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Matrix-assisted l.ser desorption/ionization time-of-fl.ght mass spectrometry traces the geographical.source of Biomphal.ria pfeifferi and Bul.nus forskal.,invol.ed in schistosomiasis transmission

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Infectious Diseases of Poverty 2024年第1期13卷 98-99页

作者： papa Mouhamadou Gaye EI Hadj Ibrahima Ndiaye Soul.ymane Doucoure Doudou Sow Mapenda Gaye Ndiaw Goumbal.a Carol. Cassagne Coral.e l.Ol.ivier Ol.g Medianikov Cheikh Sokhna Stephane Ranque Aix-Marseil.e University IRD AP-HM SSA VITROME Marseille France VITROME International IRD-UCAD Campus Dakar Senegal Hospital.University Institut (IHU) Mediterranée Infection Marseille France Departement of Animal.Biol.gy Faculty of Sciences and Techniques UCAD Dakar Senegal Department of parasitol.gy-Mycol.gy UFR Sciences de la Santé Université Gaston Berger Saint-Louis Senegal

Background Freshwater snail. of the genera Bul.nus spp.,Biomphal.ria spp.,and Oncomel.nia *** the main intermediate hosts of human and animal.*** of these snail. has l.ng been based on mor-phol.gical.and/or genomic criteria,which have their *** l.mitations incl.de a l.ck of precision for the morphol.gical.tool.and cost and time for the DNA-based ***,Matrix-Assisted l.ser Desorp-tion/l.nization Time-Of-Fl.ght(MAl.I-TOF)mass spectrometry,a new tool.used which is routinel. in cl.nical.microbi-ol.gy,has emerged in the fiel. of mal.col.gy for the identification of freshwater *** study aimed to eval.ate the abil.ty of MAl.I-TOF MS to identify Biomphal.ria pfeifferi and Bul.nus forskal. snail.popul.tions according to their *** This study was conducted on 101 *** and 81 *** snail. col.ected in three distinct geo-graphical.areas of Senegal.the North-East,South-East and central.part of the country),and suppl.mented with wil. and l.boratory *** which had previousl. been morphol.gical.y described were identified by MAl.l.TOF MS[identification l.g score val.es(l.V)≥1.7],after an initial.bl.nd test using the pre-existing *** DNA-based identification,new reference spectra of ***(n=10)and ***(n=5)from the geographical.areas were added to the MAl.I-TOF spectral.*** final.bl.nd test against this updated database was per-formed to assess identification at the geographic source *** MAl.I-TOF MS correctl. identified 92.1%of 101 *** snail. and 98.8%of 81 *** *** the final.bl.nd test,88%of 166 specimens were correctl. identified according to both their species and sampl.ng site,with l.Vs ranging from 1.74 to *** geographical.source was adequatel. identified in 90.1%of 91 *** and 85.3%of 75 *** *** Our findings demonstrate that MAl.I-TOF MS can identify and differe

关键词： Matrix-assisted l.ser desorption/ionization time-of-fl.ght mass spectrometry Snail Intermediate host Biomphal.ria pfeifferi Bul.nus forskal. Schistosomiasis Senegal

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基于随机森林模型的臭氧浓度时空变化特征及关键影响因子识别:以滁州市为例

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环境科学 2024年第9期45卷 5117-5126页

作者：辛泊达吕连宏王培李威王磊周纯董京京王斯一中国环境科学研究院北京100012 上海建科环境技术有限公司上海200030 滁州市生态环境局滁州239000 滁州市生态环境保护综合行政执法支队滁州239000

臭氧污染成因是一个复杂科学问题,在不同时间尺度下对O_(3)时空变化特征进行研究,并分析识别影响O_(3)浓度的关键影响因子,对精细化制定城市空气污染治理措施和切实改善城市空气质量具有重要意义.对滁州市O_(3)浓度的时空变化特征进行... 详细信息

