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Differential Expression of Rice Valine-Qlutamine Gene Family in Response to Nitric Oxide and Regulatory Circuit of OsVQ7 and OsWRKY24

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Rice science 2020年第1期27卷 10-20页

作者： peng xixu XIAO Ting MENG Jiao TAO Zong ZHOU Dinggang TANG Xinke WANG Haihua School of Life Science Hunan University of Science and TechnologyXiangtan 411201China Key Laboratory of Integrated Management of the Pests and Diseases on Horticultural Crops in Hunan Province Xiangtan 411201China Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted Soils College of Hunan ProvinceXiangtan 411201China

The functional diversity of plant valine-qlutamine(VQ) proteins is closely associated with their partners WRKY transcription factors, and also with a complex network of signaling pathways that mediated by hormone molecules. We reported genome-wide expression profiles of differentially expressed rice VQ genes under nitric oxide(NO) treatment based on a microarray analysis. Cluster analysis of expression patterns revealed that some VQ genes and WRKY genes shared similar expression trends. Prediction of cis-elements showed that W-box or W-box-like sequences were overrepresented within the promoters of most of NO-responsive VQ genes. In particular, the similarly expressed Os VQ7 and Os WRKY24 showed great induction upon NO triggering. Transient expression assay and chromatin immunoprecipitation analysis demonstrated that OsWRKY24 was specifically bound to the promoter regions of Os VQ7 and Os WRKY24 itself, which contain multiple copies of W-box or W-box-like cis-elements. Yeast-two-hybrid assay indicated that OsWRKY24 can interact physically with OsVQ7 through the C-terminal of WRKY domain. The results suggested that OsVQ7 and OsWRKY24 may form an auto-and cross-regulation circuit that is required for tight regulation and fine-tuning of physiological processes they are involved in. These findings provided a solid foundation for exploring the specific functions of the VQ protein family in NO signaling pathway.

关键词： rice valine-qlutamine protein expression profile WRKY transcription factor nitric oxide regulatory circuit

First detection of Diaporthe eucommiae causing leaf black blight disease on Cyclocarya paliurus

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First detection of Diaporthe eucommiae causing leaf black bl...

Differential expression of rice VQ protein gene family in response to nitric oxide and the regulatory circuit of VQ7 and WRKY24

中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： Jiang Dan He Huanhuan peng xixu Tao Zong Xiao Ting Wang Haihua School of Life Science Hunan University of Science and Technology Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-polluted Soils College of Hunan Province

During the vegetation period(March to October) of 2013 and 2014,an outbreak of leaf black blight was observed on Cyclocarya paliurus plants in three plantations in Chengbu County,Hunan Province,China.A Diaporthe species was consistently isolated from the diseased leaflets or *** on the morphological characteristics of colony appearance,shape of conidia and conidiomata as well as sequences of internal transcribed spacer regions(ITS),the fungus was identified as Diaporthe *** test showed that *** isolates caused the symptoms originally observed on those of naturally infected plants,and the pathogenic isolates were successfully re-isolated from inoculated leaflets,thus fulfilling Koch’s *** is the first report of *** causing leaf black blight on *** screening indicated that the *** isolates were highly sensitive to thiophanate-methyl,chlorothalonil and iprodione,suggesting that practical use of these three fungicides has high potentials to control the leaf black blight on ***.

关键词： Diaporthe eucommiae Leaf black blight Identification of pathogen Cyclocarya paliurus

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Differential expression of rice VQ protein gene family in re...

First Report of Alternaria alternata Causing Leaf Spot of Tartary Buckwheat in China

中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： Wang Haihua Xiao Ting Meng Jiao Tao Zong peng xixu Zhou Dinggang Tang Xinke School of Life Science Hunan University of Science and Technology

The functional diversity of plant-specific VQ proteins are closely associated with their partners WRKY transcription factors,and with a complex network of signaling pathways that mediated by hormone ***,no information is available about the expression patterns of VQ genes modulated by nitric oxide(NO),thus posing a hindrance to defining the mechanisms underlying the involvement of VQ proteins in NO signaling ***,we report genome-wide expression profiles of the differentially expressed rice VQ genes under NO treatment based on a microarray *** analysis of expression patterns revealed that some VQ genes and WRKY genes share similar expression *** of cis-elements shows that W-box,or W-box like sequences are overrepresented within the promoters of most of the NO-responsive VQ *** particular,the similarly expressed VQ1 and WRKY24 showed great induction upon NO *** expression assay demonstrated that WRKY24 specifically bound to the promoter regions of VQ1 and WRKY2A itself,which contain a multiple of copies of W-box or W-box like ***-two-hybrid assay indicated WRKY24 could interact physically with VQ7 through the C-terminal WRKY ***,VQ7 and WRKY24 formed an autoand cross-regulation circuit that is required for tight regulation and fine-tuning of physiological processes they are involved *** findings provide a solid foundation for exploring the specific functions of VQ protein family in NO signaling pathway.

