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Food Innovation and Advances 2023年第4期2卷 291-301页

作者： Xuan Wang pamela owusu osei Lei Rao Xiaomeng Wu Xiaojun Liao College of Food Science and Nutritional Engineering National Engineering Research Center for Fruit&Vegetable ProcessingKey Laboratory of Fruit&Vegetable ProcessingMinistry of Agriculture and Agricultural AffairsBeijing Key Laboratory for Food Non-thermal ProcessingChina Agricultural UniversityBeijing 100083China

The Leuconostoc citreum SJ-57 strain isolated from the sweet potato starch production showed great potential as a microbiological flocculant,but its underlying flocculation mechanisms are yet *** this study,infrared spectroscopy and thermodynamic analysis were performed to elucidate the short-range and long-range interactions between *** SJ-57 and starch granules,revealing that bacteria cells bond starch granules via metal-bridging ionic bonds.A high repulsive energy barrier of~8×10^(−18)J must be overcome to initiate the flocculation ***,protease,lipase,lysozyme,dextranase,and guanidine hydrochloride were used to treat the bacterial cell,confirming that its flocculation ability originated from surface proteins,including GW structural domain proteins,DnaK,GroEL,elongation factor Tu,and lysozyme *** primary flocculation mechanisms of *** was proposed to provide a deep understanding of microbiological flocculants and a foundation for future industrial applications in starch production.

关键词： starch overcome originated

Anti-glaucoma potential of Heliotropium indicum Linn in experimentally-induced glaucoma

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Eye and Vision 2015年第1期2卷 162-169页

作者： Samuel Kyei George Asumeng Koffuor Paul Ramkissoon osei owusu-Afriyie Discipline of Optometry School of Health SciencesCollege of Health SciencesUniversity of KwaZulu-NatalDurbanSouth Africa Department of Optometry School of Physical SciencesUniversity of Cape-CoastCape-CoastGhana Department of Pharmacology Faculty of Pharmacy and Pharmaceutical SciencesKwame Nkrumah University of Science and TechnologyKumasiGhana Department of Pathology Komfo Anokye Teaching HospitalKumasiGhana

Background:Heliotropium indicum is used as a traditional remedy for hypertension in *** aim of the study was to evaluate the anti-glaucoma potential of an aqueous whole plant extract of *** to manage experimentally-induced ***:The percentage change in intraocular pressure(IOP),after inducing acute glaucoma(15 mLkg^(−1) of 5%dextrose,i.v.),in New Zealand White rabbits pretreated with Heliotropium indicum aqueous extract(HIE)(30–300 mgkg^(−1)),acetazolamide(5 mgkg^(−1)),and normal saline(10 mLkg^(−1))per os were *** were also monitored in chronic glaucoma in rabbits(induced by 1%prednisolone acetate drops,12 hourly for 21 days)after treatments with the same doses of HIE,acetazolamide,and normal saline for 2 *** anti-oxidant property of the extract was assessed by assaying for glutathione levels in the aqueous *** concentration in the vitreous humour was also determined using ELISA *** assessment of the ciliary bodies was ***:The extract significantly reduced intraocular pressure(p≤0.05–0.001)in acute and chronic glaucoma,preserved glutathione levels and glutamate concentration(p≤0.01–0.001).Histological assessment of the ciliary body showed a decrease in inflammatory infiltration in the extract and acetazolamide-treated group compared with the normal saline-treated ***:The aqueous whole plant extract of Heliotropium indicum has ocular hypotensive,anti-oxidant and possible neuro-protective effects,which therefore underscore its plausible utility as an anti-glaucoma drug with further investigation.

关键词： Glutathione assay Steroid-induced glaucoma Acetazolamide New Zealand white rabbit Anti-glaucoma drug Glutamate

Floral diversity and carbon stocks and of protected forest ecosystem: A case of UENR’s Bat Sanctuary, Sunyani, Ghana

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Open Journal of Forestry 2018年第1期8卷 29-41页

作者： Nat owusu-Prempeh osei owusu Antobre Thomas Agyei Department of Land Reclamation and Rehabilitation Faculty of Forest Resources Technology Kwame Nkrumah University of Science and Technology Kumasi Ghana Global Change Research Institute CzechGlobe Brno Czech Republic University of Energy and Natural Resources (UENR) Sunyani Ghana

The study assesses the functional status of the University of Energy and Natural Resources’ (UENR) bat sanctuary by examining its floral diversity and carbon stocks. Twenty-nine sampling points (plots) were randomly generated by using the ArcGIS random sampling algorithm. Using a three-nest sampling plot of 100m2, 25m2, and 1m2 quadrat, the enumeration of trees (DBH>10cm), saplings (>2cm DBH 0.05) between the 10m buffer created and the core area with respect to species diversity and carbon stocks. The study has provided valuable information on the functional status of the bat sanctuary which will help promote its conservation for sustained provision of ecosystem services.