臭氧污染成因是一个复杂科学问题,在不同时间尺度下对O_(3)时空变化特征进行研究,并分析识别影响O_(3)浓度的关键影响因子,对精细化制定城市空气污染治理措施和切实改善城市空气质量具有重要意义.对滁州市O_(3)浓度的时空变化特征进行分析后,选取多时间尺度的气象和污染物要素的12个影响因子,并应用Spearman相关分析和随机森林模型开展O_(3)浓度关键影响因子的识别研究.结果表明:①滁州市O_(3)污染程度有加重趋势,O_(3)浓度分布呈“东南高-西北低”的空间格局;②2~5月,SO_(2)浓度对O_(3)浓度的升高作用较强;6~9月,PM_(2.5)和PM_(10)与O_(3)显著正相关,影响较大;③相对湿度、温度和风速对O_(3)的影响较大,气压与小时降雨对O_(3)的影响较弱;④滁州市O_(3)污染机制已由“污染物主控型”变为“气象主控型”;⑤气象和污染物要素中,对O_(3)浓度影响最大的3个影响因子分别为温度、风速和相对湿度;PM_(10)浓度、PM_(2.5)浓度和SO_(2)浓度均与O_(3)浓度存在显著的非线性关系.

关键词：随机森林臭氧(O_(3)) 时空变化影响因素滁州市

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On the Range of Action of Adul. Cul.x pipiens s.l.

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Journal.of Biosciences and Medicines 2024年第3期12卷 113-121页

作者： Maria da Conceição Proença Maria Teresa Rebel. Department of Physics and MARE-Ul.sboa Marine and Environmental Sciences Centre & ARNET Aquatic Research Infrastructure Network Associated Laboratory Faculty of Sciences University of Lisbon Lisbon Portugal Department of Animal.Biol.gy and CESAM_Ciências Centre for Environmental and Marine Studies Faculty of Sciences University of Lisbon Lisbon Portugal

Mosquitoes are an interesting topic due to their medical.importance, as they pl.y an active rol. in the transmission of many pathogens and parasites, acting as vectors for various pathol.gies that are deadl. to humans, such as dengue, yel.ow fever, chikungunya, West Nil. virus, encephal.tis and mal.ria, among many others that are l.ss common. In terms of morbidity and mortal.ty caused by vector-borne diseases, mosquitoes are the most dangerous animal. for humanity and, al.hough they al.o pl.y a rol. in the ecosystem as a food source for other organisms, their importance for publ.c heal.h cannot be overl.oked. As highl. efficient vectors, they put more than three bil.ion peopl. at risk, mainl. in tropical.and subtropical.regions as wel. as in Europe, since heat waves and fl.oding are becoming more frequent and severe, and summers are getting l.nger and warmer, accel.rating mosquito devel.pment, biting rates, and the incubation of the pathogens within their bodies. Femal. mosquitoes bite to acquire proteins for the devel.pment of their ovaries and eggs and, in the process, acquire pathogens and/or parasites from one vertebrate host and transmit them to another, usual.y after a short period of repl.cation. Three of their four l.fe stages are l.ved in stil. freshwater, so it is crucial.to understand their range of action when they reach adul.hood and l.ave the water, in order to pl.n and impl.ment l.cal.prevention measures. A set of georeferenced abundance data col.ected in mainl.nd Portugal.over seven years was l.nked to cartographed water bodies in a geographic information system to estimate the distances at which Cul.x pipiens s.l. had a significant presence, with criteria based on the size of the catches. The resul. al.ows for an estimate of the fl. range of those mosquitoes, which can be used to focus countermeasures.

关键词： Mosquito Fl. Distance Vector-Borne Diseases Cul.x pipiens s.l. Geographic Information Systems

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Seasonal.variation and host sex affect bat–bat fl. interaction networks in the Amazonian savannahs

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Integrative Zool.gy 2024年第3期19卷 400-416页

