal fungi in revegetated iron-mining site has the same magnitude of adjacent pristine ecosystems

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Journal of Environmental Sciences 2018年第5期30卷 330-343页

作者： Caroline Krug vieira Matheus Nicoletti Marascalchi Arthur Vinicius Rodrigues Rafael Dutra de Armas Sidney Luiz Stürmer Universidade Regional de Blumenau(FURB) Programa de Pos-Graduacao em Engenharia Ambiental89030-903 BlumenauSCBrazil Universidade Regional de Blumenau(FURB) Departamento de Ciencias Naturais(DCN)89030-903 BlumenauSCBrazil Uniuersidade Regional de Blumenau(FURB) Programaa de Pos-Graduacao em Engenharia Florestal89030-903 BlumenauSCBrazil Centro Universitario-Catolica de Santa Catarina 89203-005 JoinvilleSCBrazil

Arbuscular mycorrhizal fungi（AMF） are important during revegetation of mining sites, but few studies compared AMF community in revegetated sites with pristine adjacent ecosystems. The aim of this study was to assess AMF species richness in a revegetated iron-mining site and adjacent ecosystems and to relate AMF occurrence to soil chemical parameters. Soil samples were collected in dry and rainy seasons in a revegetated iron-mining site（RA） and compared with pristine ecosystems of forest（FL）, canga（NG）,and Cerrado（CE）. AMF species were identified by spore morphology from field and trap cultures and by LSU r DNA sequencing using Illumina. A total of 62 AMF species were recovered, pertaining to 18 genera and nine families of Glomeromycota. The largest number of species and families were detected in RA, and Acaulospora mellea and Glomus sp1 were the most frequent species. Species belonging to Glomeraceae and Acaulosporaceae accounted for 42%–48% of total species richness. Total number of spores and mycorrhizal inoculum potential tended to be higher in the dry than in the rainy season, except in RA. Sequences of uncultured Glomerales were dominant in all sites and seasons and five species were detected exclusively by DNA-based identification. Redundancy analysis evidenced soil p H,organic matter, aluminum, and iron as main factors influencing AMF presence. In conclusion, revegetation of the iron-mining site seems to be effective in maintaining a diverse AMF community and different approaches are complementary to reveal AMF species, despite the larger number of species being identified by traditional identification of field spores.

关键词： Community structure Glomeromycota Illumina LSU rDNA Spore morphology Taxonomic distinctness index

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Prevalence of sarcopenia using different methods in patients with non-alcoholic fatty liver disease

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World Journal of Hepatology 2022年第8期14卷 1643-1651页

作者： Naiade Silveira Almeida Raquel Rocha Claudineia Almeida de Souza Ana Carolina Sirelli da Cruz Bruna dos Reis Ribeiro Luiza Valois vieira Carla Daltro Rafael Silva Manoel Sarno Helma Pinchemel Cotrim Programa de Pós-Graduação em Medicina e Saúde Universidade Federal da BahiaSalvador 40110-060BABrazil Departamento de Ciência da Nutrição Escola de NutriçãoUniversidade Federal da BahiaSalvador 40110-060Brazil Caliper Clínica e Escola de Imagem Salvador 41810-012Brazil

BACKGROUND Sarcopenia is a clinical condition associated with several liver diseases and it includes non-alcoholic fatty liver disease(NAFLD)in its broad spectrum as steatosis,steatohepatitis and ***,the criteria to define sarcopenia are diverse,and even those established in consensus have been discussed regarding their performance in making an accurate *** To evaluate the prevalence of sarcopenia,using different methods,in patients with NAFLD,and its association with clinical-anthropometric *** This was an observational study of outpatients with *** was defined by the European Working Group Consensus on Sarcopenia in Older People of 2010(EWGSOP1)and 2018(EWGSOP2).The skeletal muscle index was used to estimate muscle mass,handgrip strength was assessed using the dynamometer and physical performance by walking a distance of four meters at usual walking *** non-invasive fibrosis scores,fibrosis-4(FIB-4)index and Aspartate aminotransferase to platelet ratio index(APRI),were used to assess the absence and presence of *** Fifty-seven individuals with NAFLD were evaluated,the mean age(SD)was 52.7(11.3)years and 75.4%were *** assessed by FIB-4 and APRI was observed in 3.7%and 16.6%of patients with NAFLD,*** diagnosis of sarcopenia was identified only by EWGSOP1 in 3.5%of NAFLD patients,and the prevalence of probable/pre-sarcopenia was higher using the EWGSOP2 consensus at 26.3%,when compared to 1.8%with *** defined by EWGSOP1,was associated with grade I steatosis,but without overweight(Pciation between sarcopenia and fibrosis was not observed(P>0.05).EWGSOP2 showed a greater number of patients with probable sarcopenia,and who were overweight(12(80.0%)),with a higher degree of steatosis[11(73.3%)and presence of fibrosis(1(6.7%),FIB-4 and 3(20.0%),APRI]compared to EWGSOP1[1(100%),0(0.0%),0(0.0%),FIB-4 and 0(0.0%),APRI,respectively].CONCLUSION The presen