关键词： Carbon Stocks Floral Species Diversity Protected Area (PA) Edge Effects Geostatistics Ghana

Optimized Recovery of DNA and Subsequent Short Tandem Repeat Profiling of Different Tissues Sampled from Embalmed Human Cadavers

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Journal of Forensic Science and Medicine 2023年第4期9卷 309-319页

作者： Kofi Adjapong Afrifah Alexander Badu-Boateng Samuel Antwi-Akomeah osei owusu-Afriyie Mishael Yankey Biology/DNA Unit Forensic Science LaboratoryCID HeadquartersGhana Police ServiceSchool of MedicineUniversity of GhanaLegonGhana Department of Forensic Sciences Columbian College of Arts and SciencesThe George Washington UniversityWashingtonDCUSA Department of Pathology Ghana Police HospitalAccraSchool of MedicineUniversity of GhanaLegonGhana Department of Pathology School of Medicine and DentistryKwame Nkrumah University of Science and Technology(KNUST)KumasiSchool of MedicineUniversity of GhanaLegonGhana Department of Community Health School of MedicineUniversity of GhanaLegonGhana

Introduction:Storage of specimens sampled from human remains for pathological testing,embalming for burial purposes,and for human identification often requires formalin fixation and/or paraffin *** knowledge in molecular biology techniques and forensic DNA analysis makes it possible to optimize the extraction of amplifiable DNA from formalin-fixed tissues by improving the pre-treatment,optimizing the digestion condition of proteinase K,simplifying the extraction protocol and purifying the extracted DNA with optimized volumes of ***:This research sought to extract amplifiable DNA from thirteen brain,bone marrow and cartilage samples from four formalin embalmed human *** and Methods:Brain,cartilage and bone marrow samples were taken from four different cadavers at autopsy at the Ghana Police Hospital mortuary in Accra,Ghana sixty-two days after *** optimized preparation and DNA extraction protocol was carried out on all the *** samples were also taken from a non-formalin treated fifth cadaver of known STR profile,and standard DNA extraction performed to serve as positive ***:Our optimized protocol yielded detectable quantities of DNA from the samples when quantified with the 7500 Real-Time PCR *** extracted DNA also yielded full STR profiles with varying peak heights for forensic identification *** measured degradation indexes of the DNA samples were greater than 1.0,with peak heights of generated STR profiles above the limits of detection of the 3500 genetic ***:Our current study demonstrated an optimized method of DNA extraction from tissues(brain,cartilage and bone marrow)sampled from formalin embalmed human *** optimized protocol reduced the concentration of formalin fixation residues in extracted DNA from formalin-fixed tissues,thereby improving the amplification efficiency for STR ***,bone marrow and cartilages can be a good source of DNA f

关键词： Formalin fixation DNA profiling STR analysis optimized protocol embalmed cadavers

Reflecting on tuberculosis case notification and treatment outcomes in the Volta region of Ghana:a retrospective pool analysis of a multicentre cohort from 2013 to 2017

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Global Health Research and Policy 2019年第1期4卷 1-13页

作者： Eric osei Samuel Oppong Daniel Adanfo Bless Ativor Doepe Andrews owusu Augustine Goma Kupour Joyce Der Department of Population and Behavioural Sciences School of Public HealthUniversity of Health and Allied SciencesHoGhana Department of Epidemiology School of Public HealthUniversity of Health and Allied SciencesHoGhana

Background:Tuberculosis(TB)remains a petrified condition with a huge economic and health impact on families and health systems in *** of TB programme performance indicators can provide reliable data for direct measurement of TB incidence and *** study reflects on the trends of TB case notification and treatment outcomes and makes comparison among 10 districts of the Volta region of ***:This was a retrospective analysis of surveillance data of a cohort of TB cases from 2013 to *** of case notification and treatment outcomes were examined and *** regression was used to determine the independent relationship between patients and disease characteristics and unsuccessful treatment *** ratios,95%confidence intervals and p-values were ***:A gradual declining trend of case notification of all forms of TB was noticed,with an overall case notification rate(CNR)of 65 cases per 100,000 population during the period.A wide variation of case notification of TB was observed among the districts,ranging from 32 to 124 cases per 100,000 ***,treatment success rate decreased slightly from 83.1%during the first year to 80.2%in 2017,with an overall treatment success rate of 82.5%(95%CI:81.3-83.8%).Treatment failure,death,and lost to follow up rates were 0.8%(range 0.5-1.2%),13.5%(range 12.4-14.7%),and 3.1%(range 2.6-3.8%)*** treatment success rate among districts ranged from 70.5%in South Tongu to 90.8%in Krachi West *** after treatment interruption(Adjusted odds ratio[AOR]:3.62;95%CI:1.66-7.91;P90%set by the World Health Organiz