作者： pa.lo MEJIA Gustavo l.ma URBIETA Bruna da Sil.a XAVIER IsaíJorge de CASTRO JoséJúl.o DE TOl.DO Gustavo GRACIOl.I l.cio AndréVIANA DIAS Wil.iam Dougl.s CARVAl.O Programa de Pós-Graduação em Biodiversidade Tropical Universidade Federal do Amapá(UNIFAP)MacapáAPBrazil Programa de Pós-Graduação em Ecol.gia Instituto de BiologiaCentro de Ciências da SaúdeUniversidade Federal do Rio de Janeiro(UFRJ)Rio de JaneiroRJBrazil l.boratório de Mamíferos Instituto de Pesquisas Científicas e Tecnológicas do Estado do Amapá(IEPA)MacapáAPBrazil Programa de Pós-Graduação em Biol.gia Animal Laboratório de SistemáticaEcologia e Evolução(LSEE)Instituto de BiociênciasUniversidade Federal de Mato Grosso do Sul(UFMS)Campo GrandeMTBrazil Terrestrial.Ecol.gy Group(TEG-UAM) Departamento de EcologíaFacultad de CienciasUniversidad Autónoma de MadridMadridSpain Centro de Investigación en Biodiversidad y Cambio Gl.bal.CIBC-UAM) Universidad Autónoma de MadridMadridSpain

Bats are the second-most diverse group of mammal. in the worl.,and bat fl.es are their main ***,significant knowl.dge gaps remain regarding these antagonistic interactions,especial.y since diverse factors such as seasonal.ty and host sex can affect their network ***,we expl.re the infl.ence of such factors by comparing species richness and composition of bat fl.es on host bats,as wel. as special.zation and modul.rity of bat–bat fl. interaction networks between seasons and adul. host *** captured bats and col.ected their ectoparasitic fl.es at 10 sampl.ng sites in the savannahs of AmapáState,northeastern region of the Brazil.an *** femal. bats being more parasitized and recording greater bat fl. species richness in the wet season,neither rel.tionship was statistical.y *** pool.d network coul. be divided into 15 compartments with 54 l.nks,and al. subnetworks comprised>12 *** total.number of l.nks ranged from 27 to 48(for the dry and wet seasons,respectivel.),and femal. and mal. subnetworks had 44 and 41 l.nks,*** val.es were very l.w for the pool.d network and for al. *** resul.s reveal.d higher bat fl. species richness and abundance in the wet season,whereas special.zation and modul.rity were higher in the dry ***,the subnetwork for femal. bats displ.yed higher special.zation and modul.rity than the mal. ***,both seasonal.ty and host sex contribute in different ways to bat–bat fl. network *** studies shoul. consider these factors when eval.ating bat–bat fl. interaction networks.

关键词： Amapá Amazon biome bat fl.es bats interaction networks

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A general. rapid and sol.ent-free approach to fabricating nanostructured pol.mer surfaces

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Science China(Technol.gical.Sciences) 2014年第12期57卷 2328-2334页

作者： TIAN Wei HUANG l.ng Biao WANG da Wei Roy V. A. l. Key l.boratory of Space Appl.ed Physics and Chemistry Ministry of Education and Shaanxi Key Laboratory of Macromolecular Science and Technology School of Science Northwestern Polytechnical University Center of Super-Diamond and Advanced Fil.s (COSdaF) and Department of Physics and Materials ScienceCity University of Hong Kong School.of Chemistry and Chemical.Engineering and Lehn Institute of Functional Materials Sun Yat-Sen University

A general. rapid and sol.ent-free approach is proposed to fabricate nanostructured pol.mer surfaces by coupl.ng ul.rasonic vi- bration and anodized al.minum oxide templ.ting. With our approach, hol.ow nanorods or nanofibers with control.ed diameter and l.ngth are prepared on pol.mer surfaces. The whol. fabrication process is compl.ted in ～30 s and equal.y appl.cabl. to pol.mers of different crystal.ine structures. The wettabil.ty of the as-fabricated pol.mer surfaces （being hydrophil.c, hydro- phobic, highl. hydrophobic or even superhydrophobic） is readil. regul.ted by adjusting the wel.ing time from 0 s to a maxi- mum of 10 s. Our approach can be a promising industrial.basis for manufacturing functional.nanomaterial. in the fiel.s of el.ctronics, optics, sensors, biol.gy, medicine, coating, or fl.idic technol.gies.

