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Inactivation of SACE_3446, a TetR family transcriptional regulator, stimulates erythromycin production in Saccharopolyspora erythraea

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Synthetic and Systems Biotechnology 2016年第1期1卷 39-46页

作者： Hang Wu Yansheng Wang Li Yuan Yongrong Mao Weiwei Wang Lin zhu panpan Wu Chengzhang Fu Rolf Muller David T.Weaver Lixin Zhang Buchang Zhang Institute of Health Sciences School of Life SciencesAnhui UniversityHefei 230601China CAS Key Laboratory of Pathogenic Microbiology&Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China Helmholtz Institute for Pharmaceutical Research Helmholtz Centre for Infection Research and Department of Pharmaceutical BiotechnologySaarland UniversityP.O.Box 1511566041 SaarbruckenGermany

Erythromycin A is a widely used antibiotic produced by Saccharopolyspora erythraea;however,its biosynthetic cluster lacks a regulatory gene,limiting the yield enhancement via regulation engineering of ***,six TetR family transcriptional regulators(TFRs)belonging to three genomic context types were individually inactivated in *** A226,and one of them,SACE_3446,was proved to play a negative role in regulating erythromycin *** and qRT-PCR analysis revealed that SACE_3446 covering intact N-terminal DNA binding domain specifically bound to the promoter regions of erythromycin biosynthetic gene eryAI,the resistant gene ermE and the adjacent gene SACE_3447(encoding a longchain fatty-acid CoA ligase),and repressed their ***,we explored the interaction relationships of SACE_3446 and previously identified TFRs(SACE_3986 and SACE_7301)associated with erythromycin *** demonstrated relatively independent regulation mode of SACE_3446 and SACE_3986 in erythromycin biosynthesis,we individually and concomitantly inactivated them in an industrial *** *** with WB,the WBΔ3446 and WBΔ3446Δ3986 mutants respectively displayed 36%and 65%yield enhancement of erythromycin A,following significantly elevated transcription of eryAI and *** cultured in a 5 L fermentor,erythromycin A ofWBΔ3446 and WBΔ3446Δ3986 successively reached 4095 mg/L and 4670 mg/L with 23%and 41%production improvement relative to *** strategy reported here will be useful to improve antibiotics production in other industrial actinomycete.

关键词： Saccharopolyspora erythraea Erythromycin TetR family SACE_3446 Regulatory network

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Interactive effects of warming and eutrophication on zooplankton could reverse the stoichiometric mismatch with phytoplankton

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Water Biology and Security 2023年第4期2卷 45-52页

作者： Konghao zhu Huan Zhang Peiyu Zhang panpan Wang Hailu Li Mingjun Feng Huan Wang Hongxia Wang Min Zhang Jun Xu State Key Laboratory of Freshwater Ecology and Biotechnology of China Institute of HydrobiologyChinese Academy of SciencesWuhan430072China College of Fisheries Huazhong Agricultural UniversityEngineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic BeltMinistry of EducationHubei Provincial Engineering Laboratory for Pond AquacultureWuhanChina Key Laboratory of Poyang Lake Environment and Resource Utilization of Ministry of Education Nanchang UniversityNanchangChina University of Chinese Academy of Sciences Beijing100049China

The core ecosystem functioning(*** transfer efficiency)is at risk of being disrupted by the growing mismatch between nutrient content of primary producers and nutrient demand of grazing *** stoichiometry provides a conceptual framework that explains this trophic interaction using C,N and P elemental composition across trophic *** light of ongoing climate change and eutrophication,previous studies have raised concerns regarding the growing stoichiometric mismatch between phytoplankton and zooplankton,given the stoichiometric plasticity of ***,there is currently little conclusive evidence on the stoichiometric mismatch from a dual perspective of phytoplankton and *** address this,we conducted a mesocosm experiment to investigate the separate and combined effects of climate warming(a constant increase of t3.5C plus heat waves)and eutrophication(nutrient addition)on stoichiometric mismatch between phytoplankton and zooplankton by examining stoichiometric changes in both *** observed a growing trend in stoichiometric mismatches when warming or nutrient addition acted individually,which was mediated by the increase in nutrient demand(N,P elements)of zooplankton ***,when these stressors acted jointly,the mismatches were *** could be because climate warming and eutrophication combined would lead to changes in species composition,which accordingly reshaped the stoichiometric composition at the community *** results illustrate the need of stoichiometric mismatches for understanding the implication of global change on trophic interactions and ecosystem functioning,requiring consideration not only of cross-trophic levels but also of compositional changes within communities.