关键词： VQ proteins Expression profiles WRKY transcription factors Nitric oxide Regulatory circuit Rice

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First Report of Alternaria alternata Causing Leaf Spot of Ta...

响应镉胁迫的水稻WRKY15转录因子基因的分离与表达特征

中国植物病理学会2021年学术年会

作者： LI Shaoqing SHEN Quan Wang Haihua HE Feng XIAO Zuyin peng xixu ZhOU Meiliang TANG Xinke School of Life Science Hunan University of Science and Technology Key Laboratory of Genetic Improvement and Multiple Utilization of Economic Crops in Hunan Province Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-polluted Soils College of Hunan Province Institute of Crop Science Chinese Academy of Agricultural Sciences

Buckwheat(Fagopyrum tataricum) is recognized as a healthy food with abundant nutrients and high levels of *** April and May of 2020,an unknown tartary buckwheat leaf spot distinct from Nigrospora leaf spot was observed in Xiangxiang,Hunan,China(27°49′54″N,112°18′48″E.).Disease incidence was 60%-70% within three fields(totally 7 000 m).The disease occurred after plants *** symptoms began as circular,or ellipsoid,chlorotic,water-soaked spots,mostly on leaf apexes or leaf *** small spots gradually enlarged and often coalesced to form large circular or irregular,pale to light brown lesions,and the infected leaves eventually withered and fell *** 2 mm × 2 mm infected tissue pieces collected from five locations were sterilized in 70% ethanol for 10 s,in 2% NaClO for 30 s,rinsed in sterile water for three times,dried,and placed on PDA with lactic acid(3 mL/L).After 3-5 days at 28 ℃ in the dark,17 fungal isolates were purified using single-spore isolation *** all fungal isolates had similar *** were initially olive green with white margin and later turned dark olive or black with profuse *** were borne in long chains,tawny to brownish green,with 1-3 longitudinal and 1-7 transverse septa,pyriform,and measured 9.5-39.6 μm long,and 5.1-12.6 μm wide(n=50).Based on morphological characteristics,the fungus was identified as Alternaria *** internal transcribed spacer(ITS),glyceraldehyde-3-phosphate dehydrogenase(GAPDH),translation elongation factor 1-α(TEF) and Alternaria major allergen(Alt al) genes of isolate BLS-1 were amplified using ITS1/ITS4,EF1-728 F/EF1-986 R,Gpdl/Gpd2 and Alt-4 for/Alt-4 rev,*** were deposited into GenBank with *** MW453091(ITS),MW480219(GAPDH),MW480218(TEF),and MW480220(Alt al).BLASTn analysis showed 99.8%(ITS,MH854758.1),100%(GAPDH,KP124155.1),99.8%(TEF,KP125073.1) and 100%(Alta1,KP123847.1) identity with reference strain CBS 106.24 of ***

关键词： Tartary buckwheat(Fagopyrum tataricum) leaf Spot Alternaria alternata pathogen identification

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中国水稻科学 2018年第2期32卷 103-110页

作者：彭喜旭白宁宁王海华湖南科技大学生命科学学院湖南湘潭411201 重金属污染土壤生态修复与安全利用湖南省高校重点实验室湖南湘潭411201

【目的】镉(Cd)是一种对植物生长发育和人类健康均具有很强毒害效应的重金属元素。WRKY转录因子在抗逆生理过程中起重要的调节作用。为了研究WRKY基因在镉胁迫响应和抗性反应中的作用,【方法】基于基因注释,采用RT-PCR策略从一氧化氮(NO... 详细信息

【目的】镉(Cd)是一种对植物生长发育和人类健康均具有很强毒害效应的重金属元素。WRKY转录因子在抗逆生理过程中起重要的调节作用。为了研究WRKY基因在镉胁迫响应和抗性反应中的作用,【方法】基于基因注释,采用RT-PCR策略从一氧化氮(NO)处理的水稻叶片中分离完整的Os WRKY15编码序列,运用生物信息学软件分析其编码蛋白的结构特征,采用q RT-PCR分析其基因的空间(器官)特异性及诱导表达模式;运用酵母单杂交方法检测其转录激活活性。【结果】克隆了Os WRKY15 c DNA序列,长988 bp,包含一个长825 bp的完整开放读码框,编码274个氨基酸。Os WRKY15具有1个典型的WRKY保守结构域,锌指类型为C2HC,归类于WRKY第3组;拥有一个核定位信号,因此推测为一种核蛋白。酵母单杂交分析表明,它具有转录激活活性。Os WRKY15在根中的表达丰度最高,其次是叶和茎,在颖果、花和干种子中的表达丰度较低;受Cd、NO和脱落酸(ABA)快速且显著诱导,且在根中被诱导的程度比叶中大,而水杨酸、茉莉酸和乙烯对其表达无明显影响。【结论】Os WRKY15是一个具有功能的核蛋白,推测其可能通过NO、ABA介导的信号途径参与水稻对Cd胁迫的抗性反应。