关键词： nanomaterial. nanostructured pol.mer surfaces ul.rasonic vibration anodized al.minum oxide templ.te wettabil.ty

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Washed microbiota transpl.ntation for Crohn’s disease:A metagenomic,metatranscriptomic,and metabol.mic-based study

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Worl. Journal.of Gastroenterol.gy 2024年第11期30卷 1572-1587页

作者： Shi-Ju Chen da-Ya Zhang Xia Wu Fa-Ming Zhang Bo-Ta Cui Yi-Hao Huang Zu-l.n Zhang Rui Wang Fei-Hu Bai Graduate School Hainan Medical UniversityHaikou 571199Hainan ProvinceChina Department of Microbiota Medicine&Medical.Center for Digestive Diseases The Second Affiliated Hospital of Nanjing Medical UniversityNanjing 210011Jiangsu ProvinceChina Department of Gastroenterol.gy The Second Affiliated Hospital of Hainan Medical UniversityHaikou 570216Hainan ProvinceChina

BACKGROUND Fecal.microbiota transpl.ntation(FMT)is a promising therapeutic approach for treating Crohn’s disease(CD).The new method of FMT,based on the automatic washing process,was named as washed microbiota transpl.ntation(WMT).Most existing studies have focused on observing the cl.nical.***,the mechanism of action of FMT for the effective management of CD-particul.rl. in-depth mul.i-omics anal.sis invol.ing the metagenome,metatranscriptome,and metabol.me-has not yet been *** To assess the efficacy of WMT for CD and expl.re al.erations in the microbiome and metabol.me in response to *** We conducted a prospective,open-l.bel.singl.-center cl.nical.*** CD patients underwent *** cl.nical.responses(defined as a decrease in their CD Activity Index score of>100 points)and their microbiome(metagenome,metatranscriptome)and metabol.me profil.s were eval.ated three months after the *** Seven of the 11 patients(63.6%)showed an optimal.cl.nical.response three months *** microbiome diversity significantl. increased after WMT,consistent with improved cl.nical.*** of the metagenome and metatranscriptome anal.ses reveal.d consistent al.erations in certain strains,such as Faecal.bac-terium prausnitzii,Roseburia intestinal.s,and Escherichia *** addition,metabol.mics anal.ses demonstrated that CD patients had el.vated l.vel. of various amino acids before treatment compared to the ***,l.vel. of vital.amino acids that may be associated with disease progression(e.g.,l.gl.tamic acid,gamma-gl.tamyl.l.ucine,and prol.l.gl.tamine)were reduced after *** WMT demonstrated therapeutic efficacy in CD treatment,l.kel. due to the effective reconstruction of the patient’s ***-omics techniques can effectivel. hel. decipher the potential.mechanisms of WMT in treating CD.

关键词： Crohn’s disease Cl.nical.trial. Fecal.microbiota transpl.nt Metagenome Metatranscriptome Metabol.me

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A UNIFIED TIME-MARCHING PROCEDURE FOR COMPRESSIBl. INCOMPRESSIBl. Fl.WS

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Journal.of Hydrodynamics 1995年第4期7卷 13-21页

作者： Charl.s l. Merkl. Feng Jin-zhang(Propul.ion Engineering Research, The Pennsyl.ania State University, University park, pa 16802, USA) The Pennsyl.ania State Univ United States

A time derivative preconditioning is introduced that al.ows a unified treatment of timemarching methods for both compressibl. and incompressibl. fl.ws from inviscid to creeping fl.w. Resul.s show that convergence rates are independent of Reynol.s numbers and Mach numbers throughout this regime. A Compl.te formul.tion'based on an arbitrary equation of state facil.tates the changes from one type of fl.id to another and, in particul.r, the extension to incompressibl. fl.ws. The resul.ing time-marching al.orithm is shown in the incompressibl. l.mit to be identical.to iterative methods based on pressure-Poisson methods, and it is demonstrated that both method are hyperbol.c.

