Comparative analysis of whole genome structure of Streptococcus suis using whole genome PCR scanning

作     者：XIONG ZhaoHui WEI CanDong YANG Jian PENG JunPing XU XingYe WANG Yu JIN Qi 

作者机构：State Key Lab for Molecular Virology and Genetic EngineeringInstitute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 100176China Chinese Center for Disease Control and PreventionBeijing 100050China Institute of Pathogen BiologyChinese Academy of Medical SciencesBeijing 100730China 

出 版 物：《Science China(Life Sciences)》 (中国科学(生命科学英文版))

年 卷 期：2008年第51卷第1期

页      面：21-26页

核心收录：

学科分类：07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 090102[农学-作物遗传育种] 0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 0901[农学-作物学] 0836[工学-生物工程] 10[医学] 

基　　金：Supported by the National Key Technologies Research and Development Program (Grant No. 2005BA711A09) from the Ministry of Science and Technology of China 

主　　题：Streptococcus suis whole genome PCR scanning multilocus sequence typing(MLST) 

摘      要：An outbreak associated with Streptococcus suis infection in humans emerged in Sichuan province, China in 2005. The outbreak is atypical for the apparent large number of human cases, high fatality rate and geographical spread. To determine whether the bacterium has changed, we compared both human and animal isolates from the Sichuan outbreak with those collected previously within China and in other countries using whole genome PCR scanning (WGPScaning) comparative sequencing of several known virulence factor genes and multilocus sequence typing (MLST) analysis. WGPScanning analysis showed that all primer pairs yielded PCR products of the expected sizes in all four strains tested. The nucleotide sequences of all the detected virulence factor genes are identical in the four strains and MLST results showed that the four isolates studied and reference strain all belonged to the ST1 com-plex. No new genetic changes were found in the genome structure of the isolates from this Sichuan outbreak.