关键词： WRKY转录因子镉基因分离基因表达模式水稻

Time-Periodic Solutions to Quasilinear Hyperbolic Systems on General Networks

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Chinese Annals of Mathematics,Series B 2024年第1期45卷 41-72页

作者： peng QU School of Mathematical Sciences Fudan UniversityShanghai 200433China

For quasilinear hyperbolic systems on general networks with time-periodic boundary-interface conditions with a dissipative structure,the existence and stability of the time-periodic classical solutions are discussed.

关键词： Time-periodic solution Quasilinear hyperbolic system General net-work Classical solution Asymptotic stability

Thermal and mechanical behavior of casting copper alloy wheel during wheel and belt continuous casting process

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China Foundry 2024年第1期21卷 82-90页

作者： Kun Gao Yan peng School of Mechanical Engineering Yanshan UniversityQinhuangdao 066004HebeiChina

To investigate the thermal and mechanical behavior of casting wheel,a two-dimensional thermoelastic-plastic finite element model was used to predict the temperature,stress and distortion distribution of the casting wheel during the wheel and belt continuous casting *** effects of grinding thickness and casting speed on the thermal and mechanical behaviors of the center of the hot face of the casting wheel were discussed in *** each rotation,the casting wheel passes through four different spray *** results show that the temperature distribution of the casting wheel in different spray zones is similar,the temperature of the hot face is the highest and the temperature reaches the peak in the spray zoneⅢ.The stress and distortion depend on the temperature distribution,and the maximum stress and distortion of the hot face are 358.2 MPa and 1.82 mm,*** temperature at the center of the hot face decreases with increasing grinding thickness and increases with increasing casting speed.

关键词： casting wheel finite element model grinding thickness casting speed hot face spray zones

Numerical simulation of fluid flow and free surface fluctuations during wheel and belt casting process

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Journal of Iron and Steel Research International 2024年第5期31卷 1117-1126页

作者： Kun Gao Yan peng National Engineering Research Center for Equipment and Technology of Cold Strip Rolling Yanshan UniversityQinhuangdao066004HebeiChina

The unstable fluid flow and severe free surface fluctuations in the wheel and belt caster can affect the quality of the cast *** lower level height tends to entrap inclusions in the molten *** the other hand,the higher level height makes the production process more dangerous due to the overflow of high temperature fluid from the mold.A computational model of the molten metal pouring process was *** transient fluid flow and free surface fluctuations behavior were calculated using the three-dimensional large eddy simulation model and the volume of fluid *** results show that the flow velocity of the main jet gradually decreases under the influence of the low kinetic energy fluid in the *** is an obvious oscillation in the tail of the jet,while the flow field is asymmetric in *** jet is closer to the inside radius side due to the Coanda effect,and there is a recirculation zone on the inside radius and the outside radius respectively,according to the 10 s time-averaged *** with the industrial observation and simulation results,the shape of the free surface is a wave that varies with *** addition,the free surface height is lowest and the flow velocity is highest in the region near the jet.

关键词： Fluid flow Free surface Wheel and belt caster Large eddy simulation Volume of fluid method

Two-Stage Online Debiased Lasso Estimation and Inference for High-Dimensional Quantile Regression with Streaming Data

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Journal of Systems Science & Complexity 2024年第3期37卷 1251-1270页

作者： peng Yanjin WANG Lei School of Statistics and Data Science KLMDASRLEBPS and LPMCNankai UniversityTianjin 300071China

In this paper,the authors propose a two-stage online debiased lasso estimation and statistical inference method for high-dimensional quantile regression(QR)models in the presence of streaming *** the first stage,the authors modify the QR score function based on kernel smoothing and obtain the online lasso smoothed QR estimator through iterative *** estimation process only involves the current data batch and specific historical summary statistics,which perfectly accommodates to the special structure of streaming *** the second stage,an online debiasing procedure is carried out to eliminate biases caused by the lasso penalty as well as the accumulative approximation error so that the asymptotic normality of the resulting estimator can be *** authors conduct extensive numerical experiments to evaluate the performance of the proposed *** experiments demonstrate the effectiveness of the proposed method and support the theoretical *** application to the Beijing PM2.5 Dataset is also presented.

关键词： Adaptive tuning asymptotic normality debiased lasso online updating quantile regres-sion