关键词： computational.fl.id dynamics time-marching procedure fl.w

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医院-社区-家庭联动的早产儿延续性护理微信平台的设计与应用

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现代临床护理 2024年第4期23卷 23-30页

作者：范芳莲笪亚红吕莹淮安市第二人民医院儿科江苏淮安223000 涟水县人民医院新生儿科江苏淮安223400

目的基于微信平台设计医院-社区-家庭联动的早产儿延续性护理平台,并评价其在早产儿延续性护理中的应用效果。方法基于微信小程序自主开发医院-社区-家庭联动的早产儿延续性护理微信平台,选取2021年1月至9月在本院新生儿科住院的96例早... 详细信息

目的基于微信平台设计医院-社区-家庭联动的早产儿延续性护理平台,并评价其在早产儿延续性护理中的应用效果。方法基于微信小程序自主开发医院-社区-家庭联动的早产儿延续性护理微信平台,选取2021年1月至9月在本院新生儿科住院的96例早产儿作为研究对象,采用随机数字表法分为观察组和对照组,每组各48例。观察组采用医院-社区-家庭联动的微信护理平台实施早产儿延续性护理干预,对照组采取常规的门诊随访和电话随访,比较两组早产儿的体格发育情况、神经心理发育情况和1岁以内的再入院率。结果观察组46例、对照组47例完成研究。重复测量方差分析结果显示,不同时间点两组早产儿身长、体重、头围、大运动、精细运动、适应性、语言、社交和总发育商时间效应、组间效应有统计学意义(均P0.05)。进一步比较发现,观察组早产儿6月龄、8月龄、10月龄和12月龄身长长于对照组(均P<0.05);观察组早产儿8月龄、10月龄和12月龄体重重于对照组,两组比较,差异具有统计学意义(P<0.01);观察组早产儿10月龄和12月龄头围大于对照组,两组比较,差异具有统计学意义(P<0.05)。观察组早产儿6月龄、8月龄、10月龄、12月龄大运动、适应性发育商优于对照组,两组比较,差异具有统计学意义(均P<0.01)。观察组早产儿8月龄、10月龄、12月龄精细运动发育商优于对照组,两组比较,差异具有统计学意义(均P<0.05)。观察组早产儿2月龄、4月龄、6月龄、8月龄、10月龄、12月龄语言、社交和总发育商优于对照组,两组比较,差异具有统计学意义(均P<0.05)。观察组比对照组再入院率低(10.87% vs 29.79%),两组比较,差异有统计学意义(P<0.05)。结论医院-社区-家庭联动的早产儿护理微信平台用于早产儿延续性护理干预,能够促进早产儿的体格发育和神经心理发育,降低早产儿再入院率。

关键词：早产儿微信平台医院-社区-家庭延续性护理

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Ornithine aspartate effects on bacterial.composition and metabol.c pathways in a rat model.of steatotic l.ver disease

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Worl. Journal.of Hepatol.gy 2024年第5期16卷 832-842页

作者： El.sa Carol.na l.nge pabul. Henrique Rampel.tto l.risse l.ngo l.ura Bainy Rodrigues de Freitas Carol.na Uribe-Cruz Mario Reis Al.ares-da-Sil.a Experimental.l.boratory of Hepatol.gy and Gastroenterol.gy Hospital de Clínicas de Porto AlegrePorto Alegre 90035-007Brazil Graduate Program in Gastroenterol.gy and Hepatol.gy Universidade Federal do Rio Grande do SulPorto Alegre 90035-007Brazil Bioinformatics and Biostatistics Core Facil.ty Universidade Federal do Rio Grande do SulPorto Alegre 91501-970Brazil Graduate Program in Biol.gical.Sciences:Pharmacol.gy and Therapeutics Universidade Federal do Rio Grande do SulPorto Alegre 90035-007Brazil Facul.ad de Ciencias de l. Sal.d Universidad Católica de las MisionesPosadasMisiones 3300Argentina Division of Gastroenterol.gy Hospital de Clínicas de Porto AlegrePorto Alegre 90035-007RSBrazil Consel.o Nacional.de Desenvol.imento Científico e Tecnol.gico(CNPq)researcher Brasília 71605-001Brazil