关键词： Tuberculosis Case notification Treatment outcomes Volta region Ghana

Orofacial Cysts at Komfo Anokye Teaching Hospital, Ghana

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Surgical Science 2013年第1期4卷 65-67页

作者： Alexander Acheampong Oti Peter Donkor osei owusu-Afriyie Komfo Anokye Teaching Hospital Kumasi Ghana Kwame Nkrumah University of Science and Technology Kumasi Ghana

Background: Information about orofacial cysts from African populations is scarce and there are only a few studies available regarding the prevalence of these lesions in the West African sub-region. The purpose of the present study is to determine the distribution and prevalence of all histologically diagnosed orofacial cysts in Kumasi, Ghana. Aim: To determine prevalence, sex, age and anatomic distribution of orofacial cyst seen at the oral and maxillofacial unit in Komfo Anokye Teaching hospital (KATH). Method: This is a retrospective study, which examined histologically diagnosed lesions including orofacial cysts. The study duration was from 1999 to 2010 September inclusive. Results: There were 37 odontogenic cysts constuting 6.5%, of all orofacial lesions. There were 18 non-odontogenic cysts i.e. 3.1% of all lesions diagnosed during the study period. The odontogenic cysts comprised 19 (51.4%) developmental cysts and 18 (48.6%) inflammatory cysts. Male-to-female ratio for the orofacial cysts was 1:1 and the mean age was 36.7 years. Conclusion: There is low prevalence of the odontogenic cysts, which is consistent with findings from other African studies. Although radicular cysts accounted for the majority of orofacial cysts in this study, the prevalence of radicular cysts is low compared to reports from developed countries.

关键词： Orofacial Cyst Radicular Odontogenic

Does Environmental Laws and Policies Work?A Review Of Ghana’s Case

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Research in Ecology 2020年第3期2卷 32-41页

作者： Aboagye Emmanuel Mensah Attobrah Justice owusu Nana osei Fletcher Henrietta Zhongnan University of Economics and Law WuhanHubei430000China Ocean University of China QingdaoShandong266000China

Enforcing environmental laws and policies has been one of the pressing agenda *** the mandate given to the Environmental Protection Agency(EPA)by the Act 490,there are still numerous environmental issues unaddressed in *** paper is geared towards unravelling and identifying the current additions that have been made to existing literature on environmental laws and policies in *** paper made a wide-ranging general literature review to outline significant contributions concerning environmental laws and *** environmental laws and policies were identified but have been fragmented and are not codified in a particular *** is evident from the review of literature existing that there are still areas for advanced research on the topic of environmental laws and policies placing more emphasis on institution concerned with environmental issues at global *** study recommended the need for capacity building for environmental personnel and data due to the numerous technological advancement to better assist the implementation and enforcement of environmental laws in Ghana.

关键词： Environmental laws Environmental policies Environmental Protection Agency Law enforcement Act 490 Ghana

Salivary Gland Tumours at Komfo Anokye Teaching Hospital, Ghana

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Surgical Science 2013年第2期4卷 135-139页

作者： Alexander Acheampong Oti Peter Donkor Solomon Obiri-Yeboah osei Afriyie-owusu Komfo Anokye Teaching Hospital Kumasi Ghana

In African studies on salivary gland tumours, there are considerable epidemiological differences in different parts of the continent. There is no study of salivary gland tumours from the second largest hospital in Ghana, Komfo Anokye Teaching Hospital. Aim: This study was to look at the prevalence and demographic distribution of salivary gland tumours at the Komfo Anokye Teaching Hospital (KATH). Method: Histopathologically diagnosed salivary gland tumours of consecutive patients were evaluated. The study duration was from 1999 to 2010 October. Result: The total number of salivary gland tumours were 121. Male to female ratio was 1.75:1. Out of the total of 34 salivary malignancies seen, male to female ratio was 1.8:1. Malignant tumour was 28.1% while 71.9% were benign. Mean age for malignancy was 53.5 years (SD = 9.7) and that for benign was 35.5 years (SD = 8.2). Conclusion: The commonest benign tumour of the parotid was Pleomorphic adenoma (48.3%) which is consistent with most of the African and western reports. Warthin’s tumour prevalence was higher than most of the studies from Africa.