关键词： Climate warming Ecological stoichiometry Eutrophication Stoichiometric mismatch Trophic interaction

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Techno-economic analysis of MJ class high temperature Superconducting Magnetic Energy Storage(SMES) systems applied to renewable power grids

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Global Energy Interconnection 2018年第2期1卷 172-178页

作者： Jiahui zhu panpan Chen Chenghong Gu Hongjie Zhang Jianwei Li Huiming Zhang Ming Qiu Jianlin Li Weijia Yuan Ignacio Hernando Gil China Electric Power Research Institute Beijing 100192China Department of Electronic and Electrical Engineering University of BathUnited Kingdom

High temperature Superconducting Magnetic Energy Storage(SMES) systems can exchange energy with substantial renewable power grids in a small period of time with very high efficiency. Because of this distinctive feature, they store the abundant wind power when the power network is congested and release the energy back to the system when there is no congestion. However, considering the cost and lifespan of SMES systems, there is an urgent demand to conduct a cost-benefit analysis to justify its role in smart grid development. This study explores the application and performs economic analysis of a 5 MJ SMES in a practical renewable power system in China based on the PSCAD/EMTDC software. An optimal location of SMES in Zhangbei wind farm is presented using real power transmission parameters. The stabilities of the renewable power grid with and without SMES are discussed. In addition, a financial feasibility study is conducted by comparing the cost and the savings from wind power curtailment of deploying SMES and battery. The economic analysis tries to find the balance between SMES investment cost and wind farm operation cost by using real data over a calendar year. The technical analysis can help guide the optimal allocation of SMES for compensating power system instability with substantial wind power. Further, the economic analysis provides a useful indication of its practical application feasibility to fight the balance between cost and benefit.

关键词： High temperature Superconducting Magnetic Energy Storage (SMES) system Renewable energy Smart grid Allocation planning Economic planning Large-scale energy storage

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Effectiveness associated with different therapies for senile osteoporosis: a network Meta-analysis

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Journal of Traditional Chinese Medicine 2020年第1期40卷 17-27页

作者： Wang Haixia Mo Shu Yang Li Wang panpan Sun Kehuan Xiong Yingquan Liu Hengrui Liu Xiaoguang Wu Zhidi Ou Ling Li Xiaoyun Peng Xunqian Peng Bojia He Haibin Tian Ya Zhang Ronghua zhu Xiaofeng School of Traditional Chinese Medicine Jinan UniversityGuangzhou 510632China College of Pharmacy Jinan UniversityGuangzhou 510632China Cancer Research Institute and School of Pharmacy Jinan UniversityGuangzhou 510632China Department of Traditional Chinese Medicine the First Affiliated Hospital of Jinan UniversityGuangzhou 510632China