关键词： Non-alcoholic fatty liver disease Sarcopenia Muscle strength Physical performance Liver fibrosis

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The Power of Inbreeding： NGS-Based GWAS of Rice Reveals Convergent Evolution during Rice Domestication

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Molecular Plant 2016年第7期9卷 975-985页

作者： Hongru Wang Xun Xu Filipe Garrett vieira Yunhua Xiao Zhikang Li Jun Wang Rasmus Nielsen Chengcai Chu State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing) Institute of Genetics and Developmental Biology Chinese Academyof Sciences Beijing 100101 China Cotlege of Life Sciences University of Chinese Academy of Sciences Beijing 100101 China BGI-Shenzhen Shenzhen 518083 China Centre for GeoGenetics University of Copenhagen 1350 Copenhagen Denmark Institute of Crop Sciences/National Key Facilities for Crop Gene Resources and Genetic Improvement Chinese Academy of Agricultural Sciences 12 SouthZhong-Guan-Cun Street Beijing 100081 China Department of Integrative Biology University of California Berkeley CA 94720 USA These authors contributed equally to this article.

Low-coverage whole-genome sequencing is an effective strategy for genome-wide association studies in hu- mans, due to the availability of large reference panels for genotype imputation. However, it is unclear whether this strategy can be utilized in other species without reference panels. Using simulations, we show that this approach is even more relevant in inbred species such as rice （Oryza sativa L.）, which are effectively haploid, allowing easy haplotype construction and imputation-based genotype calling, even without the availability of large reference panels. We sequenced 203 rice varieties with well-characterized phenotypes from the United States Department of Agriculture Rice Mini-Core Collection at an average depth of 1.5~ and used the data for mapping three traits. For the first two traits, amylose content and seed length, our approach leads to direct identification of the previously identified causal SN Ps in the major-effect loci. For the third trait, pericarp color, an important trait underwent selection during domestication, we identified a new major-effect locus. Although known loci can explain color variation in the varieties of two main subspecies of Asian domesticated rice,japonica and indica, the new locus identified is unique to another domesticated rice subgroup, aus, and together with existing loci, can fully explain the major variation in pericarp color in aus. Our discovery of a unique genetic basis of white pericarp in aus provides an example of convergent evolution during rice domestication and suggests that aus may have a domestication history independent of japonica and indica.

关键词： inbreeding GWAS rice pericarp color

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Postoperative pain due to an occult spinal infection:A case report

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World Journal of Clinical Cases 2021年第15期9卷 3637-3643页

作者： Michiel Frederik Vande Kerckhove Vincent Fiere Thais Dutra vieira Sami Bahroun Marc Szadkowski Henrid'Astorg Centre Orthopédique Santy Hopital PrivéJean MermozRamsay-Générale de SantéLyonFrance

BACKGROUND A high degree of vigilance is warranted for a spinal infection,particularly in a patient who has undergone an invasive procedure such as a spinal *** average delay in diagnosing a spinal infection is 2-4 *** our patient,the diagnosis of a spinal infection was delayed by 1.5 *** SUMMARY A 60-year-old male patient with a 1-year history of right-sided lumbar radicular pain failed conservative *** weeks to prior to surgery he received a spinal injection,which was followed by increasing lumbar radicular pain,weight loss and *** went unnoticed and surgery took place with right-sided L4-L5 combined microdiscectomy and foraminotomy via a posterior *** day after surgery,the patient developed left-sided lumbar radicular *** cultures grew Staphylococcus aureus(***).Magnetic resonance imaging showed inflammatory aberrations,revealing septic arthritis of the left-sided L4/L5 facet joint as the probable *** surgery took place and *** was isolated from bacteriological *** patient received postoperative antibiotic treatment,which completely eradicated the *** The development of postoperative lower back pain and/or lumbar radicular pain can be a sign of a spinal infection.A thorough clinical and laboratory work-up is essential in the preoperative evaluation of patients with spinal pain.

关键词： Arthritis Septic Lumbar region Disc herniated Spinal injections Case report

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Natural Mg silicates with different structures and morphologies: Reaction with K to produce K2MgSiO4 catalyst for biodiesel production

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International Journal of Minerals,Metallurgy and Materials 2020年第1期27卷 46-54页

作者： Fabiane Carvalho Ballotin Mayra Nascimento Sara Silveira vieira Alexandre Carvalho Bertoli Ottávio Carmignano Ana Paula de Carvalho Teixeira Rochel Montero Lago Departamento de Química Universidade Federal de Minas GeraisBelo HorizonteBrasil Doutorado Inovação/Mineradora Pedras Congonhas Universidade Federal de Minas Gerais31270-901 Belo Horizonte-MGBrazil

In this work, different magnesium silicate mineral samples based on antigorite, lizardite, chrysotile(which have the same general formula Mg3Si2O5(OH)4), and talc(Mg3Si4O10(OH)2) were reacted with KOH to prepare catalysts for biodiesel production. Simple impregnation with 20 wt% K and treatment at 700–900°C led to a solid-state reaction to mainly form the K2MgSiO4 phase in all samples. These results indicate that the K ion can diffuse into the different Mg silicate structures and textures, likely through intercalation in the interlayer space of the different mineral samples followed by dehydroxylation and K2MgSiO4 formation. All the materials showed catalytic activity for the transesterification of soybean oil(1:6 of oil : methanol molar ratio, 5 wt% of catalyst, 60°C). However, the best results were obtained for the antigorite and chrysotile precursors, which are discussed in terms of mineral structure and the more efficient formation of the active phase K2MgSiO4.

