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Comparison between gene expression of conidia and germinating phase in Trichophyton rubrum

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Science china(Life sciences) 2007年第3期50卷 377-384页

作者： yang Li1,2, WANG LingLing2, peng junping2, YU Lu2, LIU Tao2, LENG WenChuan2, yang jian2, CHEN LiHong2 , ZHANG WenLiang2, ZHANG qian2, qi Yipeng1 & jin qi1,2,3 1 college of Life sciences, Wuhan University, Wuhan 430072, china 2 state key laboratory for molecular virology and genetic engineering, chinese Center for Disease Control and Prevention, beijing 100176, china 3 institute of pathogen biology, chinese academy of medical sciences, beijing 100730, china 1. college of Life sciences Wuhan University Wuhan 430072 China 2. state key laboratory for molecular virology and genetic engineering Chinese Center for Disease Control and Prevention Beijing 100176 China 3. institute of pathogen biology Chinese Academy of Medical Sciences Beijing 100730 China

Trichophyton rubrum is a dominating superficial dermatophyte, whose conidial germination is corre- lated to pathopoiesis and a highly important developmental process. To investigate the changes of physiology, biochemistry and cytology during the germination, we selected 3364 function identified ESTs from T. rubrum cDNA library to construct cDNA microarrays, and compared the gene expression levels of conidia and germinating phase. Data analysis indicated that 335 genes were up-regulated during the germination, which mainly encoded translated, modified proteins and structural proteins. The constituents of cell wall and cell membrane were synthetized abundantly, suggesting that they are the foundation of cell morphogenesis. The ingredients of the two-component signal transduction sys- tem were up-regulated, presuming that they were important for the conidial germination. Genes of various metabolic pathways were expressed prosperously, especially the genes that participated in glycolysis and oxidative phosphorylation were up-regulated on the whole, demonstrating that in the environment with sufficient oxygen and glucose, conidia obtained energy through aerobic respiration. This paper provides important clues which are helpful to understanding the changes in gene expres- sion, signal conduction and metabolism characteristics during T. rubrum conidial germination, and possess significant meaning to the study of other superficial dermatophytes.

关键词： Comparison between gene expression of conidia and germinating phase in Trichophyton rubrum gene cDNA

Global transcriptional profiles of Trichophyton rubrum in response to Flucytosine

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Science china(Life sciences) 2009年第12期52卷 1173-1185页

作者： ZHAO Rong BIN Wen DIAO Youjiang yang jian LIU Tao peng junping jin qi state key laboratory for molecular virology and genetic engineering National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 100176China institute of pathogen biology Chinese Academy of Medical SciencesBeijing 100730China

Trichophyton rubrum (***) is one of the most common human fungal pathogens that cause chronic infections of the skin and nails. To identify antifungal responsive genes, cDNA microarray analysis was performed for T. rubrum to reveal global transcriptional profiles of drug-specific responses to 5-Flucytosine (5-FC). cDNA microarray was constructed from the T. rubrum expressed sequence tag (ESTs) database, the minimum inhibitory concentration (MIC) of 5-FC was determined, and microarray hybridization and data analysis were applied. The expression pattern of 7 genes observed by microarray was confirmed by the quantitative real-time reverser transcription polymerase chain reaction (RT-PCR). Data analysis indicated that a total of 474 genes were found differentially expressed, 196 showed an increase in expression and 278 showed a decrease in expression. Marked down-regulation of genes involved in nucleotide metabolism (such as CDC21), transcription (such as E2F1), and RNA processing (such as SGN1, RIM4 and NOP1) was observed. Other genes involved in signal transduction, chaperones, inorganic ion transport, secondary metabolite biosynthesis, amino acid transport, lipid transport and potential drug resistance mechanism were also affected by 5-FC. Quantitative real-time RT-PCR of the selected genes confirmed the reliability of the microarray results. This is the first analysis of transcriptional profiles in response to 5-FC for T. rubrum. The findings may be valuable for the identification of genes involved in mechanisms of action and mechanisms of antifungal drug resistance of 5-FC.

关键词： Trichophyton rubrum cDNA microarray 5-Flucytosine gene expression

Comparative analysis of whole genome structure of Streptococcus suis using whole genome PCR scanning

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Science china(Life sciences) 2008年第1期51卷 21-26页

作者： XIONG ZhaoHui WEI CanDong yang jian peng junping XU XingYe WANG Yu jin qi state key Lab for molecular virology and genetic engineering Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 100176China chinese Center for Disease Control and Prevention Beijing 100050China institute of pathogen biology Chinese Academy of Medical SciencesBeijing 100730China

An outbreak associated with Streptococcus suis infection in humans emerged in Sichuan province, china in 2005. The outbreak is atypical for the apparent large number of human cases, high fatality rate and geographical spread. To determine whether the bacterium has changed, we compared both human and animal isolates from the Sichuan outbreak with those collected previously within china and in other countries using whole genome PCR scanning (WGPScaning) comparative sequencing of several known virulence factor genes and multilocus sequence typing (MLST) analysis. WGPScanning analysis showed that all primer pairs yielded PCR products of the expected sizes in all four strains tested. The nucleotide sequences of all the detected virulence factor genes are identical in the four strains and MLST results showed that the four isolates studied and reference strain all belonged to the ST1 com-plex. No new genetic changes were found in the genome structure of the isolates from this Sichuan outbreak.

关键词： Streptococcus suis whole genome PCR scanning multilocus sequence typing(MLST)