OBJECTIVE:The aim of this study was to evaluate the effectiveness of Chinese herbal medicines for invigorating the kidney(CHMIK)on senile ***:We searched for studies in English-language databases(Pub Med,the Cochrane Library,and Web of Science)and Chinese-language databases(China National Knowledge Infrastructure,Wan Fang Data,VIP Chinese periodical service platform),and China Biology Medicine disc from their inception to September *** controlled trials comparing the effectiveness of Traditional Chinese Medicine therapies(alone or in combination)and conventional clinical medicine therapies among older adult patients with osteoporosis were *** conducted a network Meta-analysis with a Bayesian hierarchical random-effects model using RStudio software,Version ***:Forty-three randomized controlled trials assessing the differences between Traditional Chinese Medicine and conventional clinical medicine were identified,including 15 treatments and involving 3316 *** results of the network Meta-analysis indicated that alendronate(odds ratio[OR]=0.20,95%confidence interval[CI]:0.047-0.73)and calcium(OR=0.18,95%CI:0.11-0.30)are significantly more effective if combined with oral *** alone is significantly more effective than both alendronate(OR=0.34,95%CI:0.10-1.0)and calcium(OR=0.13,95%CI:0.056-0.28).Moreover,CHMIK+tuina+calcium is more effective than CHMIK+calcium+vitamin D+alendronate(OR=18.0,95%CI:1.1-2.7 e+02).CONCLUSION:The present network Meta-analysis found that alendronate and calcium are more effective if combined with oral CHMIK and that oral CHMIK alone may be more effective than alendronate or *** may have an advantage over oral *** CHMIK and calcitonin show the most potential for treating senile osteoporosis.

关键词： Osteoporosis Aged Reinforcing kidney Traditional Chinese drugs Alendronate Calcium Calcitonin Network Meta-analysis

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Functional characterization of powdery mildew resistance gene MIIW172,a new Pm60 allele and its allelic variation in wild emmer wheat

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Journal of Genetics and Genomics 2022年第8期49卷 787-795页

作者： Qiuhong Wu Yongxing Chen Beibei Li Jing Li panpan Zhang Jingzhong Xie Huaizhi Zhang Guanghao Guo Ping Lu Miaomiao Li Keyu zhu Wenling Li Tzion Fahima Eviatar Nevo Hongjie Li Lingli Dong Zhiyong Liu State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyThe Innovative Academy of Seed DesignChinese Academy of SciencesBeijing 100101China College of Advanced Agricultural Sciences University of Chinese Academy of SciencesBeijing 100049China Beijing Advanced Innovation Center for Tree Breeding by Molecular Design Beijing University of AgricultureBeijing 102206China Institute of Evolution and the Department of Evolutionary and Environmental Biology University of HaifaMt.CarmelHaifa 3498838Israel The National Engineering Laboratory of Crop Molecular Breeding Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

Wild emmer wheat(Triticum dicoccoides,WEW)is an immediate progenitor of both the cultivated tetraploid and hexaploid wheats and it harbors rich genetic diversity against powdery mildew caused by Blumeria graminis ***(Bgt).A powdery mildew resistance gene Ml I^(W172)originated from WEW accession I^(W172)(G-797-M)is fine mapped in a 0.048 centimorgan(c M)genetic interval on 7 AL,corresponding to a genomic region spanning 233 kb,1 Mb and 800 kb in Chinese Spring,WEW Zavitan,and *** G1812,*** I^(W172)encodes a typical NLR protein NLRI^(W172)and physically locates in an NBS-LRR gene ***^(W172)is subsequently identified as a new allele of Pm60,and its function is validated by EMS mutagenesis and transgenic *** analysis of the Pm60 alleles reveals diversifications in sequence variation in the locus and presence and absence variations(PAV)in WEW *** common single nucleotide variations(SNV)are detected between the Pm60 alleles from WEW and ***,indicative of speciation divergence between the two different wheat *** newly identified Pm60 alleles and haplotypes in WEW are anticipated to be valuable for breeding powdery mildew resistance wheat cultivars via marker-assisted selection.