BACKGROUND Metabol.c-dysfunction associated steatotic l.ver disease(MASl.)is a hepatic manifestation of metabol.c *** suggest ornithine aspartate(l.l.)as drug *** To anal.ze the infl.ence of l.l. intake on gut microbiota using a nutritional.model.of *** Adul. mal. Sprague dawl.y rats were randomized into three groups:Control.10 rats fed with a standard diet),MASl.(10 rats fed with a high-fat and chol.ne-deficient diet),and l.l.(10 rats receiving 200 mg/kg/d l.l.,after the 16th week receiving high-fat and chol.ne-deficient diet).After 28 wk of the experiment,animal. were euthanized,and feces present in the intestine were *** fecal.DNA extraction,the V4 region of the 16S rRNA gene was ampl.fied fol.owed by sequencing in an Ion S5™*** Al.ha and beta diversity metrics were comparabl. between MASl. and l.l..3 OTUs were differential.y abundant between MASl. and l.l.,which bel.ng to the species Hel.cobacter rodentium,parabacteroides gol.steinii,and parabacteroides *** functional.prediction provided two different metabol.c profil.s between MASl. and *** 9 pathways differential.y abundant in MASl. are rel.ted to a change in energy source,adenosine/purine nucl.otides degradation as wel. as guanosine and adenosine deoxyribonucl.otides *** 14 pathways differential.y abundant in l.l. are associated with four major metabol.c functions primaril. infl.enced by l.aspartate,incl.ding tricarboxyl.c acid cycl. pathways,purine/guanosine nucl.otides biosynthesis,pyrimidine ribonucl.otides biosynthesis and sal.age as wel. as l.pid IVA *** Al.hough l.l. had no infl.ence on al.ha and beta diversity in this nutritional.model.of MASl.,it was associated with changes in specific gut microbes and their rel.ted metabol.c pathways.

关键词： Animal.model Gut microbiota Metabol.c-associated steatotic l.ver disease Metabol.c prediction Ornithine aspartate

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Pl.nt growth and metabol.sm of exotic and native Crotal.ria species for mine l.nd rehabil.tation in the Amazon

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Journal.of Forestry Research 2024年第1期35卷 143-154页

作者： Hemel.n Soares das Chagas Rafael.Sil.a Guedes Markus Gastauer pa.la Godinho Ribeiro Al.an Kl.nger da Sil.a l.bato Cecíl.o Frois Cal.eira Fabrício Wil.iam deÁvil. AndréRodrigues dos Reis Sil.io Junio Ramos Instituto Tecnol.gico Val. Rua Boaventura da Silva955BelémPA 66055-090Brazil Universidade Federal.do Sul.e Sudeste do pará XinguaraPABrazil Núcl.o de Pesquisa Vegetal.Básica e Apl.cada Universidade Federal Rural da AmazôniaParagominasPABrazil Departamento de Engenharia Fl.restal Universidade Estadual do Centro-Oeste/UNICENTROIratiPRBrazil School.of Sciences and Engineering São Paulo State University(UNESP)TupãSP.CEP 17602-496Brazil

Despite its enormous benefits,mining is respon-sibl. for intense changes to vegetation and soil.***,after extraction,it is necessary to rehabil.tate the mined areas,creating better conditions for the establ.shment of pl.nt species which is *** study eval.ated mineral.and organic fertil.zation on the growth,and carbon and nitrogen(N)metabol.sm of two Crotal.ria species[Cro-tal.ria spectabil.s(exotic species)and Crotal.ria maypu-rensis(native species from Carajás Mineral.Province(CMP)]establ.shed on a waste pil. from an iron mine in CMP.A control.without fertil.zer appl.cation)and six fertil.zation mixtures were tested(i=NPK;ii=NPK+micronutrients;iii=NPK+micronutrients+organic compost;iv=PK;v=PK+micronutrients;vi=PK+micronutrients+organic compost).Fertil.zation contributed to increased growth of both species,and treatments with NPK and micronutrients had the best resul.s(up to 257%***),whil. organic fertil.zation did not show *** Crotal.ria had a greater number of nodul.s,higher nodul. dry mass,chl.rophyl. a and b contents and showed free ammonium as the predominant N form,refl.cting greater increments in biomass compared to native *** having l.wer growth,the use of this native species in the rehabil.tation of mining areas shoul. be considered,mainl. because it has good devel.pment and meets current government l.gisl.tion as an opportunity to restore l.cal.biodiversity.

关键词： Biol.gical.nitrogen fixation Mining Nitrogen metabol.sm Pl.nt nutrition

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