关键词： silicate serpentinite lizardite antigorite talc chrysotile biodiesel

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Quantitative Study on Nonmetallic Inclusion Particles in Steels by Automatic Image Analysis With Extreme Values Method

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Journal of Iron and Steel Research(International) 2009年第4期16卷 18-21,32页

作者： Cássio Barbosa José Brant de Campos Jneo Lopes do Nascimento Iêda Maria vieira Caminha National Institute of Technology

The presence of nonmetallic inclusion particles which appear during steelmaking process is harmful to the properties of steels, which is mainly as a function of some aspects such as size, volume fraction, shape, and distribution of these particles. The automatic image analysis technique is one of the most important tools for the quantitative determination of these parameters. The classical Student approach and the Extreme Values Method （EVM） were used for the inclusion size and shape determination and the evaluation of distance between the inclusion particles. The results thus obtained indicated that there were significant differences in the characteristics of the inclusion particles in the analyzed products. Both methods achieved results with some differences, indicating that EVM could be used as a faster and more reliable statistical methodology.

关键词： image analysis steel microstructure nonmetallic inclusion

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Different culture conditions applied to in vitro shoot multiplication of two Eucalyptus benthamii explant sources

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Journal of Forestry Research 2020年第3期31卷 857-869页

作者： Natalia Pimentel Esposito-Polesi Leandro Silva de Oliveira Francisco Jose Benedini Baccarin Cristina vieira de Almeida Marcilio de Almeida Biological Science Department "Luiz de Queiroz"Superior College of AgricultureUniversity of Sao Paulo(ESALQ/USP)Padua Dias Avenue11P.O.Box 9PiracicabaSP 13418-900Brazil Institute of Agriculture Sciences Federal University of Minas Gerais(UFMG)Universitaria Avenue1000Montes ClarosMG 39404-547Brazil State University of Goias(UEG) Rua S7Qd.1S SulPalmeiras de GoiasGO 76190-000Brazil In vitro Palm Consultoria Estudo e Desenvolvimento Biologico LtdaRua Itajobi421PiracicabaSP 13432-000Brazil

Eucalyptus adult material requires more successive subcultures in the in vitro multiplication phase for increased vigor and cellular activity. This study evaluated the endophytic manifestation and shoot multiplication of one 13-year-old Eucalyptus benthamii clone under different culture conditions and used canopy branches(CB) and trunk base material as explant sources. The culture media were wood plant medium(WPM), Murashige and Skoog medium(MS) and JADS(Correia and co-authors medium).Based on the results of the initial multiplication experiment, further tests examined sucrose concentrations and p H. Morphophysiology, dry mass production, endophyticmanifestation and histochemical were determined. Explant sources responded differently to MS and JADS media, but the WPM medium promoted homogeneous *** responses were similar for both explant sources when sucrose concentrations varied. Shoots died in the absence of sucrose, showed high oxidation at 60 g L-1 and optimal development at 30 g L-1. Endophytes were more evident for shoots from the CB origin. Explant sources responded distinctively to treatment due to physiological and intrinsic genetic factors. Therefore, explant sources, different culture media, sucrose concentration and p H may determine micropropagation success and influence the presence and/or intensity of endophytic manifestation.

关键词： Plant tissue culture Explant sources Culture media Sucrose pH

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Energy Efficiency Technologies for Hydroelectric Power Plants: A Case Study in Brazil

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Journal of Power and Energy Engineering 2022年第5期10卷 90-115页

作者： Thiago Averaldo Bimestre José Antonio Mantovani Júnior Eliana vieira Canettieri Celso Eduardo Tuna Pedro Magalhães Sobrinho Chemistry and Energy Department School of Engineering S&#227o Paulo State University UNESP Guaratinguetá Brazil Center for Weather Forecasting and Climate Studies National Institute for Space Research CPTEC/INPE Cachoeira Paulista Brazil

Hydroelectricity has great importance for the global macroeconomy. In Brazil, hydroelectricity has been highlighted as the main source of generation of the electric system both for its economic competitiveness and for the abundance of this energy resource. Based on a diagnosis methodology, this article presents a case study in a Brazilian hydroelectric plant in order to optimize the use of energy and propose improvements regarding the rationalization of its application. The systems associated with energy generation were evaluated and the results proved to be potentially advantageous with an estimated savings of 2910 MWh/year in the electricity consumption of the installation itself, with better use of the equipment and the possibility of increasing the power generated.

关键词： Hydropower Plant Renewable Energy Technology Energy Efficiency Energetic Diagnosis Sustainable

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