关键词： Blumeria graminis f.sp.tritici Triticum dicoccoides Presence and absence variation Pm60 Allelic variation

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基于集成CART算法的TBM掘进参数与围岩等级预测

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岩石力学与工程学报 2020年第9期39卷 1860-1871页

作者：朱梦琦朱合华王昕程盼盼同济大学土木工程学院上海200092 同济大学土木工程防灾国家重点实验室上海200092

全断面隧道掘进机(TBM)运行过程中将会产生大量可以反映当时地质状况的数据,旨在充分利用TBM运行数据,使用基于集成CART算法的随机森林和AdaCost,实现岩体信息实时感知与掘进参数智能优化。为建立快速、准确的TBM掘进参数与围岩等级预... 详细信息

全断面隧道掘进机(TBM)运行过程中将会产生大量可以反映当时地质状况的数据,旨在充分利用TBM运行数据,使用基于集成CART算法的随机森林和AdaCost,实现岩体信息实时感知与掘进参数智能优化。为建立快速、准确的TBM掘进参数与围岩等级预测模型,提出了一种掘进模式识别方法,将TBM掘进循环分为空推段、上升段与稳定段;并使用随机森林模型和上升段前30 s数据,实时预测稳定掘进时掘进推力与刀盘扭矩的取值,预测精度分别达到0.90和0.87;继而使用代价敏感的AdaCost算法预测围岩等级,解决了传统机器学习算法不适用于岩体级别数据不均衡的场景,相较于随机森林模型,对于IV级和V级岩体的预测正确率分别提升了16%与50%。此外,总推进力、刀盘功率、刀盘扭矩、推进速度等TBM运行参数被证明与TBM掘进情况密切相关;刀盘转速、撑靴压力、撑靴俯仰角、推进速度和撑靴滚动角等参数被证明能更好地反映围岩状况。这些认识与成果对TBM掘进参数优化和风险预警有着重要意义,并将为建立基于数据挖掘的TBM智能决策控制平台提供参考。

关键词：岩石力学 TBM掘进参数围岩等级不均衡数据集随机森林 AdaCost算法

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Clinical Analysis of Pectoralis Major Myocutaneous Flap for Reconstruction of Tissue Defects after Pharyngolaryngeal Cancer Surgery

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Surgical Science 2021年第4期12卷 135-142页

作者： Fengjuan zhu Hua Wang Jianyun Zhao Yan Wang panpan Cui Jiansheng Li Department of Otolaryngology People’s Hospital of Rizhao Rizhao China

Objective: To explore the methods and effects of pectoralis major myocutaneous flap in pharyngolaryngeal cancer surgery. Methods: Among 23 cases of pharyngolaryngeal cancer patients, the surgical procedure was based on pathologic conditions, and the surgical defects were repaired by pectoralis major myocutaneous flap. Results: Among 23 patients, there were 1 case of skin flap necrosis, 4 cases of pharyngeal fistula, 1 case of hypopharyngeal esophageal stenosis;except 1 case of pharyngeal fistula died of carotid artery rupture caused by infection, 1 case of hypopharyngeal esophageal stenosis required a gastric tube, and the rest patient’s swallowing function is normal. Conclusion: The blood supply of pectoralis major myocutaneous flap is reliable with the tissue volume enough for reconstruction. It turns out to improve patient survival and postoperative quality of life with the application of pharyngolaryngeal cancer surgery.

关键词： Pectoralis Muscles Surgical Flaps Pharyngolaryngeal Neoplasms Reconstruction Curative Effect

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高速公路车辆防追尾碰撞系统的设计与控制研究

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汽车工程 2014年第9期36卷 1080-1084,1116页

作者：尹小琴朱喜行张盼盼江苏大学智能机械及机器人研究所镇江212013

针对汽车追尾碰撞事故频发和事故中驾乘人员头颈部易受损伤等问题,利用激光雷达测距和机电控制相结合的技术,提出了一种车辆防追尾碰撞系统,对高位制动灯和主动式头枕进行控制。首先利用激光雷达和本车状态信息对行车环境进行预测;接着... 详细信息