关键词： Salivary Gland Pleomorphic Benign

A Six-Year Review of Head and Neck Cancers at the Komfo Anokye Teaching Hospital, Kumasi, Ghana

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International Journal of Otolaryngology and Head & Neck Surgery 2014年第5期3卷 271-278页

作者： Rita Larsen-Reindorf osei owusu-Afriyie Alexander Oti Acheampong Isaac Boakye Baffour Awuah Department of ENT Komfo Anokye Teaching Hospital Kumasi Ghana Department of Pathology Komfo Anokye Teaching Hospital Kumasi Ghana Department of Oral and Maxillofacial Unit Komfo Anokye Teaching Hospital Kumasi Ghana Research & Development Unit Komfo Anokye Teaching Hospital Kumasi Ghana Directorate of Oncology Komfo Anokye Teaching Hospital Kumasi Ghana

Background: In Ghana, information on the prevalence and pattern of Head and Neck Cancers (HNCA) is scarce. There are few publications based on specific sub-sites of the head and neck, however, literature on the prevalence and pattern of HNCA in general is lacking. The present study aimed to describe the pattern of HNCA among patients seen at the multidisciplinary HNCA clinic of Komfo Anokye Teaching Hospital. Methods: This is a retrospective review of all consecutive patient records seen at the multidisciplinary clinic from 2004 to 2009. Results: There were 149 males and 103 females giving a male to female ratio of 1.45:1. The age range was 2 - 95 years with a mean of 48.1 years. The commonest type of HNCA in this review is cancers of the oral cavity (29.4%), followed by accessory sinuses (13.9%) and larynx (13.5%). Nasopharyngeal cancer affected young people, with 34.5% of affected patients aged between 10 and 19 years. The commonest histopathological type was squamous cell carcinoma (49.6%). Majority (47.6%) of patients presented at stage IV. Conclusion: The commonest HNCA is oral cancer. Majority of patients with HNCA are presenting late calling for public health education to raise awareness and promote early detection.

关键词： Head and Neck Cancer Oral Cavity Squamous Cell Carcinoma

Climatic and non-climatic factors driving the livelihood vulnerability of smallholder farmers in Ahafo Ano North District,Ghana

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Regional Sustainability 2024年第3期5卷 24-39页

作者： Frank BAFFOUR-ATA Louisa BOAKYE Moses Tilatob GADO Ellen BOAKYE-YIADOM Sylvia Cecilia MENSAH Senyo Michael KWAKU KUMFO Kofi Prempeh osei owusu Emmanuel CARR Emmanuel DZIKUNU Patrick DAVIES Department of Environmental Science Kwame Nkrumah University of Science and TechnologyKumasiAK-039-5028Ghana Department of Meteorology and Climate Science Kwame Nkrumah University of Science and TechnologyKumasiAK-039-5028Ghana

Smallholder farmers in Ahafo Ano North District,Ghana,face multiple climatic and non-climatic *** study assessed the factors contributing to the livelihood vulnerability of smallholder farmers in this district by household surveys with 200 respondents and focus group discussions(FGDs)with 10 *** Mann–Kendall trend test was used to assess mean annual rainfall and temperature trends from 2002 to *** relative importance index(RII)value was used to rank the climatic and non-climatic factors perceived by *** socioeconomic characteristics affecting smallholder farmers’perceptions of climatic and non-climatic factors were evaluated by the binary logistic regression *** showed that mean annual rainfall decreased(P>0.05)but mean annual temperature significantly increased(P<0.05)from 2002 to 2022 in the *** key climatic factors perceived by smallholder farmers were extreme heat or increasing temperature(RII=0.498),erratic rainfall(RII=0.485),and increased windstorms(RII=0.475).The critical non-climatic factors were high cost of farm inputs(RII=0.485),high cost of healthcare(RII=0.435),and poor condition of roads to farms(RII=0.415).Smallholder farmers’perceptions of climatic and non-climatic factors were significantly affected by their socioeconomic characteristics(P<0.05).This study concluded that these factors negatively impact the livelihoods and well-being of smallholder farmers and socioeconomic characteristics influence their perceptions of these ***,to enhance the resilience of smallholder farmers to climate change,it is necessary to adopt a comprehensive and context-specific approach that accounts for climatic and non-climatic factors.

关键词： Smallholder farmers Livelihood vulnerability Climate change Socioeconomic characteristics Food security Ghana