针对汽车追尾碰撞事故频发和事故中驾乘人员头颈部易受损伤等问题,利用激光雷达测距和机电控制相结合的技术,提出了一种车辆防追尾碰撞系统,对高位制动灯和主动式头枕进行控制。首先利用激光雷达和本车状态信息对行车环境进行预测;接着对后车分液压和气压两种制动形式进行行车安全车距的分析,推导出临界安全车距的计算公式;最后通过AT89C51单片机系统预设的程序,将实际行车间距与临界安全车距进行比较,并据此控制高位制动灯的开闭和主动式头枕控制系统的执行。所提出的方法有较好的工程实用性。

关键词：追尾碰撞设计控制激光雷达安全车距

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Effects of perinatal stress on the metabolites and lipids in plasma of dairy goats

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Stress Biology 2023年第1期3卷 126-144页

作者： Yan Huang Yezi Kong Bowen Li Chenxu Zhao Juan J.Loor panpan Tan Yang Yuan Fangyuan Zeng Xiaoyan zhu Simeng Qi Baoyu Zhao Jianguo Wang College of Veterinary Medicine Northwest A&F UniversityYangling 712100ShaanxiChina LipidALL Technologies Company Limited Changzhou 213022JiangsuChina Department of Animal Sciences Division of Nutritional SciencesUniversity of IllinoisUrbanaIL 61801USA

Dairy goats experience metabolic stress during the peripartal period,and their ability to navigate this stage of lactation is related to the occurrence and development of metabolic *** dairy cows,there is a lack of comprehensive analysis of changes in the plasma profiles of peripartal dairy goats,particularly using high-throughput techniques.A subset of 9 clinically-healthy dairy goats were used from a cohort of 96 primiparous Guanzhong dairy goats(BCS,2.75±0.15).Blood samples were collected at seven time points around parturition(d 21,14,7 before parturition,the day of kidding,and d 7,14,21 postpartum),were analyzed using untargeted metabolomics and targeted *** orthogonal partial least squares discriminant analysis model revealed a total of 31 differential metabolites including p-cresol sulfate,pyruvic acid,cholic acid,and oxoglutaric *** pathway enrichment analysis identified phenylalanine metabolism,aminoacyl-tRNA biosynthesis,and citrate cycle as the top three significantly-altered *** Limma package identified a total of 123 differentially expressed ***(PS),free fatty acids(FFA),and acylcarnitines(ACs)were significantly increased on the day of kidding,while diacylglycerols(DAG)and triacylglycerols(TAG)***(Cer)and lyso-phosphatidylinositols(LPI)were significantly increased during postpartum period,while PS,FFA,and ACs decreased postpartum and gradually returned to antepartum *** species of FFA and phosphatidylcholines(PC)were segregated based on the differences in the saturation and length of the carbon ***,this work generated the largest repository of the plasma lipidome and metabolome in dairy goats across the peripartal period,which contributed to our understanding of the multifaceted adaptations of transition dairy goats.

关键词： Dairy goat Transition period Untargeted metabolomics Lipidomics

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Ionic liquid on zirconia-modified silica as catalyst for oxidative desulfurization

Ionic liquid on zirconia-modified silica as catalyst for oxi...

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第十届全国催化剂制备科学与技术研讨会（成都）

作者： panpan Zhang Aili Liu Bao Wang Mingyuan zhu Bin Dai School of Chemistry and Chemical Engineering of Shihezi University Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan School of Chemistry and Chemical Engineering Shihezi University

In our work, the ionic liquid on zirconia-modified silica was studied as a catalyst for oxidative desulfurization. [BMIM]PF6/ZrO–SiO catalyst was successfully prepared by immobilizing Ionic liquid(1-butyl-3-methylimidazolium hexafluorophosphate, [BMIM]PF6) on zirconia-modified silica and employed as a catalyst for oxidative desulfurization(ODS). The optimum conditions include the loading of the ionic liquid, the reaction temperature and time, the O/S(HO/sulfur) molar ratio and the catalyst dosage. Under the optimal reaction conditions, the sulfur removal rate from dibenzothiophene was nearly 100%.

关键词： catalyst oxidative desulfurization Ionic liquid

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